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The map label for this gene is hdhA [C]

Identifier: 152976430

GI number: 152976430

Start: 2805752

End: 2806228

Strand: Reverse

Name: hdhA [C]

Synonym: Bcer98_2718

Alternate gene names: 152976430

Gene position: 2806228-2805752 (Counterclockwise)

Preceding gene: 152976431

Following gene: 152976428

Centisome position: 68.66

GC content: 37.32

Gene sequence:

>477_bases
ATGAAACTTGCAGGAAAGGTCATCGTTGTAACTGGTGGTGGTATTGGTATTGGACGTAGTACAGCTCTTTTATTGGCATC
AAACGGTGCCAAAGTGATTGTAACGGATATTAATAGAGAAAGCGGACAAGCGACAGCAGAAGAGATAGTAAATTTAGGTG
GAGAAGCACGATTTGTATCCCATAATATGGACAGACAGAGGGATTGGCAACATGTTATTGATGTCGCCATTGCTACATAT
CATCGCATTGATATGTTATTTCATAATGCTGTGCTATATAAAATTGACGCTATTTTCTTTGATCAACAAGGAAACGATTC
CAATGTACTTTGTATGAATGACGTTTCGATAGAAATAAAACAATTGACGTCATCATTTATGAAACAGCAAGAAGAAGTGA
TGCTAACGGATTTGCCTATCTTTGGTGTTATTAGTGCAAAGAAGCAATCATTTCATACAGTTGAAGCCCTGGTATAA

Upstream 100 bases:

>100_bases
TTCAAAGTTGCTGTCGAGGGAATGGCTGTTCCTGCTTGCGGATTTATCTAGGAAAGGAGTATACTGAAGGACGGTAGGAC
TTTTAGAAGGGGTAATGACA

Downstream 100 bases:

>100_bases
TAGTGGAAAACCGCCCTCGATTTGTGCGTGGACGGTTTTTTTGTATATTAGCGAATGCGTTTTTCTGTTTTGCAATCAAA
GAAATGTGCTTTATTTAAAT

Product: hypothetical protein

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKLAGKVIVVTGGGIGIGRSTALLLASNGAKVIVTDINRESGQATAEEIVNLGGEARFVSHNMDRQRDWQHVIDVAIATY
HRIDMLFHNAVLYKIDAIFFDQQGNDSNVLCMNDVSIEIKQLTSSFMKQQEEVMLTDLPIFGVISAKKQSFHTVEALV

Sequences:

>Translated_158_residues
MKLAGKVIVVTGGGIGIGRSTALLLASNGAKVIVTDINRESGQATAEEIVNLGGEARFVSHNMDRQRDWQHVIDVAIATY
HRIDMLFHNAVLYKIDAIFFDQQGNDSNVLCMNDVSIEIKQLTSSFMKQQEEVMLTDLPIFGVISAKKQSFHTVEALV
>Mature_158_residues
MKLAGKVIVVTGGGIGIGRSTALLLASNGAKVIVTDINRESGQATAEEIVNLGGEARFVSHNMDRQRDWQHVIDVAIATY
HRIDMLFHNAVLYKIDAIFFDQQGNDSNVLCMNDVSIEIKQLTSSFMKQQEEVMLTDLPIFGVISAKKQSFHTVEALV

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI1787905, Length=90, Percent_Identity=34.4444444444444, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 17376; Mature: 17376

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAGKVIVVTGGGIGIGRSTALLLASNGAKVIVTDINRESGQATAEEIVNLGGEARFVS
CCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
HNMDRQRDWQHVIDVAIATYHRIDMLFHNAVLYKIDAIFFDQQGNDSNVLCMNDVSIEIK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEECCCCHHH
QLTSSFMKQQEEVMLTDLPIFGVISAKKQSFHTVEALV
HHHHHHHHHHHHEEEECCCEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure
MKLAGKVIVVTGGGIGIGRSTALLLASNGAKVIVTDINRESGQATAEEIVNLGGEARFVS
CCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
HNMDRQRDWQHVIDVAIATYHRIDMLFHNAVLYKIDAIFFDQQGNDSNVLCMNDVSIEIK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEECCCCHHH
QLTSSFMKQQEEVMLTDLPIFGVISAKKQSFHTVEALV
HHHHHHHHHHHHEEEECCCEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]