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The map label for this gene is proS1 [H]

Identifier: 152976185

GI number: 152976185

Start: 2568599

End: 2570299

Strand: Reverse

Name: proS1 [H]

Synonym: Bcer98_2471

Alternate gene names: 152976185

Gene position: 2570299-2568599 (Counterclockwise)

Preceding gene: 152976186

Following gene: 152976184

Centisome position: 62.89

GC content: 38.33

Gene sequence:

>1701_bases
ATGAAACAAAGTATGGTGTTTAGTCCTACATTACGAGAAGTACCAGCAGATGCTGAAATTAAAAGTCATCAGTTACTGCT
TCGTGCAGGTTTTATGCGTCAAAATGCTTCAGGGATTTATAGTTTTCTGCCATTAGGGTTAAAAGTATTGCATAAAGTAG
AACACATTATTCGTGAAGAAATGGAACGTGCAGGAGCAGTAGAATTATTAATGCCTGCATTGCAAGCAGCAGAATTATGG
CAAGAATCAGGTCGTTGGTATTCTTACGGTTCTGAACTTATGCGTATGAAAGATCGTAATGATCGTGAATTTGCACTAGG
AGCAACGCATGAAGAAGTGATTACAGATCTTGTCCGTGATGAGATTAAGTCGTATAAAAAATTACCTTTAACGTTATATC
AAATTCAAACGAAATTCCGTGATGAACAACGCCCACGTTTTGGATTATTACGCGGAAGAGAATTTTTAATGAAAGATGCG
TATTCTTTCCATGCAACACAAGAAAGTTTAGATGAAGTGTATGATCGCTTATTCCAAGCGTATTCTAATATTTTTGCTCG
CTGTGGTCTGAACTTCCGTGCTGTTATTGCGGATTCTGGTGCTATGGGTGGTAAAGATACTCATGAATTTATGGTATTAT
CCGATGTAGGAGAGGACACAATCGCGTATTCTGATACATCTGATTATGCAGCCAATATTGAAATGGCTCCTGTTGTTGCT
ACTTATACAAAGAGCGATGAAGCGGAAAAAGCGCTTGAAAAGGTAGCGACGCCAGATCAAAAAGCAATTGAAGAAGTATC
TGCTTTCTTAAACATTGAACCATCTCAGTGTATAAAAACAATGGTATTTAAAGTAGATGAAAAATTTGTTGTTGTACTTG
TTCGCGGAGATCATGAAGTAAATGATGTAAAAGTGAAAAATGTATATGGCGCTTCAGTTGTCGAACTTGCTTCGTACGAA
GAGGTAAGAAGCCTCTTAAATTGCGAAGTAGGATCTTTAGGACCAATTGGGATAGCAGAGGATGTAGAAGTGATTGCTGA
CCATGCTGTAGCGGCAATTGTAAATGGTTGTTGCGGTGCAAATGAAGAAGGTTTCCATTATGTAAATGTAAATTCAGAGC
GTGACTTTAAAGTAAATCAATACGCAGATTTACGATTTATTCAAGAAGGCGATCAGTCTCCAGATGGAAAAGGTACAATT
CGTTTTGCTCGCGGAATTGAAGTTGGTCATGTCTTTAAATTAGGAACGCGCTATAGTGAAGCAATGAATGCAACATTCTT
AGACGAAAACGGAAAAACAAAACCACTTATTATGGGTTGTTATGGTATCGGTGTTTCTCGTACAGTAGCGGCGATTGCTG
AACAATTTAATGATGAGAATGGGTTAGTATGGCCAAAAGCGGTTGCACCGTTCCATGTACATGTTATTCCAGTTAATATG
AAGTCTGATGTACAACGTGAAGTTGCTGAAAATATTTACACTTCATTACAAGAGCAAGGATATGAAGTATTGCTAGATGA
TCGTACAGAACGTGCAGGCGTGAAATTTGCTGACGCTGATTTATTTGGACTACCAGTTCGTGTAACGGTTGGTAAGAAAG
CAGACGAAGGAATTGTAGAAGTAAAAGTGCGTGCGACAAACGAATCCGCAGAAGTAAAAGTGGAGGATCTTCACACGTAT
ATTGCCGATATTTTAAAATAA

Upstream 100 bases:

>100_bases
GATGTTGAATGAAATGAATTGAACACTGAGTTGAGATCCCTCGCCGAATTTGAGGTGAGGGTTTGTTATGTATTTGAGTA
AAATAAAGAGGTGCGAATAG

Downstream 100 bases:

>100_bases
AGTGAAACTTTAATCAGTGGGGTTTTCTTCATCCCCACTGATTATGAGCCCTCACCAATCGGGCTGTTACTGCCCGATTG
GTGAGAGAGAAAAAGGGGTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 1; ProRS 1 [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPLGLKVLHKVEHIIREEMERAGAVELLMPALQAAELW
QESGRWYSYGSELMRMKDRNDREFALGATHEEVITDLVRDEIKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLFQAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIEPSQCIKTMVFKVDEKFVVVLVRGDHEVNDVKVKNVYGASVVELASYE
EVRSLLNCEVGSLGPIGIAEDVEVIADHAVAAIVNGCCGANEEGFHYVNVNSERDFKVNQYADLRFIQEGDQSPDGKGTI
RFARGIEVGHVFKLGTRYSEAMNATFLDENGKTKPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDVQREVAENIYTSLQEQGYEVLLDDRTERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATNESAEVKVEDLHTY
IADILK

Sequences:

>Translated_566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPLGLKVLHKVEHIIREEMERAGAVELLMPALQAAELW
QESGRWYSYGSELMRMKDRNDREFALGATHEEVITDLVRDEIKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLFQAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIEPSQCIKTMVFKVDEKFVVVLVRGDHEVNDVKVKNVYGASVVELASYE
EVRSLLNCEVGSLGPIGIAEDVEVIADHAVAAIVNGCCGANEEGFHYVNVNSERDFKVNQYADLRFIQEGDQSPDGKGTI
RFARGIEVGHVFKLGTRYSEAMNATFLDENGKTKPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDVQREVAENIYTSLQEQGYEVLLDDRTERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATNESAEVKVEDLHTY
IADILK
>Mature_566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPLGLKVLHKVEHIIREEMERAGAVELLMPALQAAELW
QESGRWYSYGSELMRMKDRNDREFALGATHEEVITDLVRDEIKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLFQAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIEPSQCIKTMVFKVDEKFVVVLVRGDHEVNDVKVKNVYGASVVELASYE
EVRSLLNCEVGSLGPIGIAEDVEVIADHAVAAIVNGCCGANEEGFHYVNVNSERDFKVNQYADLRFIQEGDQSPDGKGTI
RFARGIEVGHVFKLGTRYSEAMNATFLDENGKTKPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDVQREVAENIYTSLQEQGYEVLLDDRTERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATNESAEVKVEDLHTY
IADILK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=43.3628318584071, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=51.3089005235602, Blast_Score=594, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI115532348, Length=242, Percent_Identity=36.7768595041322, Blast_Score=162, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=36.4705882352941, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=544, Percent_Identity=31.4338235294118, Blast_Score=246, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24656200, Length=256, Percent_Identity=33.59375, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63245; Mature: 63245

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPLGLKVLHKVEHIIREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MERAGAVELLMPALQAAELWQESGRWYSYGSELMRMKDRNDREFALGATHEEVITDLVRD
HHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
EIKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLFQA
HHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEECCEEE
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIEPSQCIKTMVFKVDEKFVVVLVRGDHEV
EECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCC
NDVKVKNVYGASVVELASYEEVRSLLNCEVGSLGPIGIAEDVEVIADHAVAAIVNGCCGA
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
NEEGFHYVNVNSERDFKVNQYADLRFIQEGDQSPDGKGTIRFARGIEVGHVFKLGTRYSE
CCCCEEEEEECCCCCEECCCHHHHHEECCCCCCCCCCCEEEEECCCCCCCEEECCCHHHH
AMNATFLDENGKTKPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECC
KSDVQREVAENIYTSLQEQGYEVLLDDRTERAGVKFADADLFGLPVRVTVGKKADEGIVE
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCEECCEEEEEECCCCCCCEEE
VKVRATNESAEVKVEDLHTYIADILK
EEEEECCCCCEEEHHHHHHHHHHHHC
>Mature Secondary Structure
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPLGLKVLHKVEHIIREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MERAGAVELLMPALQAAELWQESGRWYSYGSELMRMKDRNDREFALGATHEEVITDLVRD
HHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
EIKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLFQA
HHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEECCEEE
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIEPSQCIKTMVFKVDEKFVVVLVRGDHEV
EECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCC
NDVKVKNVYGASVVELASYEEVRSLLNCEVGSLGPIGIAEDVEVIADHAVAAIVNGCCGA
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
NEEGFHYVNVNSERDFKVNQYADLRFIQEGDQSPDGKGTIRFARGIEVGHVFKLGTRYSE
CCCCEEEEEECCCCCEECCCHHHHHEECCCCCCCCCCCEEEEECCCCCCCEEECCCHHHH
AMNATFLDENGKTKPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECC
KSDVQREVAENIYTSLQEQGYEVLLDDRTERAGVKFADADLFGLPVRVTVGKKADEGIVE
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCEECCEEEEEECCCCCCCEEE
VKVRATNESAEVKVEDLHTYIADILK
EEEEECCCCCEEEHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA