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The map label for this gene is birA [H]

Identifier: 152975061

GI number: 152975061

Start: 1368515

End: 1369495

Strand: Direct

Name: birA [H]

Synonym: Bcer98_1261

Alternate gene names: 152975061

Gene position: 1368515-1369495 (Clockwise)

Preceding gene: 152975060

Following gene: 152975062

Centisome position: 33.48

GC content: 39.14

Gene sequence:

>981_bases
ATGCAATCTACTATGAGAAAACAATTGTTACAAATTTTTTCTGAGGCAAATGGAGAATTTGTATCTGGACAAACGATAAG
TGAAAAGCTCGGTTGTTCTAGAACGGCTGTGTGGAAGCATATGGAGGATTTGCGCACAGAAGGTTATGAATTGGAGGCGA
TTCGCCGCTTAGGATACCGAATTACGAGTAAGCCAGATAAAGTGACAGCAAATGAAATTCAATTAGGGCTACAAACAGAC
TTTATAGGGAGAACGGTATACTTTGAAGAGTCGGTTGAATCTACGCAGCATGTTGCAGCGAAACTTGCATATGAAGGTGC
AGCGGAAGGAACAATAGTCGTCGCCGAAGAACAAACAGCAGGGAGAGGACGCTTAAGTCGAAAATGGTATTCGCCAAAAG
GAACAGGAGTTTGGATGAGCATTATTTTACGCCCATCCATTCCTGTTCATCATGCGCCTCAGCTTACTTTACTAGCTGCT
GTTAGCGTGGCGCAAGCAATCGAGAAATGTACGGGCGTGAATGTAGGGATTAAATGGCCGAATGATATTTTAATAAATGG
AAAAAAAACTGTAGGTATTTTAACAGAAATGCAAGCTGATCCAGATAAAATTAATGCAGTTATTATGGGGATTGGAATTA
ATGCAAATCAACAAACAGAACATTTTGTTGATGAGATTAAACAAATTGCTACCTCTTTAGCCATCGAGTCAGGAAAACCA
ATTGTTCGTGCAGAACTTATGCAGCAAATCTTTTTACAAATGGAGAAACTGTACAAAGAATACTTAAATAATGGATTTAC
GGTTGTTAAGCTGTTATGGGAAAGTTATGCAGTGAGCATTGGAAAAGAAATTACAGCTCGTACAATGAGGCAAACGATTA
CCGGTGTAGCGAAAGGGATTACCGATGATGGCGTCTTACTATTAGAAGATCATCAAGGTTATATTCATCATATCCATTCT
GCTGATATAGAAATGAACTAA

Upstream 100 bases:

>100_bases
CAGGCCCTTGGGTAGCGGAAGTATTACAAAAAATTGAAGAAGAAATTTTGCAGAAGCGATTGGAAAATGAAAAAGAGGCG
ATAAGGGGGTGGATTGAAGA

Downstream 100 bases:

>100_bases
AAAGAAGGTTCTATGGTTTGGATTTGAAATAAAATGTAGTCATTTTTATTCTCAGTTTGCTATAATTATTTCGAATATGG
GCAGTATCGAAATGAACTGC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQSTMRKQLLQIFSEANGEFVSGQTISEKLGCSRTAVWKHMEDLRTEGYELEAIRRLGYRITSKPDKVTANEIQLGLQTD
FIGRTVYFEESVESTQHVAAKLAYEGAAEGTIVVAEEQTAGRGRLSRKWYSPKGTGVWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILINGKKTVGILTEMQADPDKINAVIMGIGINANQQTEHFVDEIKQIATSLAIESGKP
IVRAELMQQIFLQMEKLYKEYLNNGFTVVKLLWESYAVSIGKEITARTMRQTITGVAKGITDDGVLLLEDHQGYIHHIHS
ADIEMN

Sequences:

>Translated_326_residues
MQSTMRKQLLQIFSEANGEFVSGQTISEKLGCSRTAVWKHMEDLRTEGYELEAIRRLGYRITSKPDKVTANEIQLGLQTD
FIGRTVYFEESVESTQHVAAKLAYEGAAEGTIVVAEEQTAGRGRLSRKWYSPKGTGVWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILINGKKTVGILTEMQADPDKINAVIMGIGINANQQTEHFVDEIKQIATSLAIESGKP
IVRAELMQQIFLQMEKLYKEYLNNGFTVVKLLWESYAVSIGKEITARTMRQTITGVAKGITDDGVLLLEDHQGYIHHIHS
ADIEMN
>Mature_326_residues
MQSTMRKQLLQIFSEANGEFVSGQTISEKLGCSRTAVWKHMEDLRTEGYELEAIRRLGYRITSKPDKVTANEIQLGLQTD
FIGRTVYFEESVESTQHVAAKLAYEGAAEGTIVVAEEQTAGRGRLSRKWYSPKGTGVWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILINGKKTVGILTEMQADPDKINAVIMGIGINANQQTEHFVDEIKQIATSLAIESGKP
IVRAELMQQIFLQMEKLYKEYLNNGFTVVKLLWESYAVSIGKEITARTMRQTITGVAKGITDDGVLLLEDHQGYIHHIHS
ADIEMN

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=311, Percent_Identity=25.4019292604502, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1790408, Length=312, Percent_Identity=29.8076923076923, Blast_Score=117, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24644246, Length=234, Percent_Identity=29.0598290598291, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36225; Mature: 36225

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTMRKQLLQIFSEANGEFVSGQTISEKLGCSRTAVWKHMEDLRTEGYELEAIRRLGYR
CCHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCE
ITSKPDKVTANEIQLGLQTDFIGRTVYFEESVESTQHVAAKLAYEGAAEGTIVVAEEQTA
ECCCCCCEEHHHEEECCEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRLSRKWYSPKGTGVWMSIILRPSIPVHHAPQLTLLAAVSVAQAIEKCTGVNVGIKWP
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
NDILINGKKTVGILTEMQADPDKINAVIMGIGINANQQTEHFVDEIKQIATSLAIESGKP
CCEEECCCCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IVRAELMQQIFLQMEKLYKEYLNNGFTVVKLLWESYAVSIGKEITARTMRQTITGVAKGI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDDGVLLLEDHQGYIHHIHSADIEMN
CCCCEEEEECCCCEEEEEECCCCCCC
>Mature Secondary Structure
MQSTMRKQLLQIFSEANGEFVSGQTISEKLGCSRTAVWKHMEDLRTEGYELEAIRRLGYR
CCHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCE
ITSKPDKVTANEIQLGLQTDFIGRTVYFEESVESTQHVAAKLAYEGAAEGTIVVAEEQTA
ECCCCCCEEHHHEEECCEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRLSRKWYSPKGTGVWMSIILRPSIPVHHAPQLTLLAAVSVAQAIEKCTGVNVGIKWP
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
NDILINGKKTVGILTEMQADPDKINAVIMGIGINANQQTEHFVDEIKQIATSLAIESGKP
CCEEECCCCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IVRAELMQQIFLQMEKLYKEYLNNGFTVVKLLWESYAVSIGKEITARTMRQTITGVAKGI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDDGVLLLEDHQGYIHHIHSADIEMN
CCCCEEEEECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]