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The map label for this gene is spsK [H]

Identifier: 152974750

GI number: 152974750

Start: 1054810

End: 1055661

Strand: Direct

Name: spsK [H]

Synonym: Bcer98_0940

Alternate gene names: 152974750

Gene position: 1054810-1055661 (Clockwise)

Preceding gene: 152974749

Following gene: 152974757

Centisome position: 25.81

GC content: 36.85

Gene sequence:

>852_bases
ATGAAGAAGATCAAAGTCTGTATTACAGGAGCTAACGGTCAGTTAGGGAAAGAATTGCAAGAAGTATTTACGCTTGATAA
ATATGAAGTGCATCCCTTAGGAAAAGATGTTTTAGATGTAACGAAAATGGAAGACGTGATGAGGCTGATGCAACAACTGC
AGCCAGATATCGTTATTCATGCTGCTGCTTATACAAAAGTGGATAAAGCGGAAGACGAGCAAGCATTGGCTTTTTTAGTC
AATGCAATTGGAACAAGAAATATCGCAGTTGCTGCACAAGAGTACAATGCAAAGCTTGTTTATATTAGTACAGATTATGT
GTTTTCAGGTGATCAAAAAGAAGGATACCATGAATTTCACGCTACAGCACCTATCAATATATATGGTCATTCAAAGTTAG
CGGGGGAACAATTTGTCCAAAGTCTACATCACAGGTACTTTATTGTTCGGACTTCCTGGCTATATGGAAAGTATGGGCAT
AATTTTGTGAAGACGATTCTTCGCTTAGCGGATGAGAGAGAGGAAATATCAATCGTTTCCGATCAAATTGGTTCACCAAC
ATATGCTGGGGATTTAGCTCGTATGATTGAAAAATTAGTAGAAACGAATTTATATGGTATCTATCATGTGTGTAATAGCG
GATCATGTTCTTGGTATGAATTTGCAAAGAACGCATTACAAATAATGCGAAAAGATGTGAAAGTTCATCCTATAACGACA
AAAGACTATGGAGCAAAAGCAAAGAGACCTCAGTACTCTATTTTGAAAAATCATATGATAGAATTAAATGGTTTTCCTAT
TTTGAGAGGATGGAATGAGGCGTTGGCACAATGTATAGAGAGAATAAAGTGA

Upstream 100 bases:

>100_bases
CGACTTATACATTTGAAGAAGGTTTAAGGGAAACGATTGAGTGGTATTGTGAGAATGAATTATGGTGGAGCCGACTACAA
CAAAAATAAGGAGCGACTGT

Downstream 100 bases:

>100_bases
AACTTTAATCAGTGGGGTTTTCTTCATCCCCCACTGATTATGAGTCCTCACCAATCAGGCTGTTACGGGTAGCTCTACTC
CCACCTATCTTCCTTGCTTC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKIKVCITGANGQLGKELQEVFTLDKYEVHPLGKDVLDVTKMEDVMRLMQQLQPDIVIHAAAYTKVDKAEDEQALAFLV
NAIGTRNIAVAAQEYNAKLVYISTDYVFSGDQKEGYHEFHATAPINIYGHSKLAGEQFVQSLHHRYFIVRTSWLYGKYGH
NFVKTILRLADEREEISIVSDQIGSPTYAGDLARMIEKLVETNLYGIYHVCNSGSCSWYEFAKNALQIMRKDVKVHPITT
KDYGAKAKRPQYSILKNHMIELNGFPILRGWNEALAQCIERIK

Sequences:

>Translated_283_residues
MKKIKVCITGANGQLGKELQEVFTLDKYEVHPLGKDVLDVTKMEDVMRLMQQLQPDIVIHAAAYTKVDKAEDEQALAFLV
NAIGTRNIAVAAQEYNAKLVYISTDYVFSGDQKEGYHEFHATAPINIYGHSKLAGEQFVQSLHHRYFIVRTSWLYGKYGH
NFVKTILRLADEREEISIVSDQIGSPTYAGDLARMIEKLVETNLYGIYHVCNSGSCSWYEFAKNALQIMRKDVKVHPITT
KDYGAKAKRPQYSILKNHMIELNGFPILRGWNEALAQCIERIK
>Mature_283_residues
MKKIKVCITGANGQLGKELQEVFTLDKYEVHPLGKDVLDVTKMEDVMRLMQQLQPDIVIHAAAYTKVDKAEDEQALAFLV
NAIGTRNIAVAAQEYNAKLVYISTDYVFSGDQKEGYHEFHATAPINIYGHSKLAGEQFVQSLHHRYFIVRTSWLYGKYGH
NFVKTILRLADEREEISIVSDQIGSPTYAGDLARMIEKLVETNLYGIYHVCNSGSCSWYEFAKNALQIMRKDVKVHPITT
KDYGAKAKRPQYSILKNHMIELNGFPILRGWNEALAQCIERIK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=297, Percent_Identity=25.5892255892256, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI33519455, Length=297, Percent_Identity=25.5892255892256, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788352, Length=284, Percent_Identity=37.6760563380282, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32205; Mature: 32205

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKVCITGANGQLGKELQEVFTLDKYEVHPLGKDVLDVTKMEDVMRLMQQLQPDIVIH
CCEEEEEEECCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
AAAYTKVDKAEDEQALAFLVNAIGTRNIAVAAQEYNAKLVYISTDYVFSGDQKEGYHEFH
EHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECEEEECCCCCCCHHHC
ATAPINIYGHSKLAGEQFVQSLHHRYFIVRTSWLYGKYGHNFVKTILRLADEREEISIVS
CCCCEEEECCCHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
DQIGSPTYAGDLARMIEKLVETNLYGIYHVCNSGSCSWYEFAKNALQIMRKDVKVHPITT
HHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEECCC
KDYGAKAKRPQYSILKNHMIELNGFPILRGWNEALAQCIERIK
CCCCCCCCCCHHHHHHCCEEEECCCEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIKVCITGANGQLGKELQEVFTLDKYEVHPLGKDVLDVTKMEDVMRLMQQLQPDIVIH
CCEEEEEEECCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
AAAYTKVDKAEDEQALAFLVNAIGTRNIAVAAQEYNAKLVYISTDYVFSGDQKEGYHEFH
EHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECEEEECCCCCCCHHHC
ATAPINIYGHSKLAGEQFVQSLHHRYFIVRTSWLYGKYGHNFVKTILRLADEREEISIVS
CCCCEEEECCCHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
DQIGSPTYAGDLARMIEKLVETNLYGIYHVCNSGSCSWYEFAKNALQIMRKDVKVHPITT
HHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEECCC
KDYGAKAKRPQYSILKNHMIELNGFPILRGWNEALAQCIERIK
CCCCCCCCCCHHHHHHCCEEEECCCEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]