| Definition | Clostridium botulinum A str. ATCC 19397, complete genome. |
|---|---|
| Accession | NC_009697 |
| Length | 3,863,450 |
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The map label for this gene is npr-3 [H]
Identifier: 153933732
GI number: 153933732
Start: 1541399
End: 1543192
Strand: Reverse
Name: npr-3 [H]
Synonym: CLB_1468
Alternate gene names: 153933732
Gene position: 1543192-1541399 (Counterclockwise)
Preceding gene: 153932555
Following gene: 153931486
Centisome position: 39.94
GC content: 31.33
Gene sequence:
>1794_bases ATGAAAAGTAAAAGATTATTAGCTACAGTGTTAAGTGCTGTGATCACTTTTTCAGCAGTTTCAGCAGTTTCAGCTGCACC TGTAGGAAAAGAAAGTAAAAGTGACCCAAAGACCACAACAATATCTTGGGACAAGAGTGAACAAAATACTAAAAAAGCTG CTACAGACATAAAACAAAAGAAATTTAACAACGCTCAAGAAGTAACTAACTTCTTTGAGAAAAACATATCTAAATTTGGG GTAAAAAAAGGTACTCTTAAGAGTACTAAAACTTTAAAAGATGACAAAGGTAAAACTCACTATCATACAATTTATGAAGT AGAAGGAATACCTGTATACTATGGAAGAATTGTCTTTACTACTGAAAAAGACTCAACTATGGACTCTATAAATGGTAGAG TTGATACTGTTTTTGAAAATGGAAATTGGAAAAACAAAATCAAACTATCAAAAGAAGATGCCATAGCAAAAGCTAAAACT GATATTAAAGATCAAAAATTCAACAAGGAAAAGACTGAGTTATATCTGTATAATTTCGAGGGCAAACCTTATGTAGTTTA TTTAGTAAACTTAATTACAAATAGCGGCAATTGGGATGTTTTTGTTAATGCTGAAGATGGCTCTATAGTAAATAAATTTA ATAATACTCCTACTTTAATTGACAATAAAGCTGAAAAATTACCTAATGCTAAAGAAATTAAAGATGGAGCTAAAAAAAAT GAAGCTAAAAAGGCTAATAAGGCTACCAATGTGAATAGTATAGTTAATGTACAAGGTCAAAGCGTTAAAGGAGTAGGAAG AACTAGCTTAGATGGACTAGTAAATATTGATCTAACTTATGGCTATGGAAGATATTATTTAAAAGATAATAATAGAAAAA TTTATATATACGATTTAAAAAATCAAGTTAATGACACTGACTTAGAAAATTATTTGACTTGGTACCCTGGGAACCACAAT GAAGCGCTAATGGGTAGATCTGAATTAGTGTCTAATTCCAATAATAATTTTGTAGATAATGATAAAGTTAACTCTGTAGA TGCTTATGTAAATATGGGTAAGTCTTATGATTATTATAAAAATAAACTATCTAGAAATAGTATTGATAATAAAGGTATGG ATGTTAAGGGATTTGTTCATGTAGGCAAAGATTATGGTAACGCCTTTTGGTATGGAGAATACGATAGTATGTTCTTTGGC GATGGTAACGGACTATACTTCTCCCCTCTTGCTAAAGCTTTAGATGTTGTAGGCCACGAACTAAGTCATGGTGTTACTAA TAAGCAATCCGATCTTAAATATGAAAAGGAATCTGGTGCTTTAAACGAATCCTTCTCAGATATTATGGGAACAGCTATTG AGGGTAAAAACTTTGAAATAGGTGAAGACTGCTGGATACCTAGTGATAGATATGGAGAGATAATGAGAGATATGAAGGAT CCATCTAGAGGAAATCAACCAGCTCATATGAAAGATTTTAGAGATCTTCCTGTTGATGAAGATCATGATTGGGGTGGGGT TCATACAAATTCAGGTATAATAAACCATGCTGCTTATTTAATTGCAGATGGATTTGAAAAAATGGGTGAAAAAGATAGTA AAGATATTATGGCAAAAATATTTTATATAGCTAACTGCTATTACTGGGATCAAATAACAGATTTTTCCAAATGCAGAAAT GATGTAGTTAAAGTTGCTAAAGATCTTTACGGTGACAATAGTAAAGAAGTTCAAATTGTAAAAAATGCTTTTGACCAAGT TGGAATAACTGCTACACCTCAATTGCCATTATAA
Upstream 100 bases:
>100_bases TATAAAATATATTTATAAAACTATATAATTTTAGTATTTTACTTTATAAACATTAAAAAGTTTATAAGTAAAAATATATT AAAATGGGGGTTATTTATTC
Downstream 100 bases:
>100_bases TCAAATATACTTTGATTATAAAATATATTTATAAAACTATATAATTTTAGCATTTTACTTTATAAACATTAATAGGTTTA TAAGTAAAAATATATTAAAT
Product: thermolysin metallopeptidase
Products: NA
Alternate protein names: Neutral protease [H]
Number of amino acids: Translated: 597; Mature: 597
Protein sequence:
>597_residues MKSKRLLATVLSAVITFSAVSAVSAAPVGKESKSDPKTTTISWDKSEQNTKKAATDIKQKKFNNAQEVTNFFEKNISKFG VKKGTLKSTKTLKDDKGKTHYHTIYEVEGIPVYYGRIVFTTEKDSTMDSINGRVDTVFENGNWKNKIKLSKEDAIAKAKT DIKDQKFNKEKTELYLYNFEGKPYVVYLVNLITNSGNWDVFVNAEDGSIVNKFNNTPTLIDNKAEKLPNAKEIKDGAKKN EAKKANKATNVNSIVNVQGQSVKGVGRTSLDGLVNIDLTYGYGRYYLKDNNRKIYIYDLKNQVNDTDLENYLTWYPGNHN EALMGRSELVSNSNNNFVDNDKVNSVDAYVNMGKSYDYYKNKLSRNSIDNKGMDVKGFVHVGKDYGNAFWYGEYDSMFFG DGNGLYFSPLAKALDVVGHELSHGVTNKQSDLKYEKESGALNESFSDIMGTAIEGKNFEIGEDCWIPSDRYGEIMRDMKD PSRGNQPAHMKDFRDLPVDEDHDWGGVHTNSGIINHAAYLIADGFEKMGEKDSKDIMAKIFYIANCYYWDQITDFSKCRN DVVKVAKDLYGDNSKEVQIVKNAFDQVGITATPQLPL
Sequences:
>Translated_597_residues MKSKRLLATVLSAVITFSAVSAVSAAPVGKESKSDPKTTTISWDKSEQNTKKAATDIKQKKFNNAQEVTNFFEKNISKFG VKKGTLKSTKTLKDDKGKTHYHTIYEVEGIPVYYGRIVFTTEKDSTMDSINGRVDTVFENGNWKNKIKLSKEDAIAKAKT DIKDQKFNKEKTELYLYNFEGKPYVVYLVNLITNSGNWDVFVNAEDGSIVNKFNNTPTLIDNKAEKLPNAKEIKDGAKKN EAKKANKATNVNSIVNVQGQSVKGVGRTSLDGLVNIDLTYGYGRYYLKDNNRKIYIYDLKNQVNDTDLENYLTWYPGNHN EALMGRSELVSNSNNNFVDNDKVNSVDAYVNMGKSYDYYKNKLSRNSIDNKGMDVKGFVHVGKDYGNAFWYGEYDSMFFG DGNGLYFSPLAKALDVVGHELSHGVTNKQSDLKYEKESGALNESFSDIMGTAIEGKNFEIGEDCWIPSDRYGEIMRDMKD PSRGNQPAHMKDFRDLPVDEDHDWGGVHTNSGIINHAAYLIADGFEKMGEKDSKDIMAKIFYIANCYYWDQITDFSKCRN DVVKVAKDLYGDNSKEVQIVKNAFDQVGITATPQLPL >Mature_597_residues MKSKRLLATVLSAVITFSAVSAVSAAPVGKESKSDPKTTTISWDKSEQNTKKAATDIKQKKFNNAQEVTNFFEKNISKFG VKKGTLKSTKTLKDDKGKTHYHTIYEVEGIPVYYGRIVFTTEKDSTMDSINGRVDTVFENGNWKNKIKLSKEDAIAKAKT DIKDQKFNKEKTELYLYNFEGKPYVVYLVNLITNSGNWDVFVNAEDGSIVNKFNNTPTLIDNKAEKLPNAKEIKDGAKKN EAKKANKATNVNSIVNVQGQSVKGVGRTSLDGLVNIDLTYGYGRYYLKDNNRKIYIYDLKNQVNDTDLENYLTWYPGNHN EALMGRSELVSNSNNNFVDNDKVNSVDAYVNMGKSYDYYKNKLSRNSIDNKGMDVKGFVHVGKDYGNAFWYGEYDSMFFG DGNGLYFSPLAKALDVVGHELSHGVTNKQSDLKYEKESGALNESFSDIMGTAIEGKNFEIGEDCWIPSDRYGEIMRDMKD PSRGNQPAHMKDFRDLPVDEDHDWGGVHTNSGIINHAAYLIADGFEKMGEKDSKDIMAKIFYIANCYYWDQITDFSKCRN DVVKVAKDLYGDNSKEVQIVKNAFDQVGITATPQLPL
Specific function: Extracellular zinc metalloprotease [H]
COG id: COG3227
COG function: function code E; Zinc metalloprotease (elastase)
Gene ontology:
Cell location: Secreted [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the peptidase M4 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005075 - InterPro: IPR013856 - InterPro: IPR001570 - InterPro: IPR011096 [H]
Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]
EC number: =3.4.24.28 [H]
Molecular weight: Translated: 67205; Mature: 67205
Theoretical pI: Translated: 7.33; Mature: 7.33
Prosite motif: PS00142 ZINC_PROTEASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKSKRLLATVLSAVITFSAVSAVSAAPVGKESKSDPKTTTISWDKSEQNTKKAATDIKQK CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH KFNNAQEVTNFFEKNISKFGVKKGTLKSTKTLKDDKGKTHYHTIYEVEGIPVYYGRIVFT HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEEE TEKDSTMDSINGRVDTVFENGNWKNKIKLSKEDAIAKAKTDIKDQKFNKEKTELYLYNFE ECCCCCHHHCCCEEEEEEECCCCCCEEEECHHHHHHHHHCCCCHHHCCCCCEEEEEEEEC GKPYVVYLVNLITNSGNWDVFVNAEDGSIVNKFNNTPTLIDNKAEKLPNAKEIKDGAKKN CCEEEEEEEEEHCCCCCEEEEEECCCCCHHHCCCCCCCEECCHHHHCCCHHHHHHHHHHH EAKKANKATNVNSIVNVQGQSVKGVGRTSLDGLVNIDLTYGYGRYYLKDNNRKIYIYDLK HHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEECCCCEEEEEECC NQVNDTDLENYLTWYPGNHNEALMGRSELVSNSNNNFVDNDKVNSVDAYVNMGKSYDYYK CCCCCCCCCCCEEECCCCCCCHHCCHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHH NKLSRNSIDNKGMDVKGFVHVGKDYGNAFWYGEYDSMFFGDGNGLYFSPLAKALDVVGHE HHHHHCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEECCCCEEHHHHHHHHHHHHHH LSHGVTNKQSDLKYEKESGALNESFSDIMGTAIEGKNFEIGEDCWIPSDRYGEIMRDMKD HHCCCCCCHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC PSRGNQPAHMKDFRDLPVDEDHDWGGVHTNSGIINHAAYLIADGFEKMGEKDSKDIMAKI CCCCCCCCHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH FYIANCYYWDQITDFSKCRNDVVKVAKDLYGDNSKEVQIVKNAFDQVGITATPQLPL HHHHHEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC >Mature Secondary Structure MKSKRLLATVLSAVITFSAVSAVSAAPVGKESKSDPKTTTISWDKSEQNTKKAATDIKQK CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH KFNNAQEVTNFFEKNISKFGVKKGTLKSTKTLKDDKGKTHYHTIYEVEGIPVYYGRIVFT HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEEE TEKDSTMDSINGRVDTVFENGNWKNKIKLSKEDAIAKAKTDIKDQKFNKEKTELYLYNFE ECCCCCHHHCCCEEEEEEECCCCCCEEEECHHHHHHHHHCCCCHHHCCCCCEEEEEEEEC GKPYVVYLVNLITNSGNWDVFVNAEDGSIVNKFNNTPTLIDNKAEKLPNAKEIKDGAKKN CCEEEEEEEEEHCCCCCEEEEEECCCCCHHHCCCCCCCEECCHHHHCCCHHHHHHHHHHH EAKKANKATNVNSIVNVQGQSVKGVGRTSLDGLVNIDLTYGYGRYYLKDNNRKIYIYDLK HHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCEEEEECCCCEEEEEECC NQVNDTDLENYLTWYPGNHNEALMGRSELVSNSNNNFVDNDKVNSVDAYVNMGKSYDYYK CCCCCCCCCCCEEECCCCCCCHHCCHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHH NKLSRNSIDNKGMDVKGFVHVGKDYGNAFWYGEYDSMFFGDGNGLYFSPLAKALDVVGHE HHHHHCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEECCCCEEHHHHHHHHHHHHHH LSHGVTNKQSDLKYEKESGALNESFSDIMGTAIEGKNFEIGEDCWIPSDRYGEIMRDMKD HHCCCCCCHHCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC PSRGNQPAHMKDFRDLPVDEDHDWGGVHTNSGIINHAAYLIADGFEKMGEKDSKDIMAKI CCCCCCCCHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH FYIANCYYWDQITDFSKCRNDVVKVAKDLYGDNSKEVQIVKNAFDQVGITATPQLPL HHHHHEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7764969 [H]