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The map label for this gene is rihA [H]

Identifier: 152975954

GI number: 152975954

Start: 2322320

End: 2323252

Strand: Reverse

Name: rihA [H]

Synonym: Bcer98_2226

Alternate gene names: 152975954

Gene position: 2323252-2322320 (Counterclockwise)

Preceding gene: 152975955

Following gene: 152975953

Centisome position: 56.84

GC content: 38.26

Gene sequence:

>933_bases
ATGAAGAAAGTACTGTTTTTGGGGGATCCAGGAATTGATGATTCGTTAGCGATTATGTATGGGTTGTTGCATCCTGATAT
TGATATTGTTGGTATTGTGACAGGATATGGGAATGTATCGCAAGAACAAGCGACAAGTAATGCGGCCTACTTATTACAAT
TAGCTGGGCGGGAAGATATTCCGGTTATTAAAGGAGCAATGATGCCACTGTCAGGAGAATTTACAACGTACTATCCAGAA
ATTCATGGTGCAGAAGGAATGGGGCCCATTCGTCCACCAAAAAGCATTTCACCGAATGTGAAACCTTTTTGTGAATTTTT
TTCTATTTTGGAAAAGTACAAAGGAGAAATTATTATTGTTGATGCTGGGAGATCAACAACATTAGCAACAGCTTTTATTT
TAGAAAAAACGCTTATGCAATATGTGAAAGAGTACTATATAATGGGCGGAGCCTTCTTAATACCAGGGAATGTTACGCCA
GTTGCTGAGGCAAATTTTTATGGAGACGCAATTGCTTCGCAACTTGTTATGCAAAATGCAAAAAACGTAACATTAATACC
GCTTAATGTTACCTCGGAAGCGATTATAACGCCAGAGACAGTAAAATATCTTGCAAAGCATTCAAAAACTGCTTTTCATA
CATTAATTGAACCGATTTTTACGTATTATTATAAGGCATATAAAAAGTTAAATCCGAAAATAACTGGAAGTCCGGTTCAT
GATGTTGTAACCATGATGGCGATGGCTAATCCCTCTTTTTTAGACTATGTATACCGCCGCGTTGCGGTGGATACAGATGG
AATTGCAAAGGGGGAAAGTATTGCGGACTTTCGACCACAGCCCGATGCGAAAGCGCTAAAAAATTGGATGAGAATTGGCT
GGTCGTTGCACTATAAAAAATTTCTTAAGGATTTCGTTAGAATTATGACATAG

Upstream 100 bases:

>100_bases
CAAAAAACACTGCGTTTTTTATGAGAAAATAAATTTTTTCATTCTGTAATTTTTTCCTGTTCATAAATTAATAACAACTG
TTAGGATGGAGTTGAAGTTT

Downstream 100 bases:

>100_bases
ACATAATAGCGACATACAAGTTAAAATAATAGAGACGACTTATTGATCAGTCGTCTCTATTATTTTGACTATGAATATAT
AAAATCGAGAAAAGACATAC

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGIVTGYGNVSQEQATSNAAYLLQLAGREDIPVIKGAMMPLSGEFTTYYPE
IHGAEGMGPIRPPKSISPNVKPFCEFFSILEKYKGEIIIVDAGRSTTLATAFILEKTLMQYVKEYYIMGGAFLIPGNVTP
VAEANFYGDAIASQLVMQNAKNVTLIPLNVTSEAIITPETVKYLAKHSKTAFHTLIEPIFTYYYKAYKKLNPKITGSPVH
DVVTMMAMANPSFLDYVYRRVAVDTDGIAKGESIADFRPQPDAKALKNWMRIGWSLHYKKFLKDFVRIMT

Sequences:

>Translated_310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGIVTGYGNVSQEQATSNAAYLLQLAGREDIPVIKGAMMPLSGEFTTYYPE
IHGAEGMGPIRPPKSISPNVKPFCEFFSILEKYKGEIIIVDAGRSTTLATAFILEKTLMQYVKEYYIMGGAFLIPGNVTP
VAEANFYGDAIASQLVMQNAKNVTLIPLNVTSEAIITPETVKYLAKHSKTAFHTLIEPIFTYYYKAYKKLNPKITGSPVH
DVVTMMAMANPSFLDYVYRRVAVDTDGIAKGESIADFRPQPDAKALKNWMRIGWSLHYKKFLKDFVRIMT
>Mature_310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGIVTGYGNVSQEQATSNAAYLLQLAGREDIPVIKGAMMPLSGEFTTYYPE
IHGAEGMGPIRPPKSISPNVKPFCEFFSILEKYKGEIIIVDAGRSTTLATAFILEKTLMQYVKEYYIMGGAFLIPGNVTP
VAEANFYGDAIASQLVMQNAKNVTLIPLNVTSEAIITPETVKYLAKHSKTAFHTLIEPIFTYYYKAYKKLNPKITGSPVH
DVVTMMAMANPSFLDYVYRRVAVDTDGIAKGESIADFRPQPDAKALKNWMRIGWSLHYKKFLKDFVRIMT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=277, Percent_Identity=29.9638989169675, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788486, Length=278, Percent_Identity=26.6187050359712, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786213, Length=284, Percent_Identity=28.5211267605634, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=195, Percent_Identity=29.2307692307692, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=252, Percent_Identity=33.7301587301587, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34444; Mature: 34444

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGIVTGYGNVSQEQATSNAAYLLQLAGREDI
CCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCEEEEEECCCCCC
PVIKGAMMPLSGEFTTYYPEIHGAEGMGPIRPPKSISPNVKPFCEFFSILEKYKGEIIIV
CCHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
DAGRSTTLATAFILEKTLMQYVKEYYIMGGAFLIPGNVTPVAEANFYGDAIASQLVMQNA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
KNVTLIPLNVTSEAIITPETVKYLAKHSKTAFHTLIEPIFTYYYKAYKKLNPKITGSPVH
CCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
DVVTMMAMANPSFLDYVYRRVAVDTDGIAKGESIADFRPQPDAKALKNWMRIGWSLHYKK
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLKDFVRIMT
HHHHHHHHCC
>Mature Secondary Structure
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGIVTGYGNVSQEQATSNAAYLLQLAGREDI
CCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCEEEEEECCCCCC
PVIKGAMMPLSGEFTTYYPEIHGAEGMGPIRPPKSISPNVKPFCEFFSILEKYKGEIIIV
CCHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
DAGRSTTLATAFILEKTLMQYVKEYYIMGGAFLIPGNVTPVAEANFYGDAIASQLVMQNA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
KNVTLIPLNVTSEAIITPETVKYLAKHSKTAFHTLIEPIFTYYYKAYKKLNPKITGSPVH
CCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
DVVTMMAMANPSFLDYVYRRVAVDTDGIAKGESIADFRPQPDAKALKNWMRIGWSLHYKK
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLKDFVRIMT
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA