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The map label for this gene is glcA [H]

Identifier: 152977432

GI number: 152977432

Start: 3820740

End: 3822401

Strand: Reverse

Name: glcA [H]

Synonym: Bcer98_3763

Alternate gene names: 152977432

Gene position: 3822401-3820740 (Counterclockwise)

Preceding gene: 152977433

Following gene: 152977431

Centisome position: 93.53

GC content: 38.57

Gene sequence:

>1662_bases
ATGAACACATGGACACAAGTTTATGATCCGTTCGGGAACATTTGGATTTCCGCAGCGGTGGCACTTATTCCAATTATCTT
TTTTTTCTTGGCTTTAGCTGTTTTTCGTATGAAGGGGTACGTAGCAGGATTTATTACCGTTGTATTAACAATTTTGGTAG
CGCTATTTGTATATAAAATGCCATTTACAATGGCGATGGCGGCAACTGGATATGGTTTTCTATATGGGCTATGGCCAATT
GCTTGGATTATTATCATGTCGGTATTTTTGTATAAAATTTCTGTGAAAACAGGACAGTTTGATATTATTCGTGCATCTGT
ATTATCCATTACAAATGATCATCGTTTACTTGTCATTTTAATCGGTTTTTCATTTGGAGCATTTTTGGAAGGAGCAGCAG
GATTTGGAGCACCAGTAGCCATCACAGCAGCGCTTCTAGCAGGACTTGGCTTAAATCCTTTATATGCAGCAGGGCTTTGC
TTAATTGCAAATACAGCGCCAGTAGCATTTGGAGCAATGGGAATACCGATTACAGTTGCAGGGCAAGTAACAGGTATTGA
TCCTCATAAAATTGGACAAATGGCTGGGCACCAATTGCCATTCTTATCGTTATTTGTTCCGTTCTTTATTGTATTTTTAA
TGGATGGTTTCAAGGGAATAAAGCAAACATGGCCGGCGTTATTTGTAGCGGGGGGTTCTTTTGCAATTACACAATTTATT
ACGGCGACATTTTTAGGACCAGAACTTCCAGATATTACATCAGCGCTTGTCAGCTTAGTATCTTTATCGCTATTTTTAAA
AGTATGGCAGCCAAAAGAAGTGTATCAATCGAATCAAGCAAATGTGGAAGCTGCAGCAACAGCGGAAGCTTCATCGATGC
CAAATTTAACAGGTAAAAAAGTTGTGAAAGCTTGGTCGCCATTTATTATTTTGACTGCTATGGTAGTGATTTGGAGTCAA
GATTTCTTTAAAGCATTATTTGCTCCAGGTGGGGCGTTAGAAAGTTTTGTCATGAAGTTTGAAATTCCGGGTCTGCACAA
TTTAGTCATGAAGGCAGAACCAATTGTAAATAAACCAACGCCTTATGAGGCTGTATTAAAATTTGATATTGTATCGGCAA
CCGGAACAGCTATTTTAATCGCATGTATCATTTCCATTTTTGTATTAAAAATGAGTGCAAAACAAGCGGTTGAAACATTG
AAAGAAACATTACAAGAATTAAAAATGCCAATTTTATCTATTGGATTTGTATTAGGCTTTGCATTTATTGCGAATTATTC
TGGTCTATCATCTACACTTGCACTAGCGTTAGCTGGAACAGGAGGATTATTCCCGTTCTTCTCGCCATTTCTTGGCTGGA
TCGGCGTATTCTTAACAGGATCAGATACATCGGCAAATGCGCTATTCTCGAACTTACAATCGATTACAGCGCAGCAAGTA
GGTGTAAAAGAAGTATTACTTGTTGCGGCAAATACGACAGGTGGTGTAACGGGGAAAATGATTTCTCCGCAATCGATTGC
AATCGCGTGCGCAGCAGTAGGACTTGCAGGAAAAGAATCCGATTTATTCCGCTTTACAGTAAAACATAGTTTGTTCTTTG
TCATTATCGTTGGAATTATGACGTATGTACAAGCTTATTATTTAACGTGGATGATACCGTAA

Upstream 100 bases:

>100_bases
CTTAAGTGTCTTATTTTTATCTTAAAGGTATGATGACCATACAGTATGCTGATAAGGTTATGGATATGTGAAAAAGGGAT
TATAGAAAGGGAGAGGGACA

Downstream 100 bases:

>100_bases
AAGTGTAAATTGATTTGCATTTTTCAGATTATAGTGTATGATAGAAAAGTATTAAAAATTAAATCAGTGATAAACTAGGG
GTGCCCAATGTATGCGTGGG

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFVYKMPFTMAMAATGYGFLYGLWPI
AWIIIMSVFLYKISVKTGQFDIIRASVLSITNDHRLLVILIGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLC
LIANTAPVAFGAMGIPITVAGQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGFKGIKQTWPALFVAGGSFAITQFI
TATFLGPELPDITSALVSLVSLSLFLKVWQPKEVYQSNQANVEAAATAEASSMPNLTGKKVVKAWSPFIILTAMVVIWSQ
DFFKALFAPGGALESFVMKFEIPGLHNLVMKAEPIVNKPTPYEAVLKFDIVSATGTAILIACIISIFVLKMSAKQAVETL
KETLQELKMPILSIGFVLGFAFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQSITAQQV
GVKEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMTYVQAYYLTWMIP

Sequences:

>Translated_553_residues
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFVYKMPFTMAMAATGYGFLYGLWPI
AWIIIMSVFLYKISVKTGQFDIIRASVLSITNDHRLLVILIGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLC
LIANTAPVAFGAMGIPITVAGQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGFKGIKQTWPALFVAGGSFAITQFI
TATFLGPELPDITSALVSLVSLSLFLKVWQPKEVYQSNQANVEAAATAEASSMPNLTGKKVVKAWSPFIILTAMVVIWSQ
DFFKALFAPGGALESFVMKFEIPGLHNLVMKAEPIVNKPTPYEAVLKFDIVSATGTAILIACIISIFVLKMSAKQAVETL
KETLQELKMPILSIGFVLGFAFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQSITAQQV
GVKEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMTYVQAYYLTWMIP
>Mature_553_residues
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFVYKMPFTMAMAATGYGFLYGLWPI
AWIIIMSVFLYKISVKTGQFDIIRASVLSITNDHRLLVILIGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLC
LIANTAPVAFGAMGIPITVAGQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGFKGIKQTWPALFVAGGSFAITQFI
TATFLGPELPDITSALVSLVSLSLFLKVWQPKEVYQSNQANVEAAATAEASSMPNLTGKKVVKAWSPFIILTAMVVIWSQ
DFFKALFAPGGALESFVMKFEIPGLHNLVMKAEPIVNKPTPYEAVLKFDIVSATGTAILIACIISIFVLKMSAKQAVETL
KETLQELKMPILSIGFVLGFAFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQSITAQQV
GVKEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIMTYVQAYYLTWMIP

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=558, Percent_Identity=66.4874551971326, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1790031, Length=558, Percent_Identity=64.8745519713262, Blast_Score=687, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 59227; Mature: 59227

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFVYKM
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFTMAMAATGYGFLYGLWPIAWIIIMSVFLYKISVKTGQFDIIRASVLSITNDHRLLVIL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEE
IGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLCLIANTAPVAFGAMGIPITVA
EECCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHCCCCCCEEEE
GQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGFKGIKQTWPALFVAGGSFAITQFI
CEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
TATFLGPELPDITSALVSLVSLSLFLKVWQPKEVYQSNQANVEAAATAEASSMPNLTGKK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHCCCCCCCCCCHHH
VVKAWSPFIILTAMVVIWSQDFFKALFAPGGALESFVMKFEIPGLHNLVMKAEPIVNKPT
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
PYEAVLKFDIVSATGTAILIACIISIFVLKMSAKQAVETLKETLQELKMPILSIGFVLGF
CHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQSITAQQV
HHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHC
GVKEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIM
CHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TYVQAYYLTWMIP
HHHHHHHHHEECC
>Mature Secondary Structure
MNTWTQVYDPFGNIWISAAVALIPIIFFFLALAVFRMKGYVAGFITVVLTILVALFVYKM
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFTMAMAATGYGFLYGLWPIAWIIIMSVFLYKISVKTGQFDIIRASVLSITNDHRLLVIL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEE
IGFSFGAFLEGAAGFGAPVAITAALLAGLGLNPLYAAGLCLIANTAPVAFGAMGIPITVA
EECCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHCCCCCCEEEE
GQVTGIDPHKIGQMAGHQLPFLSLFVPFFIVFLMDGFKGIKQTWPALFVAGGSFAITQFI
CEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHH
TATFLGPELPDITSALVSLVSLSLFLKVWQPKEVYQSNQANVEAAATAEASSMPNLTGKK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHCCCCCCCCCCHHH
VVKAWSPFIILTAMVVIWSQDFFKALFAPGGALESFVMKFEIPGLHNLVMKAEPIVNKPT
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCC
PYEAVLKFDIVSATGTAILIACIISIFVLKMSAKQAVETLKETLQELKMPILSIGFVLGF
CHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFIANYSGLSSTLALALAGTGGLFPFFSPFLGWIGVFLTGSDTSANALFSNLQSITAQQV
HHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHC
GVKEVLLVAANTTGGVTGKMISPQSIAIACAAVGLAGKESDLFRFTVKHSLFFVIIVGIM
CHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TYVQAYYLTWMIP
HHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]