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The map label for this gene is ykfC [H]

Identifier: 152975698

GI number: 152975698

Start: 2045524

End: 2046525

Strand: Reverse

Name: ykfC [H]

Synonym: Bcer98_1936

Alternate gene names: 152975698

Gene position: 2046525-2045524 (Counterclockwise)

Preceding gene: 152975699

Following gene: 152975697

Centisome position: 50.07

GC content: 39.52

Gene sequence:

>1002_bases
ATGAAAAAAGTGGGGACTACATTATTAACAAGTCTATTTCTCTTTTCATCTTTCTCAGCAGTAAGTGCGAAAGAAAGAAT
AGGAAATACAGCTTTCATTGATGTTGCCGCTGCAACGCTATGGACTGGTCCGAATCTACTTCGTCCCATTGATGAGCCGA
GCGCAAGCAATCCTGTGGATCTATGGAAGTGGACGAAATCTATGACACTTGATGAAAAGCTTTGGTTAACAAATACAAAT
AAGCTGGAAACACAAGCGTTATTAGGGCAGGAAGTAACCGTTATTGAAAGGCAAGAAGAATGGGTGAAAGTCATTGTACA
TGGACAACCGACGCCAAGAAATGAACAAGGTTATCCAGGATGGATGCCAGAAAAACAACTAACGTATCACCAAGAATTTG
CGGATAAGAAAAATCAGTCCTTTGTTTTAATTACAAAACCTACAGCCATCTTATATATCAATCCTTCCGACAAAAACAAA
TCGTTAGAGGTCAGTTATAATACACGTTTGCCACTTGTAAGCGAAGATACCATTTCATATCGTGTTCTATTACCAAATGG
TCAAAAAGCTTGGTTACGTAAAAAAGATGGTACGGTATACCATTCACAAAATGATATACCGACGCCAACAGGGGACGATT
TAGTGAATACAGGTAAATTATTTTTAGGACTGCCATATATATGGGCTGGTACAAGTGGATTTGGATTTGATTGCTCTGGA
TTTACACATACGATTTACCAATCACATGGTATTACCATTCCGCGTGATTCAGGCCCACAGTCAAAAGCTGGCATGGCGGT
AGAAAAAGAACAGTTACAAAAAGGAGATTTAATTTTCTTTGCTCATGATGGTGGAAAAGGAAATGTTCATCATGTAGGAA
TGTACATTGGGGATGGAAAGATGATTCACTCTCCGAAAGCGGGAAGAACGGTTGAGATTATTCCGGTAGACACACCTGGA
TATATCGAAGAATATGCCGGAGCTCGGCGTTATTTACCATAA

Upstream 100 bases:

>100_bases
GAATCAGTTATAGCGGAGCGAAGGTAAACTTACAGTTTTAGAAGAGTTATTGTTTCAAAGAATGATAAACTAGCAAATGA
TTTTTTTAAGGAGGATTTGT

Downstream 100 bases:

>100_bases
TCAAAAATGATAGGAGGATGGAAATGAATCAGGTTATTCGTATTTCATTCATTAGTATGTTACTGGCTTCTTCCTTTTTA
GTTGGTTGTAGTGCAGAACA

Product: NLP/P60 protein

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKVGTTLLTSLFLFSSFSAVSAKERIGNTAFIDVAAATLWTGPNLLRPIDEPSASNPVDLWKWTKSMTLDEKLWLTNTN
KLETQALLGQEVTVIERQEEWVKVIVHGQPTPRNEQGYPGWMPEKQLTYHQEFADKKNQSFVLITKPTAILYINPSDKNK
SLEVSYNTRLPLVSEDTISYRVLLPNGQKAWLRKKDGTVYHSQNDIPTPTGDDLVNTGKLFLGLPYIWAGTSGFGFDCSG
FTHTIYQSHGITIPRDSGPQSKAGMAVEKEQLQKGDLIFFAHDGGKGNVHHVGMYIGDGKMIHSPKAGRTVEIIPVDTPG
YIEEYAGARRYLP

Sequences:

>Translated_333_residues
MKKVGTTLLTSLFLFSSFSAVSAKERIGNTAFIDVAAATLWTGPNLLRPIDEPSASNPVDLWKWTKSMTLDEKLWLTNTN
KLETQALLGQEVTVIERQEEWVKVIVHGQPTPRNEQGYPGWMPEKQLTYHQEFADKKNQSFVLITKPTAILYINPSDKNK
SLEVSYNTRLPLVSEDTISYRVLLPNGQKAWLRKKDGTVYHSQNDIPTPTGDDLVNTGKLFLGLPYIWAGTSGFGFDCSG
FTHTIYQSHGITIPRDSGPQSKAGMAVEKEQLQKGDLIFFAHDGGKGNVHHVGMYIGDGKMIHSPKAGRTVEIIPVDTPG
YIEEYAGARRYLP
>Mature_333_residues
MKKVGTTLLTSLFLFSSFSAVSAKERIGNTAFIDVAAATLWTGPNLLRPIDEPSASNPVDLWKWTKSMTLDEKLWLTNTN
KLETQALLGQEVTVIERQEEWVKVIVHGQPTPRNEQGYPGWMPEKQLTYHQEFADKKNQSFVLITKPTAILYINPSDKNK
SLEVSYNTRLPLVSEDTISYRVLLPNGQKAWLRKKDGTVYHSQNDIPTPTGDDLVNTGKLFLGLPYIWAGTSGFGFDCSG
FTHTIYQSHGITIPRDSGPQSKAGMAVEKEQLQKGDLIFFAHDGGKGNVHHVGMYIGDGKMIHSPKAGRTVEIIPVDTPG
YIEEYAGARRYLP

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=115, Percent_Identity=37.3913043478261, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786421, Length=115, Percent_Identity=36.5217391304348, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788501, Length=111, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 37097; Mature: 37097

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVGTTLLTSLFLFSSFSAVSAKERIGNTAFIDVAAATLWTGPNLLRPIDEPSASNPVD
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCHH
LWKWTKSMTLDEKLWLTNTNKLETQALLGQEVTVIERQEEWVKVIVHGQPTPRNEQGYPG
HHHHHHCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC
WMPEKQLTYHQEFADKKNQSFVLITKPTAILYINPSDKNKSLEVSYNTRLPLVSEDTISY
CCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEECCCEEE
RVLLPNGQKAWLRKKDGTVYHSQNDIPTPTGDDLVNTGKLFLGLPYIWAGTSGFGFDCSG
EEEECCCCEEEEECCCCEEEECCCCCCCCCCCCHHCCCEEEEECCEEEECCCCCCCCCCC
FTHTIYQSHGITIPRDSGPQSKAGMAVEKEQLQKGDLIFFAHDGGKGNVHHVGMYIGDGK
CEEEEECCCCEECCCCCCCCCCCCCEECHHHHCCCCEEEEEECCCCCCEEEEEEEEECCE
MIHSPKAGRTVEIIPVDTPGYIEEYAGARRYLP
EEECCCCCCEEEEEECCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKVGTTLLTSLFLFSSFSAVSAKERIGNTAFIDVAAATLWTGPNLLRPIDEPSASNPVD
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCHH
LWKWTKSMTLDEKLWLTNTNKLETQALLGQEVTVIERQEEWVKVIVHGQPTPRNEQGYPG
HHHHHHCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC
WMPEKQLTYHQEFADKKNQSFVLITKPTAILYINPSDKNKSLEVSYNTRLPLVSEDTISY
CCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEECCCEEE
RVLLPNGQKAWLRKKDGTVYHSQNDIPTPTGDDLVNTGKLFLGLPYIWAGTSGFGFDCSG
EEEECCCCEEEEECCCCEEEECCCCCCCCCCCCHHCCCEEEEECCEEEECCCCCCCCCCC
FTHTIYQSHGITIPRDSGPQSKAGMAVEKEQLQKGDLIFFAHDGGKGNVHHVGMYIGDGK
CEEEEECCCCEECCCCCCCCCCCCCEECHHHHCCCCEEEEEECCCCCCEEEEEEEEECCE
MIHSPKAGRTVEIIPVDTPGYIEEYAGARRYLP
EEECCCCCCEEEEEECCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]