Click here to switch to the map view.

The map label for this gene is vanS [H]

Identifier: 153933939

GI number: 153933939

Start: 3555142

End: 3556488

Strand: Reverse

Name: vanS [H]

Synonym: CLB_3406

Alternate gene names: 153933939

Gene position: 3556488-3555142 (Counterclockwise)

Preceding gene: 153931133

Following gene: 153933072

Centisome position: 92.05

GC content: 24.87

Gene sequence:

>1347_bases
ATGCTACTTTTTGTTATATCTTTAATAATTACAATTTTTGGAATGATGATGGTTTTAGGGAGTCTAAATATTTCATCGTA
TAATATTAATAAATTTTTATTCCCAGAACATTTTAAGGATAAGGTTATAATAGAAGAATATGAGAAAACTGTTGGAAGGC
GTGTAGATAGGGTCATAAAAGGAAATATAAATCAGCTTAAAGTACCACATAAAATGGATGAAAAGCTTAAAAAAGAGTTT
CCATATAATTTTAGAGGGAAAACTTTAGTTTATATTATTAATTCTAATGGGGAAATACTAAGTTCTAATAATGAAGAAAA
TATGTATACTATACGTAAATCTACAATAAAAGAAGGACTAGAGTTAAAAAGATATGATGATAATATTATTATAAATATTA
GAGAAACAAAAAAAATAAGTGATAACTTATATATAGTAATAAATAATGATTCTGTTATAGATGGAGATTTTATTATAATG
TGTACGGGTTTATTTGCATTTATGATTATTTTTGCCATACTTGCAAAGGGAAGGTTAAAGTATATCATATCTATTAAAAA
AGGAGTAAACAAGCTATATTCATCAGATTTTGAAGATAAAATACCATTAAAATATAATAATGAGCTTACATCTCTGGCAA
TTGCATTAAATGATATGGGAGGAAAAATAAAAGAAAATAAGGAAAATGAAAAAGAATTTTTATTAAATATATCCCATGAC
TTAAGGACTCCATTAACCTCTATATTAGGGTTTCTAAATCTTTTAAAGCAGAAGAAATATGATATAGAAGAGGAGAGGGA
AGGTTATATAAATACTATTGAAGAACAAAGTTTATATTTGAAGACTCTTATAGATGAATTTTTTCAATTTTCAACGCTAA
AATGGAAAAATGTTAAACTAAATAAAGAAAATATAAATCTTCAAGAGATTTTACGCCAAATAAGTGATGGATTTTATCCT
CAACTAAAAGAAAATAAAATTAGTGTGTCTATGAATTTTCCTGAAAAATCTTTATATAAAGAGGTTGATGTTGATAAGTT
TATGAGAGTATTAGAAAATGTAATATCTAATGCTATAAAATATAGCCATAAAGGAACAGAAATTAGTTTAAATCTATATG
AAAATAAAGATAAAATAGTAATGGAATTTTGGAATACTCCTATAGAAGCTCTAAAGGAAGATGAGTTAGATTTTTTATTT
AAAAGATTTTATAAAAAAGATTTATCAAGAGGTAATAAAGGAGCAGGACTGGGGCTTTCTATAGCTTTAGAAGCAGTAAA
GCTTCATGGTGGAGAGTTATATGCAGTAATGAAAAATGAAAGATTGGGAATTATAATAAAATTATAA

Upstream 100 bases:

>100_bases
CTAAGTGATACTGTAAAAAACTCAAAATACATTAAAACTGTTTGGGGAGTGGGTTATAAGATTGAAAAGGAAGCTTAGAA
TTGCATTAACAACAGAACTG

Downstream 100 bases:

>100_bases
TAGCATATATAGTATAGAAGGGTTTTCAGGAAAAGGGGACCTTTCTTTTTTATTTAAAAATCATAATAAAAAAATTAATA
AATTGTTAACAATGATAAAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIKGNINQLKVPHKMDEKLKKEF
PYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVINNDSVIDGDFIIM
CTGLFAFMIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENKENEKEFLLNISHD
LRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKLNKENINLQEILRQISDGFYP
QLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTPIEALKEDELDFLF
KRFYKKDLSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL

Sequences:

>Translated_448_residues
MLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIKGNINQLKVPHKMDEKLKKEF
PYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVINNDSVIDGDFIIM
CTGLFAFMIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENKENEKEFLLNISHD
LRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKLNKENINLQEILRQISDGFYP
QLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTPIEALKEDELDFLF
KRFYKKDLSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL
>Mature_448_residues
MLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIKGNINQLKVPHKMDEKLKKEF
PYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVINNDSVIDGDFIIM
CTGLFAFMIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENKENEKEFLLNISHD
LRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKLNKENINLQEILRQISDGFYP
QLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTPIEALKEDELDFLF
KRFYKKDLSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=265, Percent_Identity=25.2830188679245, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1786783, Length=291, Percent_Identity=27.1477663230241, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788713, Length=209, Percent_Identity=29.1866028708134, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=25.9414225941423, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=26.4069264069264, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994928, Length=206, Percent_Identity=26.2135922330097, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693157, Length=222, Percent_Identity=23.8738738738739, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788279, Length=284, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=276, Percent_Identity=22.8260869565217, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=27.6785714285714, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790551, Length=236, Percent_Identity=24.5762711864407, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082128, Length=279, Percent_Identity=24.0143369175627, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52116; Mature: 52116

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIK
CHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCHHCCCCHHHHHHHHHHHHHHHHHHH
GNINQLKVPHKMDEKLKKEFPYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGL
CCCCCEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCEEEEHHHHHHCCC
ELKRYDDNIIINIRETKKISDNLYIVINNDSVIDGDFIIMCTGLFAFMIIFAILAKGRLK
EEEECCCCEEEEEECCCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEE
YIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENKENEKEFLLNISHD
EEEEHHHHHHHHHCCCCCCCCCEEECCCHHEEEEEEECCCCCCCCCCCCCHHHEEECCHH
LRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKL
HHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECEEE
NKENINLQEILRQISDGFYPQLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIK
CCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHH
YSHKGTEISLNLYENKDKIVMEFWNTPIEALKEDELDFLFKRFYKKDLSRGNKGAGLGLS
CCCCCCEEEEEEECCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
IALEAVKLHGGELYAVMKNERLGIIIKL
HHHHHHHCCCCEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIK
CHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCHHCCCCHHHHHHHHHHHHHHHHHHH
GNINQLKVPHKMDEKLKKEFPYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGL
CCCCCEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCEEEEHHHHHHCCC
ELKRYDDNIIINIRETKKISDNLYIVINNDSVIDGDFIIMCTGLFAFMIIFAILAKGRLK
EEEECCCCEEEEEECCCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEE
YIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENKENEKEFLLNISHD
EEEEHHHHHHHHHCCCCCCCCCEEECCCHHEEEEEEECCCCCCCCCCCCCHHHEEECCHH
LRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKL
HHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECEEE
NKENINLQEILRQISDGFYPQLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIK
CCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHH
YSHKGTEISLNLYENKDKIVMEFWNTPIEALKEDELDFLFKRFYKKDLSRGNKGAGLGLS
CCCCCCEEEEEEECCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
IALEAVKLHGGELYAVMKNERLGIIIKL
HHHHHHHCCCCEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]