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The map label for this gene is dppE [H]

Identifier: 152975697

GI number: 152975697

Start: 2043878

End: 2045500

Strand: Reverse

Name: dppE [H]

Synonym: Bcer98_1935

Alternate gene names: 152975697

Gene position: 2045500-2043878 (Counterclockwise)

Preceding gene: 152975698

Following gene: 152975696

Centisome position: 50.05

GC content: 32.9

Gene sequence:

>1623_bases
ATGAATCAGGTTATTCGTATTTCATTCATTAGTATGTTACTGGCTTCTTCCTTTTTAGTTGGTTGTAGTGCAGAACAATC
AACGACGAATTCGAAAGAAGAGAAGAAAGTATTACAATTAACGGAAACAGGAGAAATCCCATCATTGAACTCAGGAAAAG
TGACAGATGCGATTTCGTTTAACGTTTTAAACAATGTGATGGAAGGGCTATTTCGATTATCAAAAGATGATGAAGTCATT
GCAGGAATGGCTCAAAATTATGAAGTGAGTCAAGATGGTAAAAGATATACATTTCATTTGCGTGAAGCAGAATGGTCAAA
TGGAGACCTTGTCACAGCACATGATTTTGTTTATGCATGGAAACAAGTGATCAATCCAGAAACAGCTTCACAATATGCTT
ATATTATGTATGATGTGAAAAATGCAGAAAAGATCAATAAACAGCAATTAGGATTAGACGAACTAGGGGTAAAAGCAATA
GATAATAAAACTTTTGTTGTAGAATTAGAGCATCCTGTGCCGTACTTTACGAAGTTACTTTGTTTACCAACTTTTTATCC
AATTCATGAAAAATATGCAAAGGAACAAAGAGAGAAATACGGTTTAGAAGCTGATAAAACTGTATATAATGGCCCGTTCA
CATTATCGGAGTGGAAACATGAAGCAAGTTTCACTATGAAAAAGAACAATCAATATTGGGATAAAAAAGAGGTAAAACTA
GATGAAATCAACTATCAAATTGTAAAAGACATTTCAACTGTCGTGAATTTATATGAAACAAATAAAATTGATCGAGCTGT
TATTTCAACAGAATTTGTTGATAAATATAAGAACAATAAAGAACTGAAACAATTTACGGACCCTATTATGTATTTCTTTC
GTTTTAATGAAAGTGTACCGATTTTGAAAAATAAACATGCACGCCTAGCTTTAAGTATGGCTTTTGATAAAAAGGATCTT
GCTACATCTTTTTTAAATGACGGATCAGAACCTGCCAATTACTATATTCCAAGTGGCTTCTTATTAGATTCAGATAATAA
AGATTTTAGAGAAACGACGACAGAATTTAATAAAACAAATGTGAAACAGGCGAAAGAATATTGGGAAAAAGCAAAACAAG
AAACAGGAACAAATGAAGTTACGATTGAGATGTTAAACTACGATTTAGAAAACTTTAAAAAAGTTGGAGAGTACATTAAA
GAGCAGCTTGAAAAGAATCTTCCAGGTTTGACGATAAACGTAAAGTTACAACCTCATTCTCAAAAACTAGCATTAGAGAA
AAAGAAAGAATATGAAATGTCATTATCACGTTGGTTACCAGATTATCCAGATCCCATGACATATTTAGAGGTTTTTCTTT
CTGAAAATGGAGTGAATAATACGGGATATGCAAATCCAGAGTACGATGCATTACTGAAAAAGATAAAACTAGAGTTAGGA
AATGATGAACAGACACGCTGGAAAGCAATGCAAGATGCGGAAAAAATGTTGCTTGAAGATGCGGTCATTGCACCGGTATT
TCAGCACGGATTATCGTATTTACAAAAGCCATACGTTAAGAATTTATACGTACATCAATTTGGACCAGCAACAACTTTAA
AATGGACCGATATTAAAAAGTAA

Upstream 100 bases:

>100_bases
GAACGGTTGAGATTATTCCGGTAGACACACCTGGATATATCGAAGAATATGCCGGAGCTCGGCGTTATTTACCATAATCA
AAAATGATAGGAGGATGGAA

Downstream 100 bases:

>100_bases
AGGTTATGATTTAAAGAGCAGACTTCGTTTTATACGATAGTCTGTTTTTATTTATAGATATACTTTCTCATTGCAGTGAT
GAAGGAGTTAGTATGGTATA

Product: 4-phytase

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNQVIRISFISMLLASSFLVGCSAEQSTTNSKEEKKVLQLTETGEIPSLNSGKVTDAISFNVLNNVMEGLFRLSKDDEVI
AGMAQNYEVSQDGKRYTFHLREAEWSNGDLVTAHDFVYAWKQVINPETASQYAYIMYDVKNAEKINKQQLGLDELGVKAI
DNKTFVVELEHPVPYFTKLLCLPTFYPIHEKYAKEQREKYGLEADKTVYNGPFTLSEWKHEASFTMKKNNQYWDKKEVKL
DEINYQIVKDISTVVNLYETNKIDRAVISTEFVDKYKNNKELKQFTDPIMYFFRFNESVPILKNKHARLALSMAFDKKDL
ATSFLNDGSEPANYYIPSGFLLDSDNKDFRETTTEFNKTNVKQAKEYWEKAKQETGTNEVTIEMLNYDLENFKKVGEYIK
EQLEKNLPGLTINVKLQPHSQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSENGVNNTGYANPEYDALLKKIKLELG
NDEQTRWKAMQDAEKMLLEDAVIAPVFQHGLSYLQKPYVKNLYVHQFGPATTLKWTDIKK

Sequences:

>Translated_540_residues
MNQVIRISFISMLLASSFLVGCSAEQSTTNSKEEKKVLQLTETGEIPSLNSGKVTDAISFNVLNNVMEGLFRLSKDDEVI
AGMAQNYEVSQDGKRYTFHLREAEWSNGDLVTAHDFVYAWKQVINPETASQYAYIMYDVKNAEKINKQQLGLDELGVKAI
DNKTFVVELEHPVPYFTKLLCLPTFYPIHEKYAKEQREKYGLEADKTVYNGPFTLSEWKHEASFTMKKNNQYWDKKEVKL
DEINYQIVKDISTVVNLYETNKIDRAVISTEFVDKYKNNKELKQFTDPIMYFFRFNESVPILKNKHARLALSMAFDKKDL
ATSFLNDGSEPANYYIPSGFLLDSDNKDFRETTTEFNKTNVKQAKEYWEKAKQETGTNEVTIEMLNYDLENFKKVGEYIK
EQLEKNLPGLTINVKLQPHSQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSENGVNNTGYANPEYDALLKKIKLELG
NDEQTRWKAMQDAEKMLLEDAVIAPVFQHGLSYLQKPYVKNLYVHQFGPATTLKWTDIKK
>Mature_540_residues
MNQVIRISFISMLLASSFLVGCSAEQSTTNSKEEKKVLQLTETGEIPSLNSGKVTDAISFNVLNNVMEGLFRLSKDDEVI
AGMAQNYEVSQDGKRYTFHLREAEWSNGDLVTAHDFVYAWKQVINPETASQYAYIMYDVKNAEKINKQQLGLDELGVKAI
DNKTFVVELEHPVPYFTKLLCLPTFYPIHEKYAKEQREKYGLEADKTVYNGPFTLSEWKHEASFTMKKNNQYWDKKEVKL
DEINYQIVKDISTVVNLYETNKIDRAVISTEFVDKYKNNKELKQFTDPIMYFFRFNESVPILKNKHARLALSMAFDKKDL
ATSFLNDGSEPANYYIPSGFLLDSDNKDFRETTTEFNKTNVKQAKEYWEKAKQETGTNEVTIEMLNYDLENFKKVGEYIK
EQLEKNLPGLTINVKLQPHSQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSENGVNNTGYANPEYDALLKKIKLELG
NDEQTRWKAMQDAEKMLLEDAVIAPVFQHGLSYLQKPYVKNLYVHQFGPATTLKWTDIKK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=27.1535580524345, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87081878, Length=527, Percent_Identity=27.7039848197343, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789397, Length=491, Percent_Identity=27.2912423625255, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1787762, Length=523, Percent_Identity=21.0325047801147, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789966, Length=532, Percent_Identity=22.5563909774436, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787052, Length=339, Percent_Identity=23.3038348082596, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62570; Mature: 62570

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVIRISFISMLLASSFLVGCSAEQSTTNSKEEKKVLQLTETGEIPSLNSGKVTDAISF
CCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
NVLNNVMEGLFRLSKDDEVIAGMAQNYEVSQDGKRYTFHLREAEWSNGDLVTAHDFVYAW
HHHHHHHHHHHHCCCCCHHHHHHHHCCEECCCCCEEEEEEEECCCCCCCEEEHHHHHHHH
KQVINPETASQYAYIMYDVKNAEKINKQQLGLDELGVKAIDNKTFVVELEHPVPYFTKLL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHCCEEECCCEEEEEECCCCHHHHHHH
CLPTFYPIHEKYAKEQREKYGLEADKTVYNGPFTLSEWKHEASFTMKKNNQYWDKKEVKL
HHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCCEEEECCCCCCCCCCCCH
DEINYQIVKDISTVVNLYETNKIDRAVISTEFVDKYKNNKELKQFTDPIMYFFRFNESVP
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
ILKNKHARLALSMAFDKKDLATSFLNDGSEPANYYIPSGFLLDSDNKDFRETTTEFNKTN
CCCCCCCEEEEEEHHCHHHHHHHHHCCCCCCCCEECCCCEEECCCCCHHHHHHHHHHHCH
VKQAKEYWEKAKQETGTNEVTIEMLNYDLENFKKVGEYIKEQLEKNLPGLTINVKLQPHS
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
QKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSENGVNNTGYANPEYDALLKKIKLELG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
NDEQTRWKAMQDAEKMLLEDAVIAPVFQHGLSYLQKPYVKNLYVHQFGPATTLKWTDIKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEEEECCCCCEEEECCCCC
>Mature Secondary Structure
MNQVIRISFISMLLASSFLVGCSAEQSTTNSKEEKKVLQLTETGEIPSLNSGKVTDAISF
CCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
NVLNNVMEGLFRLSKDDEVIAGMAQNYEVSQDGKRYTFHLREAEWSNGDLVTAHDFVYAW
HHHHHHHHHHHHCCCCCHHHHHHHHCCEECCCCCEEEEEEEECCCCCCCEEEHHHHHHHH
KQVINPETASQYAYIMYDVKNAEKINKQQLGLDELGVKAIDNKTFVVELEHPVPYFTKLL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHCCEEECCCEEEEEECCCCHHHHHHH
CLPTFYPIHEKYAKEQREKYGLEADKTVYNGPFTLSEWKHEASFTMKKNNQYWDKKEVKL
HHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCCEEEECCCCCCCCCCCCH
DEINYQIVKDISTVVNLYETNKIDRAVISTEFVDKYKNNKELKQFTDPIMYFFRFNESVP
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
ILKNKHARLALSMAFDKKDLATSFLNDGSEPANYYIPSGFLLDSDNKDFRETTTEFNKTN
CCCCCCCEEEEEEHHCHHHHHHHHHCCCCCCCCEECCCCEEECCCCCHHHHHHHHHHHCH
VKQAKEYWEKAKQETGTNEVTIEMLNYDLENFKKVGEYIKEQLEKNLPGLTINVKLQPHS
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
QKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSENGVNNTGYANPEYDALLKKIKLELG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
NDEQTRWKAMQDAEKMLLEDAVIAPVFQHGLSYLQKPYVKNLYVHQFGPATTLKWTDIKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEEEECCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]