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The map label for this gene is cls [H]

Identifier: 152974719

GI number: 152974719

Start: 1025156

End: 1026700

Strand: Direct

Name: cls [H]

Synonym: Bcer98_0907

Alternate gene names: 152974719

Gene position: 1025156-1026700 (Clockwise)

Preceding gene: 152974718

Following gene: 152974720

Centisome position: 25.08

GC content: 35.6

Gene sequence:

>1545_bases
ATGAAAAATACATTAAAACTACTTTTCTTTTTTGTAGGATTATTTATAGCGTTTGTTTCGTTACGTATGGTTATTGATGT
AGCTTTTTATTCAGATGTAATAGGCATACGAAATATTTCGTTTTTAGGTATTACAAGTATTTTATTTACCATTTCAGCAT
TTTTAATTGGTTGTGTAATTTTCTTAGAAAATAGACACCCTTCTAAAACATTAACATGGCTCATTGTACTAGGCATTTTC
CCTGTCATTGGCTTTTTTGCTTATTTATTATTTGGACAAAATTTTCGAAGAAAGCGCATGTTCCAAAAGAAGGCACTTTT
GGATGAACAGGCTTTCTTACAGTACAAGGGACATGAAGATTATAGAGAGCGTATTTTACAAAAACATAAACATCAGGAGC
TATTATTTCGTTTAGCTGATCGCCTTGGGGCACTGAATATTTCTTTTCAGACGGAAACAAAGGTGTTAACGAATGGTGAA
GAAACGTTTCAAGCCATTTTGCAAGGCTTACAAAAGGCGAAGCATCATATTCATATGGAATACTATATTGTGCGCGATGA
CAAACTTGGGACAAAGATTAAAGATATTTTAATCCAAAAGGTGAAAGAAGGAGTTGTTGTTCGCTTTTTATATGATGCGG
TAGGTAGTTTTAAACTTTCAAATGCCTATATTGAAGAATTGAATAAGGCGGGAGTGGAGATGATCCCGTTCTTCCCAGTC
CGTTTTCCCATTTTAAATGATAAAATTAATTATCGAAATCATCGAAAAATCGTGGTGATTGATGGGAATGAAGGGTTTGT
AGGGGGACTTAATATTGGCGATGAATATTTAGGAGAAAATAAATATTTTGGATTTTGGCGAGATACGCATTTATATTTAC
GAGGCGAAGCTGTTCAAAGTTTACAATTGATTTTTTTACAAGATTGGTTTTATATGACAGGCGAAGCTGTACTAGCTCCT
GAATATTTACAAGCACAATCTGTAGAAGGAGATCATTGGGGGGGCGTACAGCTTGTAGCAGGAGGGCCAGATAACAAATG
GGAAACGATTAAACATCTATATTTTGCGATGATTGCCTCTGCGAGAAAATCGATTTGGATTGCTACACCGTACTTTATTC
CCGATGATGATATTTTGTCGGCGCTGAAAGTTGCCGCGCTTGCAGGCATTGATGTGCGTTTATTAATGCCGAGCAAACCG
GATAAGCGCACAGTCTTTTATGCATCGAGATCGTATTTCCCGGAGTTATTAGATGCAGGTGTGAAAATATATGAATATGA
AAAAGGATTTCTGCATAGTAAAGTTGTAATTGTTGATTCTGACTTGGCTTCCATTGGGACAGCAAATATGGACATGAGAA
GTTTTCATTTGAATTTTGAAGTAAATGCCTTTTTATATGATACAGGGAGTATTCGAAAACTTGTACAAGATTTTGAAGAG
GACTTACAAGTGTCAAGCGAAATTCATGGTGATCATTTTCATAAACGACGCCTGCATAAACGGATTGTTGAATCAACATA
TCGCTTACTATCGCCATTATTATAA

Upstream 100 bases:

>100_bases
GGCCGATTTCAATTATATGAAATCGGCTTTTTATATGATGGGTATAATAGGGAAAGTATACTTTCGCTAGTAGAAAGCTT
TTTTAGTAAGGGGGGTAGAA

Downstream 100 bases:

>100_bases
AAAGGTGTTCTGAAGAGAACATCTTTTTTTTCTTTAAGGTAGACAGGAATGAGGACGTATGTTGCAGAATGTAACGAAAT
GTAGAAAGGATGTGGCGCTA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKNTLKLLFFFVGLFIAFVSLRMVIDVAFYSDVIGIRNISFLGITSILFTISAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVIGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYRERILQKHKHQELLFRLADRLGALNISFQTETKVLTNGE
ETFQAILQGLQKAKHHIHMEYYIVRDDKLGTKIKDILIQKVKEGVVVRFLYDAVGSFKLSNAYIEELNKAGVEMIPFFPV
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGENKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAQSVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTGSIRKLVQDFEE
DLQVSSEIHGDHFHKRRLHKRIVESTYRLLSPLL

Sequences:

>Translated_514_residues
MKNTLKLLFFFVGLFIAFVSLRMVIDVAFYSDVIGIRNISFLGITSILFTISAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVIGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYRERILQKHKHQELLFRLADRLGALNISFQTETKVLTNGE
ETFQAILQGLQKAKHHIHMEYYIVRDDKLGTKIKDILIQKVKEGVVVRFLYDAVGSFKLSNAYIEELNKAGVEMIPFFPV
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGENKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAQSVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTGSIRKLVQDFEE
DLQVSSEIHGDHFHKRRLHKRIVESTYRLLSPLL
>Mature_514_residues
MKNTLKLLFFFVGLFIAFVSLRMVIDVAFYSDVIGIRNISFLGITSILFTISAFLIGCVIFLENRHPSKTLTWLIVLGIF
PVIGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHEDYRERILQKHKHQELLFRLADRLGALNISFQTETKVLTNGE
ETFQAILQGLQKAKHHIHMEYYIVRDDKLGTKIKDILIQKVKEGVVVRFLYDAVGSFKLSNAYIEELNKAGVEMIPFFPV
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGENKYFGFWRDTHLYLRGEAVQSLQLIFLQDWFYMTGEAVLAP
EYLQAQSVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIASARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKP
DKRTVFYASRSYFPELLDAGVKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTGSIRKLVQDFEE
DLQVSSEIHGDHFHKRRLHKRIVESTYRLLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=488, Percent_Identity=28.8934426229508, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1787007, Length=323, Percent_Identity=29.7213622291022, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59276; Mature: 59276

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTLKLLFFFVGLFIAFVSLRMVIDVAFYSDVIGIRNISFLGITSILFTISAFLIGCVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVIGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCHH
YRERILQKHKHQELLFRLADRLGALNISFQTETKVLTNGEETFQAILQGLQKAKHHIHME
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTKIKDILIQKVKEGVVVRFLYDAVGSFKLSNAYIEELNKAGVEMIPFFPV
EEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEHHHHHHHHHHCCCEEEECCEE
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGENKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCEEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAQSVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTGSIRKLVQDFEE
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLQVSSEIHGDHFHKRRLHKRIVESTYRLLSPLL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNTLKLLFFFVGLFIAFVSLRMVIDVAFYSDVIGIRNISFLGITSILFTISAFLIGCVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FLENRHPSKTLTWLIVLGIFPVIGFFAYLLFGQNFRRKRMFQKKALLDEQAFLQYKGHED
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCHH
YRERILQKHKHQELLFRLADRLGALNISFQTETKVLTNGEETFQAILQGLQKAKHHIHME
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHEEEEE
YYIVRDDKLGTKIKDILIQKVKEGVVVRFLYDAVGSFKLSNAYIEELNKAGVEMIPFFPV
EEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEHHHHHHHHHHCCCEEEECCEE
RFPILNDKINYRNHRKIVVIDGNEGFVGGLNIGDEYLGENKYFGFWRDTHLYLRGEAVQS
EEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCEEEEEECEEEEEECCHHHH
LQLIFLQDWFYMTGEAVLAPEYLQAQSVEGDHWGGVQLVAGGPDNKWETIKHLYFAMIAS
HHHHHHHHHHHHCCCEEECCHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
ARKSIWIATPYFIPDDDILSALKVAALAGIDVRLLMPSKPDKRTVFYASRSYFPELLDAG
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECHHCCHHHHHCC
VKIYEYEKGFLHSKVVIVDSDLASIGTANMDMRSFHLNFEVNAFLYDTGSIRKLVQDFEE
CEEEEECCCCCEEEEEEEECCHHHCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHHH
DLQVSSEIHGDHFHKRRLHKRIVESTYRLLSPLL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]