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The map label for this gene is pyk [H]

Identifier: 152976979

GI number: 152976979

Start: 3351685

End: 3353442

Strand: Reverse

Name: pyk [H]

Synonym: Bcer98_3282

Alternate gene names: 152976979

Gene position: 3353442-3351685 (Counterclockwise)

Preceding gene: 152976980

Following gene: 152976978

Centisome position: 82.05

GC content: 39.02

Gene sequence:

>1758_bases
ATGCGCAAAACAAAAATTGTATGTACTATAGGTCCTGCTAGTGAAAGTATTGAAAAATTAGAACAGTTAATTGAGGCTGG
TATGAACGTTGCTCGTTTAAACTTCTCTCATGGTAGCCATGAAGAGCACGGAGCACGTATTAAAAACATTCGTGAAGCGT
CTAAAAAGACTGGTAAAACAGTTGCCATCTTGCTTGATACAAAAGGTCCAGAAATTCGTACACATGACTTCGTAGACGGA
CAAGCTGAGCTAACAACTGGTTCAGAAGTAGTTATTTCTACTGAGCAAGTATTAGGAACTGCAGAAAAGTTCTCAGTAAC
TTATGCTGGTTTATATGACGATGTAAATCCAGGTTCTCGCATTCTAATCGATGATGGTTTAATCGAGCTAGAAGTAATCG
AGAAAGTTGATGGAAATATTCGTACAAAAGTACTAAACAGTGGTATTGTAAAAAATAAAAAAGGTGTAAACGTACCAAAC
GTAAGCATTAAGCTTCCTGGTATTACTGAAAAAGACGTAAAAGATATCATCTTCGGTATCGAGCAAAACGTTGACTTCAT
CGCAGCATCTTTCGTTCGTAAAGCGGCTGACGTGTTAGAAATTCGTGAATTATTAGAAGAACATAATGCACAGCATATCC
AAATCGTACCAAAAATTGAAAACCAAGAGGGTATCGATAATATCGATTCAATCTTAGAAGTTTCTGATGGCTTAATGGTA
GCTCGTGGTGACATGGGTGTAGAAATTCCACCAGAAGAAGTGCCATTAGTACAAAAACGTCTAATCAAAAAATGTAACGT
ATTAGGCAAACCAGTTATTACTGCAACACAAATGTTAGATTCTATGCAACGTAACCCACGTCCAACTCGTGCGGAAGCAA
GTGACGTAGCAAATGCAATCTTTGACGGTACAGATGCAATCATGCTTTCAGGTGAAACTGCAGCAGGGCAATATCCAGTA
GAAGCAGTAACAATGATGGCGAATATTGCAACACGCGTTGAGAAGTCATTACAATATAAAGATATATTTAAGAAACGTAT
TAAAGAGCTTACACCAACAATTACAGATGCGATTAGTCAATCTGTTGCACATACAGCAATCGCTCTTGATGTAGCTGCAA
TCATTGCTCCAACTGAAAGTGGCTATACTGCAAAAATGATCTCGAAATACCGTCCAAAATCTCCAATCGTAGCTGTAACA
TCTGATGAGCAAGTATGTCGTCGTCTTGCACTTGTTTGGGGTGTACAAGCGTTTATGGCTAGTAAACGTGCGGCGTCTAC
TGATGAAATGTTAGATACTGCTATTCAAACAGGTATGGATGCTGGTCTAATTGGACTTGGTGATACTGTAGTTATTACAG
CTGGTGTTCCAGTTGCTGAAACGGGTACAACAAACTTAATGAAAATTCATGTTGTTGGCGAAGAAATCGCGAAAGGACAA
GGAATTGGTCGTAAAGCGGCAAAAGGTAAAGTGGTTGTAGCGAAAACAGCAACTGAAGCTGTTGCGAACGTAAAAGAAGG
AGATATTCTAGTTACAACAAGTACTGATAAAGATATGATTCCTGCAATTGAAAAAGCGGCAGCTTTAGTTGTAGAAGAAG
GCGGTTTAACAAGCCATGCAGCTGTCGTAGGTGTTTCAATTGGTATTCCAGTTATCGTTGGCGTAAATGGTGTAACAGAA
ACTTTAAAAAATGGCCAAGAAGTAACAGTTGATGCAGCTCGCGGAATTGTTTATAATGGACATGCGGAAGTGCTATAA

Upstream 100 bases:

>100_bases
TAATTGATATAGATATGTATCAATTATCTAAAGAATTATCCATCTAATCGACGTAATGTCGGATATTTGGGAACGGTTTC
AGAAATTTCGGAGGTGCAAT

Downstream 100 bases:

>100_bases
CAAGTAATATGGAGAGAAGGATGAGGATCCTTCTCTTTTTTTTACAACTTAAAAGGAAATTAGTAAAACTTTGATTGATG
AGGGGTGTTACAAAGAAAAG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVAILLDTKGPEIRTHDFVDG
QAELTTGSEVVISTEQVLGTAEKFSVTYAGLYDDVNPGSRILIDDGLIELEVIEKVDGNIRTKVLNSGIVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQNVDFIAASFVRKAADVLEIRELLEEHNAQHIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIATRVEKSLQYKDIFKKRIKELTPTITDAISQSVAHTAIALDVAAIIAPTESGYTAKMISKYRPKSPIVAVT
SDEQVCRRLALVWGVQAFMASKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEIAKGQ
GIGRKAAKGKVVVAKTATEAVANVKEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTE
TLKNGQEVTVDAARGIVYNGHAEVL

Sequences:

>Translated_585_residues
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVAILLDTKGPEIRTHDFVDG
QAELTTGSEVVISTEQVLGTAEKFSVTYAGLYDDVNPGSRILIDDGLIELEVIEKVDGNIRTKVLNSGIVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQNVDFIAASFVRKAADVLEIRELLEEHNAQHIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIATRVEKSLQYKDIFKKRIKELTPTITDAISQSVAHTAIALDVAAIIAPTESGYTAKMISKYRPKSPIVAVT
SDEQVCRRLALVWGVQAFMASKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEIAKGQ
GIGRKAAKGKVVVAKTATEAVANVKEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTE
TLKNGQEVTVDAARGIVYNGHAEVL
>Mature_585_residues
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVAILLDTKGPEIRTHDFVDG
QAELTTGSEVVISTEQVLGTAEKFSVTYAGLYDDVNPGSRILIDDGLIELEVIEKVDGNIRTKVLNSGIVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQNVDFIAASFVRKAADVLEIRELLEEHNAQHIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIATRVEKSLQYKDIFKKRIKELTPTITDAISQSVAHTAIALDVAAIIAPTESGYTAKMISKYRPKSPIVAVT
SDEQVCRRLALVWGVQAFMASKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEIAKGQ
GIGRKAAKGKVVVAKTATEAVANVKEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTE
TLKNGQEVTVDAARGIVYNGHAEVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=45.1020408163265, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=45.1020408163265, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=43.8775510204082, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=43.8775510204082, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=44.0816326530612, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=48.1099656357388, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=48.1099656357388, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=45.1882845188285, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=45.1882845188285, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=43.5779816513761, Blast_Score=167, Evalue=4e-41,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=50.9473684210526, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=40.3326403326403, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787994, Length=117, Percent_Identity=40.1709401709402, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=45.4545454545455, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=45.2479338842975, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=45.2479338842975, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=45.2479338842975, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=45.0413223140496, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=41.4285714285714, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=41.7695473251029, Blast_Score=351, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=44.5807770961145, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=44.5807770961145, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24648966, Length=392, Percent_Identity=47.7040816326531, Blast_Score=341, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=36.530612244898, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24646914, Length=249, Percent_Identity=46.5863453815261, Blast_Score=213, Evalue=4e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62367; Mature: 62367

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKT
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCE
VAILLDTKGPEIRTHDFVDGQAELTTGSEVVISTEQVLGTAEKFSVTYAGLYDDVNPGSR
EEEEEECCCCCCEECCCCCCCEEECCCCEEEEEHHHHHCCHHHHEEEEEECCCCCCCCCE
ILIDDGLIELEVIEKVDGNIRTKVLNSGIVKNKKGVNVPNVSIKLPGITEKDVKDIIFGI
EEEECCEEEEEEHHHCCCCCEEHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
EQNVDFIAASFVRKAADVLEIRELLEEHNAQHIQIVPKIENQEGIDNIDSILEVSDGLMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEE
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FDGTDAIMLSGETAAGQYPVEAVTMMANIATRVEKSLQYKDIFKKRIKELTPTITDAISQ
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHH
SVAHTAIALDVAAIIAPTESGYTAKMISKYRPKSPIVAVTSDEQVCRRLALVWGVQAFMA
HHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
SKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEIAKGQ
HHCCCCHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEECCCCCCEEEEEEECHHHHCCC
GIGRKAAKGKVVVAKTATEAVANVKEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHA
CCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCC
AVVGVSIGIPVIVGVNGVTETLKNGQEVTVDAARGIVYNGHAEVL
EEEEEEECCEEEEECCHHHHHHCCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKT
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCE
VAILLDTKGPEIRTHDFVDGQAELTTGSEVVISTEQVLGTAEKFSVTYAGLYDDVNPGSR
EEEEEECCCCCCEECCCCCCCEEECCCCEEEEEHHHHHCCHHHHEEEEEECCCCCCCCCE
ILIDDGLIELEVIEKVDGNIRTKVLNSGIVKNKKGVNVPNVSIKLPGITEKDVKDIIFGI
EEEECCEEEEEEHHHCCCCCEEHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
EQNVDFIAASFVRKAADVLEIRELLEEHNAQHIQIVPKIENQEGIDNIDSILEVSDGLMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEE
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FDGTDAIMLSGETAAGQYPVEAVTMMANIATRVEKSLQYKDIFKKRIKELTPTITDAISQ
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHH
SVAHTAIALDVAAIIAPTESGYTAKMISKYRPKSPIVAVTSDEQVCRRLALVWGVQAFMA
HHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
SKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEIAKGQ
HHCCCCHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEECCCCCCEEEEEEECHHHHCCC
GIGRKAAKGKVVVAKTATEAVANVKEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHA
CCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCC
AVVGVSIGIPVIVGVNGVTETLKNGQEVTVDAARGIVYNGHAEVL
EEEEEEECCEEEEECCHHHHHHCCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]