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The map label for this gene is potD-A [H]

Identifier: 152974814

GI number: 152974814

Start: 1125541

End: 1126593

Strand: Direct

Name: potD-A [H]

Synonym: Bcer98_1010

Alternate gene names: 152974814

Gene position: 1125541-1126593 (Clockwise)

Preceding gene: 152974813

Following gene: 152974815

Centisome position: 27.54

GC content: 35.23

Gene sequence:

>1053_bases
ATGAAATTAATGAAGACGATAGCCGGCGCAGCCATTAGTTTTAGCCTTGTAGCAGGTGTACTTGCAGGATGCGGCGGTGA
AAAAAAAGAAGAGCTTAACATTTATAGCTGGGCGGATAACTTTGATGAGCAAGTATTAAGGGATTTTGAGAAAAAATACA
ATGTCAAGATTAACTATGACAAGTATGCAAGTAACGAAGAAATGTTTGCGAAGTTACAAGCTGGTGGTGCGAAATATGAT
TTAATTCAGCCATCTGATTACATGGTGAAAACAATGGCCGAAATGGATTTATTAGAGCCATTAAATAAGAAAAACATTCC
GAATGTAGAAAATCTTGTTTCTAACTTTAAAACGCCAGCATTTGATCCGGAGAATAAATACTCTCTTGTATACACTTGGG
GTGTAACAGGAATTGCTTACAATAAAAAGTATGTAAAAGAAACGCCAACAAGCTGGAATGATTTATGGAATGAGAAGTAT
AAAGGACGCGTTACTTTATTAAATGATTCTCGTGAAGTATTAGGTATGGCGCTTAAGAAAAATGGATTTTCCAATAGTAC
AAAAGATGATGCCCAATTAAAAACAGCGGCAACGGACTTACAGAAGTTACTTCCAAACCTACTCGCATTTGACACAGATA
ACATTAAGCAAAAATTCATTACAGAAGATACATGGATTGGAACAGTATGGTCTGGAGATGCAGCGTTTATTGCAAAAGAT
AATAAAGATGTAGGGTATGTCGTTCCAAAAGAAGGCGGTACAATTTGGGCGGATACATTAGCAATTCCAAAAGGTGCGAA
ACATAAAGAACTTGCTGAGAAGTTTATGAACTATTTAATGGATGAAAAAGTAAGTGTGAAAAACTATGAATCCATTGGAT
ATAGTAATCCAAACGAAAAAGCTTGGCCACTTCATAGTAAAGAATACCGCGATAACAAAATGATTTTCTTGTCACAAGAT
GAGTTAAATCGTACAGAATGGCTTGTTGATGTAGATGATAAATTAAAAGAGTATGATCGTTACTGGACAGAGTTAAAAAC
AAAAGGAAAATAA

Upstream 100 bases:

>100_bases
TGATTGCATCAGAAATATTTCGTAATAAAGGAGCGGATGGAGAAGAAAACTCCGGAGGGCATCTTCCTTTATAAAAACAG
ACAGAGGAGGTCGTGAATCG

Downstream 100 bases:

>100_bases
AGAATAAAAATGCGGTTTCTATATAGAAATCGCATTTTTTCATAAGTAGGAGGAGAAAGTTTGAAGAAAAAATTGCATCA
ATATGAATTACTATGTATGG

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKLMKTIAGAAISFSLVAGVLAGCGGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMFAKLQAGGAKYD
LIQPSDYMVKTMAEMDLLEPLNKKNIPNVENLVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKETPTSWNDLWNEKY
KGRVTLLNDSREVLGMALKKNGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDTWIGTVWSGDAAFIAKD
NKDVGYVVPKEGGTIWADTLAIPKGAKHKELAEKFMNYLMDEKVSVKNYESIGYSNPNEKAWPLHSKEYRDNKMIFLSQD
ELNRTEWLVDVDDKLKEYDRYWTELKTKGK

Sequences:

>Translated_350_residues
MKLMKTIAGAAISFSLVAGVLAGCGGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMFAKLQAGGAKYD
LIQPSDYMVKTMAEMDLLEPLNKKNIPNVENLVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKETPTSWNDLWNEKY
KGRVTLLNDSREVLGMALKKNGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDTWIGTVWSGDAAFIAKD
NKDVGYVVPKEGGTIWADTLAIPKGAKHKELAEKFMNYLMDEKVSVKNYESIGYSNPNEKAWPLHSKEYRDNKMIFLSQD
ELNRTEWLVDVDDKLKEYDRYWTELKTKGK
>Mature_350_residues
MKLMKTIAGAAISFSLVAGVLAGCGGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMFAKLQAGGAKYD
LIQPSDYMVKTMAEMDLLEPLNKKNIPNVENLVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKETPTSWNDLWNEKY
KGRVTLLNDSREVLGMALKKNGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDTWIGTVWSGDAAFIAKD
NKDVGYVVPKEGGTIWADTLAIPKGAKHKELAEKFMNYLMDEKVSVKNYESIGYSNPNEKAWPLHSKEYRDNKMIFLSQD
ELNRTEWLVDVDDKLKEYDRYWTELKTKGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=329, Percent_Identity=35.5623100303951, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787078, Length=366, Percent_Identity=31.6939890710383, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787711, Length=257, Percent_Identity=27.6264591439689, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40039; Mature: 40039

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMKTIAGAAISFSLVAGVLAGCGGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEEEECCCCHHHHHHHHHHHHCCEEEEEC
KYASNEEMFAKLQAGGAKYDLIQPSDYMVKTMAEMDLLEPLNKKNIPNVENLVSNFKTPA
CCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCC
FDPENKYSLVYTWGVTGIAYNKKYVKETPTSWNDLWNEKYKGRVTLLNDSREVLGMALKK
CCCCCCEEEEEEECCCEEEECCHHHHCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHC
NGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDTWIGTVWSGDAAFIAKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCEEEEEEC
NKDVGYVVPKEGGTIWADTLAIPKGAKHKELAEKFMNYLMDEKVSVKNYESIGYSNPNEK
CCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
AWPLHSKEYRDNKMIFLSQDELNRTEWLVDVDDKLKEYDRYWTELKTKGK
CCCCCCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLMKTIAGAAISFSLVAGVLAGCGGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEEEECCCCHHHHHHHHHHHHCCEEEEEC
KYASNEEMFAKLQAGGAKYDLIQPSDYMVKTMAEMDLLEPLNKKNIPNVENLVSNFKTPA
CCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCC
FDPENKYSLVYTWGVTGIAYNKKYVKETPTSWNDLWNEKYKGRVTLLNDSREVLGMALKK
CCCCCCEEEEEEECCCEEEECCHHHHCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHC
NGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDTWIGTVWSGDAAFIAKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCEEEEEEC
NKDVGYVVPKEGGTIWADTLAIPKGAKHKELAEKFMNYLMDEKVSVKNYESIGYSNPNEK
CCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
AWPLHSKEYRDNKMIFLSQDELNRTEWLVDVDDKLKEYDRYWTELKTKGK
CCCCCCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]