Click here to switch to the map view.

The map label for this gene is murD

Identifier: 152976274

GI number: 152976274

Start: 2661866

End: 2663221

Strand: Reverse

Name: murD

Synonym: Bcer98_2562

Alternate gene names: 152976274

Gene position: 2663221-2661866 (Counterclockwise)

Preceding gene: 152976275

Following gene: 152976273

Centisome position: 65.16

GC content: 36.8

Gene sequence:

>1356_bases
TTGAAAACGGTAACTGATTATCAAAATAAAAATATTCTTGTATTAGGTATTGCAAAAAGTGGGTATGCAGCAGCAAATTT
ATTAAAAAGCTTAGGAGCAAATGTCATTGTAAATGATGGGAAGCCTTTAGCGAATAATGAGTTAGCAGCAGAACTACAAG
CGAAAGGGATGGATGTAGTATGTGGAGGCCATCCATTAGAATTATTAGAAAGAAATATTGCGCTTGTAGTGAAAAATCCA
GGAATCCCATATTCCAACCCACTGCTAGTTGCAGCGACAGAGAAACAGATTCCTATTATCACGGAAATAGAATTAGCATA
TCGTATTTCAGAGGCGCCATTTATTGGTATTACAGGATCAAATGGTAAAACAACAACAACAATGCTAACGTTTGAAATGT
TAAAAGAGGGAGAAAAGCATCCCGCTATTGCTGGTAACATAGGAACAGTAGCATGTGAAGTAGCACAGGCGGCGAAAGCG
AATGAAGTGCTTGTGACAGAACTTTCATCATTTCAATTAATGGGTGTGGAGACATTTCAGCCTAAGATTGCTGCATTTTT
AAACTTATTTGAAGCACATTTAGATTACCATGGTACAAAAAAAGAATATGGTTTAGCGAAAGCGAATATTTTTAAAAACC
AGACAGCAGCAGATTATAGTGTGATAAATGCAGATGATGCGGATGTGATGGAATTATCTGCTAATAGCAAAGGGCAAAAG
ATATTGTTTTCTACTACAAAAGAAATTGAAGATGGCGCATGTATAAAAGAGAATGCTCTGTATTTTAAAGGTGAGAAAGT
TGTTGAAATCAAGGACATTGTTTTACCAGGTAAACACAACTTGGAAAATATTTTGGCGGCAATGAGTATTGCGAAATTGT
TAGGTGTAGCAAATGAGGCAATTGTTGCCGTGTTAAAACGATTCACAGGTGTAAAGCATCGTTTAGAATATGTAACAACG
ATTCATAACCGAAAGTTTTATAATGATTCAAAAGCAACGAATATATTGGCGACGGAGAAGGCATTATCAGCATTTACAAG
CCCTGTTATTCTACTTGCAGGCGGACTAGATCGCGGGAATGAATTTGATGATTTAATTCCGTATTTTGAACAACATGTAA
AAGCGATTGTAACTTATGGACAAACTGCACCGAAACTCGTTCATGCAGCTGAAAAAGCAGGGTTAGCAATTATTGAAGCT
GTCCATCATTTAGAAGAAGCGGTAGAAAGAGCATATGCTCACTCTGCTGATGGAGATGTTATCCTACTTTCACCAGCATG
CGCGAGCTGGGATCAATTTAAAACATTTGAAGAAAGAGGAGACATTTTTATACAAGCTGTGCATAAACTTATATAA

Upstream 100 bases:

>100_bases
TGAATTATGTGGTTGGTCAGAATGGCGTGTTGTTGTAACATTTTGGTCTGTAGGATTTTTACTAGCAGTATTAGGAATTT
ATATCGGGGTGTGGATGTAA

Downstream 100 bases:

>100_bases
AGTAAAACAAAACATCAGTAGGCTAACACCTACTGATGTTTTGTTACATAGGACAAAAATGCTTTGTCTATAAAGGTGGT
GGTCATTGCCAATGAAAAAA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKTVTDYQNKNILVLGIAKSGYAAANLLKSLGANVIVNDGKPLANNELAAELQAKGMDVVCGGHPLELLERNIALVVKNP
GIPYSNPLLVAATEKQIPIITEIELAYRISEAPFIGITGSNGKTTTTMLTFEMLKEGEKHPAIAGNIGTVACEVAQAAKA
NEVLVTELSSFQLMGVETFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTAADYSVINADDADVMELSANSKGQK
ILFSTTKEIEDGACIKENALYFKGEKVVEIKDIVLPGKHNLENILAAMSIAKLLGVANEAIVAVLKRFTGVKHRLEYVTT
IHNRKFYNDSKATNILATEKALSAFTSPVILLAGGLDRGNEFDDLIPYFEQHVKAIVTYGQTAPKLVHAAEKAGLAIIEA
VHHLEEAVERAYAHSADGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI

Sequences:

>Translated_451_residues
MKTVTDYQNKNILVLGIAKSGYAAANLLKSLGANVIVNDGKPLANNELAAELQAKGMDVVCGGHPLELLERNIALVVKNP
GIPYSNPLLVAATEKQIPIITEIELAYRISEAPFIGITGSNGKTTTTMLTFEMLKEGEKHPAIAGNIGTVACEVAQAAKA
NEVLVTELSSFQLMGVETFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTAADYSVINADDADVMELSANSKGQK
ILFSTTKEIEDGACIKENALYFKGEKVVEIKDIVLPGKHNLENILAAMSIAKLLGVANEAIVAVLKRFTGVKHRLEYVTT
IHNRKFYNDSKATNILATEKALSAFTSPVILLAGGLDRGNEFDDLIPYFEQHVKAIVTYGQTAPKLVHAAEKAGLAIIEA
VHHLEEAVERAYAHSADGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI
>Mature_451_residues
MKTVTDYQNKNILVLGIAKSGYAAANLLKSLGANVIVNDGKPLANNELAAELQAKGMDVVCGGHPLELLERNIALVVKNP
GIPYSNPLLVAATEKQIPIITEIELAYRISEAPFIGITGSNGKTTTTMLTFEMLKEGEKHPAIAGNIGTVACEVAQAAKA
NEVLVTELSSFQLMGVETFQPKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTAADYSVINADDADVMELSANSKGQK
ILFSTTKEIEDGACIKENALYFKGEKVVEIKDIVLPGKHNLENILAAMSIAKLLGVANEAIVAVLKRFTGVKHRLEYVTT
IHNRKFYNDSKATNILATEKALSAFTSPVILLAGGLDRGNEFDDLIPYFEQHVKAIVTYGQTAPKLVHAAEKAGLAIIEA
VHHLEEAVERAYAHSADGDVILLSPACASWDQFKTFEERGDIFIQAVHKLI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=461, Percent_Identity=32.9718004338395, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BACCN (A7GRN8)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001375791.1
- ProteinModelPortal:   A7GRN8
- SMR:   A7GRN8
- STRING:   A7GRN8
- EnsemblBacteria:   EBBACT00000039846
- GeneID:   5344536
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2562
- eggNOG:   COG0771
- GeneTree:   EBGT00070000032130
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- BioCyc:   BCER315749:BCER98_2562-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48871; Mature: 48871

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVTDYQNKNILVLGIAKSGYAAANLLKSLGANVIVNDGKPLANNELAAELQAKGMDVV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCEEE
CGGHPLELLERNIALVVKNPGIPYSNPLLVAATEKQIPIITEIELAYRISEAPFIGITGS
ECCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEEEEEEECCCCEEEEECC
NGKTTTTMLTFEMLKEGEKHPAIAGNIGTVACEVAQAAKANEVLVTELSSFQLMGVETFQ
CCCEEEHEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHCC
PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTAADYSVINADDADVMELSANSKGQK
HHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCCCE
ILFSTTKEIEDGACIKENALYFKGEKVVEIKDIVLPGKHNLENILAAMSIAKLLGVANEA
EEEEECCCCCCCCEEECCCEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
IVAVLKRFTGVKHRLEYVTTIHNRKFYNDSKATNILATEKALSAFTSPVILLAGGLDRGN
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEHHHHHHHHHCCCEEEEECCCCCCC
EFDDLIPYFEQHVKAIVTYGQTAPKLVHAAEKAGLAIIEAVHHLEEAVERAYAHSADGDV
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCE
ILLSPACASWDQFKTFEERGDIFIQAVHKLI
EEECCCCCCHHHHHHHHHHCHHHHHHHHHHC
>Mature Secondary Structure
MKTVTDYQNKNILVLGIAKSGYAAANLLKSLGANVIVNDGKPLANNELAAELQAKGMDVV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCEEE
CGGHPLELLERNIALVVKNPGIPYSNPLLVAATEKQIPIITEIELAYRISEAPFIGITGS
ECCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEEEEEEECCCCEEEEECC
NGKTTTTMLTFEMLKEGEKHPAIAGNIGTVACEVAQAAKANEVLVTELSSFQLMGVETFQ
CCCEEEHEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHCC
PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTAADYSVINADDADVMELSANSKGQK
HHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCCCE
ILFSTTKEIEDGACIKENALYFKGEKVVEIKDIVLPGKHNLENILAAMSIAKLLGVANEA
EEEEECCCCCCCCEEECCCEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
IVAVLKRFTGVKHRLEYVTTIHNRKFYNDSKATNILATEKALSAFTSPVILLAGGLDRGN
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEHHHHHHHHHCCCEEEEECCCCCCC
EFDDLIPYFEQHVKAIVTYGQTAPKLVHAAEKAGLAIIEAVHHLEEAVERAYAHSADGDV
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCE
ILLSPACASWDQFKTFEERGDIFIQAVHKLI
EEECCCCCCHHHHHHHHHHCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA