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The map label for this gene is nirA [H]

Identifier: 152974949

GI number: 152974949

Start: 1256855

End: 1258477

Strand: Direct

Name: nirA [H]

Synonym: Bcer98_1146

Alternate gene names: 152974949

Gene position: 1256855-1258477 (Clockwise)

Preceding gene: 152974948

Following gene: 152974950

Centisome position: 30.75

GC content: 37.03

Gene sequence:

>1623_bases
ATGAGTTATGAAAAAGTATGGGCAAGCAATGAGAAATTAAATAGGACAGAGAAAAAGAAACTAGAGAAAGATGGTTTGGA
GATTTTTAAAGAGATTCCGTATTATGCTGAAAACGGCTTCGCATCTATTCCGAAAGAAGAATGGGATTCCTTTAAATGGG
CGGGGTTATACTTGCAACGTCCAAAAGAGGCAGGGTACTTTATGATGCGTGTTAATATCCCTTCTGGTATTTTAACGAAT
GAACAAGTGGAAGTATTAGCTTCTATTGCAGAAGACTATGGCCGTGATGTTCTCGATATTACGACAAGACAGGCAATTCA
ATTTCATTGGTTACAAATTGAACAAATACCGGATATTTTTAAGCGTTTAGAAAGCGTAGGATTATCAACAGCAGGAGCGT
GCGGAGATATTACAAGAAATATTATCGGAAATCCGCTTGCTGGTATTGATCGTGATGAGCTCTTTGATACAGCGCCAATT
GTAAAAGACATATACGAGTTCTTTCAACATAATGAGGATTTTTCGAATTTACCACGGAAATTTAAAATATCTATTAGTGC
TAATGTATATAATGCAGCAAATGCTGAAATTAATTGTGCAGCTTTTGTACCAGCGCGGAAAGAAATAGATGGAAATATAG
TCGAAGGGTTTCATGTGCGAGTTGGTGGTGGATTATCAGCGCGTCCTTATTTAGCACAAGAACTCGATCTCTTTATTCGC
CCTCAGCAGGTGAAGGACGTAGCGATTGCTATTGCGACTATTTTTCGGGATTTTGGATATCGTGAAAAACGCCATTTGGC
TCGTTTGAAATTTTTAGTAGCAGATTGGGGGCCGGAAAAATTTAAAGAAACATTACTAGAATATACAGGCCCTTTATATG
AAAAGGGAGAGAGTGCTCTAAAAGGATGGAATGCCGGTTATTTTTATGGTGTGCATAAACAAAAGCAGGAAAATCTATCA
TATGTAGGCTTTAATGTGCCTGTTGGGCGGTTACATTCGGAGGAGTTCTTTGAAATTGCAAGAATTGCTAAGCAGTATGG
AAATGGAGAAATTCGTACATGTAATTCACAAAACTTCATTATTCCAAATGTACCAGAAGAAAATGTAGAAAGTCTATTAA
AAGAACCATTGTTTGAATCCATATCAGCAAGTCCAAAATCTTTCATCGGTTATGCTGTATCATGTACGGGAATTGAATAT
TGTAATCTAGCATTAGTGGAAACGAAAGAAAGGTTACGGCAAATTGCAGATTATTTAGATACGCAACTTGCATTAGATGT
TCCTGTTCGAATCCATATGGTAGGCTGTCCGAATTCATGCGGGCAACGGCAAATTGCAGATATTGGATTACAAGGTGTAA
AGCTTAAAACGAAAGAAAAAGGGATTGTTGAAGCCTTTGAAATATATGTAGGTGGAACGTTGCTTGAAGGTGGTGCCTAT
AATCAAAAATTAAAAGGGAAAATTGATGGAGAAGATCTTTCAAATGTACTGGTGGCATTTTTAACTTATTTTAAAGAGCA
TAAATTGCCAGCTGAAACATTCTATGAGTTTGTAGGTCGTGTAGGGATTGAAAATTTACAAGAGGCATTCAATACCATTA
TAGAAGAAGGAATTGCATCATAG

Upstream 100 bases:

>100_bases
CGGAATTAACTGTGGAAACAAACCGTTATTCCATTGAAGAGTGTGCGGAACAAATCATTCATTATTTACAAGAACGTAGC
TTCATATAGGGGGATAGATT

Downstream 100 bases:

>100_bases
GGGGGCTAACGTATGGATTTATATCGTTTTGAAGCTGTTTTAGTAAATGGTGTTGTGCCTGTAGTTGTCGTTGCGCAAAG
TGAAGAACAAGCATTTCGAC

Product: ferredoxin--nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSYEKVWASNEKLNRTEKKKLEKDGLEIFKEIPYYAENGFASIPKEEWDSFKWAGLYLQRPKEAGYFMMRVNIPSGILTN
EQVEVLASIAEDYGRDVLDITTRQAIQFHWLQIEQIPDIFKRLESVGLSTAGACGDITRNIIGNPLAGIDRDELFDTAPI
VKDIYEFFQHNEDFSNLPRKFKISISANVYNAANAEINCAAFVPARKEIDGNIVEGFHVRVGGGLSARPYLAQELDLFIR
PQQVKDVAIAIATIFRDFGYREKRHLARLKFLVADWGPEKFKETLLEYTGPLYEKGESALKGWNAGYFYGVHKQKQENLS
YVGFNVPVGRLHSEEFFEIARIAKQYGNGEIRTCNSQNFIIPNVPEENVESLLKEPLFESISASPKSFIGYAVSCTGIEY
CNLALVETKERLRQIADYLDTQLALDVPVRIHMVGCPNSCGQRQIADIGLQGVKLKTKEKGIVEAFEIYVGGTLLEGGAY
NQKLKGKIDGEDLSNVLVAFLTYFKEHKLPAETFYEFVGRVGIENLQEAFNTIIEEGIAS

Sequences:

>Translated_540_residues
MSYEKVWASNEKLNRTEKKKLEKDGLEIFKEIPYYAENGFASIPKEEWDSFKWAGLYLQRPKEAGYFMMRVNIPSGILTN
EQVEVLASIAEDYGRDVLDITTRQAIQFHWLQIEQIPDIFKRLESVGLSTAGACGDITRNIIGNPLAGIDRDELFDTAPI
VKDIYEFFQHNEDFSNLPRKFKISISANVYNAANAEINCAAFVPARKEIDGNIVEGFHVRVGGGLSARPYLAQELDLFIR
PQQVKDVAIAIATIFRDFGYREKRHLARLKFLVADWGPEKFKETLLEYTGPLYEKGESALKGWNAGYFYGVHKQKQENLS
YVGFNVPVGRLHSEEFFEIARIAKQYGNGEIRTCNSQNFIIPNVPEENVESLLKEPLFESISASPKSFIGYAVSCTGIEY
CNLALVETKERLRQIADYLDTQLALDVPVRIHMVGCPNSCGQRQIADIGLQGVKLKTKEKGIVEAFEIYVGGTLLEGGAY
NQKLKGKIDGEDLSNVLVAFLTYFKEHKLPAETFYEFVGRVGIENLQEAFNTIIEEGIAS
>Mature_539_residues
SYEKVWASNEKLNRTEKKKLEKDGLEIFKEIPYYAENGFASIPKEEWDSFKWAGLYLQRPKEAGYFMMRVNIPSGILTNE
QVEVLASIAEDYGRDVLDITTRQAIQFHWLQIEQIPDIFKRLESVGLSTAGACGDITRNIIGNPLAGIDRDELFDTAPIV
KDIYEFFQHNEDFSNLPRKFKISISANVYNAANAEINCAAFVPARKEIDGNIVEGFHVRVGGGLSARPYLAQELDLFIRP
QQVKDVAIAIATIFRDFGYREKRHLARLKFLVADWGPEKFKETLLEYTGPLYEKGESALKGWNAGYFYGVHKQKQENLSY
VGFNVPVGRLHSEEFFEIARIAKQYGNGEIRTCNSQNFIIPNVPEENVESLLKEPLFESISASPKSFIGYAVSCTGIEYC
NLALVETKERLRQIADYLDTQLALDVPVRIHMVGCPNSCGQRQIADIGLQGVKLKTKEKGIVEAFEIYVGGTLLEGGAYN
QKLKGKIDGEDLSNVLVAFLTYFKEHKLPAETFYEFVGRVGIENLQEAFNTIIEEGIAS

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=512, Percent_Identity=25.1953125, Blast_Score=118, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6322597, Length=530, Percent_Identity=22.8301886792453, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 60963; Mature: 60832

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEKVWASNEKLNRTEKKKLEKDGLEIFKEIPYYAENGFASIPKEEWDSFKWAGLYLQR
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHCCEEEEEEEEEC
PKEAGYFMMRVNIPSGILTNEQVEVLASIAEDYGRDVLDITTRQAIQFHWLQIEQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRLESVGLSTAGACGDITRNIIGNPLAGIDRDELFDTAPIVKDIYEFFQHNEDFSNLPRK
HHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCE
FKISISANVYNAANAEINCAAFVPARKEIDGNIVEGFHVRVGGGLSARPYLAQELDLFIR
EEEEEEEEEEECCCCCEEEEEECCCHHHCCCCEECCEEEEECCCCCCCCHHHHHHHHHCC
PQQVKDVAIAIATIFRDFGYREKRHLARLKFLVADWGPEKFKETLLEYTGPLYEKGESAL
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCHHHH
KGWNAGYFYGVHKQKQENLSYVGFNVPVGRLHSEEFFEIARIAKQYGNGEIRTCNSQNFI
CCCCCCEEEECCHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCE
IPNVPEENVESLLKEPLFESISASPKSFIGYAVSCTGIEYCNLALVETKERLRQIADYLD
ECCCCHHHHHHHHHHHHHHHHCCCCHHHHCEEEEECCHHHHCCHHHHHHHHHHHHHHHHC
TQLALDVPVRIHMVGCPNSCGQRQIADIGLQGVKLKTKEKGIVEAFEIYVGGTLLEGGAY
CCEEEECCEEEEEEECCCCCCCHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEECCCCC
NQKLKGKIDGEDLSNVLVAFLTYFKEHKLPAETFYEFVGRVGIENLQEAFNTIIEEGIAS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYEKVWASNEKLNRTEKKKLEKDGLEIFKEIPYYAENGFASIPKEEWDSFKWAGLYLQR
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHCCEEEEEEEEEC
PKEAGYFMMRVNIPSGILTNEQVEVLASIAEDYGRDVLDITTRQAIQFHWLQIEQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRLESVGLSTAGACGDITRNIIGNPLAGIDRDELFDTAPIVKDIYEFFQHNEDFSNLPRK
HHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCE
FKISISANVYNAANAEINCAAFVPARKEIDGNIVEGFHVRVGGGLSARPYLAQELDLFIR
EEEEEEEEEEECCCCCEEEEEECCCHHHCCCCEECCEEEEECCCCCCCCHHHHHHHHHCC
PQQVKDVAIAIATIFRDFGYREKRHLARLKFLVADWGPEKFKETLLEYTGPLYEKGESAL
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCHHHH
KGWNAGYFYGVHKQKQENLSYVGFNVPVGRLHSEEFFEIARIAKQYGNGEIRTCNSQNFI
CCCCCCEEEECCHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCE
IPNVPEENVESLLKEPLFESISASPKSFIGYAVSCTGIEYCNLALVETKERLRQIADYLD
ECCCCHHHHHHHHHHHHHHHHCCCCHHHHCEEEEECCHHHHCCHHHHHHHHHHHHHHHHC
TQLALDVPVRIHMVGCPNSCGQRQIADIGLQGVKLKTKEKGIVEAFEIYVGGTLLEGGAY
CCEEEECCEEEEEEECCCCCCCHHHHHCCCCCCEEECCCCCHHHHHHHHCCCEEECCCCC
NQKLKGKIDGEDLSNVLVAFLTYFKEHKLPAETFYEFVGRVGIENLQEAFNTIIEEGIAS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]