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The map label for this gene is cca

Identifier: 152975060

GI number: 152975060

Start: 1367337

End: 1368530

Strand: Direct

Name: cca

Synonym: Bcer98_1260

Alternate gene names: 152975060

Gene position: 1367337-1368530 (Clockwise)

Preceding gene: 152975059

Following gene: 152975061

Centisome position: 33.46

GC content: 36.93

Gene sequence:

>1194_bases
ATGAAACGATTTAAAAGAGCTGGAGCAATTATTGAGACGTTAAAGGAACATGGACATGAGGCCTACTTTGTAGGAGGAAG
TGTACGTGACTTTATTATTGATAGACCGATAGGAGATATTGATATTGCTACATCTGCTCTCCCAGAAGAAGTTATGAAAT
TATTTCCAAAGCACGTTCCTGTTGGTCTTGAACACGGAACGGTTATCGTTTTGCAAGATGGTGAGCCATATGAAGTAACT
ACTTTCCGTACAGAGAGTGATTATGAAGATTTTCGCAGGCCAAGTAGTGTACAATTTGTTCGCTCGTTGGAAGAAGATTT
AAAACGACGTGATTTTACAATGAATGCGATTGCGATGAATGAGGATGGAGAAATCATTGACTTATTTGGAGGTCAAGAAG
CCATTCAAAAACGAGAAATTGTGACAGTAGGAAATGCGGCAGAGCGTTTTCAAGAAGATGCGCTGCGCATGATGCGTGGT
ATTCGTTTTGTGAGTACATTAGGATTTTCTTTAGAAGAGAAAACAGAGTGTGCGATTAAGCGCTACGGTCATTTATTAGA
ACATATAGCGATTGAAAGAATTACTGTTGAATTTGAAAAGTTATTAACGGGTCCATACTGTGTAAAAGGACTTCAAAAAC
TAGTAGAGACGAAATTGTTTATGCATTTACCGTATTTACAAATGTCAGAAGAGAAAATTTTAAAGGCTGCGGAGTATAAC
TGGGAGTCTTTTGAAACAGAAATTGAAGCATGGGCGTTTTTCTTATCCTGTATAGGAGAGGAACATCCATCTGTATTTTT
ACGTCAATGGAAATTTTCAAATAAGAAAATAAAAGAAATTGTGGCTGTTTTGTTAGCTATTCGTACTCGAAAGACAAAAG
AATGGGATGCAGTTTTCTTATATCAAACTGGTGTACAAATTGCTCTCATGGCAGAGAGAGTCTATCAAGTGATGATAGAA
GAATACAATATGTCTACAGTAAGCGAAGTGCAAAGATTATTTGATTCATTACCAATTCAGAAGCGTCAAGAAATGAACGT
AAGTGGTAATGATTTATTAAGTTGGACAGATAAAACACCAGGCCCTTGGGTAGCGGAAGTATTACAAAAAATTGAAGAAG
AAATTTTGCAGAAGCGATTGGAAAATGAAAAAGAGGCGATAAGGGGGTGGATTGAAGAATGCAATCTACTATGA

Upstream 100 bases:

>100_bases
GCGGATCAAGCATTAGAAACGGTGCACGAGCAATTTCGTTCAGAAAATATCGTTTCACAATATGAAGCAATTTATTATGA
CATATTAAGGGATGACAACA

Downstream 100 bases:

>100_bases
GAAAACAATTGTTACAAATTTTTTCTGAGGCAAATGGAGAATTTGTATCTGGACAAACGATAAGTGAAAAGCTCGGTTGT
TCTAGAACGGCTGTGTGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKRFKRAGAIIETLKEHGHEAYFVGGSVRDFIIDRPIGDIDIATSALPEEVMKLFPKHVPVGLEHGTVIVLQDGEPYEVT
TFRTESDYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMNEDGEIIDLFGGQEAIQKREIVTVGNAAERFQEDALRMMRG
IRFVSTLGFSLEEKTECAIKRYGHLLEHIAIERITVEFEKLLTGPYCVKGLQKLVETKLFMHLPYLQMSEEKILKAAEYN
WESFETEIEAWAFFLSCIGEEHPSVFLRQWKFSNKKIKEIVAVLLAIRTRKTKEWDAVFLYQTGVQIALMAERVYQVMIE
EYNMSTVSEVQRLFDSLPIQKRQEMNVSGNDLLSWTDKTPGPWVAEVLQKIEEEILQKRLENEKEAIRGWIEECNLL

Sequences:

>Translated_397_residues
MKRFKRAGAIIETLKEHGHEAYFVGGSVRDFIIDRPIGDIDIATSALPEEVMKLFPKHVPVGLEHGTVIVLQDGEPYEVT
TFRTESDYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMNEDGEIIDLFGGQEAIQKREIVTVGNAAERFQEDALRMMRG
IRFVSTLGFSLEEKTECAIKRYGHLLEHIAIERITVEFEKLLTGPYCVKGLQKLVETKLFMHLPYLQMSEEKILKAAEYN
WESFETEIEAWAFFLSCIGEEHPSVFLRQWKFSNKKIKEIVAVLLAIRTRKTKEWDAVFLYQTGVQIALMAERVYQVMIE
EYNMSTVSEVQRLFDSLPIQKRQEMNVSGNDLLSWTDKTPGPWVAEVLQKIEEEILQKRLENEKEAIRGWIEECNLL
>Mature_397_residues
MKRFKRAGAIIETLKEHGHEAYFVGGSVRDFIIDRPIGDIDIATSALPEEVMKLFPKHVPVGLEHGTVIVLQDGEPYEVT
TFRTESDYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMNEDGEIIDLFGGQEAIQKREIVTVGNAAERFQEDALRMMRG
IRFVSTLGFSLEEKTECAIKRYGHLLEHIAIERITVEFEKLLTGPYCVKGLQKLVETKLFMHLPYLQMSEEKILKAAEYN
WESFETEIEAWAFFLSCIGEEHPSVFLRQWKFSNKKIKEIVAVLLAIRTRKTKEWDAVFLYQTGVQIALMAERVYQVMIE
EYNMSTVSEVQRLFDSLPIQKRQEMNVSGNDLLSWTDKTPGPWVAEVLQKIEEEILQKRLENEKEAIRGWIEECNLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=207, Percent_Identity=32.8502415458937, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87081691, Length=225, Percent_Identity=34.2222222222222, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789436, Length=190, Percent_Identity=34.2105263157895, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71995920, Length=269, Percent_Identity=28.996282527881, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281360112, Length=231, Percent_Identity=31.6017316017316, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21357337, Length=231, Percent_Identity=31.6017316017316, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BACCN (A7GN74)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374577.1
- ProteinModelPortal:   A7GN74
- SMR:   A7GN74
- STRING:   A7GN74
- EnsemblBacteria:   EBBACT00000038556
- GeneID:   5346002
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1260
- eggNOG:   COG0617
- GeneTree:   EBGT00050000000996
- HOGENOM:   HBG635063
- OMA:   YALLLYN
- ProtClustDB:   PRK13299
- BioCyc:   BCER315749:BCER98_1260-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46057; Mature: 46057

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 29-29 BINDING 110-110 BINDING 153-153 BINDING 156-156 BINDING 159-159 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFKRAGAIIETLKEHGHEAYFVGGSVRDFIIDRPIGDIDIATSALPEEVMKLFPKHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
VGLEHGTVIVLQDGEPYEVTTFRTESDYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMN
CCCCCCEEEEEECCCCEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEC
EDGEIIDLFGGQEAIQKREIVTVGNAAERFQEDALRMMRGIRFVSTLGFSLEEKTECAIK
CCCCEEEECCCHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RYGHLLEHIAIERITVEFEKLLTGPYCVKGLQKLVETKLFMHLPYLQMSEEKILKAAEYN
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WESFETEIEAWAFFLSCIGEEHPSVFLRQWKFSNKKIKEIVAVLLAIRTRKTKEWDAVFL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
YQTGVQIALMAERVYQVMIEEYNMSTVSEVQRLFDSLPIQKRQEMNVSGNDLLSWTDKTP
EECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEECCCCC
GPWVAEVLQKIEEEILQKRLENEKEAIRGWIEECNLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRFKRAGAIIETLKEHGHEAYFVGGSVRDFIIDRPIGDIDIATSALPEEVMKLFPKHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
VGLEHGTVIVLQDGEPYEVTTFRTESDYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMN
CCCCCCEEEEEECCCCEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEC
EDGEIIDLFGGQEAIQKREIVTVGNAAERFQEDALRMMRGIRFVSTLGFSLEEKTECAIK
CCCCEEEECCCHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RYGHLLEHIAIERITVEFEKLLTGPYCVKGLQKLVETKLFMHLPYLQMSEEKILKAAEYN
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WESFETEIEAWAFFLSCIGEEHPSVFLRQWKFSNKKIKEIVAVLLAIRTRKTKEWDAVFL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
YQTGVQIALMAERVYQVMIEEYNMSTVSEVQRLFDSLPIQKRQEMNVSGNDLLSWTDKTP
EECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEECCCCC
GPWVAEVLQKIEEEILQKRLENEKEAIRGWIEECNLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA