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The map label for this gene is pdhD [H]

Identifier: 153934617

GI number: 153934617

Start: 778818

End: 780206

Strand: Direct

Name: pdhD [H]

Synonym: CLC_0754

Alternate gene names: 153934617

Gene position: 778818-780206 (Clockwise)

Preceding gene: 153937289

Following gene: 153935281

Centisome position: 20.71

GC content: 31.68

Gene sequence:

>1389_bases
ATGAAGGATATTATAGTTATAGGTGGAGGTCCTGGTGGATATGTAGCGGCCATAAGGGCTGCACATTTAGGTGCTGATGT
AGCAGTTGTCGAGATGGATAGCTTTGGTGGAACATGCCTTAATAAAGGCTGTATACCAACAAAGACTTTATATAGAACAG
CAGAAATGATGAATATATTAAAACATATAGAGGACTTTGGAATAGAGGCTGAAAACTATAATCTAAATGTAGAGAAGGTT
CAGGAAAGAAAAAATAATGTAATAAAAGAATTAGTAGGTGGAGTTGAGAAATTACTAAAAGGAAATAATGTTGAAATTAT
AAAAGGTAAGGCTTTCTTAAAAGATAAAAATACTGTTTTAGTAGAAACGAAAGATGGGCAAGTTACATTAGAAGGAAAAA
ACATAATTATAGCAACGGGCTCAAATGCTGAAATGCCAGATATAAAAGGTATAAAGAATAAAAACATAATAATAAGTGAC
GATATTTTAGAATTTGATAGAATTCCTAGACATTTGGTAGTTTCAGGTGGCGGTGTTGTTGGAATGGAATTTGCTAATAT
CTTTAAAGCTATGGGAAGTGAAGTAACAGTAATAGTCGCTAGGGATTCTATATTATATGATATTGATAGAGAAATAAGTA
AAAGGTATAAAGTTATAGCGAAAAAGTCAGGAATAAATATATTAACATCAACTAAAATATTGGAGTTTGCTCAGGATGAT
AATAATGTAACTATAAAATGTGAAAGCAAAAAAGGTGAGTTTGAGTTAAATTCTGATATGGTTCTTATGGCTAAAGGTAG
AAGAGGAAACTTTACAGGTATGAACTTAGAAGAACTAGGGATCGAACATGATAAAAAGAAAATAATTGTAGATGATAATT
ATAAAACTAATATAGATGGAATATACGCAATTGGAGATGTAAATGGAATTTGTCTTTTAGCTCATGCAGCATCACATCAA
GGAATAGAGGTTGTAGAGCATATAATGGAAAATAAAGAATGCCATAAATCTGTGATTCCAAACTGTATATTTACATTCCC
TGAAATAGCTACTGTAGGCATGACGGAGGAAGAAATTAAGGAAAAAGGTATAGAGTATATAAAAAATAAATTCCTATTTG
GAGCAAATGGAAAAGCCTTGGCATTAGGTGAAGGTGAAGGTGTTGTAAAAGTTATATGTGAAAAGGAAAGTAAAAAGATT
TTAGGTATTCATATAATGGGTCCACATGCTTCAGATTTAATACATGAAGGTGTGGTAGCAATTGAAAAGGGTATGACAGT
AAATGATTTTAAAGAAGTAGTACATGCTCACCCAACATTAGGTGAAGCATTCTATGAAGCAATAATGGGATTAAATAAGG
AGGCCATTCACAGTATAAACAAAAAATAA

Upstream 100 bases:

>100_bases
GTTCAGAAGAGTTAAGATTTAAGAAAAGTAAAAATACAATTCTTATTTCTTAGCTCTTAAATTTTAAAATTAATTAGATT
ATAGGGGGAATAGTATTATT

Downstream 100 bases:

>100_bases
AAACGGGGGAAAAAATTATGAAATATATTGATAATTCAAATATCGATCCATATTTTAATTTAGCAGCTGAAGAGTACTTT
TTAAGACATAAAGATGATGA

Product: glycine cleavage system, dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEMDSFGGTCLNKGCIPTKTLYRTAEMMNILKHIEDFGIEAENYNLNVEKV
QERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVLVETKDGQVTLEGKNIIIATGSNAEMPDIKGIKNKNIIISD
DILEFDRIPRHLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAQDD
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIKEKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK

Sequences:

>Translated_462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEMDSFGGTCLNKGCIPTKTLYRTAEMMNILKHIEDFGIEAENYNLNVEKV
QERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVLVETKDGQVTLEGKNIIIATGSNAEMPDIKGIKNKNIIISD
DILEFDRIPRHLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAQDD
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIKEKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK
>Mature_462_residues
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEMDSFGGTCLNKGCIPTKTLYRTAEMMNILKHIEDFGIEAENYNLNVEKV
QERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVLVETKDGQVTLEGKNIIIATGSNAEMPDIKGIKNKNIIISD
DILEFDRIPRHLVVSGGGVVGMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAQDD
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDGIYAIGDVNGICLLAHAASHQ
GIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIKEKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKI
LGIHIMGPHASDLIHEGVVAIEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=38.8646288209607, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=31.5673289183223, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=28.4140969162996, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI291045266, Length=455, Percent_Identity=25.9340659340659, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=25.219298245614, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=25.219298245614, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=25.219298245614, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=25.219298245614, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=25.219298245614, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI291045268, Length=454, Percent_Identity=24.6696035242291, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786307, Length=446, Percent_Identity=35.8744394618834, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=31.6916488222698, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=30.4147465437788, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081717, Length=448, Percent_Identity=27.9017857142857, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=38.3771929824561, Blast_Score=301, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=29.2631578947368, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI71983429, Length=451, Percent_Identity=28.3813747228381, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71983419, Length=451, Percent_Identity=28.3813747228381, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=483, Percent_Identity=26.2939958592132, Blast_Score=111, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=38.0753138075314, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=29.8507462686567, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6325166, Length=458, Percent_Identity=29.0393013100437, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=38.9978213507625, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=29.8319327731092, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24640549, Length=476, Percent_Identity=29.8319327731092, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24640551, Length=476, Percent_Identity=29.8319327731092, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=28.39248434238, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50588; Mature: 50588

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEMDSFGGTCLNKGCIPTKTLYRTAEMMNIL
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
KHIEDFGIEAENYNLNVEKVQERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCEEE
VETKDGQVTLEGKNIIIATGSNAEMPDIKGIKNKNIIISDDILEFDRIPRHLVVSGGGVV
EEECCCEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHCCCCEEEEECCCEE
GMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAQDD
HHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHEECCCCEEEEHHHEEEEECCC
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDG
CCEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCE
IYAIGDVNGICLLAHAASHQGIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIK
EEEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCHHHCCCCCCEECCCCHHHHH
EKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKILGIHIMGPHASDLIHEGVVA
HHHHHHHHHCEEECCCCCEEEEECCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHCHHH
IEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MKDIIVIGGGPGGYVAAIRAAHLGADVAVVEMDSFGGTCLNKGCIPTKTLYRTAEMMNIL
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
KHIEDFGIEAENYNLNVEKVQERKNNVIKELVGGVEKLLKGNNVEIIKGKAFLKDKNTVL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCEEE
VETKDGQVTLEGKNIIIATGSNAEMPDIKGIKNKNIIISDDILEFDRIPRHLVVSGGGVV
EEECCCEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHCCCCEEEEECCCEE
GMEFANIFKAMGSEVTVIVARDSILYDIDREISKRYKVIAKKSGINILTSTKILEFAQDD
HHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHEECCCCEEEEHHHEEEEECCC
NNVTIKCESKKGEFELNSDMVLMAKGRRGNFTGMNLEELGIEHDKKKIIVDDNYKTNIDG
CCEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCE
IYAIGDVNGICLLAHAASHQGIEVVEHIMENKECHKSVIPNCIFTFPEIATVGMTEEEIK
EEEEECCCCEEEEEECCCCCCHHHHHHHHCCHHHHHHHCCHHHCCCCCCEECCCCHHHHH
EKGIEYIKNKFLFGANGKALALGEGEGVVKVICEKESKKILGIHIMGPHASDLIHEGVVA
HHHHHHHHHCEEECCCCCEEEEECCCCEEEEEEECCCCEEEEEEEECCCHHHHHHHCHHH
IEKGMTVNDFKEVVHAHPTLGEAFYEAIMGLNKEAIHSINKK
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]