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The map label for this gene is aspA [H]

Identifier: 152974356

GI number: 152974356

Start: 601169

End: 602602

Strand: Direct

Name: aspA [H]

Synonym: Bcer98_0526

Alternate gene names: 152974356

Gene position: 601169-602602 (Clockwise)

Preceding gene: 152974355

Following gene: 152974358

Centisome position: 14.71

GC content: 37.38

Gene sequence:

>1434_bases
ATGATAGCGACAAAAGACATACGGATAGAAAAAGATTTCTTAGGTGAGAAAGAAGTACCAAATGAAGCTTATTATGGAGT
CCAAACTTTACGTGCTGTGGAAAACTTTCCAATTACAGGATATCGTATACATGATTCACTCATCACTGCAATGGCGATTG
TAAAGAAAGCGGCAGCGCTTGCCAATGTGGAAACAGGTTATTTACCAGAGAACATTGGTAAGGAAATTGCAGCAGCAGCT
CAGGAAATTATGGATGGTAAACTTCATGATCAATTTATTGTCGATCCTATTCAAGGTGGGGCTGGAACTTCCATTAACAT
GAATGCGAATGAAGTAATTGCAAATCGTGCATTAGAACGGATGGGATATGAAAAAGGGGATTATGCAAAAATTAGTCCGA
ATACACATGTCAATATGGCACAATCCACAAATGATGCATTTCCAACAGGAATTCATATTGCAACTTTAATGATGTTAGAG
GAGCTTCTTATTACGATGGAAGAGCTTCATCGTGCATTTATAAAGAAAGCGAAAGAATTTGATCATGTCATTAAAATGGG
ACGTACGCACTTACAAGATGCTGTTCCTATTCGACTTGGACAAGAATTTAGCGCATATAGTCGTGTATTAGAACGTGATA
TAAAAAGAATTAAACAATCTCGTCAGCATTTATATGAAGTTAATATGGGCGCAACAGCTGTTGGCACAGGATTAAATGCA
AATCCAATGTATATTGAACAAGTTGTAAAGCATTTACGAGAATTAAGTGGTTTTCCACTTGTAGGAGCGGAGCATTTAGT
AGATGCAACGCAAAATACGGATGCATATACAGAAGTATCCGCAGCGCTTAAAGTATGTATGATGAATATGTCTAAGATTG
CAAATGACTTACGTATTATGGCTTCTGGTCCAAGAGTTGGATTAGCAGAGATTCAATTGCCAGCTCGTCAGCCAGGTTCA
TCTATTATGCCTGGGAAAGTTAATCCGGTTATGGCTGAAGTGATTAATCAAGTTGCCTTCCAAGTGATTGGGAACGACCA
TACGATTTGTTTAGCATCTGAAGCAGGGCAATTAGAATTAAATGTAATGGAGCCAGTTCTCGTATTTAATTTAATCCAAT
CTATTAGTATTATGAATAATGCATTTCGTGTATTCCGTGAATATTGCATTGAAGGTATTACGGCGAATGAAGAACTATTG
AAAGAATATGTGGAACGAAGTGTTGGGATTATTACAGCAGTCAATCCTCATATTGGATATGAAGCTGCTTCTCGCATTGC
TCGTGAAGCAATCGCAACAGGAAAATCAGTTCGAGAACTTTGCCTAGAACATGGTGTTTTAACAGAAGAAGAACTGGATA
TTATTTTGGATCCATATGAAATGACACATCCTGAAATAGCCGGAGCATCATTACTTAAAAATAAAAAATCTTGA

Upstream 100 bases:

>100_bases
TATAGTGGATGGAGATGGAAATGTTTCTATGAAAATGATGTTTTCATAGAAACAATGCTAAAACTTTAAAGATATACAAC
GAAAAGCATAGGTGATAAAT

Downstream 100 bases:

>100_bases
TACGTTCGTATCAAGATTTTTTATTTAGCAAAAGATAATATAACAACGAGACATAAAAGGCTGATGAACGAGTCATCAGC
CTTTTATGTTAAAAAATATG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MIATKDIRIEKDFLGEKEVPNEAYYGVQTLRAVENFPITGYRIHDSLITAMAIVKKAAALANVETGYLPENIGKEIAAAA
QEIMDGKLHDQFIVDPIQGGAGTSINMNANEVIANRALERMGYEKGDYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLE
ELLITMEELHRAFIKKAKEFDHVIKMGRTHLQDAVPIRLGQEFSAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNA
NPMYIEQVVKHLRELSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGPRVGLAEIQLPARQPGS
SIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLIQSISIMNNAFRVFREYCIEGITANEELL
KEYVERSVGIITAVNPHIGYEAASRIAREAIATGKSVRELCLEHGVLTEEELDIILDPYEMTHPEIAGASLLKNKKS

Sequences:

>Translated_477_residues
MIATKDIRIEKDFLGEKEVPNEAYYGVQTLRAVENFPITGYRIHDSLITAMAIVKKAAALANVETGYLPENIGKEIAAAA
QEIMDGKLHDQFIVDPIQGGAGTSINMNANEVIANRALERMGYEKGDYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLE
ELLITMEELHRAFIKKAKEFDHVIKMGRTHLQDAVPIRLGQEFSAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNA
NPMYIEQVVKHLRELSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGPRVGLAEIQLPARQPGS
SIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLIQSISIMNNAFRVFREYCIEGITANEELL
KEYVERSVGIITAVNPHIGYEAASRIAREAIATGKSVRELCLEHGVLTEEELDIILDPYEMTHPEIAGASLLKNKKS
>Mature_477_residues
MIATKDIRIEKDFLGEKEVPNEAYYGVQTLRAVENFPITGYRIHDSLITAMAIVKKAAALANVETGYLPENIGKEIAAAA
QEIMDGKLHDQFIVDPIQGGAGTSINMNANEVIANRALERMGYEKGDYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLE
ELLITMEELHRAFIKKAKEFDHVIKMGRTHLQDAVPIRLGQEFSAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNA
NPMYIEQVVKHLRELSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGPRVGLAEIQLPARQPGS
SIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLIQSISIMNNAFRVFREYCIEGITANEELL
KEYVERSVGIITAVNPHIGYEAASRIAREAIATGKSVRELCLEHGVLTEEELDIILDPYEMTHPEIAGASLLKNKKS

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=468, Percent_Identity=43.3760683760684, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI68303547, Length=211, Percent_Identity=27.4881516587678, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI31541964, Length=209, Percent_Identity=27.7511961722488, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI68303542, Length=209, Percent_Identity=27.7511961722488, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI87082375, Length=464, Percent_Identity=52.5862068965517, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=40.6047516198704, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1790398, Length=209, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=44.1558441558442, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=42.3780487804878, Blast_Score=281, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=44.2764578833693, Blast_Score=393, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24640177, Length=468, Percent_Identity=41.6666666666667, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640179, Length=468, Percent_Identity=41.6666666666667, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=42.2413793103448, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24662684, Length=471, Percent_Identity=41.1889596602972, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24583245, Length=468, Percent_Identity=37.3931623931624, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52624; Mature: 52624

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATKDIRIEKDFLGEKEVPNEAYYGVQTLRAVENFPITGYRIHDSLITAMAIVKKAAAL
CCCCCCCEECHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
ANVETGYLPENIGKEIAAAAQEIMDGKLHDQFIVDPIQGGAGTSINMNANEVIANRALER
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEECCCHHHHHHHHHHHH
MGYEKGDYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLEELLITMEELHRAFIKKAKEF
CCCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHVIKMGRTHLQDAVPIRLGQEFSAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNA
HHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
NPMYIEQVVKHLRELSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIM
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
ASGPRVGLAEIQLPARQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLEL
ECCCCCCHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVLVFNLIQSISIMNNAFRVFREYCIEGITANEELLKEYVERSVGIITAVNPHIGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCCH
EAASRIAREAIATGKSVRELCLEHGVLTEEELDIILDPYEMTHPEIAGASLLKNKKS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIATKDIRIEKDFLGEKEVPNEAYYGVQTLRAVENFPITGYRIHDSLITAMAIVKKAAAL
CCCCCCCEECHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHH
ANVETGYLPENIGKEIAAAAQEIMDGKLHDQFIVDPIQGGAGTSINMNANEVIANRALER
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEECCCHHHHHHHHHHHH
MGYEKGDYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLEELLITMEELHRAFIKKAKEF
CCCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHVIKMGRTHLQDAVPIRLGQEFSAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNA
HHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
NPMYIEQVVKHLRELSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIM
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
ASGPRVGLAEIQLPARQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLEL
ECCCCCCHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVLVFNLIQSISIMNNAFRVFREYCIEGITANEELLKEYVERSVGIITAVNPHIGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCCH
EAASRIAREAIATGKSVRELCLEHGVLTEEELDIILDPYEMTHPEIAGASLLKNKKS
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]