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The map label for this gene is pflB [H]

Identifier: 152974264

GI number: 152974264

Start: 486574

End: 488823

Strand: Direct

Name: pflB [H]

Synonym: Bcer98_0431

Alternate gene names: 152974264

Gene position: 486574-488823 (Clockwise)

Preceding gene: 152974263

Following gene: 152974265

Centisome position: 11.91

GC content: 36.84

Gene sequence:

>2250_bases
ATGACTCAAGTATTAGAGACTGTAAAAAATGCATGGGAAAAATTTAAAGGTGAAAAATGGAAAGTTGAGATTGATGTTCG
CGATTTCATTCTAAATAATGTAAACGTTTACAACGGGGACGAATCCTTCTTAGCAGGAGCAACAGAAGCAACGAAAAAAC
TTTGGGATCAAGTAATGGATCTAACTAAACAAGAACGTGAAAATGGTGGCGTTCTTGATATGGACACAAAAATTGTTTCT
TCTATTACATCACATGGTCCAGGGTATTTAAATAAGGAGCTTGAAAAAGTAGTAGGTGTTCAAACAGATCAGCCATTTAA
ACGTTCTTTACAACCATTTGGTGGTATTCGTATGGCAGAGCAATCTTGTGAAGCATATGGATTTAAAGTGGATGAGGAAT
TAAGTCGTATCTTTAGAGATTGGCGCAAAACTCATAACCAAGGTGTATTTGATGCTTACACTCCAGAAATGAGAGCAGCT
CGTAAATCAGGTGTTATTACTGGTCTTCCAGACGCATATGGACGCGGACGTATTATTGGTGATTATCGCCGCGTAGCACT
ATATGGGGTAGATCGTTTAATTGAAGCGAAAGAAGAGGATTTAAACCTAACAGGAAGTGTAATGAGTGAAGAGGTTATCC
GTTTACGTGAAGAAGTATCTGAACAAATTCGTGCACTTCAAGAATTAAAAGAAATGGCAGCTTCTCACGGTTTTGATATT
TCAAAACCAGCGACAAATGCACGAGAAGCATTCCAATGGTTATACTTTGCTTATCTTGCAGCAATTAAAGAACAAAACGG
GGCTGCAATGAGTCTTGGACGTACTTCAACATTCTTAGATATTTATGTTGAAAGAGATTTAGAAAATGGCACTTTAACTG
AAGAGGAAGCCCAAGAGATTGTTGACCATTTCATTATGAAGTTACGTCTTGTAAAATTTGCAAGAACTCCTGATTATAAT
GAATTATTCTCTGGTGATCCAACTTGGGTAACTGAATCTATCGGTGGTATTGCACTTGACGGTCGTCCATTAGTAACAAA
AAACTCATTCCGTTTCTTGCATACATTGGACAATTTAGGACCTGCGCCAGAGCCAAACTTAACAGTTCTTTGGTCTAAGC
AATTACCACAGAAATTTAAAAACTACTGTGCAAAAATGTCTATTAAAACATCTGCAATCCAATATGAAAATGACGACATT
ATGCGTCCAGAATATGGCGATGACTATGGTATTGCTTGCTGTGTATCCGCAATGAAAATTGGTAAACAAATGCAATTCTT
CGGAGCTCGTGCAAACTTAGCGAAGGCTTTATTATACGCTATTAATGGTGGGAAAGACGAAAAATCAAAAGTGCAAGTAG
GGCCAGAATTTGCACCAATTACTTCCGACGTACTAGATTATGATGAAGTTATGCATAAATTTGATATGACAATGGAATGG
TTAGCGGGTCTTTACTTAAATACATTAAATGTAATTCATTACATGCACGACAAATATAGCTATGAACGTATCGAAATGGC
ACTTCATGATACAGAAGTTCTTCGCACAATGGCAACAGGGATCGCTGGTCTATCTGTCGTAGCTGACTCTTTAAGTGCAA
TTAAATATGCAAAAGTAAAACCAATTCGCGATGAAAATGGTATCGCGGTTGACTTTGAAATTGAAGGTGACTTCCCGAAA
TATGGTAACAATGATGACCGCGTTGATGAAATTGCAGTAAATCTTGTGAAAACATTTATGAACAAACTTCGTAAACATAA
AACATACCGTAATTCTGTTCATACAATGTCAATCTTAACAATTACATCTAATGTGGTATATGGTAAGAAAACTGGTAATA
CTCCAGATGGACGCCGTGCTGGAGAACCATTCGCACCAGGGGCAAATCCAATGCATGGACGTGATACAAAAGGTGCACTA
GCATCGCTATTATCTGTTGCGAAATTACCATACGAAGATGCGCAAGATGGTATTTCTAATACATTCTCCATCATTCCGAA
AGCGCTTGGTAAAGAAGAAGAGGTACAAGTTAGTAACTTAGTATCTATGTTAGATGGATATGCAGTAAAAGAAGGACATC
ACTTAAATATTAACGTATTTAATCGTGAAACATTATTGGATGCAATGGAACATCCTGAAAAATATCCACAATTAACAATT
CGTGTATCTGGTTATGCTGTTAACTTTATTAAATTAACTCGTGAACAACAAATTGATGTAATTAATCGTACAATGCATGA
AAGCATGTAA

Upstream 100 bases:

>100_bases
ACTATCTCGTTCGTTTTTTAAAAAAAGTAGTATTCTAAATGTGTAAGCTGTTATATAAAAAATCTTAGTCCTGTACTAAT
CAAAAATGGAGGAGATTAGG

Downstream 100 bases:

>100_bases
AGAAAAATTTCCCCCTATCTCTTAATAAGGGCACGGGGGAAATTGTTTCAATAAAGGAGGAAGTAACATGGTAAAAGGAA
GAATTCATTCTGTAGAGTCT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MTQVLETVKNAWEKFKGEKWKVEIDVRDFILNNVNVYNGDESFLAGATEATKKLWDQVMDLTKQERENGGVLDMDTKIVS
SITSHGPGYLNKELEKVVGVQTDQPFKRSLQPFGGIRMAEQSCEAYGFKVDEELSRIFRDWRKTHNQGVFDAYTPEMRAA
RKSGVITGLPDAYGRGRIIGDYRRVALYGVDRLIEAKEEDLNLTGSVMSEEVIRLREEVSEQIRALQELKEMAASHGFDI
SKPATNAREAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYVERDLENGTLTEEEAQEIVDHFIMKLRLVKFARTPDYN
ELFSGDPTWVTESIGGIALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQKFKNYCAKMSIKTSAIQYENDDI
MRPEYGDDYGIACCVSAMKIGKQMQFFGARANLAKALLYAINGGKDEKSKVQVGPEFAPITSDVLDYDEVMHKFDMTMEW
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTEVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPK
YGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAL
ASLLSVAKLPYEDAQDGISNTFSIIPKALGKEEEVQVSNLVSMLDGYAVKEGHHLNINVFNRETLLDAMEHPEKYPQLTI
RVSGYAVNFIKLTREQQIDVINRTMHESM

Sequences:

>Translated_749_residues
MTQVLETVKNAWEKFKGEKWKVEIDVRDFILNNVNVYNGDESFLAGATEATKKLWDQVMDLTKQERENGGVLDMDTKIVS
SITSHGPGYLNKELEKVVGVQTDQPFKRSLQPFGGIRMAEQSCEAYGFKVDEELSRIFRDWRKTHNQGVFDAYTPEMRAA
RKSGVITGLPDAYGRGRIIGDYRRVALYGVDRLIEAKEEDLNLTGSVMSEEVIRLREEVSEQIRALQELKEMAASHGFDI
SKPATNAREAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYVERDLENGTLTEEEAQEIVDHFIMKLRLVKFARTPDYN
ELFSGDPTWVTESIGGIALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQKFKNYCAKMSIKTSAIQYENDDI
MRPEYGDDYGIACCVSAMKIGKQMQFFGARANLAKALLYAINGGKDEKSKVQVGPEFAPITSDVLDYDEVMHKFDMTMEW
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTEVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPK
YGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAL
ASLLSVAKLPYEDAQDGISNTFSIIPKALGKEEEVQVSNLVSMLDGYAVKEGHHLNINVFNRETLLDAMEHPEKYPQLTI
RVSGYAVNFIKLTREQQIDVINRTMHESM
>Mature_748_residues
TQVLETVKNAWEKFKGEKWKVEIDVRDFILNNVNVYNGDESFLAGATEATKKLWDQVMDLTKQERENGGVLDMDTKIVSS
ITSHGPGYLNKELEKVVGVQTDQPFKRSLQPFGGIRMAEQSCEAYGFKVDEELSRIFRDWRKTHNQGVFDAYTPEMRAAR
KSGVITGLPDAYGRGRIIGDYRRVALYGVDRLIEAKEEDLNLTGSVMSEEVIRLREEVSEQIRALQELKEMAASHGFDIS
KPATNAREAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYVERDLENGTLTEEEAQEIVDHFIMKLRLVKFARTPDYNE
LFSGDPTWVTESIGGIALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQKFKNYCAKMSIKTSAIQYENDDIM
RPEYGDDYGIACCVSAMKIGKQMQFFGARANLAKALLYAINGGKDEKSKVQVGPEFAPITSDVLDYDEVMHKFDMTMEWL
AGLYLNTLNVIHYMHDKYSYERIEMALHDTEVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPKY
GNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALA
SLLSVAKLPYEDAQDGISNTFSIIPKALGKEEEVQVSNLVSMLDGYAVKEGHHLNINVFNRETLLDAMEHPEKYPQLTIR
VSGYAVNFIKLTREQQIDVINRTMHESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=65.6578947368421, Blast_Score=1060, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=65.3794940079894, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=707, Percent_Identity=27.1570014144272, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI1790388, Length=687, Percent_Identity=24.3085880640466, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=70.4918032786885, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84459; Mature: 84328

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVLETVKNAWEKFKGEKWKVEIDVRDFILNNVNVYNGDESFLAGATEATKKLWDQVMD
CCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCEEECCCHHHHCCCHHHHHHHHHHHHH
LTKQERENGGVLDMDTKIVSSITSHGPGYLNKELEKVVGVQTDQPFKRSLQPFGGIRMAE
HHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHH
QSCEAYGFKVDEELSRIFRDWRKTHNQGVFDAYTPEMRAARKSGVITGLPDAYGRGRIIG
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGVDRLIEAKEEDLNLTGSVMSEEVIRLREEVSEQIRALQELKEMAASHGFDI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKPATNAREAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYVERDLENGTLTEEEAQEI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHH
VDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGIALDGRPLVTKNSFRFLHTLDNLG
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHHHHHCC
PAPEPNLTVLWSKQLPQKFKNYCAKMSIKTSAIQYENDDIMRPEYGDDYGIACCVSAMKI
CCCCCCEEEEECHHHHHHHHHHHHHCCEEHHEEEECCCCCCCCCCCCCCHHHHHHHHHHH
GKQMQFFGARANLAKALLYAINGGKDEKSKVQVGPEFAPITSDVLDYDEVMHKFDMTMEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTEVLRTMATGIAGLSVVADSLSAIKYAKVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILT
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEE
ITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQDGISN
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TFSIIPKALGKEEEVQVSNLVSMLDGYAVKEGHHLNINVFNRETLLDAMEHPEKYPQLTI
HHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCCCEEEE
RVSGYAVNFIKLTREQQIDVINRTMHESM
EEECEEEEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQVLETVKNAWEKFKGEKWKVEIDVRDFILNNVNVYNGDESFLAGATEATKKLWDQVMD
CHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCEEECCCHHHHCCCHHHHHHHHHHHHH
LTKQERENGGVLDMDTKIVSSITSHGPGYLNKELEKVVGVQTDQPFKRSLQPFGGIRMAE
HHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHH
QSCEAYGFKVDEELSRIFRDWRKTHNQGVFDAYTPEMRAARKSGVITGLPDAYGRGRIIG
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGVDRLIEAKEEDLNLTGSVMSEEVIRLREEVSEQIRALQELKEMAASHGFDI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKPATNAREAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYVERDLENGTLTEEEAQEI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHH
VDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGIALDGRPLVTKNSFRFLHTLDNLG
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHHHHHCC
PAPEPNLTVLWSKQLPQKFKNYCAKMSIKTSAIQYENDDIMRPEYGDDYGIACCVSAMKI
CCCCCCEEEEECHHHHHHHHHHHHHCCEEHHEEEECCCCCCCCCCCCCCHHHHHHHHHHH
GKQMQFFGARANLAKALLYAINGGKDEKSKVQVGPEFAPITSDVLDYDEVMHKFDMTMEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTEVLRTMATGIAGLSVVADSLSAIKYAKVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILT
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEE
ITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQDGISN
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TFSIIPKALGKEEEVQVSNLVSMLDGYAVKEGHHLNINVFNRETLLDAMEHPEKYPQLTI
HHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCCCEEEE
RVSGYAVNFIKLTREQQIDVINRTMHESM
EEECEEEEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA