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The map label for this gene is thiE

Identifier: 152974200

GI number: 152974200

Start: 418425

End: 419078

Strand: Direct

Name: thiE

Synonym: Bcer98_0364

Alternate gene names: 152974200

Gene position: 418425-419078 (Clockwise)

Preceding gene: 152974199

Following gene: 152974201

Centisome position: 10.24

GC content: 38.84

Gene sequence:

>654_bases
ATGGCACGCATTGAAACAGAAAAGATGTCTCAGTTATTACAAGTATATTTTATTATGGGGAGCAATAACTGTCACAAGGG
TCCATTACAAGTAATGAAAGAAGCACTAGATGGTGGCATTACCCTTTTTCAATTTCGGGAAAAAGGAGAAGGAGCTCTAA
CTGGAGAGAAAAGAGTCCAATTTGCCAAACAGTTACAAGCGCTATGTAGGGAGTATCGAGTTCCTTTTATTGTGAATGAT
GATGTGGATTTAGCGCTTAAGCTGGATGCAGACGGTGTTCATGTTGGACAAGATGATGAAGGAATTGAAGTTGTCCGTGA
AAAAATGGGCGATAAAATTGTTGGAGTGTCTGCTCATACAATAGAAGAAGCGCATTTTGCCATTGCAAATGGTGCGGATT
ATTTAGGAATAGGCCCTATTTTTCCAACAAACACAAAAAAAGATACAAAAGCAGTTCAAGGATTAAATGGATTACGTTAT
TTTCGGGAAAAAGGTATTAATATACCGATAGTTGGAATCGGTGGAATTACAATCGAAAATGCTTCTTCAGTAATAGAAGC
AGGAGCAGATGGTGTTTCGGTTATTTCAGCCATTAGTTTGGCGAGTTCAGCCTTTGACAGTGCGAAAGCATTCGTGCATC
AAGTGAAGAAGTAA

Upstream 100 bases:

>100_bases
AGTTTCCAAGTAGAATTCTTAAATCAATTAGCAAATGTTACCGTAAGCGATATTGAACAATATGGAAAAGCAGAAGAAGT
TCGTTAGGGAGATGAGAGAG

Downstream 100 bases:

>100_bases
AGATGGGGATTGAACGGTTGTGTTCTCATCTTACATGAGAAAACGATGGAGTTAATGGAAGTATTTAAAACATATAATGT
GGAGTGCAAATGTATTGTAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MARIETEKMSQLLQVYFIMGSNNCHKGPLQVMKEALDGGITLFQFREKGEGALTGEKRVQFAKQLQALCREYRVPFIVND
DVDLALKLDADGVHVGQDDEGIEVVREKMGDKIVGVSAHTIEEAHFAIANGADYLGIGPIFPTNTKKDTKAVQGLNGLRY
FREKGINIPIVGIGGITIENASSVIEAGADGVSVISAISLASSAFDSAKAFVHQVKK

Sequences:

>Translated_217_residues
MARIETEKMSQLLQVYFIMGSNNCHKGPLQVMKEALDGGITLFQFREKGEGALTGEKRVQFAKQLQALCREYRVPFIVND
DVDLALKLDADGVHVGQDDEGIEVVREKMGDKIVGVSAHTIEEAHFAIANGADYLGIGPIFPTNTKKDTKAVQGLNGLRY
FREKGINIPIVGIGGITIENASSVIEAGADGVSVISAISLASSAFDSAKAFVHQVKK
>Mature_216_residues
ARIETEKMSQLLQVYFIMGSNNCHKGPLQVMKEALDGGITLFQFREKGEGALTGEKRVQFAKQLQALCREYRVPFIVNDD
VDLALKLDADGVHVGQDDEGIEVVREKMGDKIVGVSAHTIEEAHFAIANGADYLGIGPIFPTNTKKDTKAVQGLNGLRYF
REKGINIPIVGIGGITIENASSVIEAGADGVSVISAISLASSAFDSAKAFVHQVKK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=175, Percent_Identity=34.8571428571429, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=194, Percent_Identity=36.5979381443299, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_BACCN (A7GKR4)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001373717.1
- ProteinModelPortal:   A7GKR4
- SMR:   A7GKR4
- STRING:   A7GKR4
- EnsemblBacteria:   EBBACT00000039246
- GeneID:   5344839
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_0364
- eggNOG:   COG0352
- GeneTree:   EBGT00050000001882
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   PRK00043
- BioCyc:   BCER315749:BCER98_0364-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23406; Mature: 23275

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIETEKMSQLLQVYFIMGSNNCHKGPLQVMKEALDGGITLFQFREKGEGALTGEKRVQ
CCCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHH
FAKQLQALCREYRVPFIVNDDVDLALKLDADGVHVGQDDEGIEVVREKMGDKIVGVSAHT
HHHHHHHHHHHCCCCEEECCCCCEEEEECCCCEECCCCCCHHHHHHHHHCCEEECCCHHH
IEEAHFAIANGADYLGIGPIFPTNTKKDTKAVQGLNGLRYFREKGINIPIVGIGGITIEN
HHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEEEEECCEEECC
ASSVIEAGADGVSVISAISLASSAFDSAKAFVHQVKK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARIETEKMSQLLQVYFIMGSNNCHKGPLQVMKEALDGGITLFQFREKGEGALTGEKRVQ
CCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHH
FAKQLQALCREYRVPFIVNDDVDLALKLDADGVHVGQDDEGIEVVREKMGDKIVGVSAHT
HHHHHHHHHHHCCCCEEECCCCCEEEEECCCCEECCCCCCHHHHHHHHHCCEEECCCHHH
IEEAHFAIANGADYLGIGPIFPTNTKKDTKAVQGLNGLRYFREKGINIPIVGIGGITIEN
HHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEEEEECCEEECC
ASSVIEAGADGVSVISAISLASSAFDSAKAFVHQVKK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA