Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 152974936

GI number: 152974936

Start: 1245401

End: 1246159

Strand: Direct

Name: hisF

Synonym: Bcer98_1133

Alternate gene names: 152974936

Gene position: 1245401-1246159 (Clockwise)

Preceding gene: 152974935

Following gene: 152974937

Centisome position: 30.47

GC content: 38.74

Gene sequence:

>759_bases
ATGTTAGCAAAGCGAATTATTCCCTGCTTAGATGTGAAAGCGGGCCGCGTTGTGAAGGGAGTCAATTTTATCGGATTACA
AGATATAGGAGACCCTGTTGAAATAGCTGCTTTGTATAATCAAGCGGGTGCAGATGAAATTGTTTTTTTAGATATTACAG
CCACATATGAAGAGAGAAAAACAATTGTGGATGTTGTTGAAAAAACAGCTTCGAAAGTGTTTATTCCATTAACAGTTGGA
GGCGGTATTTCTACAGTTAAAGATATATATACATTGTTAAGAGCAGGTGCTGATAAAGTTTCACTGAATTCAGCCGCTGT
GAAAAATCCATTATTCATTCAAGAAGGAGCAGAGCACTTTGGTTCCCAATGTATTGTCGTCGCAATTGATGCAAGAAAAG
TAGAAGAAGACAAATGGCACGTATATGTAAACGGTGGAAGAACGGATACAGGAATTGATGCGGTTCATTGGGCGAAACAA
GTGGCTGAACTTGGGGCAGGGGAAATTTTATTAACAAGCATGGATGCCGATGGAACGAAAGATGGGTATAATCTTCGTTT
AACAGACATCATTTCTGCAAATGTCTCCATACCTGTTATTGCTTCAGGTGGATGTGGAAGTGCTAATCATATTGTTGAAG
TATTTGCGCATACATCTGTAAATGCAGCGCTAGCAGCATCCATTTTTCATTATGGAGAATGCACAGTACAGGAAGTAAAA
CAAAAATTACAATATGCAGGTATTGAGGTGAGAATATGA

Upstream 100 bases:

>100_bases
TAGAACAATTACAGGCAATGAATATGTACGGTGTGATTATTGGAAAAGCGCTGTACGAAAAAACAATGGATCTACAAGAA
GTATTGCGGGTGACAAAGTC

Downstream 100 bases:

>100_bases
GACCTAATTTTTCTAAAGGATTATTACCTGCTATTGTTATAAATGAGGAGACAAAAGAAGTTTTAATGCTAGCTTATATG
AATGAAGAGGCATATGAAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVKAGRVVKGVNFIGLQDIGDPVEIAALYNQAGADEIVFLDITATYEERKTIVDVVEKTASKVFIPLTVG
GGISTVKDIYTLLRAGADKVSLNSAAVKNPLFIQEGAEHFGSQCIVVAIDARKVEEDKWHVYVNGGRTDTGIDAVHWAKQ
VAELGAGEILLTSMDADGTKDGYNLRLTDIISANVSIPVIASGGCGSANHIVEVFAHTSVNAALAASIFHYGECTVQEVK
QKLQYAGIEVRI

Sequences:

>Translated_252_residues
MLAKRIIPCLDVKAGRVVKGVNFIGLQDIGDPVEIAALYNQAGADEIVFLDITATYEERKTIVDVVEKTASKVFIPLTVG
GGISTVKDIYTLLRAGADKVSLNSAAVKNPLFIQEGAEHFGSQCIVVAIDARKVEEDKWHVYVNGGRTDTGIDAVHWAKQ
VAELGAGEILLTSMDADGTKDGYNLRLTDIISANVSIPVIASGGCGSANHIVEVFAHTSVNAALAASIFHYGECTVQEVK
QKLQYAGIEVRI
>Mature_252_residues
MLAKRIIPCLDVKAGRVVKGVNFIGLQDIGDPVEIAALYNQAGADEIVFLDITATYEERKTIVDVVEKTASKVFIPLTVG
GGISTVKDIYTLLRAGADKVSLNSAAVKNPLFIQEGAEHFGSQCIVVAIDARKVEEDKWHVYVNGGRTDTGIDAVHWAKQ
VAELGAGEILLTSMDADGTKDGYNLRLTDIISANVSIPVIASGGCGSANHIVEVFAHTSVNAALAASIFHYGECTVQEVK
QKLQYAGIEVRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.9688715953307, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082028, Length=237, Percent_Identity=28.2700421940928, Blast_Score=88, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=32.5949367088608, Blast_Score=152, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BACCN (A7GMV0)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374453.1
- ProteinModelPortal:   A7GMV0
- SMR:   A7GMV0
- STRING:   A7GMV0
- EnsemblBacteria:   EBBACT00000038221
- GeneID:   5344521
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1133
- eggNOG:   COG0107
- GeneTree:   EBGT00050000001074
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   BCER315749:BCER98_1133-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26897; Mature: 26897

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKAGRVVKGVNFIGLQDIGDPVEIAALYNQAGADEIVFLDITATYEERK
CCHHHCCCCCCCCCCCEEECCEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECHHHHH
TIVDVVEKTASKVFIPLTVGGGISTVKDIYTLLRAGADKVSLNSAAVKNPLFIQEGAEHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCCEECCHHHHCCCEEEEHHHHHH
GSQCIVVAIDARKVEEDKWHVYVNGGRTDTGIDAVHWAKQVAELGAGEILLTSMDADGTK
CCEEEEEEEECEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
DGYNLRLTDIISANVSIPVIASGGCGSANHIVEVFAHTSVNAALAASIFHYGECTVQEVK
CCCEEEEEEEEECCCEEEEEECCCCCCHHHHEEEEHHCCCHHHHHHHHHHCCCCHHHHHH
QKLQYAGIEVRI
HHHHHCCCEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKAGRVVKGVNFIGLQDIGDPVEIAALYNQAGADEIVFLDITATYEERK
CCHHHCCCCCCCCCCCEEECCEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECHHHHH
TIVDVVEKTASKVFIPLTVGGGISTVKDIYTLLRAGADKVSLNSAAVKNPLFIQEGAEHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCCEECCHHHHCCCEEEEHHHHHH
GSQCIVVAIDARKVEEDKWHVYVNGGRTDTGIDAVHWAKQVAELGAGEILLTSMDADGTK
CCEEEEEEEECEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
DGYNLRLTDIISANVSIPVIASGGCGSANHIVEVFAHTSVNAALAASIFHYGECTVQEVK
CCCEEEEEEEEECCCEEEEEECCCCCCHHHHEEEEHHCCCHHHHHHHHHHCCCCHHHHHH
QKLQYAGIEVRI
HHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA