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The map label for this gene is asnO [H]

Identifier: 152974685

GI number: 152974685

Start: 988745

End: 990601

Strand: Direct

Name: asnO [H]

Synonym: Bcer98_0872

Alternate gene names: 152974685

Gene position: 988745-990601 (Clockwise)

Preceding gene: 152974683

Following gene: 152974686

Centisome position: 24.19

GC content: 38.56

Gene sequence:

>1857_bases
ATGATTTATATGTGCGGGATTACTGGATGGATTGACTATAAGCGGGCGTTAGAAGGAGAAAAAGAGACCGTTATGAAGAT
GACGGAAACGCTTGCAAAACGCGGGCCAGATGATACGAATATTTGGATTGATAAACATGTTGCTTTTGGACATAAACGAT
TAATTGTAGTAGATCCAGAAGGCGGAAAACAGCCGATGACTTGTCTAAAGGAAGAATCCAACTATACAATTTGTTACAAT
GGCGAGCTATATAATACAGAAGATATTCGAAAAGAGTTATTGCGAAGAGGTTACACATTTAAAGGACATTCCGATACTGA
GGTGCTATTAGCTTCTTATATAGAATGGAAAGAAGAGTGTGTTAATCATTTAAACGGTATATATGCTTTTGCAGTATGGG
ACGAGAAAAAAGAGCATATTTTTATCGCAAGAGATCGTTTAGGGGTAAAACCATTATTTTATAAATACGATAGCGGAAGA
TTACTGTTCGGTTCAGAACTGAAAGTTATATTGGTGCATCCTGATGTCAAGGCGGAAGTAACGATAGAAGGTTTGGCAGA
AATATTTGGACTTGGCCCATCTAGAACTCCTGGACATGGTGTATATGCTGGTATTCAAGAATTGCGGCCAGGCCACGCAC
TTACATTTTCAAAAGATGGTTTATGTGTATGGAGATATTGGAATGTAGAAAGTAAGAGGCATGAAGATTCATTTGAAGAA
ACGGTCGAGAAAACAAGGTTTTTATTACAAGATGCAATTACGAGACAATTGGTTTCAGATGTACCGCTATGCACATTTTT
GTCGGGCGGAGTCGATTCAAGTGCAATAACGGCAATTGCCGCAAAAGAATATGAGCGCTCCGGAAAGGGACAGTTACACA
CATATTCTATCGATTATGAAGATAATGAAAAATATTTTCAAGCAAACGCTTTTCAGCCAAATTCAGATGCACCATTCATT
GACCTCATGACGGAAACGTTCCGTACAATCCATCACCGCTGCGTCATTTCTAATGAAAGGTTAGCAGAATATTTAACAGA
GGCTGTACTTGTTCGTGATTTACCAGGTATGGCAGATATTGATTCATCTTTGTTATGGTTTTGCCGAGAAATAAAACAAA
AGTTTGTCGTGGGTTTATCGGGGGAGTGTGCAGATGAGATATTTGGTGGTTATCCATGGTTTTATAGGGAAGATGATTTA
CAATCTAGTGCATTTCCGTGGATGAGATCAACGAGGGCGCGGGAGCAACTTTTAAAGGAGGAATGGCGCCGAAAATTAAG
GCTGCAAGAGTATGTGCAGCAACGTTATGAGGAGTCGGTACAAGAAGTACCAGTTTTAGAAGGTGAAAGCACAGTGGAAG
CGAAACGACGACAATTATTCTATTTCAATATGATTTGGTTTATGACAACATTATTAGACAGGAAAGATCGCATGAGTATG
GGAGCAAGCTTAGAAGTGCGCGTACCATTTGCAGATCATCGCCTTGTCGAATATGCATGGAATATTCCTTGGGAAATGAA
AATGTATAAAAATCGCGAAAAAGGTCTACTGCGAAAAGCGTTAGAAGGTATATTACCCGATGATATTTTATATAGAAAGA
AAAGCCCATACCCAAAGACGCATAATCCTCATTATACGAAAGTAGTAACAAGATGGTTACAAGAGTTATTAGAGGATAAG
GGATCGATTTTACATGAATTATTTGATAGAAAAAAATTACAAGAATTAATTGAATCAGGGGGGAGTGCGTTTCAAACACC
GTGGTTCGGACAATTAATGACCGGACCACAACTTTTGGCACATGTAGCACAGATTCATATTTGGTTTAAAGAATACGGAG
TAAATATTAAAGAATAG

Upstream 100 bases:

>100_bases
AGAACCAATTAGGCTTTTACATGTCATATCTTCATTTTTCCTTTTTGGTTCTATTCAGCTTTTGAACGGAGTAAAGTTAT
ACGGATATAGGATAGGAAGG

Downstream 100 bases:

>100_bases
GAAAAAATGGCTCACGCGTGTTGTGAGCCATTTTTATTTTCACCAATCGGGGTGTTAATGCAGGAGAAAATATCAAATAA
AATCCATAATCATATTATAA

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MIYMCGITGWIDYKRALEGEKETVMKMTETLAKRGPDDTNIWIDKHVAFGHKRLIVVDPEGGKQPMTCLKEESNYTICYN
GELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVNHLNGIYAFAVWDEKKEHIFIARDRLGVKPLFYKYDSGR
LLFGSELKVILVHPDVKAEVTIEGLAEIFGLGPSRTPGHGVYAGIQELRPGHALTFSKDGLCVWRYWNVESKRHEDSFEE
TVEKTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNEKYFQANAFQPNSDAPFI
DLMTETFRTIHHRCVISNERLAEYLTEAVLVRDLPGMADIDSSLLWFCREIKQKFVVGLSGECADEIFGGYPWFYREDDL
QSSAFPWMRSTRAREQLLKEEWRRKLRLQEYVQQRYEESVQEVPVLEGESTVEAKRRQLFYFNMIWFMTTLLDRKDRMSM
GASLEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGILPDDILYRKKSPYPKTHNPHYTKVVTRWLQELLEDK
GSILHELFDRKKLQELIESGGSAFQTPWFGQLMTGPQLLAHVAQIHIWFKEYGVNIKE

Sequences:

>Translated_618_residues
MIYMCGITGWIDYKRALEGEKETVMKMTETLAKRGPDDTNIWIDKHVAFGHKRLIVVDPEGGKQPMTCLKEESNYTICYN
GELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVNHLNGIYAFAVWDEKKEHIFIARDRLGVKPLFYKYDSGR
LLFGSELKVILVHPDVKAEVTIEGLAEIFGLGPSRTPGHGVYAGIQELRPGHALTFSKDGLCVWRYWNVESKRHEDSFEE
TVEKTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNEKYFQANAFQPNSDAPFI
DLMTETFRTIHHRCVISNERLAEYLTEAVLVRDLPGMADIDSSLLWFCREIKQKFVVGLSGECADEIFGGYPWFYREDDL
QSSAFPWMRSTRAREQLLKEEWRRKLRLQEYVQQRYEESVQEVPVLEGESTVEAKRRQLFYFNMIWFMTTLLDRKDRMSM
GASLEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGILPDDILYRKKSPYPKTHNPHYTKVVTRWLQELLEDK
GSILHELFDRKKLQELIESGGSAFQTPWFGQLMTGPQLLAHVAQIHIWFKEYGVNIKE
>Mature_618_residues
MIYMCGITGWIDYKRALEGEKETVMKMTETLAKRGPDDTNIWIDKHVAFGHKRLIVVDPEGGKQPMTCLKEESNYTICYN
GELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVNHLNGIYAFAVWDEKKEHIFIARDRLGVKPLFYKYDSGR
LLFGSELKVILVHPDVKAEVTIEGLAEIFGLGPSRTPGHGVYAGIQELRPGHALTFSKDGLCVWRYWNVESKRHEDSFEE
TVEKTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYEDNEKYFQANAFQPNSDAPFI
DLMTETFRTIHHRCVISNERLAEYLTEAVLVRDLPGMADIDSSLLWFCREIKQKFVVGLSGECADEIFGGYPWFYREDDL
QSSAFPWMRSTRAREQLLKEEWRRKLRLQEYVQQRYEESVQEVPVLEGESTVEAKRRQLFYFNMIWFMTTLLDRKDRMSM
GASLEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGILPDDILYRKKSPYPKTHNPHYTKVVTRWLQELLEDK
GSILHELFDRKKLQELIESGGSAFQTPWFGQLMTGPQLLAHVAQIHIWFKEYGVNIKE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=408, Percent_Identity=27.2058823529412, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI168229252, Length=408, Percent_Identity=27.2058823529412, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI168229250, Length=408, Percent_Identity=27.2058823529412, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI296010848, Length=381, Percent_Identity=27.2965879265092, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI296010852, Length=311, Percent_Identity=25.4019292604502, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI296010850, Length=311, Percent_Identity=25.4019292604502, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786889, Length=557, Percent_Identity=25.8527827648115, Blast_Score=156, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=581, Percent_Identity=26.3339070567986, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71993933, Length=581, Percent_Identity=26.3339070567986, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560178, Length=547, Percent_Identity=26.8738574040219, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=310, Percent_Identity=27.741935483871, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=569, Percent_Identity=24.780316344464, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325403, Length=572, Percent_Identity=24.8251748251748, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45553209, Length=525, Percent_Identity=26.2857142857143, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72030; Mature: 72030

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYMCGITGWIDYKRALEGEKETVMKMTETLAKRGPDDTNIWIDKHVAFGHKRLIVVDPE
CEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCC
GGKQPMTCLKEESNYTICYNGELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEEC
CCCCCHHHHHCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
VNHLNGIYAFAVWDEKKEHIFIARDRLGVKPLFYKYDSGRLLFGSELKVILVHPDVKAEV
HHHCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCEEECCCEEEEEECCCCCEEE
TIEGLAEIFGLGPSRTPGHGVYAGIQELRPGHALTFSKDGLCVWRYWNVESKRHEDSFEE
EHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCHHHHH
TVEKTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYE
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCC
DNEKYFQANAFQPNSDAPFIDLMTETFRTIHHRCVISNERLAEYLTEAVLVRDLPGMADI
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCC
DSSLLWFCREIKQKFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTRAREQLLKE
HHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
EWRRKLRLQEYVQQRYEESVQEVPVLEGESTVEAKRRQLFYFNMIWFMTTLLDRKDRMSM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
GASLEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGILPDDILYRKKSPYPKT
CCEEEEECCCCCCHHHHHHHCCCEEHHHHHCHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
HNPHYTKVVTRWLQELLEDKGSILHELFDRKKLQELIESGGSAFQTPWFGQLMTGPQLLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHH
HVAQIHIWFKEYGVNIKE
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIYMCGITGWIDYKRALEGEKETVMKMTETLAKRGPDDTNIWIDKHVAFGHKRLIVVDPE
CEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCC
GGKQPMTCLKEESNYTICYNGELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEEC
CCCCCHHHHHCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
VNHLNGIYAFAVWDEKKEHIFIARDRLGVKPLFYKYDSGRLLFGSELKVILVHPDVKAEV
HHHCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCEEECCCEEEEEECCCCCEEE
TIEGLAEIFGLGPSRTPGHGVYAGIQELRPGHALTFSKDGLCVWRYWNVESKRHEDSFEE
EHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCHHHHH
TVEKTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERSGKGQLHTYSIDYE
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCC
DNEKYFQANAFQPNSDAPFIDLMTETFRTIHHRCVISNERLAEYLTEAVLVRDLPGMADI
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCC
DSSLLWFCREIKQKFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTRAREQLLKE
HHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
EWRRKLRLQEYVQQRYEESVQEVPVLEGESTVEAKRRQLFYFNMIWFMTTLLDRKDRMSM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
GASLEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGILPDDILYRKKSPYPKT
CCEEEEECCCCCCHHHHHHHCCCEEHHHHHCHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
HNPHYTKVVTRWLQELLEDKGSILHELFDRKKLQELIESGGSAFQTPWFGQLMTGPQLLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHH
HVAQIHIWFKEYGVNIKE
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]