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The map label for this gene is potB [H]

Identifier: 152974812

GI number: 152974812

Start: 1123862

End: 1124707

Strand: Direct

Name: potB [H]

Synonym: Bcer98_1008

Alternate gene names: 152974812

Gene position: 1123862-1124707 (Clockwise)

Preceding gene: 152974811

Following gene: 152974813

Centisome position: 27.5

GC content: 35.46

Gene sequence:

>846_bases
TTGAAAAAAGGGAAACTACTCGCATTACCTACAGTTGCATGGCTCTTAATTTTCTTTTTATTTCCGCTCTTATTTGTATT
AGCATTTGCATTCTTCCAGCGCGGTGCGTACGGCACAGTGGAAATGCAATTTACATTTGATAATATAGTACGCGTCTTTG
ATTCGCTATATATGAGTACATTATGGGAGACAGTGAAGATTGCTGTTATTACAACGGTGCTTTGCTTACTAATTGGATAT
CCATTTGCATATACAATCACGATTGTAGATCGTAAATATCGTTCTATTCTTTTATTATTGGCAACAATTCCGTTTTGGAT
TAACTTCCTTGTCCGTTCATACGCATGGATTGTTATTTTACGTTCACAAGGTCTTGTGAATACAGTATTACTAAAATTAG
GCATTATTCATGAACCATTAAATTTATTATATAATACACCATCTGTAATATTAGGTATGGTTTATTCTTTATTACCATTT
ATGATTTTACCGGTGTATGCAGCGATTGAACAGTTTGATAAACGCAAGTTAGAAGCAGCGTATGACTTAGGCGCAACGCC
AATTAAGGCATTTTGGCATGTGACACTTCCAATGACAATGTCAGGAGTTGCAACGGGTTCCATTTTAGTGTTTGTTTCAT
CTATTGGTATGTTTGTCGTATCGGATGTAATGGGTGGATCGAAAGTAGCATTAATTGGAAACGTAATTCAAAACCAATTT
TTAGGTGCACGTGATTGGCCATTTGGTTCTGCATTATCGATTATTGTCGTCCTATTCTCTGTTCTATTAATTTACTTATA
TTATCGTGCGACGAAAGTATATAAATATGGAAACGGAGGGGAATAG

Upstream 100 bases:

>100_bases
AATTATTAATGACATATCAAACTGCAGAAGAAGCGGCGCAGTGGAGTATCGGAGATAATGTATACGTAGGCTGGGAGCAA
GAGGATGAGGTGACCTTAAG

Downstream 100 bases:

>100_bases
TACGCGATGAAAAAGTTTTTAGTTTCTTACTCTTGGTTAATTTTATTGTTCCTATATTTTCCAATGATGGTTTTAATGGT
CTATTCTTTTAATGATTCGC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKKGKLLALPTVAWLLIFFLFPLLFVLAFAFFQRGAYGTVEMQFTFDNIVRVFDSLYMSTLWETVKIAVITTVLCLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTVLLKLGIIHEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQFDKRKLEAAYDLGATPIKAFWHVTLPMTMSGVATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYGNGGE

Sequences:

>Translated_281_residues
MKKGKLLALPTVAWLLIFFLFPLLFVLAFAFFQRGAYGTVEMQFTFDNIVRVFDSLYMSTLWETVKIAVITTVLCLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTVLLKLGIIHEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQFDKRKLEAAYDLGATPIKAFWHVTLPMTMSGVATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYGNGGE
>Mature_281_residues
MKKGKLLALPTVAWLLIFFLFPLLFVLAFAFFQRGAYGTVEMQFTFDNIVRVFDSLYMSTLWETVKIAVITTVLCLLIGY
PFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVILRSQGLVNTVLLKLGIIHEPLNLLYNTPSVILGMVYSLLPF
MILPVYAAIEQFDKRKLEAAYDLGATPIKAFWHVTLPMTMSGVATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYGNGGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=262, Percent_Identity=43.1297709923664, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1787080, Length=238, Percent_Identity=39.0756302521008, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=30.4526748971193, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31580; Mature: 31580

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGKLLALPTVAWLLIFFLFPLLFVLAFAFFQRGAYGTVEMQFTFDNIVRVFDSLYMST
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
LWETVKIAVITTVLCLLIGYPFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVIL
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RSQGLVNTVLLKLGIIHEPLNLLYNTPSVILGMVYSLLPFMILPVYAAIEQFDKRKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDLGATPIKAFWHVTLPMTMSGVATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
HCCCCCHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYGNGGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MKKGKLLALPTVAWLLIFFLFPLLFVLAFAFFQRGAYGTVEMQFTFDNIVRVFDSLYMST
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
LWETVKIAVITTVLCLLIGYPFAYTITIVDRKYRSILLLLATIPFWINFLVRSYAWIVIL
HHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RSQGLVNTVLLKLGIIHEPLNLLYNTPSVILGMVYSLLPFMILPVYAAIEQFDKRKLEAA
ECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDLGATPIKAFWHVTLPMTMSGVATGSILVFVSSIGMFVVSDVMGGSKVALIGNVIQNQF
HCCCCCHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGARDWPFGSALSIIVVLFSVLLIYLYYRATKVYKYGNGGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]