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The map label for this gene is rasP [H]

Identifier: 152976186

GI number: 152976186

Start: 2570448

End: 2571704

Strand: Reverse

Name: rasP [H]

Synonym: Bcer98_2472

Alternate gene names: 152976186

Gene position: 2571704-2570448 (Counterclockwise)

Preceding gene: 152976187

Following gene: 152976185

Centisome position: 62.92

GC content: 37.55

Gene sequence:

>1257_bases
TTGAATACAGCGATTGCCTTTATATTAATTTTTGGTGCACTCGTATTTTTCCATGAGCTAGGGCATCTCTATTTTGCAAA
AAGAGCAGGGATTTTATGTCGTGAGTTTGCGATTGGCTTTGGACCAAAAATCTTTTCCTTTGAAAAGAATGAAACGGTTT
ATACAGTTCGATTATTGCCTCTTGGCGGCTATGTTAGAATGGCTGGTGAAGATGCAGAAACAGTTGAATTAAAACCAGGT
ACAAAGGTTGGACTGGTATTAAATGAAAACGAAGAAGTTGTAAAGTTGGTTTTAGATAATCGGGAAAAATATCCGAATGT
CCGCGTCATAGAAGTAGAACAGGTGGACTTAGAACATAACCTTACAATCTCTGGTTACGAAGAATACGAAGAAGAAATTC
AAACATTCCGTGTGCATGAAAAAGCTCGCATTATTTCAACTGGAGAGGAAATCCAAATTGCGCCATTTCATAGACAATTT
GGTTCTAAAACATTGGGACAACGAGCATTAACAATTTTTGCAGGACCAGCTATGAACTTCATCCTTGCATTTGTTGTTTT
CGTCATCATCGGATTCATACAAGGTGTTCCTGCCGATAAGCCGATCATTGGGCAGGTAATGAAGGGGAGTATAGCTGAAC
AAGTGGGCTTGAAACCAAATGATATGATTCAAGCGATTGATGGGAAACATACACCGACTTGGAAAGATGTTGTAACTATT
GTCCGTGAAAGTCCCGATAAGGAAATTACGCTTCATGTAAAACGGGATAATGAACAAATTAATGTAAAAGTTACGCCAAC
AGCTGATCAAGAAGGGAAGGAAAAGGTTGGTAGAATTGGCGTAACTTCTTTAGTAGAAAAGTCTATTATCGGTTCTATTA
AATCAGGGTTTGAACAGACTTATACATGGACAAAACTCATCTTTGATTCCCTTGTAAAATTAGTTACCGGTCAATTTTCT
ATTAATGATTTATCAGGTCCGGTTGGGATTTATAATTTAACAGATCAAGTGGTGAACTATGGTGTTATACGTATTCTAAG
CTTGGCGGCAGTTTTAAGTATAAATCTTGGTTTATTTAACTTATTACCAGTTCCAGCTCTTGATGGAGGGCGCTTATTCT
TCTTCTTAATCGAAGCGTTGCGAGGTAAGCCAATCGACCGTCAAAAAGAAGGCATGGTTCACTTTATAGGATTTGCTTTA
TTGATGTTACTCATGTTAGTTGTAACGTGGAATGACATTCGAAAGTTTTTCTTGTAA

Upstream 100 bases:

>100_bases
ACAATGTCATTGCACGTCCGAGCTTAGAGGAAATTCAAGAAATTGATGCGGCCACAAGACGGTTTGTGATGGAACAAATT
TAGCAAAGGTGGTTATGGAA

Downstream 100 bases:

>100_bases
AATAGAATCAAATGAATAGTGAGTGTGAATTTCATCATGTCCGCTATGGATGTTGAATGAAATGAATTGAACACTGAGTT
GAGATCCCTCGCCGAATTTG

Product: putative membrane-associated zinc metalloprotease

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTVRLLPLGGYVRMAGEDAETVELKPG
TKVGLVLNENEEVVKLVLDNREKYPNVRVIEVEQVDLEHNLTISGYEEYEEEIQTFRVHEKARIISTGEEIQIAPFHRQF
GSKTLGQRALTIFAGPAMNFILAFVVFVIIGFIQGVPADKPIIGQVMKGSIAEQVGLKPNDMIQAIDGKHTPTWKDVVTI
VRESPDKEITLHVKRDNEQINVKVTPTADQEGKEKVGRIGVTSLVEKSIIGSIKSGFEQTYTWTKLIFDSLVKLVTGQFS
INDLSGPVGIYNLTDQVVNYGVIRILSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFAL
LMLLMLVVTWNDIRKFFL

Sequences:

>Translated_418_residues
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTVRLLPLGGYVRMAGEDAETVELKPG
TKVGLVLNENEEVVKLVLDNREKYPNVRVIEVEQVDLEHNLTISGYEEYEEEIQTFRVHEKARIISTGEEIQIAPFHRQF
GSKTLGQRALTIFAGPAMNFILAFVVFVIIGFIQGVPADKPIIGQVMKGSIAEQVGLKPNDMIQAIDGKHTPTWKDVVTI
VRESPDKEITLHVKRDNEQINVKVTPTADQEGKEKVGRIGVTSLVEKSIIGSIKSGFEQTYTWTKLIFDSLVKLVTGQFS
INDLSGPVGIYNLTDQVVNYGVIRILSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFAL
LMLLMLVVTWNDIRKFFL
>Mature_418_residues
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTVRLLPLGGYVRMAGEDAETVELKPG
TKVGLVLNENEEVVKLVLDNREKYPNVRVIEVEQVDLEHNLTISGYEEYEEEIQTFRVHEKARIISTGEEIQIAPFHRQF
GSKTLGQRALTIFAGPAMNFILAFVVFVIIGFIQGVPADKPIIGQVMKGSIAEQVGLKPNDMIQAIDGKHTPTWKDVVTI
VRESPDKEITLHVKRDNEQINVKVTPTADQEGKEKVGRIGVTSLVEKSIIGSIKSGFEQTYTWTKLIFDSLVKLVTGQFS
INDLSGPVGIYNLTDQVVNYGVIRILSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFAL
LMLLMLVVTWNDIRKFFL

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor [

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=231, Percent_Identity=29.8701298701299, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 46726; Mature: 46726

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTVRLLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEEEEEE
LGGYVRMAGEDAETVELKPGTKVGLVLNENEEVVKLVLDNREKYPNVRVIEVEQVDLEHN
CCCEEEECCCCCCEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
LTISGYEEYEEEIQTFRVHEKARIISTGEEIQIAPFHRQFGSKTLGQRALTIFAGPAMNF
CEECCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHCCHHHHHHEEEEEECHHHHH
ILAFVVFVIIGFIQGVPADKPIIGQVMKGSIAEQVGLKPNDMIQAIDGKHTPTWKDVVTI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHH
VRESPDKEITLHVKRDNEQINVKVTPTADQEGKEKVGRIGVTSLVEKSIIGSIKSGFEQT
HHCCCCCEEEEEEEECCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YTWTKLIFDSLVKLVTGQFSINDLSGPVGIYNLTDQVVNYGVIRILSLAAVLSINLGLFN
HHHHHHHHHHHHHHHCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFALLMLLMLVVTWNDIRKFFL
HCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTVRLLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEEEEEE
LGGYVRMAGEDAETVELKPGTKVGLVLNENEEVVKLVLDNREKYPNVRVIEVEQVDLEHN
CCCEEEECCCCCCEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
LTISGYEEYEEEIQTFRVHEKARIISTGEEIQIAPFHRQFGSKTLGQRALTIFAGPAMNF
CEECCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHCCHHHHHHEEEEEECHHHHH
ILAFVVFVIIGFIQGVPADKPIIGQVMKGSIAEQVGLKPNDMIQAIDGKHTPTWKDVVTI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHH
VRESPDKEITLHVKRDNEQINVKVTPTADQEGKEKVGRIGVTSLVEKSIIGSIKSGFEQT
HHCCCCCEEEEEEEECCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YTWTKLIFDSLVKLVTGQFSINDLSGPVGIYNLTDQVVNYGVIRILSLAAVLSINLGLFN
HHHHHHHHHHHHHHHCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFALLMLLMLVVTWNDIRKFFL
HCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]