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The map label for this gene is leuB [H]

Identifier: 152974928

GI number: 152974928

Start: 1237006

End: 1238070

Strand: Direct

Name: leuB [H]

Synonym: Bcer98_1124

Alternate gene names: 152974928

Gene position: 1237006-1238070 (Clockwise)

Preceding gene: 152974927

Following gene: 152974929

Centisome position: 30.27

GC content: 42.82

Gene sequence:

>1065_bases
TTGGAAAAACGCATTGTTTGTTTAGCTGGAGACGGTGTTGGACCAGAGATTATGGAAAGTGCGAAAGACATATTGCATAT
GATTGAACGATTGTATGGTCATCAGTTTTATTTACAAGAAGAGGAATTTGGGGGAGCTGCAATCGATTTATTTGGGCAAC
CATTACCTCAAAGAACATTGGCTGCATGTTTAGCAAGCGATGCGGTCTTACTTGGAGCAGTTGGTGGACAAAGATGGGAC
AATGTGAAAGAAAGGCCGGAAAAGGGATTACTTGCTCTTCGAAAAGGGCTTGGTGTATTTGCGAATGTTCGTCCGGTAAC
AGTAGAGGCGGAGATTGCACATTTGTCTCCATTAAAGACGGCGACAGAGATTGATTTTGTTGTTGTTCGTGAATTAACAG
GCGGGATTTATTTTTCGTTTCCAAAAGAAAGGACCGAGGAAGTAGCAACTGATACATTGACATATCATCGCCGTGAGATT
GAACGCATTGTCTCCTATGCTTTTCAGCTCGCAAGTAAACGGCGCCAAAAAGTTACTTCTATTGATAAAGCGAATGTACT
CGAATCTAGTAAATTGTGGAGAACAATTACCGAAGAAGTTTCTCTTCGGTATCCAGAGATAGAGCTGGAACATATGTTGG
TTGATGCAGCAGCAATGGAATTCATTCGGAATCCAGCAAGGTTTGATGTGATTGTCACTGAGAATTTATTCGGCGATATT
TTAAGCGATGAAGCGTCTGTTTTAGCAGGATCGCTCGGGATGCTTCCATCAGCAAGTCATGCGGAGAATGGCCCATCGCT
TTATGAACCAATTCATGGCTCAGCGCCAGATATTGCAGGGAAAAATAAAGCGAATCCAATTGCGATGATGCGTTCCGTTA
AGATGATGCTTGAGCAGTCATTTGGGCTCATAAAGGAAGGAAGGGCAATTGAAGAGGCAATTACAGCAGTACTTCGATCA
AAGAAATATACAGCTGATCTTGGCGGAGAAGAAACGACAACTTCATTTACACAAGCTGTCCTTCAAGAAATGGAAGAACA
AGCGCTAGTTGGGAGAGGGCGATAA

Upstream 100 bases:

>100_bases
AAAAGCACTGTTAACGCTCGTTAAGTAACAATCAAATAAGTAAGAGAACACCTTTACTTATTTGATTCAGAAAAATCAGA
TAAAAAGGGGTGCTTATCAT

Downstream 100 bases:

>100_bases
TGGGAAAGAGACTACTAGATAAACTATGGGAAAAGCATATTGTAGCAACAAATGAAAATGGACTGGATCTATTATATATT
GATCTTCATCTTGTGCATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MEKRIVCLAGDGVGPEIMESAKDILHMIERLYGHQFYLQEEEFGGAAIDLFGQPLPQRTLAACLASDAVLLGAVGGQRWD
NVKERPEKGLLALRKGLGVFANVRPVTVEAEIAHLSPLKTATEIDFVVVRELTGGIYFSFPKERTEEVATDTLTYHRREI
ERIVSYAFQLASKRRQKVTSIDKANVLESSKLWRTITEEVSLRYPEIELEHMLVDAAAMEFIRNPARFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAENGPSLYEPIHGSAPDIAGKNKANPIAMMRSVKMMLEQSFGLIKEGRAIEEAITAVLRS
KKYTADLGGEETTTSFTQAVLQEMEEQALVGRGR

Sequences:

>Translated_354_residues
MEKRIVCLAGDGVGPEIMESAKDILHMIERLYGHQFYLQEEEFGGAAIDLFGQPLPQRTLAACLASDAVLLGAVGGQRWD
NVKERPEKGLLALRKGLGVFANVRPVTVEAEIAHLSPLKTATEIDFVVVRELTGGIYFSFPKERTEEVATDTLTYHRREI
ERIVSYAFQLASKRRQKVTSIDKANVLESSKLWRTITEEVSLRYPEIELEHMLVDAAAMEFIRNPARFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAENGPSLYEPIHGSAPDIAGKNKANPIAMMRSVKMMLEQSFGLIKEGRAIEEAITAVLRS
KKYTADLGGEETTTSFTQAVLQEMEEQALVGRGR
>Mature_354_residues
MEKRIVCLAGDGVGPEIMESAKDILHMIERLYGHQFYLQEEEFGGAAIDLFGQPLPQRTLAACLASDAVLLGAVGGQRWD
NVKERPEKGLLALRKGLGVFANVRPVTVEAEIAHLSPLKTATEIDFVVVRELTGGIYFSFPKERTEEVATDTLTYHRREI
ERIVSYAFQLASKRRQKVTSIDKANVLESSKLWRTITEEVSLRYPEIELEHMLVDAAAMEFIRNPARFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAENGPSLYEPIHGSAPDIAGKNKANPIAMMRSVKMMLEQSFGLIKEGRAIEEAITAVLRS
KKYTADLGGEETTTSFTQAVLQEMEEQALVGRGR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=32, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI28178821, Length=352, Percent_Identity=28.9772727272727, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=29.5652173913043, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758582, Length=259, Percent_Identity=31.2741312741313, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=33.5051546391753, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI28178838, Length=232, Percent_Identity=31.4655172413793, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081683, Length=334, Percent_Identity=49.1017964071856, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=35.6545961002786, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1787381, Length=375, Percent_Identity=24.5333333333333, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=362, Percent_Identity=30.939226519337, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17550882, Length=354, Percent_Identity=30.5084745762712, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=28.7709497206704, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=351, Percent_Identity=27.6353276353276, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=46.3687150837989, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.4145658263305, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=350, Percent_Identity=34.5714285714286, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=350, Percent_Identity=30, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643270, Length=360, Percent_Identity=32.5, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24643268, Length=360, Percent_Identity=32.5, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI281362242, Length=350, Percent_Identity=31.7142857142857, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24648872, Length=350, Percent_Identity=31.7142857142857, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24661184, Length=264, Percent_Identity=31.0606060606061, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI161078635, Length=224, Percent_Identity=30.8035714285714, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078639, Length=224, Percent_Identity=30.8035714285714, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078637, Length=224, Percent_Identity=30.8035714285714, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078633, Length=224, Percent_Identity=30.8035714285714, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24650122, Length=224, Percent_Identity=30.8035714285714, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20130355, Length=343, Percent_Identity=21.2827988338192, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39080; Mature: 39080

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRIVCLAGDGVGPEIMESAKDILHMIERLYGHQFYLQEEEFGGAAIDLFGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHCCCCEEEECCCCCCCHHHH
AACLASDAVLLGAVGGQRWDNVKERPEKGLLALRKGLGVFANVRPVTVEAEIAHLSPLKT
HHHHHHCCCEEECCCCCCCCCHHHCHHHHHHHHHHCCCCEECCCEEEEEEHHHHCCCCCH
ATEIDFVVVRELTGGIYFSFPKERTEEVATDTLTYHRREIERIVSYAFQLASKRRQKVTS
HHHHHHHEEEEHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTITEEVSLRYPEIELEHMLVDAAAMEFIRNPARFDVIVTENLFGDI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
LSDEASVLAGSLGMLPSASHAENGPSLYEPIHGSAPDIAGKNKANPIAMMRSVKMMLEQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FGLIKEGRAIEEAITAVLRSKKYTADLGGEETTTSFTQAVLQEMEEQALVGRGR
CCHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKRIVCLAGDGVGPEIMESAKDILHMIERLYGHQFYLQEEEFGGAAIDLFGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEHHCCCCEEEECCCCCCCHHHH
AACLASDAVLLGAVGGQRWDNVKERPEKGLLALRKGLGVFANVRPVTVEAEIAHLSPLKT
HHHHHHCCCEEECCCCCCCCCHHHCHHHHHHHHHHCCCCEECCCEEEEEEHHHHCCCCCH
ATEIDFVVVRELTGGIYFSFPKERTEEVATDTLTYHRREIERIVSYAFQLASKRRQKVTS
HHHHHHHEEEEHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTITEEVSLRYPEIELEHMLVDAAAMEFIRNPARFDVIVTENLFGDI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
LSDEASVLAGSLGMLPSASHAENGPSLYEPIHGSAPDIAGKNKANPIAMMRSVKMMLEQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
FGLIKEGRAIEEAITAVLRSKKYTADLGGEETTTSFTQAVLQEMEEQALVGRGR
CCHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]