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The map label for this gene is thiM

Identifier: 152974199

GI number: 152974199

Start: 417602

End: 418411

Strand: Direct

Name: thiM

Synonym: Bcer98_0363

Alternate gene names: 152974199

Gene position: 417602-418411 (Clockwise)

Preceding gene: 152974198

Following gene: 152974200

Centisome position: 10.22

GC content: 40.62

Gene sequence:

>810_bases
ATGCATATAACAGAAATTGCTAAAGTGGTGGAACAAGTAAGAAAAACGAATCCACTTGTGCATAATATTACAAATGTAGT
TGTAACGAACTTTACAGCAAATGGCTTGCTAGCGTTAGGGGCATCACCAGTCATGGCATATGCAAAAGAAGAAGTAGCAG
AGATGGCTAGCATTGCAGGAGCACTCGTTTTAAATATGGGTACGCTCCGGCCAGATGAAGTTGAAGCAATGCTCATTGCT
GGAAAAGCAGCGAATCAACAGCATGTCCCAGTATTATTTGATCCAGTTGGGGCAGGAGCAACTTTGTATCGAACAGAAGT
GGCAAGACATATACCAAATGAAATAAAGTTAGCGATGATCCGCGGAAATGCAGCGGAAATTGCGAATGTTATTCATGAAA
AATGGGAAATCAAAGGTGTGGATGCTGGAACTGGAAATGGTGATTCTGTAGCAATTGCAACAAAAGCAGCGAACAAACTA
GGTACAGTCGCGGTAATTACAGGAAAAGAAGATATTGTCACAGATGGTAAGCGAACCGTTATAATTCGTAACGGTCATCC
AATTTTAACAAAGGTGACAGGAACAGGTTGTTTATTAACTTCAGTAATGGGAGCATTTGTGGCAGTTGAACAAGATTATG
TAAAAGCAGCGGTTGCTGCATTAACGTTTTATGGAGTAGCAGCGGAGATTGCTGCGAGTAAAACAGTAGATCAGGGACCA
GGCAGTTTCCAAGTAGAATTCTTAAATCAATTAGCAAATGTTACCGTAAGCGATATTGAACAATATGGAAAAGCAGAAGA
AGTTCGTTAG

Upstream 100 bases:

>100_bases
GCGTAGGGAGAGTGTAAAAAAACACATGTTCAGGCACTTTGCATACGCGAAGTGTCTTTTTGTGTATCTCCCATTACGGA
AACGAGGAGGAGATGAAACA

Downstream 100 bases:

>100_bases
GGAGATGAGAGAGATGGCACGCATTGAAACAGAAAAGATGTCTCAGTTATTACAAGTATATTTTATTATGGGGAGCAATA
ACTGTCACAAGGGTCCATTA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MHITEIAKVVEQVRKTNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAMLIA
GKAANQQHVPVLFDPVGAGATLYRTEVARHIPNEIKLAMIRGNAAEIANVIHEKWEIKGVDAGTGNGDSVAIATKAANKL
GTVAVITGKEDIVTDGKRTVIIRNGHPILTKVTGTGCLLTSVMGAFVAVEQDYVKAAVAALTFYGVAAEIAASKTVDQGP
GSFQVEFLNQLANVTVSDIEQYGKAEEVR

Sequences:

>Translated_269_residues
MHITEIAKVVEQVRKTNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAMLIA
GKAANQQHVPVLFDPVGAGATLYRTEVARHIPNEIKLAMIRGNAAEIANVIHEKWEIKGVDAGTGNGDSVAIATKAANKL
GTVAVITGKEDIVTDGKRTVIIRNGHPILTKVTGTGCLLTSVMGAFVAVEQDYVKAAVAALTFYGVAAEIAASKTVDQGP
GSFQVEFLNQLANVTVSDIEQYGKAEEVR
>Mature_269_residues
MHITEIAKVVEQVRKTNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAGALVLNMGTLRPDEVEAMLIA
GKAANQQHVPVLFDPVGAGATLYRTEVARHIPNEIKLAMIRGNAAEIANVIHEKWEIKGVDAGTGNGDSVAIATKAANKL
GTVAVITGKEDIVTDGKRTVIIRNGHPILTKVTGTGCLLTSVMGAFVAVEQDYVKAAVAALTFYGVAAEIAASKTVDQGP
GSFQVEFLNQLANVTVSDIEQYGKAEEVR

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=252, Percent_Identity=39.6825396825397, Blast_Score=159, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=276, Percent_Identity=35.8695652173913, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_BACCN (A7GKR3)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001373716.1
- ProteinModelPortal:   A7GKR3
- SMR:   A7GKR3
- STRING:   A7GKR3
- EnsemblBacteria:   EBBACT00000038840
- GeneID:   5344837
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_0363
- eggNOG:   COG2145
- GeneTree:   EBGT00050000002080
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   BCER315749:BCER98_0363-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28297; Mature: 28297

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: BINDING 45-45 BINDING 121-121 BINDING 167-167 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITEIAKVVEQVRKTNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAG
CCHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNMGTLRPDEVEAMLIAGKAANQQHVPVLFDPVGAGATLYRTEVARHIPNEIKLAMI
HHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
RGNAAEIANVIHEKWEIKGVDAGTGNGDSVAIATKAANKLGTVAVITGKEDIVTDGKRTV
ECCHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHEECCCEEE
IIRNGHPILTKVTGTGCLLTSVMGAFVAVEQDYVKAAVAALTFYGVAAEIAASKTVDQGP
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GSFQVEFLNQLANVTVSDIEQYGKAEEVR
CCCHHHHHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MHITEIAKVVEQVRKTNPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMASIAG
CCHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHH
ALVLNMGTLRPDEVEAMLIAGKAANQQHVPVLFDPVGAGATLYRTEVARHIPNEIKLAMI
HHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
RGNAAEIANVIHEKWEIKGVDAGTGNGDSVAIATKAANKLGTVAVITGKEDIVTDGKRTV
ECCHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHEECCCEEE
IIRNGHPILTKVTGTGCLLTSVMGAFVAVEQDYVKAAVAALTFYGVAAEIAASKTVDQGP
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GSFQVEFLNQLANVTVSDIEQYGKAEEVR
CCCHHHHHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA