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The map label for this gene is cbiE [H]

Identifier: 153935805

GI number: 153935805

Start: 1014674

End: 1015324

Strand: Direct

Name: cbiE [H]

Synonym: CLC_0992

Alternate gene names: 153935805

Gene position: 1014674-1015324 (Clockwise)

Preceding gene: 153937445

Following gene: 153936317

Centisome position: 26.98

GC content: 26.11

Gene sequence:

>651_bases
ATGAATACTATACATAAGAATCTACAATTTAAAAAGATTTACATAGTAGGCTTAGGGCCAGGACATAAAGATTATATGTT
AAGTGGTGCTGTAAACAATTTAGAAAAAGTAGATATAATAATAGGATTTAAAAGAGCGATAGAAAGCTTAGATTTCATAA
AAAATAATAAAAAAGTAGTAAATAAAATAAGTGGAATATTAGATTATATAAAAGAAAATAAGGATAAAAACATATCTATA
GTAGCCTCTGGAGATCCTTGTTTTTATGGTATAAGCAATTATATTAAAAACAATTATGAAGGCAAAATAGAGGTAATTCC
AGGCATAAGTTCTTACCAATATATGATGGCAAAAATCAATGAAAGTTGGCAAAATAGTTTTTTAGGAAGTCTTCATGGTA
GAGAAGAAGAGTTCATAGAAAAAGTTAAAAGTTATGAAAAAACCATTTGGCTTACAGATAAAAAGAATAGTCCAGATAAA
TTATGTAAAAGATTAATAGAAAATAACATAGAAGCAGAGGTAATAGTGGGAGAAAATCTATCTTATAAGGATGAAAGGAT
AATTAAAGGAAATCCACAAGAATTAGAAAATATGAGATTTTCAGATTTAACAGTGGTGTATATAAGAGTTAATTCTGAAA
TGAATGTTTAA

Upstream 100 bases:

>100_bases
ATCCTTTAAATAAATATAAAAAATTAAAAGCGTTGTAGAAAGAAAATTTATATAGAACTATCTACCGTTCAATGTTCAAT
ATGGACGAGGGGGGATTTTG

Downstream 100 bases:

>100_bases
GATTTAATTGAAGATGTACAATAGCAGATTAATGCGTAATAAACATCATTTATATTGATTGTAAAGAATACTTTAATGAA
TGGAGAAGAAAATGAAACAA

Product: precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit

Products: S-adenosyl-L-homocysteine; Precorrin 8X; CO2

Alternate protein names: Cobalt-precorrin-6 methyltransferase; Cobalt-precorrin-6Y methylase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNTIHKNLQFKKIYIVGLGPGHKDYMLSGAVNNLEKVDIIIGFKRAIESLDFIKNNKKVVNKISGILDYIKENKDKNISI
VASGDPCFYGISNYIKNNYEGKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKTIWLTDKKNSPDK
LCKRLIENNIEAEVIVGENLSYKDERIIKGNPQELENMRFSDLTVVYIRVNSEMNV

Sequences:

>Translated_216_residues
MNTIHKNLQFKKIYIVGLGPGHKDYMLSGAVNNLEKVDIIIGFKRAIESLDFIKNNKKVVNKISGILDYIKENKDKNISI
VASGDPCFYGISNYIKNNYEGKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKTIWLTDKKNSPDK
LCKRLIENNIEAEVIVGENLSYKDERIIKGNPQELENMRFSDLTVVYIRVNSEMNV
>Mature_216_residues
MNTIHKNLQFKKIYIVGLGPGHKDYMLSGAVNNLEKVDIIIGFKRAIESLDFIKNNKKVVNKISGILDYIKENKDKNISI
VASGDPCFYGISNYIKNNYEGKIEVIPGISSYQYMMAKINESWQNSFLGSLHGREEEFIEKVKSYEKTIWLTDKKNSPDK
LCKRLIENNIEAEVIVGENLSYKDERIIKGNPQELENMRFSDLTVVYIRVNSEMNV

Specific function: Probably catalyzes the methylation of C-5 in cobalt- precorrin-6Y to form cobalt-precorrin-7W-a [H]

COG id: COG2241

COG function: function code H; Precorrin-6B methylase 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR012818 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.132; 1.-.-.-

Molecular weight: Translated: 24854; Mature: 24854

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIHKNLQFKKIYIVGLGPGHKDYMLSGAVNNLEKVDIIIGFKRAIESLDFIKNNKKVV
CCCCCCCCEEEEEEEEEECCCCCCEEECCCCCCCEEEEEEEEHHHHHHHHHHHHCCHHHH
NKISGILDYIKENKDKNISIVASGDPCFYGISNYIKNNYEGKIEVIPGISSYQYMMAKIN
HHHHHHHHHHHCCCCCCEEEEECCCCCEECCHHHHCCCCCCEEEEECCCCHHHHHHHHHH
ESWQNSFLGSLHGREEEFIEKVKSYEKTIWLTDKKNSPDKLCKRLIENNIEAEVIVGENL
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCC
SYKDERIIKGNPQELENMRFSDLTVVYIRVNSEMNV
CCCCCEEECCCHHHHHCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MNTIHKNLQFKKIYIVGLGPGHKDYMLSGAVNNLEKVDIIIGFKRAIESLDFIKNNKKVV
CCCCCCCCEEEEEEEEEECCCCCCEEECCCCCCCEEEEEEEEHHHHHHHHHHHHCCHHHH
NKISGILDYIKENKDKNISIVASGDPCFYGISNYIKNNYEGKIEVIPGISSYQYMMAKIN
HHHHHHHHHHHCCCCCCEEEEECCCCCEECCHHHHCCCCCCEEEEECCCCHHHHHHHHHH
ESWQNSFLGSLHGREEEFIEKVKSYEKTIWLTDKKNSPDKLCKRLIENNIEAEVIVGENL
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCC
SYKDERIIKGNPQELENMRFSDLTVVYIRVNSEMNV
CCCCCEEECCCHHHHHCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 6Y

Specific reaction: 2 S-adenosyl-L-methionine + precorrin-6Y = 2 S-adenosyl-L-homocysteine + precorrin-8X + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]