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The map label for this gene is rfbB [H]

Identifier: 152974749

GI number: 152974749

Start: 1053827

End: 1054798

Strand: Direct

Name: rfbB [H]

Synonym: Bcer98_0939

Alternate gene names: 152974749

Gene position: 1053827-1054798 (Clockwise)

Preceding gene: 152974748

Following gene: 152974750

Centisome position: 25.78

GC content: 36.52

Gene sequence:

>972_bases
ATGAATATATTCGTAACTGGTGGAGCAGGTTTTATTGGAAGTAACTTTATTCGTTATATATTACGGACGCATCAGACATA
TCGTATCATTAACTACGATCTTTTAACATATAGTGGGAATCTTGACAATTTGCAATCTGTTCATCAAGATTCTCGTTATT
TTTTTGTACAAGGAGATATTCAAAATCGAGAACTTGCCCAAAAAGTAATTTTGCAACATCGTATCGATTGTATTATTAAT
TTTGCAGCAGAATCACATGTAGATCGAAGTATTGAAAACTCAACTCCCTTTTTCAATACAAACGTAATGGGAACAGTCGC
ATTGTTAGAAATAGTGAAAGAGTATCCGCATATAAGAATGCTTCAAGTTTCAACAGATGAAGTGTATGGGTCGTTAGGAA
ATAAGGGGCTTTTCACAGAAACGACACCACTAGCGCCAAGTAGCCCATATTCTTCAAGTAAAGCAAGTGCAGATATGATT
GCTCTTTCTTATTTCAAAACATATGGAACGCACGTCTCAATTACACGTTGTTCAAACAATTATGGCCCGTATCAATATCC
TGAAAAGTTAATTCCGCTTATGATTATTCAAGCGTTAAAAGGAAAACAGTTGCCTGTATATGGAGATGGAAAAAATGTTC
GTGATTGGCTTCATGTAGAAGATCATTGCCGAGCTCTTGATGCGGTTATGCATAGAGGAAAAGCAGGAGAAGTATATAAT
ATTGGAGGGAATCAGGAAAGAACAAATATAGAAGTGGTGCGAAGTATACTACAGCAACTTGGAAAGTCAGAAACAGCCAT
TGAATTTGTAGCAGACCGCCTTGGACATGATAGGCGTTATGCAATTGATGCAAGTAAAATAAAGAAGGAACTAGGATGGA
GTCCGACTTATACATTTGAAGAAGGTTTAAGGGAAACGATTGAGTGGTATTGTGAGAATGAATTATGGTGGAGCCGACTA
CAACAAAAATAA

Upstream 100 bases:

>100_bases
CGATTACATGGCCGGTCAGTGAACCGATTCTTTCAGAAAAAGATAAAGCGTTGCCTACATTTGACCAATATCATGATTGT
TTTAAGTAGAGGTGTTGGAT

Downstream 100 bases:

>100_bases
GGAGCGACTGTATGAAGAAGATCAAAGTCTGTATTACAGGAGCTAACGGTCAGTTAGGGAAAGAATTGCAAGAAGTATTT
ACGCTTGATAAATATGAAGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNIFVTGGAGFIGSNFIRYILRTHQTYRIINYDLLTYSGNLDNLQSVHQDSRYFFVQGDIQNRELAQKVILQHRIDCIIN
FAAESHVDRSIENSTPFFNTNVMGTVALLEIVKEYPHIRMLQVSTDEVYGSLGNKGLFTETTPLAPSSPYSSSKASADMI
ALSYFKTYGTHVSITRCSNNYGPYQYPEKLIPLMIIQALKGKQLPVYGDGKNVRDWLHVEDHCRALDAVMHRGKAGEVYN
IGGNQERTNIEVVRSILQQLGKSETAIEFVADRLGHDRRYAIDASKIKKELGWSPTYTFEEGLRETIEWYCENELWWSRL
QQK

Sequences:

>Translated_323_residues
MNIFVTGGAGFIGSNFIRYILRTHQTYRIINYDLLTYSGNLDNLQSVHQDSRYFFVQGDIQNRELAQKVILQHRIDCIIN
FAAESHVDRSIENSTPFFNTNVMGTVALLEIVKEYPHIRMLQVSTDEVYGSLGNKGLFTETTPLAPSSPYSSSKASADMI
ALSYFKTYGTHVSITRCSNNYGPYQYPEKLIPLMIIQALKGKQLPVYGDGKNVRDWLHVEDHCRALDAVMHRGKAGEVYN
IGGNQERTNIEVVRSILQQLGKSETAIEFVADRLGHDRRYAIDASKIKKELGWSPTYTFEEGLRETIEWYCENELWWSRL
QQK
>Mature_323_residues
MNIFVTGGAGFIGSNFIRYILRTHQTYRIINYDLLTYSGNLDNLQSVHQDSRYFFVQGDIQNRELAQKVILQHRIDCIIN
FAAESHVDRSIENSTPFFNTNVMGTVALLEIVKEYPHIRMLQVSTDEVYGSLGNKGLFTETTPLAPSSPYSSSKASADMI
ALSYFKTYGTHVSITRCSNNYGPYQYPEKLIPLMIIQALKGKQLPVYGDGKNVRDWLHVEDHCRALDAVMHRGKAGEVYN
IGGNQERTNIEVVRSILQQLGKSETAIEFVADRLGHDRRYAIDASKIKKELGWSPTYTFEEGLRETIEWYCENELWWSRL
QQK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=37.7300613496933, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI42516563, Length=315, Percent_Identity=27.3015873015873, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=24.7058823529412, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=24.7058823529412, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=24.7058823529412, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4504031, Length=335, Percent_Identity=23.5820895522388, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=51.4705882352941, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=49.5601173020528, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1786974, Length=341, Percent_Identity=28.7390029325513, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788366, Length=346, Percent_Identity=24.5664739884393, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=36.6071428571429, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=317, Percent_Identity=33.7539432176656, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17539532, Length=315, Percent_Identity=23.8095238095238, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=25.8522727272727, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=25.4237288135593, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=349, Percent_Identity=24.9283667621776, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356223, Length=328, Percent_Identity=26.219512195122, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19923002, Length=346, Percent_Identity=27.7456647398844, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24158427, Length=330, Percent_Identity=23.6363636363636, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36952; Mature: 36952

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFVTGGAGFIGSNFIRYILRTHQTYRIINYDLLTYSGNLDNLQSVHQDSRYFFVQGDI
CEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHCCCCEEEEECCC
QNRELAQKVILQHRIDCIINFAAESHVDRSIENSTPFFNTNVMGTVALLEIVKEYPHIRM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCEEE
LQVSTDEVYGSLGNKGLFTETTPLAPSSPYSSSKASADMIALSYFKTYGTHVSITRCSNN
EEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCC
YGPYQYPEKLIPLMIIQALKGKQLPVYGDGKNVRDWLHVEDHCRALDAVMHRGKAGEVYN
CCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
IGGNQERTNIEVVRSILQQLGKSETAIEFVADRLGHDRRYAIDASKIKKELGWSPTYTFE
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCHHH
EGLRETIEWYCENELWWSRLQQK
HHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MNIFVTGGAGFIGSNFIRYILRTHQTYRIINYDLLTYSGNLDNLQSVHQDSRYFFVQGDI
CEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHCCCCEEEEECCC
QNRELAQKVILQHRIDCIINFAAESHVDRSIENSTPFFNTNVMGTVALLEIVKEYPHIRM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCEEE
LQVSTDEVYGSLGNKGLFTETTPLAPSSPYSSSKASADMIALSYFKTYGTHVSITRCSNN
EEECHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCC
YGPYQYPEKLIPLMIIQALKGKQLPVYGDGKNVRDWLHVEDHCRALDAVMHRGKAGEVYN
CCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
IGGNQERTNIEVVRSILQQLGKSETAIEFVADRLGHDRRYAIDASKIKKELGWSPTYTFE
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCHHH
EGLRETIEWYCENELWWSRLQQK
HHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]