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The map label for this gene is grpE

Identifier: 152976745

GI number: 152976745

Start: 3088253

End: 3088849

Strand: Reverse

Name: grpE

Synonym: Bcer98_3041

Alternate gene names: 152976745

Gene position: 3088849-3088253 (Counterclockwise)

Preceding gene: 152976746

Following gene: 152976744

Centisome position: 75.58

GC content: 38.53

Gene sequence:

>597_bases
GTGGAAGAGCGTAACGAACAAGTGGTTGAAGAGACAAAAGAAGCGCAAACTGAAGAAGCTACTATAGAAAAAAACAGTGA
AGAATCTGTAACAGAAGAAGCAACTGAAGAAACTGTAGTAGAAGAAAAAAGTGAGGCGGCTCTTTTACAAGAAAAAGTAG
ATGAATTGCAAGCGAAATTGACGGAAACGGAAGGTCGCATGTTGCGTCTACAAGCTGATTTTGAAAATTACAAACGCCGT
GTTCAGCTTGATAAGCAAGCGGCGGAAAAATATAGAGCGCAAAGTCTTGTATCAGATATTTTACCTGCTCTTGATAATTT
TGAAAGAGCGATGCAAGTGGAAGCAAGTGATGAGCAAACGAAATCCTTATTACAAGGAATGGAAATGGTGTATCGTCAAT
TGTTAGAAGCGTTAAATAAAGAAGGTGTTGAGATGATTGAAGCTGTTGGAAAGCAGTTTGATCCTCATGAACACCAAGCT
GTTATGCAAGTAGAAGACAGCGAATTTGAATCTAACGCGGTTGTAGAAGAATTTCAAAAAGGTTATAAACTGAAGGACCG
TGTGATTCGCCCATCAATGGTAAAAGTAAATCAATAA

Upstream 100 bases:

>100_bases
GAGTAATGAAAGAAAATCATTGCTGGTGGGTAACATAAATATATGCTTGGGTGCTCATGCTCAGTGTGAGCACTTGAACA
CTTTTAAGGAGGTGAAAGTT

Downstream 100 bases:

>100_bases
TTTACATAAATGTAGGAGGTTTCGCCATGAGTAAAATTATCGGTATTGACTTAGGTACAACAAACTCTTGTGTAGCTGTT
ATGGAAGGTGGAGAACCAAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MEERNEQVVEETKEAQTEEATIEKNSEESVTEEATEETVVEEKSEAALLQEKVDELQAKLTETEGRMLRLQADFENYKRR
VQLDKQAAEKYRAQSLVSDILPALDNFERAMQVEASDEQTKSLLQGMEMVYRQLLEALNKEGVEMIEAVGKQFDPHEHQA
VMQVEDSEFESNAVVEEFQKGYKLKDRVIRPSMVKVNQ

Sequences:

>Translated_198_residues
MEERNEQVVEETKEAQTEEATIEKNSEESVTEEATEETVVEEKSEAALLQEKVDELQAKLTETEGRMLRLQADFENYKRR
VQLDKQAAEKYRAQSLVSDILPALDNFERAMQVEASDEQTKSLLQGMEMVYRQLLEALNKEGVEMIEAVGKQFDPHEHQA
VMQVEDSEFESNAVVEEFQKGYKLKDRVIRPSMVKVNQ
>Mature_198_residues
MEERNEQVVEETKEAQTEEATIEKNSEESVTEEATEETVVEEKSEAALLQEKVDELQAKLTETEGRMLRLQADFENYKRR
VQLDKQAAEKYRAQSLVSDILPALDNFERAMQVEASDEQTKSLLQGMEMVYRQLLEALNKEGVEMIEAVGKQFDPHEHQA
VMQVEDSEFESNAVVEEFQKGYKLKDRVIRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=187, Percent_Identity=33.6898395721925, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1788967, Length=194, Percent_Identity=32.9896907216495, Blast_Score=103, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324806, Length=178, Percent_Identity=31.4606741573034, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653432, Length=178, Percent_Identity=32.0224719101124, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BACCN (A7GT09)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376262.1
- ProteinModelPortal:   A7GT09
- SMR:   A7GT09
- STRING:   A7GT09
- EnsemblBacteria:   EBBACT00000037641
- GeneID:   5345304
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_3041
- eggNOG:   COG0576
- GeneTree:   EBGT00050000001213
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14140
- BioCyc:   BCER315749:BCER98_3041-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22847; Mature: 22847

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERNEQVVEETKEAQTEEATIEKNSEESVTEEATEETVVEEKSEAALLQEKVDELQAKL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TETEGRMLRLQADFENYKRRVQLDKQAAEKYRAQSLVSDILPALDNFERAMQVEASDEQT
HHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KSLLQGMEMVYRQLLEALNKEGVEMIEAVGKQFDPHEHQAVMQVEDSEFESNAVVEEFQK
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
GYKLKDRVIRPSMVKVNQ
CCHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MEERNEQVVEETKEAQTEEATIEKNSEESVTEEATEETVVEEKSEAALLQEKVDELQAKL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TETEGRMLRLQADFENYKRRVQLDKQAAEKYRAQSLVSDILPALDNFERAMQVEASDEQT
HHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KSLLQGMEMVYRQLLEALNKEGVEMIEAVGKQFDPHEHQAVMQVEDSEFESNAVVEEFQK
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
GYKLKDRVIRPSMVKVNQ
CCHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA