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The map label for this gene is leuA

Identifier: 152974927

GI number: 152974927

Start: 1235413

End: 1236933

Strand: Direct

Name: leuA

Synonym: Bcer98_1123

Alternate gene names: 152974927

Gene position: 1235413-1236933 (Clockwise)

Preceding gene: 152974926

Following gene: 152974928

Centisome position: 30.23

GC content: 39.45

Gene sequence:

>1521_bases
ATGAAACAGATTTTGTTCATGGATACGACGCTTCGTGATGGGGAACAATCACCTGGAGTCAATTTAAATGAACAAGAAAA
ATTACAAATTGCTCGGCAGCTAGAAAAGTTAGGAATCGATGTGATGGAAGCGGGATTTGCCGCGGCATCTGAAGGAGACT
TTCAATCTGTTAAACGGATTGCAGAAAGCATTCAAAATGCTTCGGTCATGAGTTTAGCAAGGGCAAAAGAAAGCGATATT
CGCAAAGCGTATGAAGCGGTAAAACGAGCTGTTTCTCCTAGACTGCATGTATTTTTAGCAACGAGTGATATTCATATGAA
ATATAAGCTCTGCATGTCAAAAGAAGACGTATTAGACAGTATTCACCGTTCTGTTATATTAGGAAAATCTTTGTTTCCTA
CTGTACAGTTTTCAGCGGAAGATGCAACGAGAACAGCGCAACCTTTTTTAGCTGAAGCTGTTGAAGTTGCTATTCGTGCA
GGAGCAGATGTCATTAATATTCCAGATACAGTTGGATATACACACCCAGAAGAATATTATTCTCTCTTTCAATATTTACA
AGAATCAGTGCCTTCCTATGAAAAAGCAATTTTCTCTTGTCATTGTCATGATGACCTTGGCATGGCGGTAGCCAATTCGT
TAGCGGCAATTGAAGGCGGGGCACTGCAGGTAGAAGGTACGATAAATGGGATTGGGGAGAGAGCAGGGAATGCAGCTTTA
GAAGAAGTAGCTGTCGCTTTGCATATTCGAAAAGATCACTATAAAACGCAGTCTTCTATAATATTGAAAGAAATAAAAGC
AACGAGTACATTAGTAAGTCGTTTAACCGGGATGATGATACCTAAAAATAAAGCAATAGTGGGAGCAAATGCGTTTGCCC
ATGAATCAGGCATTCATCAAGATGGCGTTTTAAAAGAAGTGACAACGTATGAAATTATTGCACCAGAATTAATTGGTGAA
TCACAGAATCTATTTGTTCTTGGAAAACATTCTGGACGCCATGCCTTTACGGAAAGAATGAAAGAACTCGGGTATGAGCT
TACACAAAAAGAACGAGATGCTGCTTTTGAAGCCTTCAAAGCATTAGCAGATCGTAAAAAGGAAATTACGGATGAAGATT
TACGGGCACTTATGCTTGGAGAAGCTGCACTTTTAACACAGCAGTACAATATTAAGCAGTTGCAAGTACATTTTGTGTCC
AATCATATTCAATGTGCAACGGTTGTTTTACAAGATGGGAAAGGTAATAAATATGAGGATGCTGCAACTGGAGCAGGTAG
TATCGAAGCCATTTATCATGCAATTCAAAGAATTTTAGAGATGGAATGTAAGTTAGTTGATTATCGTATTCAATCGATTA
CGCAAGGTCAGGACGCACTGGCTCATGTTCATGTGGAATTAAAAGAAGGGCCTCATCAAGTTTCAGGGTTTGGTGTTGCG
CAAGACGTATTAGAAGCATCAGCTAGAGCATACGTTCATGCTGCTGGAAAATTAAAAGCACTGTTAACGCTCGTTAAGTA
A

Upstream 100 bases:

>100_bases
AACGCAAAGGAAAATGAGCATCAAATTGAAGTAGTTGGACGGCAGTTACGTGAGATGATGCCGTTTGTAAAACCGAAAGT
AAAGACGGGGGTTAAATAAT

Downstream 100 bases:

>100_bases
CAATCAAATAAGTAAGAGAACACCTTTACTTATTTGATTCAGAAAAATCAGATAAAAAGGGGTGCTTATCATTTGGAAAA
ACGCATTGTTTGTTTAGCTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLEKLGIDVMEAGFAAASEGDFQSVKRIAESIQNASVMSLARAKESDI
RKAYEAVKRAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIHRSVILGKSLFPTVQFSAEDATRTAQPFLAEAVEVAIRA
GADVINIPDTVGYTHPEEYYSLFQYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAIEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDHYKTQSSIILKEIKATSTLVSRLTGMMIPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIAPELIGE
SQNLFVLGKHSGRHAFTERMKELGYELTQKERDAAFEAFKALADRKKEITDEDLRALMLGEAALLTQQYNIKQLQVHFVS
NHIQCATVVLQDGKGNKYEDAATGAGSIEAIYHAIQRILEMECKLVDYRIQSITQGQDALAHVHVELKEGPHQVSGFGVA
QDVLEASARAYVHAAGKLKALLTLVK

Sequences:

>Translated_506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLEKLGIDVMEAGFAAASEGDFQSVKRIAESIQNASVMSLARAKESDI
RKAYEAVKRAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIHRSVILGKSLFPTVQFSAEDATRTAQPFLAEAVEVAIRA
GADVINIPDTVGYTHPEEYYSLFQYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAIEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDHYKTQSSIILKEIKATSTLVSRLTGMMIPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIAPELIGE
SQNLFVLGKHSGRHAFTERMKELGYELTQKERDAAFEAFKALADRKKEITDEDLRALMLGEAALLTQQYNIKQLQVHFVS
NHIQCATVVLQDGKGNKYEDAATGAGSIEAIYHAIQRILEMECKLVDYRIQSITQGQDALAHVHVELKEGPHQVSGFGVA
QDVLEASARAYVHAAGKLKALLTLVK
>Mature_506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLEKLGIDVMEAGFAAASEGDFQSVKRIAESIQNASVMSLARAKESDI
RKAYEAVKRAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIHRSVILGKSLFPTVQFSAEDATRTAQPFLAEAVEVAIRA
GADVINIPDTVGYTHPEEYYSLFQYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAIEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDHYKTQSSIILKEIKATSTLVSRLTGMMIPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIAPELIGE
SQNLFVLGKHSGRHAFTERMKELGYELTQKERDAAFEAFKALADRKKEITDEDLRALMLGEAALLTQQYNIKQLQVHFVS
NHIQCATVVLQDGKGNKYEDAATGAGSIEAIYHAIQRILEMECKLVDYRIQSITQGQDALAHVHVELKEGPHQVSGFGVA
QDVLEASARAYVHAAGKLKALLTLVK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=493, Percent_Identity=46.8559837728195, Blast_Score=417, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320071, Length=374, Percent_Identity=29.4117647058824, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=369, Percent_Identity=29.2682926829268, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=532, Percent_Identity=24.0601503759398, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=317, Percent_Identity=27.4447949526814, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BACCN (A7GMU1)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374444.1
- ProteinModelPortal:   A7GMU1
- SMR:   A7GMU1
- STRING:   A7GMU1
- EnsemblBacteria:   EBBACT00000036397
- GeneID:   5344462
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1123
- eggNOG:   COG0119
- GeneTree:   EBGT00050000000890
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   BCER315749:BCER98_1123-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55545; Mature: 55545

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLEKLGIDVMEAGFAAASEGDFQSVKRI
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHH
AESIQNASVMSLARAKESDIRKAYEAVKRAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IHRSVILGKSLFPTVQFSAEDATRTAQPFLAEAVEVAIRAGADVINIPDTVGYTHPEEYY
HHHHHHHCCHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
SLFQYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAIEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDHYKTQSSIILKEIKATSTLVSRLTGMMIPKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIAPELIGESQNLFVLGKHSGRHAFTERMKELGYELTQKERDAAFEAFK
CCCHHHHHHHHHCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHH
ALADRKKEITDEDLRALMLGEAALLTQQYNIKQLQVHFVSNHIQCATVVLQDGKGNKYED
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCEEEEEEEECCCCCCCCH
AATGAGSIEAIYHAIQRILEMECKLVDYRIQSITQGQDALAHVHVELKEGPHQVSGFGVA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHH
QDVLEASARAYVHAAGKLKALLTLVK
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLEKLGIDVMEAGFAAASEGDFQSVKRI
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHH
AESIQNASVMSLARAKESDIRKAYEAVKRAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IHRSVILGKSLFPTVQFSAEDATRTAQPFLAEAVEVAIRAGADVINIPDTVGYTHPEEYY
HHHHHHHCCHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
SLFQYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAIEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDHYKTQSSIILKEIKATSTLVSRLTGMMIPKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIAPELIGESQNLFVLGKHSGRHAFTERMKELGYELTQKERDAAFEAFK
CCCHHHHHHHHHCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHH
ALADRKKEITDEDLRALMLGEAALLTQQYNIKQLQVHFVSNHIQCATVVLQDGKGNKYED
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCEEEEEEEECCCCCCCCH
AATGAGSIEAIYHAIQRILEMECKLVDYRIQSITQGQDALAHVHVELKEGPHQVSGFGVA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHH
QDVLEASARAYVHAAGKLKALLTLVK
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA