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The map label for this gene is fruK [H]

Identifier: 152976111

GI number: 152976111

Start: 2482443

End: 2483354

Strand: Reverse

Name: fruK [H]

Synonym: Bcer98_2395

Alternate gene names: 152976111

Gene position: 2483354-2482443 (Counterclockwise)

Preceding gene: 152976112

Following gene: 152976110

Centisome position: 60.76

GC content: 36.29

Gene sequence:

>912_bases
ATGATCTATACAGTGACATTAAATCCATCTATTGATTATGTTGTTCAAGTAGAGCCCTTTATGTTAGGAACTGTGAACAG
AGCGGAAAAAGATATGAAATTCCCCGGAGGAAAAGGAATTAATGTATCACGCGTACTCCATCGTCTTGGTGTTCAAAATA
TTGCTCTTGGTTTTACAGGAGGATTTACAGGAGAATTCATTAAAAGTGTATTGAAAAGTGAAGATATTAATACTGATTTT
GTTCAAGTAGATGGAGATTCAAGAATCAATGTGAAAATAAAAGGAAAAGAAGAAACGGAATTAAATGGCCAAGGTCCAAT
AATTACAGAAGAGCAGTTTCAGCAGCTTATGAAGAAGTTTGAAAAGATGCGAGCGGGGGATTTTGTTGTATTGGCTGGAA
GCATTCCGTCCTCTATTCCTAAAAATTTCTATGAACAAATAGCGATGTATGGAGAAGAACGTGGTATTCAAGTAGTTGTT
GATACAAGTGGTAGTGCTTTAAAGCATGTTATGAAAAATAACCCTTTTCTATTGAAGCCAAATCATTATGAACTTGGTGA
ATTGTTTGGAAAGGAACTTTCAACTGTAGAAGACATCCTTCCGTATGGAAAGAAATTAATTGCACAAGGTGTACAAAATG
TTATTGTTTCTATGGCAGGAGAGGGCGCATTATTGTTTACAAATGATGGTGTGTATGAGGCAACTGTACCGAAAGGACAA
GTTGTGAATTCCGTTGGAGCAGGAGACTCTCTTGTTGCAGGGTTTGTAGGAATATATGAACAAACAAAAGATATAATAAA
AGCATTTCAATATGGTGTTGCAACAGGAAGTGCAACCGCGTTCTCAGCAGACTTATGTACGAAAGACAAGGTAGAAACAT
TATTATCGCAAGTAATCGTTACAAAACGATAG

Upstream 100 bases:

>100_bases
CTAAGATTGCTAATATTGAAGATGCGAAAATGATTACTAACGCACTAGAAGAGATAGAAGAATATAGGAAAAAAACGAAT
GTGATTGAGGTTGATAAATC

Downstream 100 bases:

>100_bases
GGGGAACAGTATATGAAAATTACAGAATTGCTAAAAAAAGATACCATTATTATGGATTTGACAGCTTCAGATAAAGAAGC
TGTCATTGATGAATTAGTAG

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIYTVTLNPSIDYVVQVEPFMLGTVNRAEKDMKFPGGKGINVSRVLHRLGVQNIALGFTGGFTGEFIKSVLKSEDINTDF
VQVDGDSRINVKIKGKEETELNGQGPIITEEQFQQLMKKFEKMRAGDFVVLAGSIPSSIPKNFYEQIAMYGEERGIQVVV
DTSGSALKHVMKNNPFLLKPNHYELGELFGKELSTVEDILPYGKKLIAQGVQNVIVSMAGEGALLFTNDGVYEATVPKGQ
VVNSVGAGDSLVAGFVGIYEQTKDIIKAFQYGVATGSATAFSADLCTKDKVETLLSQVIVTKR

Sequences:

>Translated_303_residues
MIYTVTLNPSIDYVVQVEPFMLGTVNRAEKDMKFPGGKGINVSRVLHRLGVQNIALGFTGGFTGEFIKSVLKSEDINTDF
VQVDGDSRINVKIKGKEETELNGQGPIITEEQFQQLMKKFEKMRAGDFVVLAGSIPSSIPKNFYEQIAMYGEERGIQVVV
DTSGSALKHVMKNNPFLLKPNHYELGELFGKELSTVEDILPYGKKLIAQGVQNVIVSMAGEGALLFTNDGVYEATVPKGQ
VVNSVGAGDSLVAGFVGIYEQTKDIIKAFQYGVATGSATAFSADLCTKDKVETLLSQVIVTKR
>Mature_303_residues
MIYTVTLNPSIDYVVQVEPFMLGTVNRAEKDMKFPGGKGINVSRVLHRLGVQNIALGFTGGFTGEFIKSVLKSEDINTDF
VQVDGDSRINVKIKGKEETELNGQGPIITEEQFQQLMKKFEKMRAGDFVVLAGSIPSSIPKNFYEQIAMYGEERGIQVVV
DTSGSALKHVMKNNPFLLKPNHYELGELFGKELSTVEDILPYGKKLIAQGVQNVIVSMAGEGALLFTNDGVYEATVPKGQ
VVNSVGAGDSLVAGFVGIYEQTKDIIKAFQYGVATGSATAFSADLCTKDKVETLLSQVIVTKR

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=300, Percent_Identity=28.6666666666667, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788493, Length=258, Percent_Identity=31.3953488372093, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 32973; Mature: 32973

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTVTLNPSIDYVVQVEPFMLGTVNRAEKDMKFPGGKGINVSRVLHRLGVQNIALGFTG
CEEEEEECCCCCEEEEECCEEEECCHHHHHHCCCCCCCCCCHHHHHHHHCHHHEEEEECC
GFTGEFIKSVLKSEDINTDFVQVDGDSRINVKIKGKEETELNGQGPIITEEQFQQLMKKF
CCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCEECHHHHHHHHHHH
EKMRAGDFVVLAGSIPSSIPKNFYEQIAMYGEERGIQVVVDTSGSALKHVMKNNPFLLKP
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCEEECC
NHYELGELFGKELSTVEDILPYGKKLIAQGVQNVIVSMAGEGALLFTNDGVYEATVPKGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCH
VVNSVGAGDSLVAGFVGIYEQTKDIIKAFQYGVATGSATAFSADLCTKDKVETLLSQVIV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHCCCHHHHHHHHHHHHH
TKR
CCC
>Mature Secondary Structure
MIYTVTLNPSIDYVVQVEPFMLGTVNRAEKDMKFPGGKGINVSRVLHRLGVQNIALGFTG
CEEEEEECCCCCEEEEECCEEEECCHHHHHHCCCCCCCCCCHHHHHHHHCHHHEEEEECC
GFTGEFIKSVLKSEDINTDFVQVDGDSRINVKIKGKEETELNGQGPIITEEQFQQLMKKF
CCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCEECHHHHHHHHHHH
EKMRAGDFVVLAGSIPSSIPKNFYEQIAMYGEERGIQVVVDTSGSALKHVMKNNPFLLKP
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCEEECC
NHYELGELFGKELSTVEDILPYGKKLIAQGVQNVIVSMAGEGALLFTNDGVYEATVPKGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCH
VVNSVGAGDSLVAGFVGIYEQTKDIIKAFQYGVATGSATAFSADLCTKDKVETLLSQVIV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHCCCHHHHHHHHHHHHH
TKR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]