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The map label for this gene is pbpB [H]

Identifier: 152976278

GI number: 152976278

Start: 2667981

End: 2670134

Strand: Reverse

Name: pbpB [H]

Synonym: Bcer98_2566

Alternate gene names: 152976278

Gene position: 2670134-2667981 (Counterclockwise)

Preceding gene: 152976279

Following gene: 152976277

Centisome position: 65.33

GC content: 38.95

Gene sequence:

>2154_bases
ATGATGAAGATGAACATGAACAAATTTCATACCAATAAGGGAGCAGGTTACTTTATGATATTTTTCCTCCTGCTCTTTTT
GCTGTTGTTAGCCCGATTCTTTTATATTCAAGCAACAGGAACAGTGCATAATCAAGATTTAAGGAAACTAGCTCAGGCTA
AACATAATAAAACAGGTGTACTAGAAGCAAACCGCGGTACCATTTATGATCAAAACGGTCATGTTCTTGCGCAGGATGCA
AACTCATATAAGCTTGTTGCGGTACTTAAAGGGGATAATGCCGTTAAAAACAAAGAAGAAGCGGCGAAAAAAATTGCTGG
TGTACTTGGTAAAGATGAAGAGAAAATTTTAACGATTCTAAAAAAAGACAAGAGCCAAGTAGAATTTGGACCGTTAGGAA
AGAATTTAACGAAAGAAACGAAAGAACAAATAGAAGCTTTAAAGATACCTGGAATTTCGTTTATTACAGAAAAAGCTAGA
GTATATCCAAATGGAGACTTCGCTTCTTACATACTAGGGTACTCCAGGATAAATGATCAAGGTACCACAGTAGGGGCGGG
AGGCTTAGAAAAGAGCTTAGATAAGTTTTTACGCGCTTCTAATGGAAGTGTAGGATATACAGGAGGGCATAACGGTGTTC
CTCTTGAAGGAGGAAAACAAAATATAAAGCCTCCTAAAAACGGCGATAATGTCTATTTGACAATTGATCAACGCATTCAA
AGCTTTTTAGAAAATGCGATGAAGGCAGCGGCAAAGCATTATGAGCCCGAAATGCTAACGGCAGTAGTCGCTGATCCGAA
AACAGGGAAAATATTAGCAATGTCTAGTAGCCCTAGCTATGATCCGAATAAACCAAAGATAGAGAACTACTTAAATATAC
CAGTTGCAACGACATTTGAGCCAGGATCGACAATGAAAATATTTACATTAGCAGCAGCAATTAATGAAGGAAAATATAAT
GGACAAGAATATTATCAATCCGGTACGTATCCAGTTGGCAATCGGAAAATAAAAGACCATAACGGCGGTGCTGGATGGGG
ATCCATTACATTTGATGAAGGAGTTGAACGTTCTTCTAACGTTGCATTTGCAATTTTGGGGGATAAAAAACTTGGTCCGG
ATTTATTCCGTCAATATATTCATAAGTTTGGCTTGGATGAAAAGACTGGCATCGATTTGCCCGGAGAAGTTGAAAATAAA
ATTGTATTTGATTCACAAATTCAGCAAGTAACAACTGCGTTTGGTCAAGGATCTACTGTAACTCCAATACAGCTTGTACA
AGCTGCAACAGCGATTGCCAATGACGGGAAAATGATGAAACCATATGTTATTGATCGAATTGTAGATCCGATAACAGGAA
AGGTAGAACTACAGCATAAACCAGAAGAGGTAGGGAAACCAGTTTCAAAAGAAACGGCAGCGCAAGTTAGACAACTATTA
GAACGTGTCGTTACATCGCCAAAAGGAACAGGGAATGCCTATAAGATTGACGGATATTCAGTAGGTGGTAAAACGGGTAC
AGCACAAATTCCTGATGGAAAAGGCGGATACATGACTGGTAGAGAAAATTATATTTTCTCTTTCCTCGGTATGGCACCGA
TGGATGATCCGCAATTGGTTGTGTACTTAGCAATTAAACAACCAAAACTAAAAGACGATGAATACGGAGCAAAGCCGCTT
GCTGAAATTTTCAAATCGGTAACAAAAGATAGCTTAGAATATTTAAAGATTAAGCCGAAGGAAGTAAAAGATCCGAAGAA
ATATGTGAAAGAACAGCAAGTAGTCGTTCCGGACGTTACAGGTAAAACAATGGAAGAAGCAAAAAAAATACTAGAAAAAG
CGAATTTTAGACCAACTATACTAGGGGAAGGTAAAGTGAAGCAACAAGTGCCGAAAGCAACTGAAAAGACATTAAAAGGT
GATCGTATTTTCTTAGTTGGTGAGCAACCTAAGATGCCGAATATCCAAGGCTGGGCACTGCGAGACGTGATGAATTTAGC
TAAAACGTTAAATCTCAATTTAAAACCTTCTGGAACAGGATATGTAACCGAGCAAAATGTTGAAGAAGGAGCATTGTTGC
AACCAGGGACAGAATTAGCTGTGACACTTATACCACCGCTTGAACCACAACAAGAGGCGGAGGAGCCGCAGTAG

Upstream 100 bases:

>100_bases
AGTCGTTATGAGCGAATCGCTAAAATTGCAAAAAAACACGGGCTAGAAATTAATGCAAATAATGTGAAAGGTTTACATTA
ATGCAAATAGGTGTGAAACA

Downstream 100 bases:

>100_bases
GAAATTGTGAAAGTGATAAACAAAAGTAGAAAGTTCTAATCAAATCGGTACAAGCATATATTGGAACAAGCTAGGCGTAA
GGGGGGCTTGTTCCAATATG

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MMKMNMNKFHTNKGAGYFMIFFLLLFLLLLARFFYIQATGTVHNQDLRKLAQAKHNKTGVLEANRGTIYDQNGHVLAQDA
NSYKLVAVLKGDNAVKNKEEAAKKIAGVLGKDEEKILTILKKDKSQVEFGPLGKNLTKETKEQIEALKIPGISFITEKAR
VYPNGDFASYILGYSRINDQGTTVGAGGLEKSLDKFLRASNGSVGYTGGHNGVPLEGGKQNIKPPKNGDNVYLTIDQRIQ
SFLENAMKAAAKHYEPEMLTAVVADPKTGKILAMSSSPSYDPNKPKIENYLNIPVATTFEPGSTMKIFTLAAAINEGKYN
GQEYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDKKLGPDLFRQYIHKFGLDEKTGIDLPGEVENK
IVFDSQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYVIDRIVDPITGKVELQHKPEEVGKPVSKETAAQVRQLL
ERVVTSPKGTGNAYKIDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYLAIKQPKLKDDEYGAKPL
AEIFKSVTKDSLEYLKIKPKEVKDPKKYVKEQQVVVPDVTGKTMEEAKKILEKANFRPTILGEGKVKQQVPKATEKTLKG
DRIFLVGEQPKMPNIQGWALRDVMNLAKTLNLNLKPSGTGYVTEQNVEEGALLQPGTELAVTLIPPLEPQQEAEEPQ

Sequences:

>Translated_717_residues
MMKMNMNKFHTNKGAGYFMIFFLLLFLLLLARFFYIQATGTVHNQDLRKLAQAKHNKTGVLEANRGTIYDQNGHVLAQDA
NSYKLVAVLKGDNAVKNKEEAAKKIAGVLGKDEEKILTILKKDKSQVEFGPLGKNLTKETKEQIEALKIPGISFITEKAR
VYPNGDFASYILGYSRINDQGTTVGAGGLEKSLDKFLRASNGSVGYTGGHNGVPLEGGKQNIKPPKNGDNVYLTIDQRIQ
SFLENAMKAAAKHYEPEMLTAVVADPKTGKILAMSSSPSYDPNKPKIENYLNIPVATTFEPGSTMKIFTLAAAINEGKYN
GQEYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDKKLGPDLFRQYIHKFGLDEKTGIDLPGEVENK
IVFDSQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYVIDRIVDPITGKVELQHKPEEVGKPVSKETAAQVRQLL
ERVVTSPKGTGNAYKIDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYLAIKQPKLKDDEYGAKPL
AEIFKSVTKDSLEYLKIKPKEVKDPKKYVKEQQVVVPDVTGKTMEEAKKILEKANFRPTILGEGKVKQQVPKATEKTLKG
DRIFLVGEQPKMPNIQGWALRDVMNLAKTLNLNLKPSGTGYVTEQNVEEGALLQPGTELAVTLIPPLEPQQEAEEPQ
>Mature_717_residues
MMKMNMNKFHTNKGAGYFMIFFLLLFLLLLARFFYIQATGTVHNQDLRKLAQAKHNKTGVLEANRGTIYDQNGHVLAQDA
NSYKLVAVLKGDNAVKNKEEAAKKIAGVLGKDEEKILTILKKDKSQVEFGPLGKNLTKETKEQIEALKIPGISFITEKAR
VYPNGDFASYILGYSRINDQGTTVGAGGLEKSLDKFLRASNGSVGYTGGHNGVPLEGGKQNIKPPKNGDNVYLTIDQRIQ
SFLENAMKAAAKHYEPEMLTAVVADPKTGKILAMSSSPSYDPNKPKIENYLNIPVATTFEPGSTMKIFTLAAAINEGKYN
GQEYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDKKLGPDLFRQYIHKFGLDEKTGIDLPGEVENK
IVFDSQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYVIDRIVDPITGKVELQHKPEEVGKPVSKETAAQVRQLL
ERVVTSPKGTGNAYKIDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYLAIKQPKLKDDEYGAKPL
AEIFKSVTKDSLEYLKIKPKEVKDPKKYVKEQQVVVPDVTGKTMEEAKKILEKANFRPTILGEGKVKQQVPKATEKTLKG
DRIFLVGEQPKMPNIQGWALRDVMNLAKTLNLNLKPSGTGYVTEQNVEEGALLQPGTELAVTLIPPLEPQQEAEEPQ

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=607, Percent_Identity=27.1828665568369, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1786854, Length=549, Percent_Identity=24.2258652094718, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 78670; Mature: 78670

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKMNMNKFHTNKGAGYFMIFFLLLFLLLLARFFYIQATGTVHNQDLRKLAQAKHNKTGV
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCE
LEANRGTIYDQNGHVLAQDANSYKLVAVLKGDNAVKNKEEAAKKIAGVLGKDEEKILTIL
EECCCCEEEECCCEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
KKDKSQVEFGPLGKNLTKETKEQIEALKIPGISFITEKARVYPNGDFASYILGYSRINDQ
HCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCEEECCCEECCCCCHHHHHHHHHHCCCC
GTTVGAGGLEKSLDKFLRASNGSVGYTGGHNGVPLEGGKQNIKPPKNGDNVYLTIDQRIQ
CCEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHH
SFLENAMKAAAKHYEPEMLTAVVADPKTGKILAMSSSPSYDPNKPKIENYLNIPVATTFE
HHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEEECCCCCCCCCCCCHHHEECCCEEEEEC
PGSTMKIFTLAAAINEGKYNGQEYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEEEEEEEHHCCCCCCCHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDKKLGPDLFRQYIHKFGLDEKTGIDLPGEVENKIVFDSQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYVIDRIVDPITGKVELQHKPEEVGKPVSKETAAQVRQLL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHH
ERVVTSPKGTGNAYKIDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
VYLAIKQPKLKDDEYGAKPLAEIFKSVTKDSLEYLKIKPKEVKDPKKYVKEQQVVVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCHHCCCHHHHHCCCCEEECCCC
GKTMEEAKKILEKANFRPTILGEGKVKQQVPKATEKTLKGDRIFLVGEQPKMPNIQGWAL
CHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLNLNLKPSGTGYVTEQNVEEGALLQPGTELAVTLIPPLEPQQEAEEPQ
HHHHHHHHHHCCEECCCCCCCEECCCCCCCCEECCCCCEEEEEECCCCCHHHCCCCC
>Mature Secondary Structure
MMKMNMNKFHTNKGAGYFMIFFLLLFLLLLARFFYIQATGTVHNQDLRKLAQAKHNKTGV
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCE
LEANRGTIYDQNGHVLAQDANSYKLVAVLKGDNAVKNKEEAAKKIAGVLGKDEEKILTIL
EECCCCEEEECCCEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
KKDKSQVEFGPLGKNLTKETKEQIEALKIPGISFITEKARVYPNGDFASYILGYSRINDQ
HCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCEEECCCEECCCCCHHHHHHHHHHCCCC
GTTVGAGGLEKSLDKFLRASNGSVGYTGGHNGVPLEGGKQNIKPPKNGDNVYLTIDQRIQ
CCEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHH
SFLENAMKAAAKHYEPEMLTAVVADPKTGKILAMSSSPSYDPNKPKIENYLNIPVATTFE
HHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEEECCCCCCCCCCCCHHHEECCCEEEEEC
PGSTMKIFTLAAAINEGKYNGQEYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEEEEEEEHHCCCCCCCHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDKKLGPDLFRQYIHKFGLDEKTGIDLPGEVENKIVFDSQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYVIDRIVDPITGKVELQHKPEEVGKPVSKETAAQVRQLL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHH
ERVVTSPKGTGNAYKIDGYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
VYLAIKQPKLKDDEYGAKPLAEIFKSVTKDSLEYLKIKPKEVKDPKKYVKEQQVVVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCHHCCCHHHHHCCCCEEECCCC
GKTMEEAKKILEKANFRPTILGEGKVKQQVPKATEKTLKGDRIFLVGEQPKMPNIQGWAL
CHHHHHHHHHHHHCCCCCEEECCCCHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLNLNLKPSGTGYVTEQNVEEGALLQPGTELAVTLIPPLEPQQEAEEPQ
HHHHHHHHHHCCEECCCCCCCEECCCCCCCCEECCCCCEEEEEECCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]