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The map label for this gene is opuAB [H]

Identifier: 152975672

GI number: 152975672

Start: 2020398

End: 2021237

Strand: Reverse

Name: opuAB [H]

Synonym: Bcer98_1907

Alternate gene names: 152975672

Gene position: 2021237-2020398 (Counterclockwise)

Preceding gene: 152975673

Following gene: 152975671

Centisome position: 49.45

GC content: 37.14

Gene sequence:

>840_bases
ATGAATAATATTCCGCGTATTCCGTTAGGTGAATGGGTCGATACGTTTGTAGCGAGTTTATATGAGCATTTTGAAAATAT
ATTTAGAGGATTTTCTTATGTGATTGGTGGTTTTGTTGATTTACTGACAAATTTTTTAACATTCATTCCAGCTTTTTTAA
TGATTGTCATTGTTTGCTTTCTCATTTGGTATACAGCAAGGAAAGTCTCTTTACTTATTTTTACATTGATTGGCTTATTA
TTTATTTTAAACATTAACTATTGGGAACAAACGATGCAAACATTGGCGCTTGTTCTGACATCCGTTATTATTTCTATCAT
TGTTGGAATCCCTATTGGCATTTTAGCATCGCAAAACGAACGGTTTTCGAAATTTTTAAAACCAACATTAGATTTTATGC
AAACAATGCCGGCATTTGTGTATTTAATTCCTGCCATTACATTCTTTGGAGTTGGGGTAGTGCCAGGGATTATCGCGTCG
GTTATTTTTGCGATGCCACCAACGATTCGCTTTACAGACCTTGGAATTAGACAAGTACCGGAAGATTTAATTGAAGCTGC
AGATGCATTTGGATCTACAGCATCACAAAAGCTATTTAAAGTACAATTGCCACTTGCGACAGGAACGATTATGGCCGGGA
TTAATCAGAGTATTATGCTATCATTGTCGATGGTTGTGACTGCATCACTTGTAGGAGCGCCGGGACTTGGTGTAGATGTA
TATCGCTCTGTAACACAAGTGAATATTGGAATGGGGTTTGAAGCTGGGTTAGCCATTGTCGTAATCGCTATTATATTGGA
TCGAATCACACAAGGGTTTCATCGAAGAAAAAGAGGGTAG

Upstream 100 bases:

>100_bases
TTACCTGTTATTGATGAAAAAAATAGGCTAAGGGGGATTATTAAACGAGAAAGTGTCATTCAAGCCCTTGCCGGAAATAT
TGAAGATGAGGTGAATGACA

Downstream 100 bases:

>100_bases
GACAGAACAGTTTCAGTAAAATAATCAAAAAAGATATTTTTCAAGAAGTTACAATGAATTTTTTGTGAAAAATTCATTGT
AACTTTAAATAAACTTCATA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNNIPRIPLGEWVDTFVASLYEHFENIFRGFSYVIGGFVDLLTNFLTFIPAFLMIVIVCFLIWYTARKVSLLIFTLIGLL
FILNINYWEQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAADAFGSTASQKLFKVQLPLATGTIMAGINQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHRRKRG

Sequences:

>Translated_279_residues
MNNIPRIPLGEWVDTFVASLYEHFENIFRGFSYVIGGFVDLLTNFLTFIPAFLMIVIVCFLIWYTARKVSLLIFTLIGLL
FILNINYWEQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAADAFGSTASQKLFKVQLPLATGTIMAGINQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHRRKRG
>Mature_279_residues
MNNIPRIPLGEWVDTFVASLYEHFENIFRGFSYVIGGFVDLLTNFLTFIPAFLMIVIVCFLIWYTARKVSLLIFTLIGLL
FILNINYWEQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAADAFGSTASQKLFKVQLPLATGTIMAGINQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHRRKRG

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=279, Percent_Identity=45.8781362007168, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1788451, Length=197, Percent_Identity=26.3959390862944, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788449, Length=190, Percent_Identity=27.8947368421053, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30705; Mature: 30705

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIPRIPLGEWVDTFVASLYEHFENIFRGFSYVIGGFVDLLTNFLTFIPAFLMIVIVCF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIWYTARKVSLLIFTLIGLLFILNINYWEQTMQTLALVLTSVIISIIVGIPIGILASQNE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCH
RFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIASVIFAMPPTIRFTDLGIRQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCC
EDLIEAADAFGSTASQKLFKVQLPLATGTIMAGINQSIMLSLSMVVTASLVGAPGLGVDV
HHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHRRKRG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNIPRIPLGEWVDTFVASLYEHFENIFRGFSYVIGGFVDLLTNFLTFIPAFLMIVIVCF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIWYTARKVSLLIFTLIGLLFILNINYWEQTMQTLALVLTSVIISIIVGIPIGILASQNE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCH
RFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIASVIFAMPPTIRFTDLGIRQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCC
EDLIEAADAFGSTASQKLFKVQLPLATGTIMAGINQSIMLSLSMVVTASLVGAPGLGVDV
HHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHRRKRG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]