Click here to switch to the map view.

The map label for this gene is cobI [H]

Identifier: 153934800

GI number: 153934800

Start: 1020379

End: 1021095

Strand: Direct

Name: cobI [H]

Synonym: CLC_1002

Alternate gene names: 153934800

Gene position: 1020379-1021095 (Clockwise)

Preceding gene: 153936120

Following gene: 153935265

Centisome position: 27.13

GC content: 28.73

Gene sequence:

>717_bases
ATGAAAGATATATATAATAATATAGAATTTGAAAATAGGGATTTAAATAAAAAATACGGAACCCTATATGGAATTGGGGT
AGGTCCAGGAAATGAAGAATTATTAACTATAAAAGCTGTAAAAATTTTAGAAAGCTGTGATGTGGTTATAGCTCCAACAG
CAAGAGAGAATGGGGAGAGCATAGCTATAAATACAGCTAAGAATTTTATAAACCCTAAAGCAGAAATATATCTAAAATAT
TTTCCTATGAAAAAGGAAAAGGAAGCAGAGATATATGAAAACTATAGATTTATGAAAAAGTTATTATCAGAAGGTAAAAA
TGTAGTATTTCTAACTATAGGAGACCCTTTTGTATATAGCACTTATATATATTTATTACAATACATGAAAAATCATAATT
TAAATATAGAAACAGTACCAGGTATAACTTCTTTCTGTGCAGCAGCAAGTTTGGCGGGGGAAACTTTAGTTATAGGAGAT
GAACCACTTTTAATATTGCCAGGGAATAGGTTAGATTCCATAAAGGATGAAAAATACTTAGTAATAATGAAATACTACAA
AAATACAGAAGAAGTTTTGGACAAGCTAGAAGAAGAAGGTTTTAAATATGTATGCGTAAAAAGAGCTTATAGGGAAGGAC
AAGAAATATTAAGAACAAGAGAAGAAATCTTAAATTCTAAAGATTATATGTCAATTATAATAGCCAATAGGGAATAG

Upstream 100 bases:

>100_bases
AGGTGGATTAAGAGTTTAAGACACACTTTTTATAGATGATAAATACTTAAAATTAATTTATATAAAGAAATATAAATCAT
TGATATTAAGGAGTTAACAC

Downstream 100 bases:

>100_bases
AAAAATTGGAGGTGCAATGTGGAAAGTAAGGTTTATTTTATAGGAGCAGGTCCTGGGAACCCGGATTTAATAACAGTAAA
GGGAAGAGATATTTTAACTA

Product: cobalt-precorrin-2 C(20)-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKDIYNNIEFENRDLNKKYGTLYGIGVGPGNEELLTIKAVKILESCDVVIAPTARENGESIAINTAKNFINPKAEIYLKY
FPMKKEKEAEIYENYRFMKKLLSEGKNVVFLTIGDPFVYSTYIYLLQYMKNHNLNIETVPGITSFCAAASLAGETLVIGD
EPLLILPGNRLDSIKDEKYLVIMKYYKNTEEVLDKLEEEGFKYVCVKRAYREGQEILRTREEILNSKDYMSIIIANRE

Sequences:

>Translated_238_residues
MKDIYNNIEFENRDLNKKYGTLYGIGVGPGNEELLTIKAVKILESCDVVIAPTARENGESIAINTAKNFINPKAEIYLKY
FPMKKEKEAEIYENYRFMKKLLSEGKNVVFLTIGDPFVYSTYIYLLQYMKNHNLNIETVPGITSFCAAASLAGETLVIGD
EPLLILPGNRLDSIKDEKYLVIMKYYKNTEEVLDKLEEEGFKYVCVKRAYREGQEILRTREEILNSKDYMSIIIANRE
>Mature_238_residues
MKDIYNNIEFENRDLNKKYGTLYGIGVGPGNEELLTIKAVKILESCDVVIAPTARENGESIAINTAKNFINPKAEIYLKY
FPMKKEKEAEIYENYRFMKKLLSEGKNVVFLTIGDPFVYSTYIYLLQYMKNHNLNIETVPGITSFCAAASLAGETLVIGD
EPLLILPGNRLDSIKDEKYLVIMKYYKNTEEVLDKLEEEGFKYVCVKRAYREGQEILRTREEILNSKDYMSIIIANRE

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 27392; Mature: 27392

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIYNNIEFENRDLNKKYGTLYGIGVGPGNEELLTIKAVKILESCDVVIAPTARENGES
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCEEEECCCCCCCCE
IAINTAKNFINPKAEIYLKYFPMKKEKEAEIYENYRFMKKLLSEGKNVVFLTIGDPFVYS
EEEECHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
TYIYLLQYMKNHNLNIETVPGITSFCAAASLAGETLVIGDEPLLILPGNRLDSIKDEKYL
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCEE
VIMKYYKNTEEVLDKLEEEGFKYVCVKRAYREGQEILRTREEILNSKDYMSIIIANRE
EEEHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
>Mature Secondary Structure
MKDIYNNIEFENRDLNKKYGTLYGIGVGPGNEELLTIKAVKILESCDVVIAPTARENGES
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCEEEECCCCCCCCE
IAINTAKNFINPKAEIYLKYFPMKKEKEAEIYENYRFMKKLLSEGKNVVFLTIGDPFVYS
EEEECHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
TYIYLLQYMKNHNLNIETVPGITSFCAAASLAGETLVIGDEPLLILPGNRLDSIKDEKYL
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCCCCCEE
VIMKYYKNTEEVLDKLEEEGFKYVCVKRAYREGQEILRTREEILNSKDYMSIIIANRE
EEEHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]