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The map label for this gene is thiC [H]

Identifier: 152977431

GI number: 152977431

Start: 3818478

End: 3820238

Strand: Reverse

Name: thiC [H]

Synonym: Bcer98_3762

Alternate gene names: 152977431

Gene position: 3820238-3818478 (Counterclockwise)

Preceding gene: 152977432

Following gene: 152977428

Centisome position: 93.47

GC content: 41.96

Gene sequence:

>1761_bases
ATGGAACAATCTGTTTCAGCTGAGCAAATTGAATTAAAGTCGAGTTTACCAGGGAGTAAGAAAGTGTATGTGGAGGGGTC
ACGTGAAGGTATGAAAGTGCCGATGCGTGAGATTGAATTAAGTGACACAAACGGAGTGCCAAATTCCCCGATTCGTGTAT
ATGATACGAGCGGTCCCTATACGGATCCAGAATATAAAGTGGAACTCGAAAAAGGGATTCCAACTCCTCGTCGCAATTGG
ATTATAGAACGCGGAGATGTAGAGGAGTATGAGGGACGCGAAATCAAGCCGGAAGATGATGGTGTAAAAGCTGCTTCGAA
CCATACACCTGTGTTCCCGCAAATGGATCGTAAACCTCTTAGAGCAAAGAAAGGTGCGAATGTCACGCAGATGCATTATG
CTCGTAAAGGGATTATTACATCTGAGATGGAATATGTTGCAATTCGTGAAGGGGTAGAACCGGAGTTTGTTCGAAAAGAG
ATTGCCGAAGGACGGGCCATTTTACCAGCGAATATTAACCATCCAGAAGCAGAACCAATGATTATCGGCCGTAATTTCCA
TGTGAAAGTAAATGCGAATATCGGAAATTCTGCTGTTTCGTCTTCGATTGCCGAAGAAGTAGAAAAGATGACATGGGCAA
CGCGCTGGGGTGCGGATACAATTATGGATTTATCAACGGGGAAAAATATTCATACAACGCGCGAATGGATTATTCGTAAT
GCCCCTGTACCAGTTGGGACAGTGCCGATTTACCAAGCGTTAGAAAAGGTACAGGGGATTGCTGAAAACTTAACTTGGGA
AGTATACCGCGATACATTAATTGAACAAGCGGAACAAGGTGTGGATTATTTTACAATTCACGCGGGCGTATTATTGCGCT
ACATCCCGCTCACAGCAAAACGCATGACGGGAATTGTTTCGCGCGGCGGATCAATTATGGCGCAGTGGTGTCTATACCAT
CATAAAGAAAACTTCTTGTATACTCATTTTGAAGAAATTTGTGAGATTATGAAACAGTATGATGTTTCCTTCTCTTTAGG
AGATGGGTTACGCCCAGGATCTATTGCAGATGCAAATGATGAAGCTCAGTTTGCAGAGCTTGAAACGTTAGGGGAATTAA
CGAAAATTGCTTGGAAGCATGATGTGCAAGTCATGATTGAGGGGCCAGGGCACGTACCGATGCATTTAATTAAAGAAAAT
ATGGAGAAAGAAATAGATATTTGTCAAGGTGCGCCTTTCTATACGCTGGGGCCTTTAACAACAGATATCGCACCAGGTTA
TGATCATATTACTTCTGCAATTGGTGCAGCGATGATTGGATGGTTTGGGACTGCGATGCTTTGTTATGTAACACCGAAAG
AGCATTTAGGATTGCCAAATAAAGATGATGTTCGAGAAGGTGTGATTACTTACAAAATTGCAGCGCATGCAGCTGACCTT
GCAAAAGGACATAAAACAGCGCAACAAAGAGATGATGCGTTATCAAAAGCGCGCTTTGAATTCCGTTGGCGCGATCAATT
CAATCTATCGTTAGATCCTGAAAGAGCAATGGAATTTCATGATGAAACATTGCCAGCAGAAGGAGCGAAAACAGCTCATT
TCTGTTCGATGTGTGGCCCGAAATTTTGTAGTATGAAAATTTCACATGATATTCGGGAGTATGCAAAAGAAAATAATTTA
GAAACGACAGAAGCAATTGAAAAAGGAATGAAAGAAAAAGCAAAAGAATTTAAAGAGGCTGGTAGTCACTTATATCAATA
A

Upstream 100 bases:

>100_bases
CAAGAAATGCGGAGCTGACTGTTTAGCAGCTGAAGGGAGAATCGAATTTTTCTAGGTAAAGTAGATTTCTTGAAATGATA
AAGGGGAGGAAACAAAAATC

Downstream 100 bases:

>100_bases
TGACGTTCGATGTTTTTGAGAGTTGTTTATAGCAACCTTCTCATGTATGCAAGATAGGGTATGAATGTTCTAAAAATAGA
AATATGTACGATTTTGAACA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MEQSVSAEQIELKSSLPGSKKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPYTDPEYKVELEKGIPTPRRNW
IIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKGANVTQMHYARKGIITSEMEYVAIREGVEPEFVRKE
IAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
APVPVGTVPIYQALEKVQGIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIMAQWCLYH
HKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFAELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKEN
MEKEIDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
AKGHKTAQQRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSMKISHDIREYAKENNL
ETTEAIEKGMKEKAKEFKEAGSHLYQ

Sequences:

>Translated_586_residues
MEQSVSAEQIELKSSLPGSKKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPYTDPEYKVELEKGIPTPRRNW
IIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKGANVTQMHYARKGIITSEMEYVAIREGVEPEFVRKE
IAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
APVPVGTVPIYQALEKVQGIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIMAQWCLYH
HKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFAELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKEN
MEKEIDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
AKGHKTAQQRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSMKISHDIREYAKENNL
ETTEAIEKGMKEKAKEFKEAGSHLYQ
>Mature_586_residues
MEQSVSAEQIELKSSLPGSKKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPYTDPEYKVELEKGIPTPRRNW
IIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKGANVTQMHYARKGIITSEMEYVAIREGVEPEFVRKE
IAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
APVPVGTVPIYQALEKVQGIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIMAQWCLYH
HKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFAELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKEN
MEKEIDICQGAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
AKGHKTAQQRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSMKISHDIREYAKENNL
ETTEAIEKGMKEKAKEFKEAGSHLYQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=613, Percent_Identity=64.6003262642741, Blast_Score=817, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 65873; Mature: 65873

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSVSAEQIELKSSLPGSKKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPY
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHEEECCCCCCCCCCEEEEECCCCC
TDPEYKVELEKGIPTPRRNWIIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPL
CCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
RAKKGANVTQMHYARKGIITSEMEYVAIREGVEPEFVRKEIAEGRAILPANINHPEAEPM
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCCCE
IIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
EECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
APVPVGTVPIYQALEKVQGIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAK
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
RMTGIVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADAND
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
EAQFAELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKENMEKEIDICQGAPFYTLGPLT
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCC
TDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHH
AKGHKTAQQRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGP
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCC
KFCSMKISHDIREYAKENNLETTEAIEKGMKEKAKEFKEAGSHLYQ
HHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQSVSAEQIELKSSLPGSKKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPY
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHEEECCCCCCCCCCEEEEECCCCC
TDPEYKVELEKGIPTPRRNWIIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPL
CCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
RAKKGANVTQMHYARKGIITSEMEYVAIREGVEPEFVRKEIAEGRAILPANINHPEAEPM
HHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCCCE
IIGRNFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRN
EECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCC
APVPVGTVPIYQALEKVQGIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAK
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
RMTGIVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADAND
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
EAQFAELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKENMEKEIDICQGAPFYTLGPLT
CHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCC
TDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADL
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCCEEEEHHHHHHHH
AKGHKTAQQRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGP
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCC
KFCSMKISHDIREYAKENNLETTEAIEKGMKEKAKEFKEAGSHLYQ
HHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA