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The map label for this gene is panB

Identifier: 152975062

GI number: 152975062

Start: 1369777

End: 1370616

Strand: Direct

Name: panB

Synonym: Bcer98_1262

Alternate gene names: 152975062

Gene position: 1369777-1370616 (Clockwise)

Preceding gene: 152975061

Following gene: 152975063

Centisome position: 33.52

GC content: 40.36

Gene sequence:

>840_bases
TTGAAAACGAAAACAGATTTTTTGAGAATGAAACAGAAAGGTGAGCCGATTACGATGCTGACAGCGTATGACTACCCCTC
AGCTAAGTTAGTAGAGGAAGCTGAAGTCGATATGATTTTAGTTGGAGATTCTCTCGGAATGGTTGTACTTGGATATGATT
CCACAGTACCTGTGACGGTAGAGGATATGATTCATCATACAAAAGCTGTCCGCCGAGGGGCGAAGGATACGTTTGTTGTA
ACAGATATGCCATTTATGTCTTATCATGTATCGCTGGCAGAAACTATGCATCATGCGCGTCGCATTGTGCAAGAAGGCGG
AGCGCATGCTTTAAAAGTAGAAGGAGCTGGAGAAGTCATTTCCACTATTCATTATTTAACGAATGCAGGAATTCCTGTTG
TTGCTCATCTTGGATTAACGCCACAGTCTGTCGGTATATTAGGTGGTTATAAAGTACAAGGGAAAGATGCTGAAAGTGCA
AAACAATTAATAGCAGATGCGAAGAAATGTGAAGATGCTGGAGCAATAGCTCTCGTATTAGAATGTGTACCGATGCAGTT
AGCAAAGATAATATCAGATGAGTTAACTATTCCGACAATTGGCATCGGTGCTGGTAAAGATGTGGATGGGCAAGTGCTAG
TCTATCATGATTTGATTTCTTACGGAGTAAATAGAGTTCCGAAATTTGTCAAGCAATATACGGCTGTTCAGGATGAAATT
GTGCGTGGGATTTCGCAATATGTGGCTGAAGTAAAAACAAGACAATTTCCAGAAGATCAATATTCATTTACGATGAAAGA
AGAAGAGTATGTAGCTTTATATGGAGGAAAACAGGGATGA

Upstream 100 bases:

>100_bases
GCCGATTTACAAACACTACCCTTCTGCCCTTTTATGGTCAGAAGGTTTTTTTATATCATTTCGGCCATCGCCTCTCTCCT
AAATAAAGGAGGAGTAATAT

Downstream 100 bases:

>100_bases
AAGTGATAACAACAGTGAAGGACATGCAACAAATTGCAGGCGAACTTCGGGCAAGTGGTAAAGAGATTGGGTTTGTTCCA
ACAATGGGATATTTACATGA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKTKTDFLRMKQKGEPITMLTAYDYPSAKLVEEAEVDMILVGDSLGMVVLGYDSTVPVTVEDMIHHTKAVRRGAKDTFVV
TDMPFMSYHVSLAETMHHARRIVQEGGAHALKVEGAGEVISTIHYLTNAGIPVVAHLGLTPQSVGILGGYKVQGKDAESA
KQLIADAKKCEDAGAIALVLECVPMQLAKIISDELTIPTIGIGAGKDVDGQVLVYHDLISYGVNRVPKFVKQYTAVQDEI
VRGISQYVAEVKTRQFPEDQYSFTMKEEEYVALYGGKQG

Sequences:

>Translated_279_residues
MKTKTDFLRMKQKGEPITMLTAYDYPSAKLVEEAEVDMILVGDSLGMVVLGYDSTVPVTVEDMIHHTKAVRRGAKDTFVV
TDMPFMSYHVSLAETMHHARRIVQEGGAHALKVEGAGEVISTIHYLTNAGIPVVAHLGLTPQSVGILGGYKVQGKDAESA
KQLIADAKKCEDAGAIALVLECVPMQLAKIISDELTIPTIGIGAGKDVDGQVLVYHDLISYGVNRVPKFVKQYTAVQDEI
VRGISQYVAEVKTRQFPEDQYSFTMKEEEYVALYGGKQG
>Mature_279_residues
MKTKTDFLRMKQKGEPITMLTAYDYPSAKLVEEAEVDMILVGDSLGMVVLGYDSTVPVTVEDMIHHTKAVRRGAKDTFVV
TDMPFMSYHVSLAETMHHARRIVQEGGAHALKVEGAGEVISTIHYLTNAGIPVVAHLGLTPQSVGILGGYKVQGKDAESA
KQLIADAKKCEDAGAIALVLECVPMQLAKIISDELTIPTIGIGAGKDVDGQVLVYHDLISYGVNRVPKFVKQYTAVQDEI
VRGISQYVAEVKTRQFPEDQYSFTMKEEEYVALYGGKQG

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=255, Percent_Identity=47.0588235294118, Blast_Score=239, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6319653, Length=265, Percent_Identity=39.622641509434, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BACCN (A7GN76)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374579.1
- ProteinModelPortal:   A7GN76
- SMR:   A7GN76
- STRING:   A7GN76
- EnsemblBacteria:   EBBACT00000038497
- GeneID:   5346020
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1262
- eggNOG:   COG0413
- GeneTree:   EBGT00050000000505
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   BCER315749:BCER98_1262-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30464; Mature: 30464

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 82-82 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKTDFLRMKQKGEPITMLTAYDYPSAKLVEEAEVDMILVGDSLGMVVLGYDSTVPVTV
CCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEH
EDMIHHTKAVRRGAKDTFVVTDMPFMSYHVSLAETMHHARRIVQEGGAHALKVEGAGEVI
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
STIHYLTNAGIPVVAHLGLTPQSVGILGGYKVQGKDAESAKQLIADAKKCEDAGAIALVL
HHHHHHHCCCCCEEEECCCCCCCCCEECCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHH
ECVPMQLAKIISDELTIPTIGIGAGKDVDGQVLVYHDLISYGVNRVPKFVKQYTAVQDEI
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VRGISQYVAEVKTRQFPEDQYSFTMKEEEYVALYGGKQG
HHHHHHHHHHHHHHCCCCCHHEEEECCCCEEEEECCCCC
>Mature Secondary Structure
MKTKTDFLRMKQKGEPITMLTAYDYPSAKLVEEAEVDMILVGDSLGMVVLGYDSTVPVTV
CCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEH
EDMIHHTKAVRRGAKDTFVVTDMPFMSYHVSLAETMHHARRIVQEGGAHALKVEGAGEVI
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
STIHYLTNAGIPVVAHLGLTPQSVGILGGYKVQGKDAESAKQLIADAKKCEDAGAIALVL
HHHHHHHCCCCCEEEECCCCCCCCCEECCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHH
ECVPMQLAKIISDELTIPTIGIGAGKDVDGQVLVYHDLISYGVNRVPKFVKQYTAVQDEI
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VRGISQYVAEVKTRQFPEDQYSFTMKEEEYVALYGGKQG
HHHHHHHHHHHHHHCCCCCHHEEEECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA