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The map label for this gene is miaA

Identifier: 152976107

GI number: 152976107

Start: 2477945

End: 2478895

Strand: Reverse

Name: miaA

Synonym: Bcer98_2391

Alternate gene names: 152976107

Gene position: 2478895-2477945 (Counterclockwise)

Preceding gene: 152976109

Following gene: 152976106

Centisome position: 60.65

GC content: 36.49

Gene sequence:

>951_bases
ATGGAAGAAAAGCAACGTGAGAAAGTTGTTGTCATCATCGGTCCGACAGCTGTAGGAAAGACGAAGCTTAGCATTGAACT
TGCAAAAGCGTTGAATGGTGAAATTGTTAGTGGAGACTCTATGCAAATTTATCGTACGATGGATATTGGGACTGCTAAAG
TAACAGCTGATGAAATGGAAGGGATTCCGCATTATATGATTGATATTAAGGATCCAGAAGATTCGTTTTCTGTAGCGGAA
TTTCAAGAGCTTGTTCGCGGACATATTCGAGAAATTACAAAACGTGGGAAATTGCCGATTATTGTTGGTGGAACGGGTCT
TTATATCCAATCTGTTCTATATGATTATCAATTTACAGATGAAGCAGGAGATACTGCATATAGAGAACGAATGGAAAAAT
TAGCTGAAGAACAAGGGGTAGAATTTGTACATAAAAAATTAGAAGAAGTAGATCCAGAAAGTGCAAAGCGTATTCATGCT
AACAATGTAAGGCGTGTGATTCGAGCGCTAGAAATTTTCCAGACAACCGGGAAAAAAATGAGTGAGCAACTTGAAAAACA
AAAAAATGAATTATTATATGATGTTGTCTTAATTGGCTTGACAATGGATCGTACAATGCTATATGATCGCATCAACTTAC
GTGTTGATCTTATGATGGAACAAGGGTTAGAGCAAGAAGTGAAACGCTTATATCAAAATGGTGTACGAGATTGTCAATCT
ATTCAAGCAATTGGTTATAAAGAGTTATATCGTTATTTTGCAGGAGCTACCTCGTTAGAAGAGGCGATATCGCAATTGAA
AACAAATTCACGTCGATATGCGAAACGTCAATTAACTTGGTTTCGTAATAAAATGGATGTAGCATGGTTTGATGTTACAG
ATGGTGAAAAAACAGCAGAAATTTTGCGATATATAGAAGGAAAGCTCCAACTAAAGTCGAATAATAATTGA

Upstream 100 bases:

>100_bases
AATTTCATTTACCGAATTGAAAGAACTTGCATTTTAACAGTTAATATTGGTAAGATTGGATATAATGACGGAGTACATTA
CATAAAGAGAGTTGAAATAT

Downstream 100 bases:

>100_bases
GTAGAGAAAAAGAGGAGGATTCGACATGAAGCAATCAATCAATATTCAAGATCAATTTTTAAATCAACTACGTAAAGAAA
ATACGTTTGTGACGCTGTAC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MEEKQREKVVVIIGPTAVGKTKLSIELAKALNGEIVSGDSMQIYRTMDIGTAKVTADEMEGIPHYMIDIKDPEDSFSVAE
FQELVRGHIREITKRGKLPIIVGGTGLYIQSVLYDYQFTDEAGDTAYRERMEKLAEEQGVEFVHKKLEEVDPESAKRIHA
NNVRRVIRALEIFQTTGKKMSEQLEKQKNELLYDVVLIGLTMDRTMLYDRINLRVDLMMEQGLEQEVKRLYQNGVRDCQS
IQAIGYKELYRYFAGATSLEEAISQLKTNSRRYAKRQLTWFRNKMDVAWFDVTDGEKTAEILRYIEGKLQLKSNNN

Sequences:

>Translated_316_residues
MEEKQREKVVVIIGPTAVGKTKLSIELAKALNGEIVSGDSMQIYRTMDIGTAKVTADEMEGIPHYMIDIKDPEDSFSVAE
FQELVRGHIREITKRGKLPIIVGGTGLYIQSVLYDYQFTDEAGDTAYRERMEKLAEEQGVEFVHKKLEEVDPESAKRIHA
NNVRRVIRALEIFQTTGKKMSEQLEKQKNELLYDVVLIGLTMDRTMLYDRINLRVDLMMEQGLEQEVKRLYQNGVRDCQS
IQAIGYKELYRYFAGATSLEEAISQLKTNSRRYAKRQLTWFRNKMDVAWFDVTDGEKTAEILRYIEGKLQLKSNNN
>Mature_316_residues
MEEKQREKVVVIIGPTAVGKTKLSIELAKALNGEIVSGDSMQIYRTMDIGTAKVTADEMEGIPHYMIDIKDPEDSFSVAE
FQELVRGHIREITKRGKLPIIVGGTGLYIQSVLYDYQFTDEAGDTAYRERMEKLAEEQGVEFVHKKLEEVDPESAKRIHA
NNVRRVIRALEIFQTTGKKMSEQLEKQKNELLYDVVLIGLTMDRTMLYDRINLRVDLMMEQGLEQEVKRLYQNGVRDCQS
IQAIGYKELYRYFAGATSLEEAISQLKTNSRRYAKRQLTWFRNKMDVAWFDVTDGEKTAEILRYIEGKLQLKSNNN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=33.968253968254, Blast_Score=167, Evalue=8e-42,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=39.1156462585034, Blast_Score=220, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=32.6732673267327, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=36.4820846905537, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24649830, Length=336, Percent_Identity=31.8452380952381, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BACCN (A7GR71)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001375624.1
- ProteinModelPortal:   A7GR71
- SMR:   A7GR71
- STRING:   A7GR71
- EnsemblBacteria:   EBBACT00000039124
- GeneID:   5343829
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_2391
- eggNOG:   COG0324
- GeneTree:   EBGT00050000001497
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   BCER315749:BCER98_2391-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36423; Mature: 36423

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKQREKVVVIIGPTAVGKTKLSIELAKALNGEIVSGDSMQIYRTMDIGTAKVTADEME
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCEEEHHHHC
GIPHYMIDIKDPEDSFSVAEFQELVRGHIREITKRGKLPIIVGGTGLYIQSVLYDYQFTD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCC
EAGDTAYRERMEKLAEEQGVEFVHKKLEEVDPESAKRIHANNVRRVIRALEIFQTTGKKM
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEQLEKQKNELLYDVVLIGLTMDRTMLYDRINLRVDLMMEQGLEQEVKRLYQNGVRDCQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IQAIGYKELYRYFAGATSLEEAISQLKTNSRRYAKRQLTWFRNKMDVAWFDVTDGEKTAE
HHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
ILRYIEGKLQLKSNNN
HHHHHCCEEEEECCCC
>Mature Secondary Structure
MEEKQREKVVVIIGPTAVGKTKLSIELAKALNGEIVSGDSMQIYRTMDIGTAKVTADEME
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCEEEHHHHC
GIPHYMIDIKDPEDSFSVAEFQELVRGHIREITKRGKLPIIVGGTGLYIQSVLYDYQFTD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCC
EAGDTAYRERMEKLAEEQGVEFVHKKLEEVDPESAKRIHANNVRRVIRALEIFQTTGKKM
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEQLEKQKNELLYDVVLIGLTMDRTMLYDRINLRVDLMMEQGLEQEVKRLYQNGVRDCQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IQAIGYKELYRYFAGATSLEEAISQLKTNSRRYAKRQLTWFRNKMDVAWFDVTDGEKTAE
HHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
ILRYIEGKLQLKSNNN
HHHHHCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA