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The map label for this gene is leuD

Identifier: 152974930

GI number: 152974930

Start: 1239439

End: 1240020

Strand: Direct

Name: leuD

Synonym: Bcer98_1126

Alternate gene names: 152974930

Gene position: 1239439-1240020 (Clockwise)

Preceding gene: 152974929

Following gene: 152974931

Centisome position: 30.33

GC content: 36.08

Gene sequence:

>582_bases
ATGATGGAGCCATTTCGTACTCATAAAGGTACTGTCGCTGTACTTATGAACGACAATATTGATACCGATCAAATTATTCC
GAAACAATATTTAAAGAGGATCGAGCGAACTGGGTTTGGAAAATTTTTATTTGATGAATGGCGGTATGAAGAGGATAGAA
AAGAAAATCCACATTTTCCGCTCAATGACCCTGAACGAAAAGGAGCAAGTATTCTCATTACAGGAGAAAATTTTGGTTGT
GGATCATCGAGAGAACATGCACCATGGGCCCTTGCTGATTATGGATTTCGCGTTATTATTGCTGGAGGATTTGCAGATAT
TTTCTACATGAATTGTATGAAAAATGGAATGTTACCGATTGTAATGGACAAAGAGACGCGAGAAAAATTGGCAGCGATTG
AGGCGAGGACTATAGTAGAAGTTGATTTGGAAAATGAGGTAATCATGACAACGCAGCATCAATTTCATTTTTCTATTGAA
AAAATGTGGAGAGAAAAATTGTTAAAAGGTTTAGATGAGATTGAGATTACGATGCAATATGAAGAGTATATTCAAGAATA
TGAGGAAAAGGTAGTACATTAA

Upstream 100 bases:

>100_bases
GGGAGACAAGGAAAAGGTGCAAGAACACACCTTGTAAGTCCTGCAATGGCTGCGGCGGCAGCATTATATGGTCATTTCGT
CGATGTGAGAAAGGAGAGTT

Downstream 100 bases:

>100_bases
ACAATGTCAAAAAATATAAAGTTATAATTGTATTTACTTTCTGAATAGTTTGTTATATAATGTGTGAAAATAAGCGGAGG
AAAGGAGTAAGGATTATGTT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MMEPFRTHKGTVAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYEEDRKENPHFPLNDPERKGASILITGENFGC
GSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKETREKLAAIEARTIVEVDLENEVIMTTQHQFHFSIE
KMWREKLLKGLDEIEITMQYEEYIQEYEEKVVH

Sequences:

>Translated_193_residues
MMEPFRTHKGTVAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYEEDRKENPHFPLNDPERKGASILITGENFGC
GSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKETREKLAAIEARTIVEVDLENEVIMTTQHQFHFSIE
KMWREKLLKGLDEIEITMQYEEYIQEYEEKVVH
>Mature_193_residues
MMEPFRTHKGTVAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYEEDRKENPHFPLNDPERKGASILITGENFGC
GSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPIVMDKETREKLAAIEARTIVEVDLENEVIMTTQHQFHFSIE
KMWREKLLKGLDEIEITMQYEEYIQEYEEKVVH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=47.6923076923077, Blast_Score=190, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=47.9591836734694, Blast_Score=171, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_BACCN (A7GMU4)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374447.1
- ProteinModelPortal:   A7GMU4
- SMR:   A7GMU4
- STRING:   A7GMU4
- EnsemblBacteria:   EBBACT00000039229
- GeneID:   5344482
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1126
- eggNOG:   COG0066
- GeneTree:   EBGT00050000002923
- HOGENOM:   HBG304838
- OMA:   TVDLTTC
- ProtClustDB:   PRK01641
- BioCyc:   BCER315749:BCER98_1126-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22718; Mature: 22718

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEPFRTHKGTVAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYEEDRKENPHFP
CCCCCCCCCCEEEEEEECCCCCHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCC
LNDPERKGASILITGENFGCGSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPI
CCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCEEE
VMDKETREKLAAIEARTIVEVDLENEVIMTTQHQFHFSIEKMWREKLLKGLDEIEITMQY
EECCHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEHH
EEYIQEYEEKVVH
HHHHHHHHHHHCC
>Mature Secondary Structure
MMEPFRTHKGTVAVLMNDNIDTDQIIPKQYLKRIERTGFGKFLFDEWRYEEDRKENPHFP
CCCCCCCCCCEEEEEEECCCCCHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCC
LNDPERKGASILITGENFGCGSSREHAPWALADYGFRVIIAGGFADIFYMNCMKNGMLPI
CCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHCCCEEE
VMDKETREKLAAIEARTIVEVDLENEVIMTTQHQFHFSIEKMWREKLLKGLDEIEITMQY
EECCHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEHH
EEYIQEYEEKVVH
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA