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The map label for this gene is nrdE [H]

Identifier: 152974879

GI number: 152974879

Start: 1187862

End: 1189250

Strand: Direct

Name: nrdE [H]

Synonym: Bcer98_1075

Alternate gene names: 152974879

Gene position: 1187862-1189250 (Clockwise)

Preceding gene: 152974878

Following gene: 152974880

Centisome position: 29.06

GC content: 34.7

Gene sequence:

>1389_bases
ATGCAAAGAAAAGGTGCCGGTGCTGTATACTTAAACATTTTCCATTGGGATATTATTGAATTTCTCGATACAAAAAAAAT
AAATGCCGACGAGAAGAGCCGGATTCAGTCTCTATCAATCGGTATTATCGTTCCAAGTAAGTTCTTTGAGCTTGCTGAGA
AAAATGAACCATTCCATGTTTTCGCACCATATACAGTATATAAAGAGTACGGAAAACATTTAGATGATATTGATATTGAT
GAAATGTATGATGAGTTAATGAGCAATCCAAATGTAAAGAAAAAGTCACTAGATTTAAGTGCGCGTGATATGCTCATTAA
AATTGCAATGATTCAGCTTGAGTCTGGTTATCCATACTTAATGTTTAAATCAAATGCGAATCAGCAACATCCATTAAAGA
ATATTGGAACTGTGAAGATGTCGAACCTTTGTACAGAAATCTTTCAATTACAAGAAACATCCGAAATTAACGACTACGGT
ACAGATGACATAATCCGCCGTGATATTAACTGTAACTTAGGATCATTAAACATTGTAAACGTAATGGAGAATAAAGAAAT
TCGTGAAGCGGTACATGCAGGAATGGAAGCTTTAACAGCCGTTTCTGATATGACTGTTATTCCAAACGCACCAACTGTGA
AGAAGGCAAATGATGAGCTTCATTCGGTTGGACTTGGTGCTATGAACTTACATGGATATTTAGCGAAAAATAAAATCGCT
TATGAAAGTGCAGAAGCGAAAGAATTTGTTCGTACATTCTTTATGATGTTAAACTACTACTCGATTGAAAAGAGTATGGG
TATTGCGAAAGAAAAGGGCGAAACATTTAAAGACTTTGATAAGTCGGATTATGCAAATGGCACGTACTTTGAAAAGTATG
AAACAACAGACTATAGCCCTGTAACGGAGAAAGTACAACAGTTATTTGAAGGGATTTACATTCCAACAAAAGAAGATTGG
ACAAGTCTAAAAGAAGAGGTGCAGAAGTACGGTTTATATAACTCATATCGTTTAGCTATTGCGCCAACACAATCGATTAG
TTATGTTCAAAATGCAACTTCAAGCGTAATGCCAATTGTAAGTCAAATTGAGTCAAGAACATATGCGAATGCGACAACAT
ATTATCCAATGCCATATTTATCAAAAGATACGTTCTGGTATTATAAGTCTGCTTATGATATGAATCAGTTTAAACTGATT
GATTTAATTGCAGAGATTCAAGAGCATATTGATCAAGGAATTAGTACGATTCTTTATGTAAATAGTGACATTTCTACACG
AGAGTTAGCACGTTACTACATTTATGCACATAAAAAAGGCTTAAAGAGTCTGTATTATACAAGAACGCGTAAGTTAAGTG
TGGAAGAATGTGTAGCTTGTACGGTTTGA

Upstream 100 bases:

>100_bases
GAATTTGGTGAGAAGAAGATATGGTCTGAACTCTATAGCGATATAGAGAGGGTAGCAGAAATGACTATCCCCCATACGTT
TTAAGGTATGGAGTAACAAT

Downstream 100 bases:

>100_bases
TGTAATGATATATAACTTAAAGGATAAGACATCATGAGCAGGGTTTTATAACCCTGCTCATGCTTAGTTATAAATTTTTA
GGTTCTGATAGACTGTGATT

Product: ribonucleoside-diphosphate reductase

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDID
EMYDELMSNPNVKKKSLDLSARDMLIKIAMIQLESGYPYLMFKSNANQQHPLKNIGTVKMSNLCTEIFQLQETSEINDYG
TDDIIRRDINCNLGSLNIVNVMENKEIREAVHAGMEALTAVSDMTVIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIA
YESAEAKEFVRTFFMMLNYYSIEKSMGIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIYIPTKEDW
TSLKEEVQKYGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSAYDMNQFKLI
DLIAEIQEHIDQGISTILYVNSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Sequences:

>Translated_462_residues
MQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDID
EMYDELMSNPNVKKKSLDLSARDMLIKIAMIQLESGYPYLMFKSNANQQHPLKNIGTVKMSNLCTEIFQLQETSEINDYG
TDDIIRRDINCNLGSLNIVNVMENKEIREAVHAGMEALTAVSDMTVIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIA
YESAEAKEFVRTFFMMLNYYSIEKSMGIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIYIPTKEDW
TSLKEEVQKYGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSAYDMNQFKLI
DLIAEIQEHIDQGISTILYVNSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
>Mature_462_residues
MQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDID
EMYDELMSNPNVKKKSLDLSARDMLIKIAMIQLESGYPYLMFKSNANQQHPLKNIGTVKMSNLCTEIFQLQETSEINDYG
TDDIIRRDINCNLGSLNIVNVMENKEIREAVHAGMEALTAVSDMTVIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIA
YESAEAKEFVRTFFMMLNYYSIEKSMGIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIYIPTKEDW
TSLKEEVQKYGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSAYDMNQFKLI
DLIAEIQEHIDQGISTILYVNSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=491, Percent_Identity=26.8839103869654, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1789030, Length=468, Percent_Identity=42.7350427350427, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI2367133, Length=462, Percent_Identity=22.5108225108225, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17555242, Length=491, Percent_Identity=25.4582484725051, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322123, Length=497, Percent_Identity=24.3460764587525, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320914, Length=497, Percent_Identity=24.1448692152917, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17352481, Length=488, Percent_Identity=25.4098360655738, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 53141; Mature: 53141

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHV
CCCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEE
FAPYTVYKEYGKHLDDIDIDEMYDELMSNPNVKKKSLDLSARDMLIKIAMIQLESGYPYL
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHCCCHHHHHHEEEEEEEECCCCCEE
MFKSNANQQHPLKNIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVN
EEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEE
VMENKEIREAVHAGMEALTAVSDMTVIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIA
ECCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCHH
YESAEAKEFVRTFFMMLNYYSIEKSMGIAKEKGETFKDFDKSDYANGTYFEKYETTDYSP
HCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCH
VTEKVQQLFEGIYIPTKEDWTSLKEEVQKYGLYNSYRLAIAPTQSISYVQNATSSVMPIV
HHHHHHHHHHCEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
SQIESRTYANATTYYPMPYLSKDTFWYYKSAYDMNQFKLIDLIAEIQEHIDQGISTILYV
HHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
NSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHV
CCCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEE
FAPYTVYKEYGKHLDDIDIDEMYDELMSNPNVKKKSLDLSARDMLIKIAMIQLESGYPYL
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHCCCHHHHHHEEEEEEEECCCCCEE
MFKSNANQQHPLKNIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVN
EEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEE
VMENKEIREAVHAGMEALTAVSDMTVIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIA
ECCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCHH
YESAEAKEFVRTFFMMLNYYSIEKSMGIAKEKGETFKDFDKSDYANGTYFEKYETTDYSP
HCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCH
VTEKVQQLFEGIYIPTKEDWTSLKEEVQKYGLYNSYRLAIAPTQSISYVQNATSSVMPIV
HHHHHHHHHHCEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
SQIESRTYANATTYYPMPYLSKDTFWYYKSAYDMNQFKLIDLIAEIQEHIDQGISTILYV
HHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
NSDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]