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The map label for this gene is pfkA [H]

Identifier: 152976980

GI number: 152976980

Start: 3353496

End: 3354455

Strand: Reverse

Name: pfkA [H]

Synonym: Bcer98_3283

Alternate gene names: 152976980

Gene position: 3354455-3353496 (Counterclockwise)

Preceding gene: 152976981

Following gene: 152976979

Centisome position: 82.08

GC content: 39.48

Gene sequence:

>960_bases
ATGAAACGTATTGGTGTATTAACGAGTGGTGGAGATTCACCTGGTATGAACGCTGCTATTCGTGCAGTTGTTCGTAAAGC
GATTTACCATGATATTGAAGTATATGGTATCTACCATGGATATGCTGGACTAATTTCTGGTCACATTGAAAAATTAGAAC
TTGGTTCTGTTGGCGATATTATCCACCGTGGTGGTACAAAATTATATACAGCAAGATGCCCTGAGTTTAAAGATCCAGCT
GTTCGTGAAAAAGGAATCGAACAATTAAAGAAACATGGCATTGAAGGACTTGTTGTTATTGGTGGAGACGGTTCTTACCA
AGGAGCTAAAAAACTAACAGAGCAAGGATTCCCTTGTGTAGGTGTACCAGGTACAATCGACAATGATATTCCTGGAACAG
ACTTTACAATTGGATTCGATACTGCTTTAAATACTGTTATTGATGCAATTGATAAAATTCGTGATACAGCTACATCTCAT
GAACGTACATACGTTATTGAAGTAATGGGGCGTCACGCTGGCGACATCGCATTATGGGCTGGTTTAGCTGATGGTGCAGA
GACAATCTTGATTCCAGAAGCAGAATATGATATGGATGATGTAATTGCTCGTTTAAAACGCGGTAGCGAACGTGGTAAAA
AACATAGTATCATCGTTGTAGCTGAAGGTGTTGGAAGTGCGATTGATATCGGTAAGAAAATTGAGGAAGCGACAAACTTT
GATACTCGTGTAACAGTATTAGGTCATGTACAACGTGGTGGATCACCAAGTGCACAAGACCGTGTGTTAGCGAGTCGCCT
TGGCGCAAGAGCAGTTGAATTATTAATGGAAGGTAAGGGCGGACGTTGTGTTGGTATCCAAAACAACAAACTCGTTGATC
ATGATATTATCGAAGCGTTAGCTCAAAAACATGTAATTGATATAGATATGTATCAATTATCTAAAGAATTATCCATCTAA

Upstream 100 bases:

>100_bases
CACGTTTTTATTGTGAAAAGACACATCTTCTCCATTGTATTTTTATATTTCTTCATGTTATTTTATTATAAGGCAAATAA
TCTTTATGAGGTGAGTACAA

Downstream 100 bases:

>100_bases
TCGACGTAATGTCGGATATTTGGGAACGGTTTCAGAAATTTCGGAGGTGCAATATGCGCAAAACAAAAATTGTATGTACT
ATAGGTCCTGCTAGTGAAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDIEVYGIYHGYAGLISGHIEKLELGSVGDIIHRGGTKLYTARCPEFKDPA
VREKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLADGAETILIPEAEYDMDDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKKIEEATNF
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLMEGKGGRCVGIQNNKLVDHDIIEALAQKHVIDIDMYQLSKELSI

Sequences:

>Translated_319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDIEVYGIYHGYAGLISGHIEKLELGSVGDIIHRGGTKLYTARCPEFKDPA
VREKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLADGAETILIPEAEYDMDDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKKIEEATNF
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLMEGKGGRCVGIQNNKLVDHDIIEALAQKHVIDIDMYQLSKELSI
>Mature_319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDIEVYGIYHGYAGLISGHIEKLELGSVGDIIHRGGTKLYTARCPEFKDPA
VREKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSH
ERTYVIEVMGRHAGDIALWAGLADGAETILIPEAEYDMDDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKKIEEATNF
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLMEGKGGRCVGIQNNKLVDHDIIEALAQKHVIDIDMYQLSKELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=315, Percent_Identity=45.0793650793651, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI48762920, Length=314, Percent_Identity=45.2229299363057, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=44.2307692307692, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=44.2307692307692, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=44.2307692307692, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=44.2307692307692, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=55.6603773584906, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25147584, Length=347, Percent_Identity=41.2103746397695, Blast_Score=224, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=39.1585760517799, Blast_Score=200, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=310, Percent_Identity=32.9032258064516, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6321679, Length=338, Percent_Identity=38.7573964497041, Blast_Score=205, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=343, Percent_Identity=37.3177842565598, Blast_Score=199, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24652337, Length=312, Percent_Identity=46.1538461538462, Blast_Score=237, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=46.1538461538462, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=45.1923076923077, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34260; Mature: 34260

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDIEVYGIYHGYAGLISGHIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHCCHHHEECCCHHHH
IHRGGTKLYTARCPEFKDPAVREKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCV
HHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLADGAETILIPEAEYDMDDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKKIEEATNF
ECCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLMEGKGGRCVGIQNNKLVDHDIIEAL
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHH
AQKHVIDIDMYQLSKELSI
HHHCEEEHHHHHHHHHCCC
>Mature Secondary Structure
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDIEVYGIYHGYAGLISGHIEKLELGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHCCHHHEECCCHHHH
IHRGGTKLYTARCPEFKDPAVREKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEQGFPCV
HHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEE
GLADGAETILIPEAEYDMDDVIARLKRGSERGKKHSIIVVAEGVGSAIDIGKKIEEATNF
ECCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
DTRVTVLGHVQRGGSPSAQDRVLASRLGARAVELLMEGKGGRCVGIQNNKLVDHDIIEAL
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHH
AQKHVIDIDMYQLSKELSI
HHHCEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA