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The map label for this gene is punA [H]

Identifier: 152976496

GI number: 152976496

Start: 2865535

End: 2866356

Strand: Reverse

Name: punA [H]

Synonym: Bcer98_2785

Alternate gene names: 152976496

Gene position: 2866356-2865535 (Counterclockwise)

Preceding gene: 152976497

Following gene: 152976495

Centisome position: 70.13

GC content: 38.69

Gene sequence:

>822_bases
ATGAATCGTGAACTTATTACAAAATCAGCAACATACTTAAAAGAGAAATTTCAAGAAACACCAAAGGTTGGATTAATCCT
TGGGTCTGGACTAGGTGTACTAGCAGATGAAATTGAAAATGCAGTGAAAGTACCATATAGTGAAATTCCTGAATTTCCTG
TATCAACGGTAGAGGGACATGCCGGACAACTTGTATTTGGTACACTTCAAGGTGTTACAGTAGTTGCAATGCAAGGGCGT
TTCCATTATTACGAAGGATATGACATGCAAAAAGTAACGTTCCCAGTTCGTGTGATGAAAGAACTAGGTGTAGAAACAGT
CGTTGTAACAAATGCAGCAGGCGGTGTAAATACATCATTTGAACCTGGCGATCTTATGTTAATTTCAGATCATATTAACT
TTATGGGACATAACCCATTAATTGGACCAAATGACGCTGAAATGGGTGTGCGATTCCCAGATATGTCTGAGTCTTACACA
AAAGAACTACGTGTAATGGTGAAACAAGTTGCCGAAGAGTTAAATATTAAAGTACAAGAAGGTGTATATGTTGCGATGAC
AGGTCCAGTATATGAAACACCTGCTGAGATTCGTATGCTTCGTACATTAGGCGGAGATGCAGTAGGAATGTCAACTGTGC
CAGAAGTTATTGTAGCGCGTCATGCTGGTATGAAAGTACTTGGAATTTCTTGTATTTCAAATATGGCAGCTGGTATTTTA
GATCAGCCACTTCATCATGATGAAGTGATTGAAACAACAGAACGTGTGAAAGCAAACTTCTTAGCATTGGTAAAAGCGAT
TGTAAAACAAATGAAGGGGTGA

Upstream 100 bases:

>100_bases
CGCTGATATTGGTGCAACTGTAGCAGAAAACTTCGGTGTAAACATGCCAGAATACGGAACAAGTTTCTTAAAGGAATTAA
AGAAATAGGAGGATATATTT

Downstream 100 bases:

>100_bases
GCTCACTATGAGAATGGTGGACCTAATTGCCAAAAAACGTGATGGACATGCGCTAACAACAGAAGAAATTAATTTTATTG
TTGAAGGATTTACAAACGGT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNRELITKSATYLKEKFQETPKVGLILGSGLGVLADEIENAVKVPYSEIPEFPVSTVEGHAGQLVFGTLQGVTVVAMQGR
FHYYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTSFEPGDLMLISDHINFMGHNPLIGPNDAEMGVRFPDMSESYT
KELRVMVKQVAEELNIKVQEGVYVAMTGPVYETPAEIRMLRTLGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGIL
DQPLHHDEVIETTERVKANFLALVKAIVKQMKG

Sequences:

>Translated_273_residues
MNRELITKSATYLKEKFQETPKVGLILGSGLGVLADEIENAVKVPYSEIPEFPVSTVEGHAGQLVFGTLQGVTVVAMQGR
FHYYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTSFEPGDLMLISDHINFMGHNPLIGPNDAEMGVRFPDMSESYT
KELRVMVKQVAEELNIKVQEGVYVAMTGPVYETPAEIRMLRTLGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGIL
DQPLHHDEVIETTERVKANFLALVKAIVKQMKG
>Mature_273_residues
MNRELITKSATYLKEKFQETPKVGLILGSGLGVLADEIENAVKVPYSEIPEFPVSTVEGHAGQLVFGTLQGVTVVAMQGR
FHYYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTSFEPGDLMLISDHINFMGHNPLIGPNDAEMGVRFPDMSESYT
KELRVMVKQVAEELNIKVQEGVYVAMTGPVYETPAEIRMLRTLGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGIL
DQPLHHDEVIETTERVKANFLALVKAIVKQMKG

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=258, Percent_Identity=53.4883720930233, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI47132622, Length=247, Percent_Identity=28.3400809716599, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788746, Length=252, Percent_Identity=48.4126984126984, Blast_Score=249, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17541190, Length=279, Percent_Identity=41.5770609318996, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71980569, Length=216, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323238, Length=301, Percent_Identity=43.1893687707641, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45552885, Length=277, Percent_Identity=48.014440433213, Blast_Score=253, Evalue=7e-68,
Organism=Drosophila melanogaster, GI45552887, Length=277, Percent_Identity=48.014440433213, Blast_Score=253, Evalue=9e-68,
Organism=Drosophila melanogaster, GI24656090, Length=277, Percent_Identity=48.014440433213, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24656093, Length=277, Percent_Identity=48.014440433213, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24762376, Length=233, Percent_Identity=39.9141630901288, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI221459247, Length=231, Percent_Identity=25.974025974026, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130079, Length=212, Percent_Identity=27.3584905660377, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29753; Mature: 29753

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRELITKSATYLKEKFQETPKVGLILGSGLGVLADEIENAVKVPYSEIPEFPVSTVEGH
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCHHCCCC
AGQLVFGTLQGVTVVAMQGRFHYYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTSF
CCCEEEEECCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
EPGDLMLISDHINFMGHNPLIGPNDAEMGVRFPDMSESYTKELRVMVKQVAEELNIKVQE
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEC
GVYVAMTGPVYETPAEIRMLRTLGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGIL
CEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DQPLHHDEVIETTERVKANFLALVKAIVKQMKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRELITKSATYLKEKFQETPKVGLILGSGLGVLADEIENAVKVPYSEIPEFPVSTVEGH
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCHHCCCC
AGQLVFGTLQGVTVVAMQGRFHYYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTSF
CCCEEEEECCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
EPGDLMLISDHINFMGHNPLIGPNDAEMGVRFPDMSESYTKELRVMVKQVAEELNIKVQE
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEC
GVYVAMTGPVYETPAEIRMLRTLGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGIL
CEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DQPLHHDEVIETTERVKANFLALVKAIVKQMKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]