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The map label for this gene is dctA [H]

Identifier: 152975971

GI number: 152975971

Start: 2343305

End: 2344546

Strand: Reverse

Name: dctA [H]

Synonym: Bcer98_2244

Alternate gene names: 152975971

Gene position: 2344546-2343305 (Counterclockwise)

Preceding gene: 152975972

Following gene: 152975970

Centisome position: 57.37

GC content: 36.07

Gene sequence:

>1242_bases
ATGAGAATTGTAAAAAACTTAACATTTCAAGTGATCGTGGCAATTATTTGCGGGATTGCAGTAGGAGCAATTTGGCCTAG
TGTTGGGCAACAAATGAAACCGATTGGTGAAACATTTATTAATATGATTAAAATGGTTATTGCACCGATTATCTTTTTAA
CAATCGTCCTTGGTATTGCAAGTATGGGAAATATGAAAAAAGTGGGACGCGTTGGAGGAAAGGCACTGTTATATTTTGAA
ATTGTAACGACACTTGCACTTTTAATAGGGATTATTGTTGCAAATGCTGTACGACCTGGTGATGGATTGGATCCCACAAA
GTTAAAGGGGGGCGATGTGTCACAATACGTACAAAGTGGTCAAGAAATGAAGTGGCTCGATTTCTTTTTACATATTGTAC
CATCTAATATGTTTGAAGCCTTTGTAAAAGGGGATATTTTGCAAGTTCTATTCTTTTCTATTTTATTTGGAGCAGGACTA
ACAATGCTCGGAAAAAATGGACAGCCGGTTATCGATTTTTTTGAACGATTATCGAAGGTCTTTTTCAACATTTTATCGAT
TGTTATGAAATTGGCGCCTCTTGGAGCTTTTGGTGGAATGGCGTTTACAATTGGAAAGTACGGTTTAAGCACATTAATTC
CGCTTAGTAAGTTAATGGTTTGTGTCTATGCGACGATGGCATTATTCGTTTTTATCGTTTTACATTTTATTTGTCGATTG
TATAAATTTAGTTTATGGAAATATTTATCGTATATAAAAGAAGAGTTGCTTATCGTCCTTGGGACATCATCTTCAGAGTC
CGTATTGCCACGAATGATGACAAAGATGGAACAGTTCGGCTGCTCTAAACCGGTAGTAGGTTTAGTGATTCCAACAGGAT
ATTCATTTAACTTAGATGGAACAACCATTTATTTATCAATGGCAACGATCTTTTTAGCACAAGTTTTTCATATTGATCTT
TCACTAGGGCAACAATTAACAATTATAGCTATTTTGTTAGTAACATCAAAAGGAGCAGCTGCTGTAACTGGTGGGGGTTT
TATCGTACTTGCTTCCACTTTATCTGCGATGAATGTGATTCCTTTAGAAGGACTTGCACTTTTACTTGGCGTTGACCGTT
TTATGTCAGAAGCGCGTGCGATTGTCAATTTAATTGGTAATGGCATTGCGACTGTCGTTGTTGCAAAAAGTGAAAATGAA
TTTAGTGAAGAAAAATATAAACAAGTGTTAATTCGTATGTAG

Upstream 100 bases:

>100_bases
TGGAGTTGAAACGGGGAAATAAGGGGGATTTTATAAAAAAGATTGAGGGGTTCTTTTCTATAAGCAGAGGAAAAATGAGT
AAACAGGGGAGTAGAAACAA

Downstream 100 bases:

>100_bases
AAAGAGGCTATGTCAAAAGTTACTGGTGATTCAGCAACTTTTGAGAGCCTCTTTTATTTTTGTAATAAAAAAATTATCGT
TTATGCATTTTTTATAAGTA

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQQMKPIGETFINMIKMVIAPIIFLTIVLGIASMGNMKKVGRVGGKALLYFE
IVTTLALLIGIIVANAVRPGDGLDPTKLKGGDVSQYVQSGQEMKWLDFFLHIVPSNMFEAFVKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPLGAFGGMAFTIGKYGLSTLIPLSKLMVCVYATMALFVFIVLHFICRL
YKFSLWKYLSYIKEELLIVLGTSSSESVLPRMMTKMEQFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHIDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FSEEKYKQVLIRM

Sequences:

>Translated_413_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQQMKPIGETFINMIKMVIAPIIFLTIVLGIASMGNMKKVGRVGGKALLYFE
IVTTLALLIGIIVANAVRPGDGLDPTKLKGGDVSQYVQSGQEMKWLDFFLHIVPSNMFEAFVKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPLGAFGGMAFTIGKYGLSTLIPLSKLMVCVYATMALFVFIVLHFICRL
YKFSLWKYLSYIKEELLIVLGTSSSESVLPRMMTKMEQFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHIDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FSEEKYKQVLIRM
>Mature_413_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQQMKPIGETFINMIKMVIAPIIFLTIVLGIASMGNMKKVGRVGGKALLYFE
IVTTLALLIGIIVANAVRPGDGLDPTKLKGGDVSQYVQSGQEMKWLDFFLHIVPSNMFEAFVKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPLGAFGGMAFTIGKYGLSTLIPLSKLMVCVYATMALFVFIVLHFICRL
YKFSLWKYLSYIKEELLIVLGTSSSESVLPRMMTKMEQFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHIDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FSEEKYKQVLIRM

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=434, Percent_Identity=23.7327188940092, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI40254478, Length=469, Percent_Identity=24.0938166311301, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21314632, Length=432, Percent_Identity=25, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI5032093, Length=413, Percent_Identity=27.3607748184019, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI66773030, Length=432, Percent_Identity=25.2314814814815, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI223468564, Length=274, Percent_Identity=29.1970802919708, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI223468566, Length=274, Percent_Identity=29.1970802919708, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI194239697, Length=448, Percent_Identity=23.8839285714286, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI4827012, Length=275, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=23.6702127659574, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI301601644, Length=175, Percent_Identity=27.4285714285714, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789947, Length=408, Percent_Identity=54.656862745098, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1790514, Length=415, Percent_Identity=40, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789473, Length=320, Percent_Identity=23.75, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=431, Percent_Identity=26.2180974477958, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71983099, Length=408, Percent_Identity=25.4901960784314, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71983106, Length=408, Percent_Identity=25.4901960784314, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=436, Percent_Identity=26.605504587156, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=424, Percent_Identity=24.7641509433962, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=425, Percent_Identity=24.9411764705882, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=408, Percent_Identity=24.5098039215686, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583025, Length=408, Percent_Identity=23.0392156862745, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17137668, Length=408, Percent_Identity=23.0392156862745, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583023, Length=408, Percent_Identity=23.0392156862745, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281360483, Length=422, Percent_Identity=24.6445497630332, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17137666, Length=422, Percent_Identity=24.6445497630332, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281360481, Length=422, Percent_Identity=24.6445497630332, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44919; Mature: 44919

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]