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The map label for this gene is mls [H]

Identifier: 152974666

GI number: 152974666

Start: 969280

End: 970869

Strand: Direct

Name: mls [H]

Synonym: Bcer98_0853

Alternate gene names: 152974666

Gene position: 969280-970869 (Clockwise)

Preceding gene: 152974665

Following gene: 152974667

Centisome position: 23.72

GC content: 40.25

Gene sequence:

>1590_bases
ATGTCAACGCAAACTTCACGAGTGACACTCGCTGGAGAATTATTACCATCGTACAACGAAATTTTGACACCAGAAGCGCT
TACTTTTTTGAAAGAGTTGCATGAGAATTTCAATGAACGTCGTATTCAGTTATTAAAGAAACGAGAAGAAAAACAGAAAA
GAATTGATGCGGGAGAGTTTCCGAATTTTTTACCAGAAACAGCACATATACGTGCGGGCAATTGGACAATTGCGCCGCTT
CCGCGTGATTTAGAAGATCGACGCGTAGAGATTACGGGACCCGTAGATCGAAAGATGATTATTAATGCTTTAAACTCTGG
GGCACATCTTTTTATGGCAGATTTTGAAGACTCGAGTTCACCAACATGGAAAAACATAATAGAAGGTCAAATAAACTTAA
GAGATGCTGCGAAAGGAACGATTTCATATAAGAGCCCAAGTGGGAAAGAATACCGTCTTGGCGATCAAACAGCTGTACTT
ATTGTACGTCCGCGCGGTTGGCATTTAGAAGAAAAGCATATGCAAGTTGACGGGAAACGTATGTCAGGAAGTCTTGTAGA
CTTTGGACTGTACTTTTTCCATAATGCGAAAACTTTAATTGAAAAAGGAAGCGGTCCATATTTTTACTTGCCAAAGATGG
AGAGCTATTTAGAGGCTCGTTTATGGAATGATGTATTTGTATTTGCACAGAAGTATGTCGGTATTCCAAATGGCACAATT
AAAGCGACGGTACTGCTTGAGACGATCCATGCTGCATTTGAAATGGATGAAATTTTATATGAATTACGTGATCACTCCGC
TGGATTAAATTGTGGACGATGGGATTATATCTTTAGCTTTTTAAAATGTTTCCGCAATCATAAAGCATTTTTGCTTCCAG
ACAGAGCACAAGTTACGATGACAGTACCGTTTATGCGCGCGTATTCTTTGAAAGTCATTCAAACGTGTCACCGCCGAAAT
GCACCAGCGATGGGGGGGATGGCAGCGCAAATTCCAATTAAAAATAATCCAGAGGCGAATGAAATCGCCTTTGAAAAAGT
GCGGGTCGATAAAGAGCGCGAAGCTTTAGATGGGCATGATGGAACTTGGGTTGCTCATCCAGGGCTCGTACCAGTTGCGA
TGGAAGTATTTAATCACATTATGAAAACACCAAATCAAATATTTAGAAAACGGGAGGAACTGCATGTAACAGAAAAAGAT
TTATTAGAAGTACCTATGGGAACGATTACAGAAGAAGGGCTGCGCATGAATATCAGTGTTGGCATTCAATATATTGCGTC
ATGGCTAAGTGGACGCGGGGCAGCACCTATTTATAACTTAATGGAAGATGCGGCAACAGCGGAAATATCAAGAGCGCAAG
TTTGGCAGTGGATTCGTCATGAGGAGGGGAAACTGAATGACGGTCGTAAAGTTACGTTTGCTTTAGTAGAACAATTGAAG
GCGGAAGAACTAGCGAAAATAGAGAAGGAGATTGGAAAAGAATCATTTGAGAAAGGTAGATTTGTAGAAGCGACAAAGTT
ATTTACTGATTTAGTGCGTAAGGATGAGTTTGAACCATTTTTAACATTACCTGGTTATGAAATTTTATAA

Upstream 100 bases:

>100_bases
GATTTTTCAAAAAACACCTTCCTTTTCTGAAATTTTGTTATATAATATAAAGGGTAAATATAAAACTGTTATAAGACAGT
TTAAAAAAGGAGATGCTAAT

Downstream 100 bases:

>100_bases
AAGAAAAGGGGATGGAAGAAATGAAAAATGAAAGAGTTCAGCAATTGCAAGAGGATTGGGAATTAAATGCACGCTGGAAG
GGGATTACGCGTCCTTATTC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSTQTSRVTLAGELLPSYNEILTPEALTFLKELHENFNERRIQLLKKREEKQKRIDAGEFPNFLPETAHIRAGNWTIAPL
PRDLEDRRVEITGPVDRKMIINALNSGAHLFMADFEDSSSPTWKNIIEGQINLRDAAKGTISYKSPSGKEYRLGDQTAVL
IVRPRGWHLEEKHMQVDGKRMSGSLVDFGLYFFHNAKTLIEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYVGIPNGTI
KATVLLETIHAAFEMDEILYELRDHSAGLNCGRWDYIFSFLKCFRNHKAFLLPDRAQVTMTVPFMRAYSLKVIQTCHRRN
APAMGGMAAQIPIKNNPEANEIAFEKVRVDKEREALDGHDGTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREELHVTEKD
LLEVPMGTITEEGLRMNISVGIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEEGKLNDGRKVTFALVEQLK
AEELAKIEKEIGKESFEKGRFVEATKLFTDLVRKDEFEPFLTLPGYEIL

Sequences:

>Translated_529_residues
MSTQTSRVTLAGELLPSYNEILTPEALTFLKELHENFNERRIQLLKKREEKQKRIDAGEFPNFLPETAHIRAGNWTIAPL
PRDLEDRRVEITGPVDRKMIINALNSGAHLFMADFEDSSSPTWKNIIEGQINLRDAAKGTISYKSPSGKEYRLGDQTAVL
IVRPRGWHLEEKHMQVDGKRMSGSLVDFGLYFFHNAKTLIEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYVGIPNGTI
KATVLLETIHAAFEMDEILYELRDHSAGLNCGRWDYIFSFLKCFRNHKAFLLPDRAQVTMTVPFMRAYSLKVIQTCHRRN
APAMGGMAAQIPIKNNPEANEIAFEKVRVDKEREALDGHDGTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREELHVTEKD
LLEVPMGTITEEGLRMNISVGIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEEGKLNDGRKVTFALVEQLK
AEELAKIEKEIGKESFEKGRFVEATKLFTDLVRKDEFEPFLTLPGYEIL
>Mature_528_residues
STQTSRVTLAGELLPSYNEILTPEALTFLKELHENFNERRIQLLKKREEKQKRIDAGEFPNFLPETAHIRAGNWTIAPLP
RDLEDRRVEITGPVDRKMIINALNSGAHLFMADFEDSSSPTWKNIIEGQINLRDAAKGTISYKSPSGKEYRLGDQTAVLI
VRPRGWHLEEKHMQVDGKRMSGSLVDFGLYFFHNAKTLIEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYVGIPNGTIK
ATVLLETIHAAFEMDEILYELRDHSAGLNCGRWDYIFSFLKCFRNHKAFLLPDRAQVTMTVPFMRAYSLKVIQTCHRRNA
PAMGGMAAQIPIKNNPEANEIAFEKVRVDKEREALDGHDGTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREELHVTEKDL
LEVPMGTITEEGLRMNISVGIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEEGKLNDGRKVTFALVEQLKA
EELAKIEKEIGKESFEKGRFVEATKLFTDLVRKDEFEPFLTLPGYEIL

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=512, Percent_Identity=54.4921875, Blast_Score=564, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17561814, Length=525, Percent_Identity=55.047619047619, Blast_Score=585, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71982926, Length=418, Percent_Identity=57.6555023923445, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6324212, Length=515, Percent_Identity=53.2038834951456, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6322222, Length=515, Percent_Identity=51.8446601941748, Blast_Score=528, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60487; Mature: 60356

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQTSRVTLAGELLPSYNEILTPEALTFLKELHENFNERRIQLLKKREEKQKRIDAGEF
CCCCCCEEEEEHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PNFLPETAHIRAGNWTIAPLPRDLEDRRVEITGPVDRKMIINALNSGAHLFMADFEDSSS
CCCCCCCCEEECCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCC
PTWKNIIEGQINLRDAAKGTISYKSPSGKEYRLGDQTAVLIVRPRGWHLEEKHMQVDGKR
CCHHHHHCCCCEEECCCCCEEEECCCCCCCEEECCCEEEEEECCCCCCCCHHHHEECCCC
MSGSLVDFGLYFFHNAKTLIEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYVGIPNGTI
CCCCHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
KATVLLETIHAAFEMDEILYELRDHSAGLNCGRWDYIFSFLKCFRNHKAFLLPDRAQVTM
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEE
TVPFMRAYSLKVIQTCHRRNAPAMGGMAAQIPIKNNPEANEIAFEKVRVDKEREALDGHD
EEHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCC
GTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREELHVTEKDLLEVPMGTITEEGLRMNISV
CCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEHHH
GIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEEGKLNDGRKVTFALVEQLK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AEELAKIEKEIGKESFEKGRFVEATKLFTDLVRKDEFEPFLTLPGYEIL
HHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure 
STQTSRVTLAGELLPSYNEILTPEALTFLKELHENFNERRIQLLKKREEKQKRIDAGEF
CCCCCEEEEEHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PNFLPETAHIRAGNWTIAPLPRDLEDRRVEITGPVDRKMIINALNSGAHLFMADFEDSSS
CCCCCCCCEEECCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCC
PTWKNIIEGQINLRDAAKGTISYKSPSGKEYRLGDQTAVLIVRPRGWHLEEKHMQVDGKR
CCHHHHHCCCCEEECCCCCEEEECCCCCCCEEECCCEEEEEECCCCCCCCHHHHEECCCC
MSGSLVDFGLYFFHNAKTLIEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYVGIPNGTI
CCCCHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
KATVLLETIHAAFEMDEILYELRDHSAGLNCGRWDYIFSFLKCFRNHKAFLLPDRAQVTM
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEE
TVPFMRAYSLKVIQTCHRRNAPAMGGMAAQIPIKNNPEANEIAFEKVRVDKEREALDGHD
EEHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHCCCCC
GTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREELHVTEKDLLEVPMGTITEEGLRMNISV
CCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEHHH
GIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEEGKLNDGRKVTFALVEQLK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AEELAKIEKEIGKESFEKGRFVEATKLFTDLVRKDEFEPFLTLPGYEIL
HHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA