Click here to switch to the map view.

The map label for this gene is thiF [C]

Identifier: 152975249

GI number: 152975249

Start: 1557755

End: 1560295

Strand: Direct

Name: thiF [C]

Synonym: Bcer98_1454

Alternate gene names: 152975249

Gene position: 1557755-1560295 (Clockwise)

Preceding gene: 152975248

Following gene: 152975250

Centisome position: 38.11

GC content: 30.93

Gene sequence:

>2541_bases
ATGAGATTAAAAACAAGTTTGAATTTTAGAGGGTATCCAAATAGAAATGAAATTGTTTATTACGATGGCGAAGAACGAGT
GATTGAAGTGAATGAATTTGAAAAGCTATGGTCATTACTGAAAATTTTGGAAAGCCCGAAAGAAGATGTTCATACAAAAA
TACAGGAATGGAAAAATAACAACGACATAGAGGATGAGGAGTTACATCAAATTCTCCAATTTATAAATGAGAATGGAATG
TTATATGAAAAGCGTTGTGACAAGATGGATGAGGAACAATTATATAATATAAGAAACTTTCATTATTTCAGTACACACGA
TTCTACTATATATGCAGATGCGATTGTTCAAAGGATAAAAAAGGTAAAAGCAGTTGTAATCGGGGCTGGAACGATAGGTG
CAACATTATGTATGACATTATCTAAACTTGGTGTCGGAGAGATTATTGTTATCGATTTTGATACGGTACAACTAAAAAAT
ATTAGAGCGCAGACAATATTTCAAAAAGAAGATACGAATAAGAAAAAAATACATGTTATCCAAGAAAAGTTAAAGAAAAT
GGATCCGTATGTTAAAGTTCAAGTGTATGATATGAAAATCGAAACAATCCATGATTTGTTACGAGTAGACTTACATGATG
TGCATTATATTTTTGGATGTTTTGATGAATCTTCACTTCAATTGCAAAAAGATATAATGAATTATTGTGATAAGGAAAAG
ATTCAATATTATTTGATGGGTTACCATAATGATTTTGTCAAAGTGTTTCATGTAAGCAATCGGAATGATGGAGAGAGACT
GCTTGAAGAGAGTTTTCAAAACTATCATACAGAATATGTTATTCGAGAAAATAGGGGGACGATCATTCAAAGCCTAGCTG
TTTCGCTTATAATTAGTCGGATTCTATTCGAGGACATAACAAAAAGCAGTTGTACAGTGCCAAGTGGGTATCATTTTGAC
TTTATTACATTTCAAACTTCTCATAACCGTCAATCTATCTCGAGAGAACCATTCGTTCAATCATTGCAAAGAATTATGCC
CTTTGACCAAGAACAATTAAATCGGAAGATAGAATTCCTTTTCAATATTATTGATAAAAAAGAGAAAGTGACGATTCTTC
CGAAAGTAATAGAAATGGATATTTTATCTATGCACCAAGTGTTTGACATTTTATTTCATATAGGTCAAATTGCTAGCTTA
CAGTTAGAAGATCACTACAATAAATTTATAGAACTGATGAATGAAATAGACAAAACAGAAGACCCAGAACATAATGAATA
TGAACAATATCTACAATTCATTCGTAGCATGAAAATAAATTATGAAGATGAGGTATACACCATATTTGAAATTTTTGAAA
TGATTCGCAACACGAAGGATTATGAGGAAAAGAAAAAAATGCAAAGCGGAATCTATGAAGTATTGAAACAGAATGGTGAC
ACACTGCTTTCGTTTTTTGTAAACAGTAAGAAGAAGTATTTAGCTTTAGAAATCCCGAATTATTACATGGAAGTCTTTGG
CGTTAAAGAGGAAACGTTACATATACTCGAAAATGAGTTGCAAAAGAAGTTCCATACATTACTTACGAAAAGTCTGTCTA
TGATGTTTTCAAATTCATTTCATGAAATTGGCGTAGACTTTTTATCATATAATGAAGAAGAACACAGTATGATTACACTG
GATGAGGCAAAACATTTCATCGTTACAAGTTTAGAGAAAGATGGGAAGCATCATTTTGTTCATTATATAGAGCGAATGTT
CGAGGAGAATTTTATACAAGTATATAATAATGTTGAAGTGAATAAAACATACTATTTTCCAAGTATGAAGGAGAGTAGAA
TTGTATTTAACTATCATAATGATATGGATAGTGTATTTGTGCTATGCCATGAATTAGGACATGCTTATTTTAACCAAAGT
TATGGACATACATTTTTTGACGATAGTACACAATTAGTTAACGAAATGATGGCATACTATTTTGAAATTATTTGCATTCA
ATCAATGCTAGGAAATGAAGAGATAAAGATTGAAATGAAACAAGAAATAGCACGACAATATATAAAAAGAATACATCAAA
CTGTCTTATCCACATACGGAGTTCACTTGTTAGAAAAAAGCTTAGTTAAGCATATAGAGGAACACGGAACCATTTCGTTA
CTCGACTTTTTGAAAATTCGTGATGAATATAATCAACATTCTTTTTTTAAAGGGATCAAATTTAAAAATGAAAAGTATTT
TTATTTAAATCCACTTTTGAAATCTTCATTTATGCTTGAATTTGGAGAGCATCTTTTACCCCCAATGGCATATTTACTTG
CGGTTAGTTTATACAATGACCGAAGTGAAACAAGTATTCCAAAAGATATTCGAATGCAAGAGGCTATATATAATGGGGTA
TATTGTACAGAAGAGTTTTTATCTTATGTAGCAAAGGATGTTCCTCATGATGAACGTATGAAACAAGCAATTCATACACT
TTTAGAACTGTTTTGTAAACTGGAATCATTTACGATGAAGGATGAAGTATATTCCAATTAA

Upstream 100 bases:

>100_bases
CTACGCAACAAGATAATCAATGTGTATGTTTATTATTATGCTAGTTTGAAAAAAGAAGGTGTGCAAGCACCTTCTTTTTC
AATGGAATGGGGAGAAAAAG

Downstream 100 bases:

>100_bases
AAATAAAAAAGTTATGAATAGAGACATGTCTCCATTCATAACTTTTTTATTTTGTTTCGAAATTTAACGAGAAAAGAAGA
CTTATGTAAGAGGAGCATAT

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: None

Number of amino acids: Translated: 846; Mature: 846

Protein sequence:

>846_residues
MRLKTSLNFRGYPNRNEIVYYDGEERVIEVNEFEKLWSLLKILESPKEDVHTKIQEWKNNNDIEDEELHQILQFINENGM
LYEKRCDKMDEEQLYNIRNFHYFSTHDSTIYADAIVQRIKKVKAVVIGAGTIGATLCMTLSKLGVGEIIVIDFDTVQLKN
IRAQTIFQKEDTNKKKIHVIQEKLKKMDPYVKVQVYDMKIETIHDLLRVDLHDVHYIFGCFDESSLQLQKDIMNYCDKEK
IQYYLMGYHNDFVKVFHVSNRNDGERLLEESFQNYHTEYVIRENRGTIIQSLAVSLIISRILFEDITKSSCTVPSGYHFD
FITFQTSHNRQSISREPFVQSLQRIMPFDQEQLNRKIEFLFNIIDKKEKVTILPKVIEMDILSMHQVFDILFHIGQIASL
QLEDHYNKFIELMNEIDKTEDPEHNEYEQYLQFIRSMKINYEDEVYTIFEIFEMIRNTKDYEEKKKMQSGIYEVLKQNGD
TLLSFFVNSKKKYLALEIPNYYMEVFGVKEETLHILENELQKKFHTLLTKSLSMMFSNSFHEIGVDFLSYNEEEHSMITL
DEAKHFIVTSLEKDGKHHFVHYIERMFEENFIQVYNNVEVNKTYYFPSMKESRIVFNYHNDMDSVFVLCHELGHAYFNQS
YGHTFFDDSTQLVNEMMAYYFEIICIQSMLGNEEIKIEMKQEIARQYIKRIHQTVLSTYGVHLLEKSLVKHIEEHGTISL
LDFLKIRDEYNQHSFFKGIKFKNEKYFYLNPLLKSSFMLEFGEHLLPPMAYLLAVSLYNDRSETSIPKDIRMQEAIYNGV
YCTEEFLSYVAKDVPHDERMKQAIHTLLELFCKLESFTMKDEVYSN

Sequences:

>Translated_846_residues
MRLKTSLNFRGYPNRNEIVYYDGEERVIEVNEFEKLWSLLKILESPKEDVHTKIQEWKNNNDIEDEELHQILQFINENGM
LYEKRCDKMDEEQLYNIRNFHYFSTHDSTIYADAIVQRIKKVKAVVIGAGTIGATLCMTLSKLGVGEIIVIDFDTVQLKN
IRAQTIFQKEDTNKKKIHVIQEKLKKMDPYVKVQVYDMKIETIHDLLRVDLHDVHYIFGCFDESSLQLQKDIMNYCDKEK
IQYYLMGYHNDFVKVFHVSNRNDGERLLEESFQNYHTEYVIRENRGTIIQSLAVSLIISRILFEDITKSSCTVPSGYHFD
FITFQTSHNRQSISREPFVQSLQRIMPFDQEQLNRKIEFLFNIIDKKEKVTILPKVIEMDILSMHQVFDILFHIGQIASL
QLEDHYNKFIELMNEIDKTEDPEHNEYEQYLQFIRSMKINYEDEVYTIFEIFEMIRNTKDYEEKKKMQSGIYEVLKQNGD
TLLSFFVNSKKKYLALEIPNYYMEVFGVKEETLHILENELQKKFHTLLTKSLSMMFSNSFHEIGVDFLSYNEEEHSMITL
DEAKHFIVTSLEKDGKHHFVHYIERMFEENFIQVYNNVEVNKTYYFPSMKESRIVFNYHNDMDSVFVLCHELGHAYFNQS
YGHTFFDDSTQLVNEMMAYYFEIICIQSMLGNEEIKIEMKQEIARQYIKRIHQTVLSTYGVHLLEKSLVKHIEEHGTISL
LDFLKIRDEYNQHSFFKGIKFKNEKYFYLNPLLKSSFMLEFGEHLLPPMAYLLAVSLYNDRSETSIPKDIRMQEAIYNGV
YCTEEFLSYVAKDVPHDERMKQAIHTLLELFCKLESFTMKDEVYSN
>Mature_846_residues
MRLKTSLNFRGYPNRNEIVYYDGEERVIEVNEFEKLWSLLKILESPKEDVHTKIQEWKNNNDIEDEELHQILQFINENGM
LYEKRCDKMDEEQLYNIRNFHYFSTHDSTIYADAIVQRIKKVKAVVIGAGTIGATLCMTLSKLGVGEIIVIDFDTVQLKN
IRAQTIFQKEDTNKKKIHVIQEKLKKMDPYVKVQVYDMKIETIHDLLRVDLHDVHYIFGCFDESSLQLQKDIMNYCDKEK
IQYYLMGYHNDFVKVFHVSNRNDGERLLEESFQNYHTEYVIRENRGTIIQSLAVSLIISRILFEDITKSSCTVPSGYHFD
FITFQTSHNRQSISREPFVQSLQRIMPFDQEQLNRKIEFLFNIIDKKEKVTILPKVIEMDILSMHQVFDILFHIGQIASL
QLEDHYNKFIELMNEIDKTEDPEHNEYEQYLQFIRSMKINYEDEVYTIFEIFEMIRNTKDYEEKKKMQSGIYEVLKQNGD
TLLSFFVNSKKKYLALEIPNYYMEVFGVKEETLHILENELQKKFHTLLTKSLSMMFSNSFHEIGVDFLSYNEEEHSMITL
DEAKHFIVTSLEKDGKHHFVHYIERMFEENFIQVYNNVEVNKTYYFPSMKESRIVFNYHNDMDSVFVLCHELGHAYFNQS
YGHTFFDDSTQLVNEMMAYYFEIICIQSMLGNEEIKIEMKQEIARQYIKRIHQTVLSTYGVHLLEKSLVKHIEEHGTISL
LDFLKIRDEYNQHSFFKGIKFKNEKYFYLNPLLKSSFMLEFGEHLLPPMAYLLAVSLYNDRSETSIPKDIRMQEAIYNGV
YCTEEFLSYVAKDVPHDERMKQAIHTLLELFCKLESFTMKDEVYSN

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG1179

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 100820; Mature: 100820

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKTSLNFRGYPNRNEIVYYDGEERVIEVNEFEKLWSLLKILESPKEDVHTKIQEWKNN
CCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
NDIEDEELHQILQFINENGMLYEKRCDKMDEEQLYNIRNFHYFSTHDSTIYADAIVQRIK
CCCCHHHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHCEEEEECCCCHHHHHHHHHHHH
KVKAVVIGAGTIGATLCMTLSKLGVGEIIVIDFDTVQLKNIRAQTIFQKEDTNKKKIHVI
HHHHEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHHHEECCCCCCHHHHHH
QEKLKKMDPYVKVQVYDMKIETIHDLLRVDLHDVHYIFGCFDESSLQLQKDIMNYCDKEK
HHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCHHH
IQYYLMGYHNDFVKVFHVSNRNDGERLLEESFQNYHTEYVIRENRGTIIQSLAVSLIISR
EEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
ILFEDITKSSCTVPSGYHFDFITFQTSHNRQSISREPFVQSLQRIMPFDQEQLNRKIEFL
HHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHH
FNIIDKKEKVTILPKVIEMDILSMHQVFDILFHIGQIASLQLEDHYNKFIELMNEIDKTE
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCC
DPEHNEYEQYLQFIRSMKINYEDEVYTIFEIFEMIRNTKDYEEKKKMQSGIYEVLKQNGD
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCH
TLLSFFVNSKKKYLALEIPNYYMEVFGVKEETLHILENELQKKFHTLLTKSLSMMFSNSF
HHHHHHHCCCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
HEIGVDFLSYNEEEHSMITLDEAKHFIVTSLEKDGKHHFVHYIERMFEENFIQVYNNVEV
HHHCCHHHHCCCCCCCEEEHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
NKTYYFPSMKESRIVFNYHNDMDSVFVLCHELGHAYFNQSYGHTFFDDSTQLVNEMMAYY
CEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FEIICIQSMLGNEEIKIEMKQEIARQYIKRIHQTVLSTYGVHLLEKSLVKHIEEHGTISL
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LDFLKIRDEYNQHSFFKGIKFKNEKYFYLNPLLKSSFMLEFGEHLLPPMAYLLAVSLYND
HHHHHHHHHCCHHHHHHCCEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSETSIPKDIRMQEAIYNGVYCTEEFLSYVAKDVPHDERMKQAIHTLLELFCKLESFTMK
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DEVYSN
HHHCCC
>Mature Secondary Structure
MRLKTSLNFRGYPNRNEIVYYDGEERVIEVNEFEKLWSLLKILESPKEDVHTKIQEWKNN
CCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
NDIEDEELHQILQFINENGMLYEKRCDKMDEEQLYNIRNFHYFSTHDSTIYADAIVQRIK
CCCCHHHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHCEEEEECCCCHHHHHHHHHHHH
KVKAVVIGAGTIGATLCMTLSKLGVGEIIVIDFDTVQLKNIRAQTIFQKEDTNKKKIHVI
HHHHEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHHHEECCCCCCHHHHHH
QEKLKKMDPYVKVQVYDMKIETIHDLLRVDLHDVHYIFGCFDESSLQLQKDIMNYCDKEK
HHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCHHH
IQYYLMGYHNDFVKVFHVSNRNDGERLLEESFQNYHTEYVIRENRGTIIQSLAVSLIISR
EEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
ILFEDITKSSCTVPSGYHFDFITFQTSHNRQSISREPFVQSLQRIMPFDQEQLNRKIEFL
HHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHCCCCHHHHHHHHHCCCCHHHHHHHHHHH
FNIIDKKEKVTILPKVIEMDILSMHQVFDILFHIGQIASLQLEDHYNKFIELMNEIDKTE
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCC
DPEHNEYEQYLQFIRSMKINYEDEVYTIFEIFEMIRNTKDYEEKKKMQSGIYEVLKQNGD
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCH
TLLSFFVNSKKKYLALEIPNYYMEVFGVKEETLHILENELQKKFHTLLTKSLSMMFSNSF
HHHHHHHCCCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
HEIGVDFLSYNEEEHSMITLDEAKHFIVTSLEKDGKHHFVHYIERMFEENFIQVYNNVEV
HHHCCHHHHCCCCCCCEEEHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
NKTYYFPSMKESRIVFNYHNDMDSVFVLCHELGHAYFNQSYGHTFFDDSTQLVNEMMAYY
CEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FEIICIQSMLGNEEIKIEMKQEIARQYIKRIHQTVLSTYGVHLLEKSLVKHIEEHGTISL
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LDFLKIRDEYNQHSFFKGIKFKNEKYFYLNPLLKSSFMLEFGEHLLPPMAYLLAVSLYND
HHHHHHHHHCCHHHHHHCCEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSETSIPKDIRMQEAIYNGVYCTEEFLSYVAKDVPHDERMKQAIHTLLELFCKLESFTMK
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DEVYSN
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA