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The map label for this gene is bioK [H]

Identifier: 152976529

GI number: 152976529

Start: 2892880

End: 2894316

Strand: Reverse

Name: bioK [H]

Synonym: Bcer98_2818

Alternate gene names: 152976529

Gene position: 2894316-2892880 (Counterclockwise)

Preceding gene: 152976530

Following gene: 152976528

Centisome position: 70.82

GC content: 37.16

Gene sequence:

>1437_bases
ATGTTAACTTTATTTGTAAATAAGTTAACATTAGGGGTGACAATTATGACAGTAAGTAGTCGTACACGAGAAAAAATACC
GTATACATATGAAGAATTAGCGGAAAAGAATAAAGCGTATGTATGGCACCCATTTACACAAATGAAGGATTATATAGCAG
AAGATCCAGTTATTATTGAAAGAGGTGAAGGGCGTAAATTGTATGATGTGCATGGTAACGCTTACTGGGATGGAGTTTCT
TCCATTTGGCTTAATGTACATGGACATCACGTTCCAGAATTGGATGAAGCAATTCGTGAGCAATTAAATAAGATCGCTCA
TTCTACTATGTTAGGGCTTGCAAATGTACCGTCTATTTTACTTGCAGAAAAGGTTATTGAAGTTGTTCCAAAAGGGTTAA
AAAAGGTATTTTATTCAGATTCAGGTTCGACAGCTGTTGAGATTGCAATTAAAATGGCGTTTCAATATTGGCAACATAAA
GGGAAACCTAAAAAACAAAAGTTTATTACATTAAAAGAAGCATATCATGGTGATACGATTGGTGCTGTTTCTGTAGGAGC
AATCGACTTGTTTCACCAAGTGTATCGCTCTCTTTTATTTCATGCAATCAAAATGCCTTATCCGTCTACATATCGTTCTC
CTTATGGTAATAACGAGGAGGAAATCGTTCAAAAGCATTTGGAAGAAATGGAGGAATTATTGAAACAGAAACATGAAGAA
ATTGCAGCACTGATTGTAGAGCCTTTAATACAAGGGGCTGGCGGTATGATTACAATGCCAAAAGGGTATTTGAAAGGGCT
TCGTGATTTATGTACAAAGTATCATATTTTATTTATTACAGATGAAGTAGCAACAGGATTTGGCCGTACAGGAAAAATGT
TCGCCTGCGAGCATGAGGGAGTGACGCCGGATATATTAACAGCTGGAAAAGGATTAACTGGTGGATATTTACCGGTAGCG
ATTACCGTAACAACAGATGAGGTTTACAATGCTTTTTTAGGTGACTACGCAGAACAGAAAACCTTTTTTCATGGCCATAG
TTATACAGGAAATCCACTAGGTTGTGCAGTAGCGATTGCAAATCTAGAACTTTATGAAAAAACAAATGTAGTACAAAACG
TTGTAAAAAAGGCAGATTATGTAGCAAATAAATTGGCAGCGCTTTATGAGTATAAACATGTTGGTGATATTCGCCAACGT
GGCTTGATGATTGGGATAGAACTTGTTGCAAATAGGGAAACAAAAGAGCCGTTTGAATGGACAGAGCGGGTAGGAGTAGA
AGTATGTAAACGATCAAGAGAGCTTGGGATGATATTGCGCCCTCTTGGTAACACAATTGTATTCATGCCTCCGTTAGCAT
CTCAAATAGATGAACTAGATGAAATGTTACGCATTTTATATCAAGCAATTGCAGATATTACGGAGGGGAAATCATGA

Upstream 100 bases:

>100_bases
GGTAAGATCCAGCATGGAAGATGAAACGACTTATCATAGGATAAGTCGTTCTTTTTTAAGAACGAATCATTGAAAAGGAA
TTGACAGAGATTTCTCTTTA

Downstream 100 bases:

>100_bases
GTGGCTTTTTTATTACAGCAACGGATACCGAAGTAGGAAAAACAGTTGTTACAGGTGCAATAGCGGGGGGACTTAGAAAG
CTTGGACATCATATAGGAGT

Product: adenosylmethionine--8-amino-7-oxononanoate transaminase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLTLFVNKLTLGVTIMTVSSRTREKIPYTYEELAEKNKAYVWHPFTQMKDYIAEDPVIIERGEGRKLYDVHGNAYWDGVS
SIWLNVHGHHVPELDEAIREQLNKIAHSTMLGLANVPSILLAEKVIEVVPKGLKKVFYSDSGSTAVEIAIKMAFQYWQHK
GKPKKQKFITLKEAYHGDTIGAVSVGAIDLFHQVYRSLLFHAIKMPYPSTYRSPYGNNEEEIVQKHLEEMEELLKQKHEE
IAALIVEPLIQGAGGMITMPKGYLKGLRDLCTKYHILFITDEVATGFGRTGKMFACEHEGVTPDILTAGKGLTGGYLPVA
ITVTTDEVYNAFLGDYAEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNVVQNVVKKADYVANKLAALYEYKHVGDIRQR
GLMIGIELVANRETKEPFEWTERVGVEVCKRSRELGMILRPLGNTIVFMPPLASQIDELDEMLRILYQAIADITEGKS

Sequences:

>Translated_478_residues
MLTLFVNKLTLGVTIMTVSSRTREKIPYTYEELAEKNKAYVWHPFTQMKDYIAEDPVIIERGEGRKLYDVHGNAYWDGVS
SIWLNVHGHHVPELDEAIREQLNKIAHSTMLGLANVPSILLAEKVIEVVPKGLKKVFYSDSGSTAVEIAIKMAFQYWQHK
GKPKKQKFITLKEAYHGDTIGAVSVGAIDLFHQVYRSLLFHAIKMPYPSTYRSPYGNNEEEIVQKHLEEMEELLKQKHEE
IAALIVEPLIQGAGGMITMPKGYLKGLRDLCTKYHILFITDEVATGFGRTGKMFACEHEGVTPDILTAGKGLTGGYLPVA
ITVTTDEVYNAFLGDYAEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNVVQNVVKKADYVANKLAALYEYKHVGDIRQR
GLMIGIELVANRETKEPFEWTERVGVEVCKRSRELGMILRPLGNTIVFMPPLASQIDELDEMLRILYQAIADITEGKS
>Mature_478_residues
MLTLFVNKLTLGVTIMTVSSRTREKIPYTYEELAEKNKAYVWHPFTQMKDYIAEDPVIIERGEGRKLYDVHGNAYWDGVS
SIWLNVHGHHVPELDEAIREQLNKIAHSTMLGLANVPSILLAEKVIEVVPKGLKKVFYSDSGSTAVEIAIKMAFQYWQHK
GKPKKQKFITLKEAYHGDTIGAVSVGAIDLFHQVYRSLLFHAIKMPYPSTYRSPYGNNEEEIVQKHLEEMEELLKQKHEE
IAALIVEPLIQGAGGMITMPKGYLKGLRDLCTKYHILFITDEVATGFGRTGKMFACEHEGVTPDILTAGKGLTGGYLPVA
ITVTTDEVYNAFLGDYAEQKTFFHGHSYTGNPLGCAVAIANLELYEKTNVVQNVVKKADYVANKLAALYEYKHVGDIRQR
GLMIGIELVANRETKEPFEWTERVGVEVCKRSRELGMILRPLGNTIVFMPPLASQIDELDEMLRILYQAIADITEGKS

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=461, Percent_Identity=32.1041214750542, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI37574042, Length=444, Percent_Identity=29.2792792792793, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI226442705, Length=444, Percent_Identity=29.0540540540541, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI13994255, Length=462, Percent_Identity=27.7056277056277, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI284507298, Length=336, Percent_Identity=33.6309523809524, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI24119277, Length=433, Percent_Identity=27.9445727482679, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI226442709, Length=405, Percent_Identity=29.6296296296296, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI188536080, Length=318, Percent_Identity=27.3584905660377, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI38679950, Length=318, Percent_Identity=27.3584905660377, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI38679946, Length=318, Percent_Identity=27.3584905660377, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786991, Length=447, Percent_Identity=36.4653243847875, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI145693181, Length=418, Percent_Identity=32.7751196172249, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1788044, Length=414, Percent_Identity=33.0917874396135, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789759, Length=415, Percent_Identity=30.3614457831325, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789016, Length=422, Percent_Identity=29.6208530805687, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787560, Length=423, Percent_Identity=29.7872340425532, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786349, Length=312, Percent_Identity=29.8076923076923, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=465, Percent_Identity=30.5376344086021, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI32564660, Length=446, Percent_Identity=28.9237668161435, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI25144274, Length=272, Percent_Identity=34.9264705882353, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71992977, Length=489, Percent_Identity=25.7668711656442, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17541228, Length=312, Percent_Identity=27.8846153846154, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324386, Length=487, Percent_Identity=31.006160164271, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324432, Length=440, Percent_Identity=30.6818181818182, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6323470, Length=448, Percent_Identity=26.3392857142857, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321456, Length=394, Percent_Identity=26.6497461928934, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21357415, Length=461, Percent_Identity=32.3210412147505, Blast_Score=188, Evalue=6e-48,
Organism=Drosophila melanogaster, GI161085790, Length=449, Percent_Identity=31.6258351893096, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28574759, Length=451, Percent_Identity=31.2638580931264, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21356575, Length=425, Percent_Identity=28.9411764705882, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI281366494, Length=313, Percent_Identity=28.4345047923323, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24667139, Length=313, Percent_Identity=28.4345047923323, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24667143, Length=313, Percent_Identity=28.4345047923323, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 53773; Mature: 53773

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLFVNKLTLGVTIMTVSSRTREKIPYTYEELAEKNKAYVWHPFTQMKDYIAEDPVIIE
CEEEEEEHHHHCEEEEEECCCHHHCCCCCHHHHHHCCCCEEECCHHHHHHHHCCCCEEEE
RGEGRKLYDVHGNAYWDGVSSIWLNVHGHHVPELDEAIREQLNKIAHSTMLGLANVPSIL
CCCCCEEEEECCCCEECCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LAEKVIEVVPKGLKKVFYSDSGSTAVEIAIKMAFQYWQHKGKPKKQKFITLKEAYHGDTI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHCCCCC
GAVSVGAIDLFHQVYRSLLFHAIKMPYPSTYRSPYGNNEEEIVQKHLEEMEELLKQKHEE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAALIVEPLIQGAGGMITMPKGYLKGLRDLCTKYHILFITDEVATGFGRTGKMFACEHEG
HHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCEEEEEEEHHHHHCCCCCCCEEEECCCC
VTPDILTAGKGLTGGYLPVAITVTTDEVYNAFLGDYAEQKTFFHGHSYTGNPLGCAVAIA
CCCHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHH
NLELYEKTNVVQNVVKKADYVANKLAALYEYKHVGDIRQRGLMIGIELVANRETKEPFEW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHH
TERVGVEVCKRSRELGMILRPLGNTIVFMPPLASQIDELDEMLRILYQAIADITEGKS
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTLFVNKLTLGVTIMTVSSRTREKIPYTYEELAEKNKAYVWHPFTQMKDYIAEDPVIIE
CEEEEEEHHHHCEEEEEECCCHHHCCCCCHHHHHHCCCCEEECCHHHHHHHHCCCCEEEE
RGEGRKLYDVHGNAYWDGVSSIWLNVHGHHVPELDEAIREQLNKIAHSTMLGLANVPSIL
CCCCCEEEEECCCCEECCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LAEKVIEVVPKGLKKVFYSDSGSTAVEIAIKMAFQYWQHKGKPKKQKFITLKEAYHGDTI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHCCCCC
GAVSVGAIDLFHQVYRSLLFHAIKMPYPSTYRSPYGNNEEEIVQKHLEEMEELLKQKHEE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAALIVEPLIQGAGGMITMPKGYLKGLRDLCTKYHILFITDEVATGFGRTGKMFACEHEG
HHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCEEEEEEEHHHHHCCCCCCCEEEECCCC
VTPDILTAGKGLTGGYLPVAITVTTDEVYNAFLGDYAEQKTFFHGHSYTGNPLGCAVAIA
CCCHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHH
NLELYEKTNVVQNVVKKADYVANKLAALYEYKHVGDIRQRGLMIGIELVANRETKEPFEW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHH
TERVGVEVCKRSRELGMILRPLGNTIVFMPPLASQIDELDEMLRILYQAIADITEGKS
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]