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The map label for this gene is mrcA [H]

Identifier: 152974299

GI number: 152974299

Start: 527437

End: 528258

Strand: Direct

Name: mrcA [H]

Synonym: Bcer98_0466

Alternate gene names: 152974299

Gene position: 527437-528258 (Clockwise)

Preceding gene: 152974298

Following gene: 152974300

Centisome position: 12.91

GC content: 32.36

Gene sequence:

>822_bases
ATGAAAGAGCGTGTATTATCTAGAGTGAATTATCATCAAAAAATTTCATTCAATCCGATTACAAAGTTTCTTTATAAAGC
TATTATTTTATTATTATTACTAAGTTGTACTTTATTATTTATCGGAAATGTAATGATTGAACGTAGTGATATTAGCAAGC
TGCAAGTTCCTGCTAAATTAGAGATACCAAGCTCGTTGGCTCACGCATTTATTGCGACTGAAGATAAAAGGTTTTACCAT
CATAATGGATTAGATTATATTGCAATTGTTCGTGCGTCTATTGAAAATATAAAAGCGGGTGGTATTGTACAAGGAGGAAG
TACAATTACACAGCAGTTATCTAAAAATGCTTTTTTATCTAATGAGCGCACATTTTCTCGAAAATGGAAAGAAATTTTTT
ATACAAAAAAAATTGAGCGTACATTTACGAAAGATGAAATTTTGAAATTGTATGTGAGTAATATTTATTATGGAGAAGGT
GCATGGGGAATTGAAAAAGCAGCAAAGTTATATTTTGGTAAAAAGGTTAACAAACTAACATTAAGTGAAAGTGCAATGTT
AGCTGCTGTTGTAAAGGCACCTGCGTATTATTCACCTGCAAAAAATTACAATAAAGCTGTGGAACGAAGAAACGTAGTCT
TAAAGCTAATGGAGCGAGAGGGATATATTAATCATAACCAATATGTGCAAGCACTAAGTGAGAAACTTGTTATTCGTCAC
GATCCAAAAACAGAGCATTTGATACAAAAGACAGCACGTGAAAAGCTTGTTAGTGAAAGGGAGTTCCGAGAGAGGAGCTT
CTTTTTTATGGATAAAATATGA

Upstream 100 bases:

>100_bases
TTGTAAGATAGGTGTAAGAGAAATCGATTGGATCTTCTTTTGGAAGACGATATTCTTAAATTAAGAATAATATATTCTAT
ATAGAAAAGAGGTGGGCACG

Downstream 100 bases:

>100_bases
ACAGAAGAGACACAATATTGTCACATTTACTCTATTTTTGGAATTTATGTGAGCGTGTTCATTGCATGACTGATATAAGT
ATTGATATTATGTGTATTGT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKERVLSRVNYHQKISFNPITKFLYKAIILLLLLSCTLLFIGNVMIERSDISKLQVPAKLEIPSSLAHAFIATEDKRFYH
HNGLDYIAIVRASIENIKAGGIVQGGSTITQQLSKNAFLSNERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEG
AWGIEKAAKLYFGKKVNKLTLSESAMLAAVVKAPAYYSPAKNYNKAVERRNVVLKLMEREGYINHNQYVQALSEKLVIRH
DPKTEHLIQKTAREKLVSEREFRERSFFFMDKI

Sequences:

>Translated_273_residues
MKERVLSRVNYHQKISFNPITKFLYKAIILLLLLSCTLLFIGNVMIERSDISKLQVPAKLEIPSSLAHAFIATEDKRFYH
HNGLDYIAIVRASIENIKAGGIVQGGSTITQQLSKNAFLSNERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEG
AWGIEKAAKLYFGKKVNKLTLSESAMLAAVVKAPAYYSPAKNYNKAVERRNVVLKLMEREGYINHNQYVQALSEKLVIRH
DPKTEHLIQKTAREKLVSEREFRERSFFFMDKI
>Mature_273_residues
MKERVLSRVNYHQKISFNPITKFLYKAIILLLLLSCTLLFIGNVMIERSDISKLQVPAKLEIPSSLAHAFIATEDKRFYH
HNGLDYIAIVRASIENIKAGGIVQGGSTITQQLSKNAFLSNERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEG
AWGIEKAAKLYFGKKVNKLTLSESAMLAAVVKAPAYYSPAKNYNKAVERRNVVLKLMEREGYINHNQYVQALSEKLVIRH
DPKTEHLIQKTAREKLVSEREFRERSFFFMDKI

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=185, Percent_Identity=49.1891891891892, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786343, Length=163, Percent_Identity=40.4907975460123, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1789601, Length=229, Percent_Identity=31.4410480349345, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788867, Length=185, Percent_Identity=30.2702702702703, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 31623; Mature: 31623

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERVLSRVNYHQKISFNPITKFLYKAIILLLLLSCTLLFIGNVMIERSDISKLQVPAKL
CCHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCCEE
EIPSSLAHAFIATEDKRFYHHNGLDYIAIVRASIENIKAGGIVQGGSTITQQLSKNAFLS
CCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCC
NERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEGAWGIEKAAKLYFGKKVNKLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCEEE
LSESAMLAAVVKAPAYYSPAKNYNKAVERRNVVLKLMEREGYINHNQYVQALSEKLVIRH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEC
DPKTEHLIQKTAREKLVSEREFRERSFFFMDKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKERVLSRVNYHQKISFNPITKFLYKAIILLLLLSCTLLFIGNVMIERSDISKLQVPAKL
CCHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHCCCCEE
EIPSSLAHAFIATEDKRFYHHNGLDYIAIVRASIENIKAGGIVQGGSTITQQLSKNAFLS
CCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCC
NERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEGAWGIEKAAKLYFGKKVNKLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCEEE
LSESAMLAAVVKAPAYYSPAKNYNKAVERRNVVLKLMEREGYINHNQYVQALSEKLVIRH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEC
DPKTEHLIQKTAREKLVSEREFRERSFFFMDKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3882429; 9278503; 3920658; 7006606; 9841666 [H]