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The map label for this gene is ykfB [H]

Identifier: 152975699

GI number: 152975699

Start: 2046585

End: 2047628

Strand: Reverse

Name: ykfB [H]

Synonym: Bcer98_1937

Alternate gene names: 152975699

Gene position: 2047628-2046585 (Counterclockwise)

Preceding gene: 152975700

Following gene: 152975698

Centisome position: 50.1

GC content: 39.37

Gene sequence:

>1044_bases
GTGGGAAATTTGAAAATTATAGATGTAAAGGTAAAGCGCCGATTGATTGAACTTCATACTCCGTTTAAAACAGCGCTGCG
CACTGTAACAGAGATTGAAAGTATTGACGTTTTTCTCCATATAGATAATGGAATGGTAGGAAGAGGAGCTGCTGCTCCAA
CTCCGGTTATTACAGGTGATTTTGCAAGTGGAATTGAAGAGGCGATTTTAGGACCGATTCGCTCTAGTTTAATAGGAAAA
GAGATTGAGCAACTTCATTCCTTATTATCATATGTTCAAAAGAGTTGTATTGGAAATACGAGCGCGAAGGCAGCAGTAGA
TATTGCTTTATATGATGCGTATTGTCAATTTCATAACATCCCGCTTTATGCCTTATTGGGTGGAAAGAAGGAATTTTATA
CAGATATTACGGTAAGTGTTGATGATCCTATATCGATGGCAAAAGAAGCGAAAAAGCATGTGGAAAAAGGATTTCAAACG
TTAAAGATTAAAGTTGGCCAATCCGCTCGGTTAGATTTAGAGCGAATTGAGGCCATTAAAAATATCATACCGAAGGATAT
TGCGCTCCGGTTGGATGCCAATCAAGGATGGGAACCGAAAGAGGCCGTTTCCATTATTAAAGAAATGGAAAAGAGAAATG
TAAATATCGAATTTATCGAACAGCCTGTTCATGCGCAAGATTGGGAAGGATTGAAATATGTCAAGAATCATGTATATATA
CCAATTATGGCTGATGAAAGTATTTTTTCAGCGGAAGATGCATTAAAGCTTGTTCAAGGACATTATGTTGATTGCATAAA
CATTAAATTAATGAAATGTGGCGGGATACGCCAGGCATGGAAAATTGCTGATGTCGCAGAAGCGGCAGGTGTGAAATGTA
TGATTGGCAGCATGATGGAATCATCGCTTTCTGTAACAGCCGCAGCGCATATAGCGGCTGCGCATCCGAATATTCATTAT
GTTGATCTTGATGCGCCGTTATGGCTAATGGAAGAGCCAGAAGGAATCAGTTATAGCGGAGCGAAGGTAAACTTACAGTT
TTAG

Upstream 100 bases:

>100_bases
TATAATCAGATTGCTGCTTTGTCTACTTCTTCATAGACAAAATCCAGCCGTAAGACGCTTTTCTTATGGCTGGATTTTTT
TATTGGACAAAGAGGAAAGG

Downstream 100 bases:

>100_bases
AAGAGTTATTGTTTCAAAGAATGATAAACTAGCAAATGATTTTTTTAAGGAGGATTTGTATGAAAAAAGTGGGGACTACA
TTATTAACAAGTCTATTTCT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MGNLKIIDVKVKRRLIELHTPFKTALRTVTEIESIDVFLHIDNGMVGRGAAAPTPVITGDFASGIEEAILGPIRSSLIGK
EIEQLHSLLSYVQKSCIGNTSAKAAVDIALYDAYCQFHNIPLYALLGGKKEFYTDITVSVDDPISMAKEAKKHVEKGFQT
LKIKVGQSARLDLERIEAIKNIIPKDIALRLDANQGWEPKEAVSIIKEMEKRNVNIEFIEQPVHAQDWEGLKYVKNHVYI
PIMADESIFSAEDALKLVQGHYVDCINIKLMKCGGIRQAWKIADVAEAAGVKCMIGSMMESSLSVTAAAHIAAAHPNIHY
VDLDAPLWLMEEPEGISYSGAKVNLQF

Sequences:

>Translated_347_residues
MGNLKIIDVKVKRRLIELHTPFKTALRTVTEIESIDVFLHIDNGMVGRGAAAPTPVITGDFASGIEEAILGPIRSSLIGK
EIEQLHSLLSYVQKSCIGNTSAKAAVDIALYDAYCQFHNIPLYALLGGKKEFYTDITVSVDDPISMAKEAKKHVEKGFQT
LKIKVGQSARLDLERIEAIKNIIPKDIALRLDANQGWEPKEAVSIIKEMEKRNVNIEFIEQPVHAQDWEGLKYVKNHVYI
PIMADESIFSAEDALKLVQGHYVDCINIKLMKCGGIRQAWKIADVAEAAGVKCMIGSMMESSLSVTAAAHIAAAHPNIHY
VDLDAPLWLMEEPEGISYSGAKVNLQF
>Mature_346_residues
GNLKIIDVKVKRRLIELHTPFKTALRTVTEIESIDVFLHIDNGMVGRGAAAPTPVITGDFASGIEEAILGPIRSSLIGKE
IEQLHSLLSYVQKSCIGNTSAKAAVDIALYDAYCQFHNIPLYALLGGKKEFYTDITVSVDDPISMAKEAKKHVEKGFQTL
KIKVGQSARLDLERIEAIKNIIPKDIALRLDANQGWEPKEAVSIIKEMEKRNVNIEFIEQPVHAQDWEGLKYVKNHVYIP
IMADESIFSAEDALKLVQGHYVDCINIKLMKCGGIRQAWKIADVAEAAGVKCMIGSMMESSLSVTAAAHIAAAHPNIHYV
DLDAPLWLMEEPEGISYSGAKVNLQF

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=324, Percent_Identity=30.2469135802469, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994953, Length=335, Percent_Identity=27.4626865671642, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 38307; Mature: 38176

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNLKIIDVKVKRRLIELHTPFKTALRTVTEIESIDVFLHIDNGMVGRGAAAPTPVITGD
CCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCEEECH
FASGIEEAILGPIRSSLIGKEIEQLHSLLSYVQKSCIGNTSAKAAVDIALYDAYCQFHNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHCCC
PLYALLGGKKEFYTDITVSVDDPISMAKEAKKHVEKGFQTLKIKVGQSARLDLERIEAIK
CEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
NIIPKDIALRLDANQGWEPKEAVSIIKEMEKRNVNIEFIEQPVHAQDWEGLKYVKNHVYI
HHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHCCEEE
PIMADESIFSAEDALKLVQGHYVDCINIKLMKCGGIRQAWKIADVAEAAGVKCMIGSMME
EEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
SSLSVTAAAHIAAAHPNIHYVDLDAPLWLMEEPEGISYSGAKVNLQF
HHHHHHHHHHHEECCCCEEEEECCCCEEEEECCCCCCCCCCEEEEEC
>Mature Secondary Structure 
GNLKIIDVKVKRRLIELHTPFKTALRTVTEIESIDVFLHIDNGMVGRGAAAPTPVITGD
CCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCEEECH
FASGIEEAILGPIRSSLIGKEIEQLHSLLSYVQKSCIGNTSAKAAVDIALYDAYCQFHNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHCCC
PLYALLGGKKEFYTDITVSVDDPISMAKEAKKHVEKGFQTLKIKVGQSARLDLERIEAIK
CEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
NIIPKDIALRLDANQGWEPKEAVSIIKEMEKRNVNIEFIEQPVHAQDWEGLKYVKNHVYI
HHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHCCEEE
PIMADESIFSAEDALKLVQGHYVDCINIKLMKCGGIRQAWKIADVAEAAGVKCMIGSMME
EEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
SSLSVTAAAHIAAAHPNIHYVDLDAPLWLMEEPEGISYSGAKVNLQF
HHHHHHHHHHHEECCCCEEEEECCCCEEEEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]