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The map label for this gene is lplA [H]

Identifier: 153935281

GI number: 153935281

Start: 780224

End: 781201

Strand: Direct

Name: lplA [H]

Synonym: CLC_0755

Alternate gene names: 153935281

Gene position: 780224-781201 (Clockwise)

Preceding gene: 153934617

Following gene: 153936105

Centisome position: 20.75

GC content: 27.81

Gene sequence:

>978_bases
ATGAAATATATTGATAATTCAAATATCGATCCATATTTTAATTTAGCAGCTGAAGAGTACTTTTTAAGACATAAAGATGA
TGAATACTTTATTTTATGGAGAGATGAGCCTTGTGTTGTAGTTGGAAAGAATCAAAATACATTATCAGAAATAGATATGG
ATTATATAGAAACTAACAAGGTTAAGGTAGTAAGAAGACAGACAGGCGGTGGTGCTGTATTTCATGATTTAGGAAACTTA
AACTATACTTTTATAGTTAAAGACGATGGGAAGAGCTTTAATGATTTTGAAAGATTTTGTAAACCTATAATAGGAGTTTT
GAAGACATTAGGGGTTAAAGCTGAATTCTCAGGACGAAATGATTTATTAATAGATGGGAAAAAAATTTCTGGAACAGCTC
AATGTAAGTATAAAAATAGAGTGATGCATCATGGAACCCTTTTGTTTTCATCGGATATAATAAACTTAAGTGGTGCCCTA
AAACCTAAAAAGATAAAATTTCAAGACAAAGCAGTTAAGTCAGTTGTAAGCAGAATAACTAATATTTCCGAACATTTAAA
TTCAGAAATTGATGTTCTAACATTTAAAAATAAGATATTTGATTATATTTTAAAAAGTGAAAAAGATGCAAAGGTTATGC
CATTAACTCAAGATGAAATAGATGAAATTGAAAAAATTAAAAAGAGTAAATATGAAACCTGGGCTTGGAACTTTGGAAGT
TCTCCTAAGTATGATTTTTACAATGAAGGAAAGTTTACAGGTGGAACTATAGAGTTAAATCTAAAAGTAGAAAAAGGTGT
AATTAAAGATATAAAGATATTTGGAGATTTCTTTGGTGTTAAAGATATAAAAGAAATAGAAGATTTATTAAAGGGTACTG
AACACAAAAAGGAAGAAATAAAGAATGTAATAAAAAATGTTAATGTAGATGAATATTTTGCTAGAATAACTGCGGAAGAA
TTTTTAAGCTTGTTTTAA

Upstream 100 bases:

>100_bases
CTCACCCAACATTAGGTGAAGCATTCTATGAAGCAATAATGGGATTAAATAAGGAGGCCATTCACAGTATAAACAAAAAA
TAAAAACGGGGGAAAAAATT

Downstream 100 bases:

>100_bases
TATAATTGGTCATTAGTAATAGGAGCATTTGTTTTTGATGGATATAAAGCCTATAAATACAAGAAAAATGAAATTTGTTG
TGACCGCCATACTTCATATA

Product: putative lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKYIDNSNIDPYFNLAAEEYFLRHKDDEYFILWRDEPCVVVGKNQNTLSEIDMDYIETNKVKVVRRQTGGGAVFHDLGNL
NYTFIVKDDGKSFNDFERFCKPIIGVLKTLGVKAEFSGRNDLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIINLSGAL
KPKKIKFQDKAVKSVVSRITNISEHLNSEIDVLTFKNKIFDYILKSEKDAKVMPLTQDEIDEIEKIKKSKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDIKEIEDLLKGTEHKKEEIKNVIKNVNVDEYFARITAEE
FLSLF

Sequences:

>Translated_325_residues
MKYIDNSNIDPYFNLAAEEYFLRHKDDEYFILWRDEPCVVVGKNQNTLSEIDMDYIETNKVKVVRRQTGGGAVFHDLGNL
NYTFIVKDDGKSFNDFERFCKPIIGVLKTLGVKAEFSGRNDLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIINLSGAL
KPKKIKFQDKAVKSVVSRITNISEHLNSEIDVLTFKNKIFDYILKSEKDAKVMPLTQDEIDEIEKIKKSKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDIKEIEDLLKGTEHKKEEIKNVIKNVNVDEYFARITAEE
FLSLF
>Mature_325_residues
MKYIDNSNIDPYFNLAAEEYFLRHKDDEYFILWRDEPCVVVGKNQNTLSEIDMDYIETNKVKVVRRQTGGGAVFHDLGNL
NYTFIVKDDGKSFNDFERFCKPIIGVLKTLGVKAEFSGRNDLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIINLSGAL
KPKKIKFQDKAVKSVVSRITNISEHLNSEIDVLTFKNKIFDYILKSEKDAKVMPLTQDEIDEIEKIKKSKYETWAWNFGS
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDIKEIEDLLKGTEHKKEEIKNVIKNVNVDEYFARITAEE
FLSLF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=245, Percent_Identity=30.6122448979592, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI21729884, Length=245, Percent_Identity=30.6122448979592, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI21729882, Length=245, Percent_Identity=30.6122448979592, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI21729880, Length=245, Percent_Identity=30.6122448979592, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI21729878, Length=245, Percent_Identity=30.6122448979592, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1790846, Length=320, Percent_Identity=31.875, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71981909, Length=254, Percent_Identity=29.1338582677165, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322415, Length=284, Percent_Identity=30.2816901408451, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573464, Length=292, Percent_Identity=28.4246575342466, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37456; Mature: 37456

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIDNSNIDPYFNLAAEEYFLRHKDDEYFILWRDEPCVVVGKNQNTLSEIDMDYIETNK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCHHHHCCCC
VKVVRRQTGGGAVFHDLGNLNYTFIVKDDGKSFNDFERFCKPIIGVLKTLGVKAEFSGRN
EEEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCC
DLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIINLSGALKPKKIKFQDKAVKSVVSRIT
CEEEECCCCCCEEEEEHHCCHHHCCEEEEECCEEECCCCCCCCEEEHHHHHHHHHHHHHH
NISEHLNSEIDVLTFKNKIFDYILKSEKDAKVMPLTQDEIDEIEKIKKSKYETWAWNFGS
HHHHHHCCCEEEEEHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCC
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDIKEIEDLLKGTEHKKEEI
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
KNVIKNVNVDEYFARITAEEFLSLF
HHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYIDNSNIDPYFNLAAEEYFLRHKDDEYFILWRDEPCVVVGKNQNTLSEIDMDYIETNK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCHHHHCCCC
VKVVRRQTGGGAVFHDLGNLNYTFIVKDDGKSFNDFERFCKPIIGVLKTLGVKAEFSGRN
EEEEEEECCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCC
DLLIDGKKISGTAQCKYKNRVMHHGTLLFSSDIINLSGALKPKKIKFQDKAVKSVVSRIT
CEEEECCCCCCEEEEEHHCCHHHCCEEEEECCEEECCCCCCCCEEEHHHHHHHHHHHHHH
NISEHLNSEIDVLTFKNKIFDYILKSEKDAKVMPLTQDEIDEIEKIKKSKYETWAWNFGS
HHHHHHCCCEEEEEHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCC
SPKYDFYNEGKFTGGTIELNLKVEKGVIKDIKIFGDFFGVKDIKEIEDLLKGTEHKKEEI
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
KNVIKNVNVDEYFARITAEEFLSLF
HHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]