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The map label for this gene is hmp [H]

Identifier: 152974974

GI number: 152974974

Start: 1286995

End: 1288203

Strand: Direct

Name: hmp [H]

Synonym: Bcer98_1174

Alternate gene names: 152974974

Gene position: 1286995-1288203 (Clockwise)

Preceding gene: 152974973

Following gene: 152974976

Centisome position: 31.49

GC content: 37.06

Gene sequence:

>1209_bases
ATGTTAAGTCAAAAAACAATTGAAATTGTAAAATCAACAGTACCATTATTACAAGAAAAAGGTGTTGATATCACGACACG
CTTTTATCAAATTATGTTTGCGGAACACCCGGAGTTATTAAATATTTTTAATCATACAAACCAGAAAATAGGAAGACAAC
AACAAGCGTTAGCAAGTGCAGTGTGTGCGGCGGCTATGTATATTGATCACTTGGAAGCCATTATTCCTGTTGTCAAACAG
ATTGGTCATAAACATAGAAGCTTAGGAATTAAAGCTGAACATTATCCAATTGTAGGAAATTGTTTATTACAGGCAATTAA
AGAGGTAGCAGGCGCACCGCAAGAAGTGTTGGATGCTTGGGGCGAAGCGTATGGTGTAATTGCTGATGCATTTATTCGTA
TCGAGGCGGAAATGTATGAAGAAGCAGCAAAAAAAGAAGGCGGCTGGAAAGAGTTACGTAACTTTATTGTTGTGAAAAAA
GAGAAAGAGAGCGACGTTATTACATCGTTTTACTTAAAACCAGAAGACGGTGGAGAACTATCAATGTTTTTACCTGGTCA
GTATGTAACGGTTCAAATTCGTATAGAAGGAGAAACGTATATACATAATCGCCAGTATAGTTTATCGGATGCTCCTGGAA
AAGAGTATTATCGTATTAGTGTAAAACGTGAAACAAGTGCAGAAGCACCGGATGGGAAAGTTTCGAATTATTTACACGAC
CATGTTCACGAAGGAGATATTTTGCCACTCAGTGCACCAGCTGGAGACTTTGTATTAAATATAGATTCAAACTTACCGGT
TGTATTAATAAGCGGTGGTGTAGGAATTACGCCTATGATGAGTATGTTTAATACGTTAACTGAGAGAGAAATGGACCGCG
ATGTATACTTTATTCATGCAGCGTTAAATAGTAAAGTCCATGCAATGAAAGAGCATGTCCAAAAGGTAGCAGAAAGTAAA
GAAAGAGTGAAAGTATATACTTGTTATTCAGCACCAACAAAGCAAGATGTACAAGCGGAAAATTTTGAGAAAGAAGGACT
TATTGATTTAGCTTGGTTACAATCTATTATTCCTTCAAATGAAGCGGAATTTTATTTCTGTGGTCCAGTACCATTTATGA
AACATATCAATGCAGTGTTACTAGAGTGGGGAGTTCCAGCTGATCGTATTCACTATGAGTTCTTTGGACCAGCAGTTAGC
TTACAATAA

Upstream 100 bases:

>100_bases
TTTCACAAAAACACCGTGTTTATAGTGATTAAAATCACAACGTTCCATCAAAAACTCTTTTACACTAACAGTAGTCAAGG
AAACAGAGGAGTGAGATACA

Downstream 100 bases:

>100_bases
AGAGAATTTACCAAATCAAAAACGAGGTTATTTCACCTCGTTTTTGATTTGATCTCGTAATAAACGATTTACGGTTTCAC
GTCGCACACCAATGAGTTGA

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLSQKTIEIVKSTVPLLQEKGVDITTRFYQIMFAEHPELLNIFNHTNQKIGRQQQALASAVCAAAMYIDHLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGNCLLQAIKEVAGAPQEVLDAWGEAYGVIADAFIRIEAEMYEEAAKKEGGWKELRNFIVVKK
EKESDVITSFYLKPEDGGELSMFLPGQYVTVQIRIEGETYIHNRQYSLSDAPGKEYYRISVKRETSAEAPDGKVSNYLHD
HVHEGDILPLSAPAGDFVLNIDSNLPVVLISGGVGITPMMSMFNTLTEREMDRDVYFIHAALNSKVHAMKEHVQKVAESK
ERVKVYTCYSAPTKQDVQAENFEKEGLIDLAWLQSIIPSNEAEFYFCGPVPFMKHINAVLLEWGVPADRIHYEFFGPAVS
LQ

Sequences:

>Translated_402_residues
MLSQKTIEIVKSTVPLLQEKGVDITTRFYQIMFAEHPELLNIFNHTNQKIGRQQQALASAVCAAAMYIDHLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGNCLLQAIKEVAGAPQEVLDAWGEAYGVIADAFIRIEAEMYEEAAKKEGGWKELRNFIVVKK
EKESDVITSFYLKPEDGGELSMFLPGQYVTVQIRIEGETYIHNRQYSLSDAPGKEYYRISVKRETSAEAPDGKVSNYLHD
HVHEGDILPLSAPAGDFVLNIDSNLPVVLISGGVGITPMMSMFNTLTEREMDRDVYFIHAALNSKVHAMKEHVQKVAESK
ERVKVYTCYSAPTKQDVQAENFEKEGLIDLAWLQSIIPSNEAEFYFCGPVPFMKHINAVLLEWGVPADRIHYEFFGPAVS
LQ
>Mature_402_residues
MLSQKTIEIVKSTVPLLQEKGVDITTRFYQIMFAEHPELLNIFNHTNQKIGRQQQALASAVCAAAMYIDHLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGNCLLQAIKEVAGAPQEVLDAWGEAYGVIADAFIRIEAEMYEEAAKKEGGWKELRNFIVVKK
EKESDVITSFYLKPEDGGELSMFLPGQYVTVQIRIEGETYIHNRQYSLSDAPGKEYYRISVKRETSAEAPDGKVSNYLHD
HVHEGDILPLSAPAGDFVLNIDSNLPVVLISGGVGITPMMSMFNTLTEREMDRDVYFIHAALNSKVHAMKEHVQKVAESK
ERVKVYTCYSAPTKQDVQAENFEKEGLIDLAWLQSIIPSNEAEFYFCGPVPFMKHINAVLLEWGVPADRIHYEFFGPAVS
LQ

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=397, Percent_Identity=45.3400503778337, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI1787658, Length=255, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788104, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=414, Percent_Identity=42.7536231884058, Blast_Score=275, Evalue=8e-75,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 45183; Mature: 45183

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQKTIEIVKSTVPLLQEKGVDITTRFYQIMFAEHPELLNIFNHTNQKIGRQQQALASA
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VCAAAMYIDHLEAIIPVVKQIGHKHRSLGIKAEHYPIVGNCLLQAIKEVAGAPQEVLDAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFIRIEAEMYEEAAKKEGGWKELRNFIVVKKEKESDVITSFYLKPEDGGEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCEEEEEEECCCCCCEE
SMFLPGQYVTVQIRIEGETYIHNRQYSLSDAPGKEYYRISVKRETSAEAPDGKVSNYLHD
EEEECCEEEEEEEEECCCEEEECCEEECCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
HVHEGDILPLSAPAGDFVLNIDSNLPVVLISGGVGITPMMSMFNTLTEREMDRDVYFIHA
HCCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEE
ALNSKVHAMKEHVQKVAESKERVKVYTCYSAPTKQDVQAENFEKEGLIDLAWLQSIIPSN
HHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLLEWGVPADRIHYEFFGPAVSLQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHEEEEEECCEECCC
>Mature Secondary Structure
MLSQKTIEIVKSTVPLLQEKGVDITTRFYQIMFAEHPELLNIFNHTNQKIGRQQQALASA
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VCAAAMYIDHLEAIIPVVKQIGHKHRSLGIKAEHYPIVGNCLLQAIKEVAGAPQEVLDAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFIRIEAEMYEEAAKKEGGWKELRNFIVVKKEKESDVITSFYLKPEDGGEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCEEEEEEECCCCCCEE
SMFLPGQYVTVQIRIEGETYIHNRQYSLSDAPGKEYYRISVKRETSAEAPDGKVSNYLHD
EEEECCEEEEEEEEECCCEEEECCEEECCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
HVHEGDILPLSAPAGDFVLNIDSNLPVVLISGGVGITPMMSMFNTLTEREMDRDVYFIHA
HCCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEE
ALNSKVHAMKEHVQKVAESKERVKVYTCYSAPTKQDVQAENFEKEGLIDLAWLQSIIPSN
HHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLLEWGVPADRIHYEFFGPAVSLQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHEEEEEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]