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The map label for this gene is leuC

Identifier: 152974929

GI number: 152974929

Start: 1238070

End: 1239464

Strand: Direct

Name: leuC

Synonym: Bcer98_1125

Alternate gene names: 152974929

Gene position: 1238070-1239464 (Clockwise)

Preceding gene: 152974928

Following gene: 152974930

Centisome position: 30.29

GC content: 41.58

Gene sequence:

>1395_bases
ATGGGAAAGAGACTACTAGATAAACTATGGGAAAAGCATATTGTAGCAACAAATGAAAATGGACTGGATCTATTATATAT
TGATCTTCATCTTGTGCATGAAGTTACTTCACCGCAAGCCTTTGAAGGCTTGCGGCTTGCAAATCGTACAGTGCGGAGAC
CAGATTTAACATTTGCGACGATGGATCATAATATTCCGACAAAAGACGTTTGGAATATTACAGATCATATTGCTAAGCAA
CAACTAGATACATTGCGTGCAAATTGCAAACAATTTCATATATCGTTAGCCGATATTGGAGATGAAGCACAAGGAATTGT
TCATGTTATTGGGCCAGAACTTGGTCTTACGGTGCCAGGAAAGACAATTGTATGCGGTGATAGCCATACAGCAACGCATG
GAGCATTTAGTGCACTAGCTTTTGGAATCGGTACAAGTGAAGTTGAACATGTATTAGCGACGCAAACGCTATGGCAACGG
AAACCGAAGGCGATGGGAATTGAACTAAAAGGTACATTGCCGAAAGGGGTATATGCAAAAGATATCATTTTACATATTCT
TGCCACATATGGAGTAGCAGTTGGTACTGGATATGTGATGGAGTTTTATGGAGAAACAATTAGGAGTATGGAAATGGAAG
AACGGATGACACTTTGTAATATGGCGATTGAAGGTGGAGCAAAAGCAGGGATAATTGCGCCAGATGAAAAAACATTTGCT
TACGTAAAGGGTCGGAAATACGCACCGAAGGACTTTGAGCGTGCCGTAGAAAAGTGGAGTGAACTGTATACAGATTCAGA
TGCGGTGTATGATATGCACATTTCAATTGATGTATCAAAGTTAGCGCCTTATGTAACGTGGGGAACAAATCCGAGTATGG
GGATTCGTATCGATGAACAGTTACCAGCAATAAGCGATCAAAATGAAGAAAGAGCGTTTGCTTATATGGGACTGAAACCG
AAGCAAAGTGCATATGACATCCCGATTAAACATGTCTTTATTGGTTCTTGTACAAATTCAAGGCTGTCGGATTTAGAGAT
TGCAGCTGCGATTGTAAAAGGGAAGAAAGTAAAAGACGGCGTAAGAGCACTTGTTGTACCAGGATCACAAAAAGTAAGAC
AATTAGCGATGGAAAAGGGACTACATCATATATTTGAGGAAGCTGGATTTGAATGGCGGGAGCCAGGCTGTTCCATGTGT
CTTGGGATGAACCCAGATCAAGTACCTGCAGGAGAACATTGTGCTTCTACTTCAAACCGCAACTTTGAAGGGAGACAAGG
AAAAGGTGCAAGAACACACCTTGTAAGTCCTGCAATGGCTGCGGCGGCAGCATTATATGGTCATTTCGTCGATGTGAGAA
AGGAGAGTTATGATGGAGCCATTTCGTACTCATAA

Upstream 100 bases:

>100_bases
AATATACAGCTGATCTTGGCGGAGAAGAAACGACAACTTCATTTACACAAGCTGTCCTTCAAGAAATGGAAGAACAAGCG
CTAGTTGGGAGAGGGCGATA

Downstream 100 bases:

>100_bases
AGGTACTGTCGCTGTACTTATGAACGACAATATTGATACCGATCAAATTATTCCGAAACAATATTTAAAGAGGATCGAGC
GAACTGGGTTTGGAAAATTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGKRLLDKLWEKHIVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLANRTVRRPDLTFATMDHNIPTKDVWNITDHIAKQ
QLDTLRANCKQFHISLADIGDEAQGIVHVIGPELGLTVPGKTIVCGDSHTATHGAFSALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGTLPKGVYAKDIILHILATYGVAVGTGYVMEFYGETIRSMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPKDFERAVEKWSELYTDSDAVYDMHISIDVSKLAPYVTWGTNPSMGIRIDEQLPAISDQNEERAFAYMGLKP
KQSAYDIPIKHVFIGSCTNSRLSDLEIAAAIVKGKKVKDGVRALVVPGSQKVRQLAMEKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPAGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDVRKESYDGAISYS

Sequences:

>Translated_464_residues
MGKRLLDKLWEKHIVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLANRTVRRPDLTFATMDHNIPTKDVWNITDHIAKQ
QLDTLRANCKQFHISLADIGDEAQGIVHVIGPELGLTVPGKTIVCGDSHTATHGAFSALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGTLPKGVYAKDIILHILATYGVAVGTGYVMEFYGETIRSMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPKDFERAVEKWSELYTDSDAVYDMHISIDVSKLAPYVTWGTNPSMGIRIDEQLPAISDQNEERAFAYMGLKP
KQSAYDIPIKHVFIGSCTNSRLSDLEIAAAIVKGKKVKDGVRALVVPGSQKVRQLAMEKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPAGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDVRKESYDGAISYS
>Mature_463_residues
GKRLLDKLWEKHIVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLANRTVRRPDLTFATMDHNIPTKDVWNITDHIAKQQ
LDTLRANCKQFHISLADIGDEAQGIVHVIGPELGLTVPGKTIVCGDSHTATHGAFSALAFGIGTSEVEHVLATQTLWQRK
PKAMGIELKGTLPKGVYAKDIILHILATYGVAVGTGYVMEFYGETIRSMEMEERMTLCNMAIEGGAKAGIIAPDEKTFAY
VKGRKYAPKDFERAVEKWSELYTDSDAVYDMHISIDVSKLAPYVTWGTNPSMGIRIDEQLPAISDQNEERAFAYMGLKPK
QSAYDIPIKHVFIGSCTNSRLSDLEIAAAIVKGKKVKDGVRALVVPGSQKVRQLAMEKGLHHIFEEAGFEWREPGCSMCL
GMNPDQVPAGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDVRKESYDGAISYS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=354, Percent_Identity=26.8361581920904, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI8659555, Length=432, Percent_Identity=24.7685185185185, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=386, Percent_Identity=25.3886010362694, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=59.1397849462366, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1787531, Length=366, Percent_Identity=27.0491803278689, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87081781, Length=403, Percent_Identity=25.0620347394541, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI2367097, Length=348, Percent_Identity=24.4252873563218, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=358, Percent_Identity=31.0055865921788, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=359, Percent_Identity=30.9192200557103, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=30.8196721311475, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=428, Percent_Identity=26.1682242990654, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=55.5084745762712, Blast_Score=495, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=28.743961352657, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322261, Length=403, Percent_Identity=29.0322580645161, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=384, Percent_Identity=28.125, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=27.3657289002558, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=27.3657289002558, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.2251308900524, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28571643, Length=392, Percent_Identity=28.0612244897959, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=27.2965879265092, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137564, Length=382, Percent_Identity=25.9162303664921, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_BACCN (A7GMU3)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374446.1
- ProteinModelPortal:   A7GMU3
- SMR:   A7GMU3
- STRING:   A7GMU3
- EnsemblBacteria:   EBBACT00000039286
- GeneID:   5344477
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1125
- eggNOG:   COG0065
- GeneTree:   EBGT00050000000860
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   BCER315749:BCER98_1125-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51071; Mature: 50940

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLLDKLWEKHIVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLANRTVRRPDLTFAT
CCHHHHHHHHHHHEEEECCCCCEEEEEEEEEHHHCCCCHHHHHHHHHHCCCCCCCCEEEE
MDHNIPTKDVWNITDHIAKQQLDTLRANCKQFHISLADIGDEAQGIVHVIGPELGLTVPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCEEEEECCCCCEECCC
KTIVCGDSHTATHGAFSALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGTLPKGVYAK
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHH
DIILHILATYGVAVGTGYVMEFYGETIRSMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPKDFERAVEKWSELYTDSDAVYDMHISIDVSKLAPYVTWGTNPSMGIRIDEQ
EEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHCCCEEEECCCCCCCEEECCC
LPAISDQNEERAFAYMGLKPKQSAYDIPIKHVFIGSCTNSRLSDLEIAAAIVKGKKVKDG
CCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
VRALVVPGSQKVRQLAMEKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPAGEHCASTSNR
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDVRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GKRLLDKLWEKHIVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLANRTVRRPDLTFAT
CHHHHHHHHHHHEEEECCCCCEEEEEEEEEHHHCCCCHHHHHHHHHHCCCCCCCCEEEE
MDHNIPTKDVWNITDHIAKQQLDTLRANCKQFHISLADIGDEAQGIVHVIGPELGLTVPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCCEEEEECCCCCEECCC
KTIVCGDSHTATHGAFSALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGTLPKGVYAK
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHH
DIILHILATYGVAVGTGYVMEFYGETIRSMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPKDFERAVEKWSELYTDSDAVYDMHISIDVSKLAPYVTWGTNPSMGIRIDEQ
EEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHCCCEEEECCCCCCCEEECCC
LPAISDQNEERAFAYMGLKPKQSAYDIPIKHVFIGSCTNSRLSDLEIAAAIVKGKKVKDG
CCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
VRALVVPGSQKVRQLAMEKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPAGEHCASTSNR
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDVRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA